共查询到20条相似文献,搜索用时 9 毫秒
1.
Fuli Zhang Mingyi Wang Dingan Han Haishu Tan Guojian Yang Yaguang Zeng 《Journal of biophotonics》2018,11(2)
We present an in vivo lab‐free full‐field functional optical hemocytometer (FFOH) for application to the capillaries of a live biological specimen, based on the absorption intensity fluctuation modulation (AIFM) effect. Because of the absorption difference between the red blood cells (RBCs) and background tissue under low‐coherence light illumination, an endogenous instantaneous intensity fluctuation is generated by the AIFM effect when RBCs discontinuously traverse the capillary. The AIFM effect is used to highlight the RBC signal relative to the background tissue by computing the real‐time modulation depth. FFOH can simultaneously provide a flow video, the flow velocity and the RBC count. Ourexperimental results can potentially be applied to study the physiological mechanisms of the blood circulation systems of near‐transparent live biological samples. 相似文献
2.
Back Cover: Prolonged in vivo functional assessment of the mouse oviduct using optical coherence tomography through a dorsal imaging window (J. Biophotonics 5/2018) 下载免费PDF全文
Optical coherence tomography through an implanted dorsal imaging window allows for prolonged in vivo structural and functional assessment of the mouse oviduct (Fallopian tube), including threedimensional structural imaging, quantitative measurements of the smooth muscle contraction, and mapping of cilia beat frequency. This method brings new opportunities for live studies and longitudinal analyses of mouse reproductive events in the native context. Further details can be found in the article by Shang Wang et al. ( e201700316 ).
3.
《Journal of biophotonics》2019,12(5)
A large‐depth‐of‐field full‐field optical angiography (LD‐FFOA) method is developed to expand the depth‐of‐field (DOF). The contrast pyramid fusion algorithm is used to fuse 10 FFOA images at different focus depth. Cover images of mouse ear shows LD‐FFOA image has higher contrast and more detailed features. The LD‐FFOA method solves the defocused problem caused by the limited DOF of lens, the curved surface and uneven thickness of the sample. Further details can be found in the article by Mingyi Wang, Nanshou Wu, Hongheng Huang, et al. ( e201800329 ).
4.
Back Cover: Joint tagging assisted fluctuation nanoscopy enables fast high‐density super‐resolution imaging (J. Biophotonics 9/2018) 下载免费PDF全文
Based on multicolor quantum dots (QDs) labeling, the joint tagging assisted super‐resolution radial fluctuation (JT‐SRRF) nanoscopy achieves high‐fidelity super‐resolution imaging of subcellular microtubules and fast live‐cell parallel tracking of cholera toxin subunit B (CTB) induced lipid clusters spatially distributed below the optical diffraction limit. This method paves the way for fast high‐density parallel tracking, which is especially beneficial for the investigation of the intensive dynamics in live‐cell applications. Further details can be found in the article by Zhiping Zeng, Jing Ma, Peng Xi, and Canhua Xu ( e201800020 ).
5.
Inside Front Cover: Enhanced volumetric imaging in 2‐photon microscopy via acoustic lens beam shaping (J. Biophotonics 2/2018) 下载免费PDF全文
Simonluca Piazza Paolo Bianchini Colin Sheppard Alberto Diaspro Martí Duocastella 《Journal of biophotonics》2018,11(2)
Two‐photon microscopy is the tool of choice for fluorescence imaging of deep tissues with high resolution, but can be limited in three‐dimensional acquisition speed and penetration depth. In this work, these issues are addressed by using an acoustic optofluidic lens capable of ultrafast beam shaping on a pixel basis. Driving the lens with different phase profiles enables high‐speed volumetric imaging, or enhanced signal‐to‐background for deeper penetration. Further details can be found in the article by Simonluca Piazza et al. ( e201700050 )
6.
Inside Cover: Polarimetric imaging of biological tissues based on the indices of polarimetric purity (J. Biophotonics 4/2018) 下载免费PDF全文
Albert Van Eeckhout Angel Lizana Enric Garcia‐Caurel José J. Gil Adrià Sansa Carla Rodríguez Irene Estévez Emilio González Juan C. Escalera Ignacio Moreno Juan Campos 《Journal of biophotonics》2018,11(4)
The cover shows the image enhancement of biological tissues provided by the Indices of Polarimetric Purity (IPPs). By measuring the Mueller matrix of a biological sample, using an imaging polarimeter, the IPPs are calculated. They are polarimetric indicators providing further synthetization of depolarizing samples and leading to enhanced image contrast for some biological structures. Once the IPPs are calculated, a pseudo‐colouring technique is applied for higher visualization. Further details can be found in the article by Albert Van Eeckhout et al. ( e201700189 )
7.
Front Cover: A high‐throughput all‐optical laser‐scanning imaging flow cytometer with biomolecular specificity and subcellular resolution (J. Biophotonics 2/2018) 下载免费PDF全文
A new type of high‐throughput imaging flow cytometer (>20 000 cells s‐1) based upon an all‐optical ultrafast laser‐scanning imaging technique, called free‐space angular‐chirp‐enhanced delay (FACED) is reported. FACED imaging flow cytometers enables high‐throughput visualization of functional morphology of individual cells with subcellular resolution. It critically empowers largescale and deep characterization of single cells and their heterogeneity with high statistical power— an ability to become increasingly critical in single‐cell analysis adopted in a wide range of biomedical and life‐science applications. Further details can be found in the article by Wenwei Yan et al. ( e201700178 )
8.
Inside Back Cover: Multiphoton dynamic imaging of the effect of chronic hepatic diseases on hepatobiliary metabolism in vivo (J. Biophotonics 9/2018) 下载免费PDF全文
Chih‐Ju Lin Sheng‐Lin Lee Wei‐Hsiang Wang Vladimir A. Hovhannisyan Yao‐De Huang Hsuan‐Shu Lee Chen‐Yuan Dong 《Journal of biophotonics》2018,11(9)
In vivo multiphoton imaging was used to map changes in hepatobiliary metabolism in liver fibrosis (left column) and hepatocellular carcinoma (right column). The top row shows the maps of kinetic rate constant of the uptake and esterase processing while the bottom row shows that of bile canalicular excretion of xenobiotics. Further details can be found in the article by Chih‐Ju Lin, Sheng‐Lin Lee, Wei‐Hsiang Wang, et al. ( e201700338 ).
9.
《Journal of biophotonics》2019,12(4)
Monitoring the blood‐brain barrier (BBB) permeability plays a key role in assessing drug release with high resolution. In this work, with the help of optical clearing skull window, we not only realized non‐invasive BBB opening by photodynamic therapy, but also developed a method based on spectral‐imaging to in vivo dynamically monitor the changes in BBB permeability. Further details can be found in the article by Wei Feng, Chao Zhang, Tingting Yu, et al. ( e201800330 ).
10.
Inside Cover: Fiber‐based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs (J. Biophotonics 9/2018) 下载免费PDF全文
Alba Alfonso‐Garcia Jeny Shklover Benjamin E. Sherlock Alyssa Panitch Leigh G. Griffiths Laura Marcu 《Journal of biophotonics》2018,11(9)
Tissue autofluorescence provides fluorescence lifetime contrast between acellular tissue and that containing newly seeded cells. Fiber‐based fluorescence lifetime imaging (FLIm) can be used for tracking recellularization of engineered vascular grafts and potential matrix remodeling at large scale, without compromising sample integrity. FLIm cellular contrast was verified in a subset of samples seeded with eGFP‐labelled cells. Results suggests fiberbased FLIm is a suitable tool for monitoring recellularization of engineered tissue nondestructively. Further details can be found in the article by Alba Alfonso‐Garcia, Jeny Shklover, Benjamin E. Sherlock, et al. ( e201700391 ).
11.
Front Cover: Spectrally encoded coherence tomography and reflectometry: Simultaneous en face and cross‐sectional imaging at 2 gigapixels per second (J. Biophotonics 4/2018) 下载免费PDF全文
SECTR is a novel multimodal imaging platform for combined volumetric optical coherence tomography (OCT) and en face spectrally encoded reflectometry (SER). The authors demonstrate three‐dimensional motion‐tracking with millisecond temporal and micron spatial resolution using complementary data from OCT and SER, and preliminary algorithms and results showing real‐time image aiming and multi‐volumetric mosaicking for reconstruction of wide‐field composites. The image shows a noninvasively imaged nine‐field mosaic of in vivo human retina and depth‐resolved visualization of tissue microstructures. Further details can be found in the article by Mohamed T. El‐Haddad, Ivan Bozic, and Yuankai K. Tao ( e201700268 )
12.
《Journal of biophotonics》2019,12(11)
Thrombosis monitoring in vivo in small animals is of great value in basic research. The aim of this study is to utilize OCT to monitor thrombosis progression in femoral vein of mice from various measurement criteria, and to validate its use in evaluation the efficacy of the antithrombotic drug. The proved capability of obtaining thrombodynamics information in mice model provide valuable use in preclinical studies for anti‐thrombotic drugs development research. Further details can be found in the article by Yao Yu, Menghan Yu, Jian Liu, et al. ( e201900105 ).
13.
Inside Back Cover: Development of a 3‐dimensional tissue lung phantom of a preterm infant for optical measurements of oxygen—Laser‐detector position considerations (J. Biophotonics 3/2018) 下载免费PDF全文
Jim Larsson Peilang Liao Patrik Lundin Emilie Krite Svanberg Johannes Swartling Märta Lewander Xu Joakim Bood Stefan Andersson‐Engels 《Journal of biophotonics》2018,11(3)
The picture depicts the different 3d‐printed organs, thorax, lungs, heart and bone. Assembled it is used as an optical phantom of a preterm infant for performing percutaneous optical measurements of the gas content in the lungs. In order to simulate the optical properties of the tissue, the heart and thorax can be filled with liquid phantoms, a mixture of Intralipid and Indian Ink. Further details can be found in the article by Jim Larsson et al. ( e201700097 ).
14.
Back Cover: Non‐invasive optical method for real‐time assessment of intracorneal riboflavin concentration and efficacy of corneal cross‐linking (J. Biophotonics 7/2018) 下载免费PDF全文
Giuseppe Lombardo Valentina Villari Norberto L. Micali Nancy Leone Cristina Labate Maria P. De Santo Marco Lombardo 《Journal of biophotonics》2018,11(7)
We disclose a theranostic device for performing image‐guided riboflavin/UV‐A corneal cross‐linking. The device determines treatment efficacy by real time monitoring of riboflavin concentration in the corneal stroma. The study shows efficacy of the device in eye bank human donor tissues. Further details can be found in the article by Giuseppe Lombardo et al. ( e201800028 )
15.
Inside Front Cover: Near‐infrared bone densitometry: A feasibility study on distal radius measurement (J. Biophotonics 7/2018) 下载免费PDF全文
This study provides a simple method to detect human distal radius bone density based on near infrared (NIR) imaging. The information of bone mineral density can be measured by transluminational optical bone densitometric system. Compared to dual‐energy x‐ray absorptiometry (DXA) results in clinical trial, NIR images show a strong correlation to DXA. Further details can be found in the article by Chun Chung, Yu‐Pin Chen, Tsai‐Hsueh Leu, and Chia‐Wei Sun ( e201700342 ).
16.
《Journal of biophotonics》2019,12(7)
How does the ischemic tissue re‐vascularize? Now we can visualize the reperfusion process at high spatial resolution by using a dual‐wavelength MEMS scanning based optical resolution photoacoustic microscopy (OR‐PAM) system. The fast imaging capability enables continuous monitoring of skin reperfusion in a mouse model. It's also found that the ischemic tissue has a significantly higher oxygen consumption rate in the reperfusion stage comparing to the normal tissue. Further details can be found in the article by Renzhe Bi, U.S. Dinish, Chi Ching Goh, et al. ( e201800454 ).
17.
Back Cover: Autofluorescence and white light imaging‐guided endoscopic Raman and diffuse reflectance spectroscopy for in vivo nasopharyngeal cancer detection (J. Biophotonics 4/2018) 下载免费PDF全文
Jianji Pan Zhihong Xu Rong Chen Shangyuan Feng Guannan Chen Yongzeng Li Michael Short Jianhua Zhao Yasser Fawzy Haishan Zeng 《Journal of biophotonics》2018,11(4)
An integrated 4‐modality endoscopy system combining white light imaging, autofluorescence imaging, diffuse reflectance spectroscopy and Raman spectroscopy technologies was developed for in vivo endoscopic nasopharyngeal cancer detection. Both high diagnostic sensitivity (98.6%) and high specificity (95.1%) for differentiating cancer from normal tissue sites were achieved using this system combined with multivariate diagnostic algorithm, demonstrating great potential for improving real‐time, in vivo diagnosis of cancer at endoscopy. Further details can be found in the article by Duo Lin et al. ( e201700251 )
18.
Inside Cover: Optimized perfusion‐based CUBIC protocol for the efficient whole‐body clearing and imaging of rat organs (J. Biophotonics 5/2018) 下载免费PDF全文
Paweł Matryba Lukasz Bozycki Monika Pawłowska Leszek Kaczmarek Marzena Stefaniuk 《Journal of biophotonics》2018,11(5)
Optical tissue clearing is a method allowing post‐mortem deep imaging of organs in three dimensions. By optimizing the CUBIC clearing protocol, the authors provide rapid and simple approach to clear the entire adult rat organism within as little as four days, which is accompanied by the variety of its staining and imaging techniques. The image was captured with polarizers and demonstrates transparent rodent heart with thread‐like crystals of clearing reagent. Further details can be found in the article by Pawe? Matryba et al. ( e201700248 ).
19.
《Journal of biophotonics》2019,12(5)
A fast polarization‐resolved second harmonic generation microscope is implemented to map collagen orientation in thick and deforming tissues during mechanical assays. This system is based on line‐to‐line switching of the laser polarization using an electro‐optical modulator and works in epi‐detection geometry. After proper calibration, it successfully highlights the collagen dynamic alignment along the traction direction in ex vivo murine skin dermis. Further details can be found in the article by Guillaume Ducourthial, Jean‐Sébastien Affagard, Margaux Schmeltz, et al. ( e201800336 ).
20.
《Journal of biophotonics》2019,12(4)
Hyperspectral scanning laser optical tomography is developed to provide spectrally resolved volume data sets with high spectral resolution for large mesoscopic samples. It can be used to resolve largely overlapping fluorophores, as demonstrated by the 3D fluorescence hyperspectral reconstruction of a dual‐labelled mouse thymus gland sample and to distinguish between signals from autofluorescence of diseased and normal tissue without prior knowledge. Further details can be found in the article by Lingling Chen, Guiye Li, Li Tang, et al. ( e201800221 ).