共查询到20条相似文献，搜索用时 15 毫秒
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Yoshiki Tani Toru Nagasawa Hidenori Sugisaki Koichi Ogata 《Bioscience, biotechnology, and biochemistry》2013,77(6):1287-1294
The Cholinesterase of Pseudomonas aeruginosa A–16 was purified approximately 11,150-fold with an overall recovery of 15.2% and proved to be homogeneous by electrophoresis, ultracentrifugation and chromatography. The molecular weight of the enzyme was determined as approximately 30,000 by equilibrium centrifugation and gel filtration methods. The sedimentation coefficient, S20,w was determined to be 3.3 S. Isoelectric focusing electrophoresis with carrier ampholite revealed that the enzyme had an isoelectric point around pH 8.1.The purified Cholinesterase, which was considered to be an acetylcholinesterase from its substrate specificity, hydrolyzed acetylthiocholine and acetylcholine at the highest rates among the various esters tested.The estimated values of Km at pH 7.5 and 25°C were 1.5 × 10?4 m for acetylthiocholine and 1.9 × 10?4 m for acetylcholine. The enzyme also hydrolyzed the acetyl and propionyl esters of several aliphatic and aromatic alcohols at a lower rate which was entirely dependent on the properties of the alcohol moiety of those esters. 相似文献
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Chenyang Li Tianze Zhang Yu Liu Zongdi Li Yaqin Wang Shuai Fu Yi Xu Tong Zhou Jianxiang Wu Xueping Zhou 《Molecular Plant Pathology》2022,23(3):447-458
The unfolded protein response (UPR) plays important roles in plant virus infection. Our previous study has proved that rice stripe virus (RSV) infection elicits host UPR. However, the mechanism on how the UPR is triggered upon RSV infection remains obscure. Here, we show that the bZIP17/28 branch of the UPR signalling pathway is activated upon RSV infection in Nicotiana benthamiana. We found that membrane-associated proteins NSvc2 and NSvc4 encoded by RSV are responsible for the activation of the bZIP17/28 branch. Ectopic expression of NSvc2 or NSvc4 in plant leaves induced the proteolytic processing of NbbZIP17/28 and up-regulated the expression of UPR-related genes. Silencing NbbZIP17/28 significantly inhibited RSV infection. We show that RSV can specifically elicit the UPR through the bZIP17/28 branch, thus promoting virus infection of N. benthamiana plants. 相似文献
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Masato Nakamura Mamoru Nozaki Yuji Iwata Nozomu Koizumi Yasushi Sato 《Plant Biotechnology》2022,39(2):129
Endoplasmic reticulum (ER) stress activates unfolded protein responses (UPRs), such as promoting protein folding under the control of specific gene expression. Our previous study showed that ER stress induced by ER stress inducers such as tunicamycin (Tm), an inhibitor of N-linked glycan synthesis, causes ectopic lignin deposition in Arabidopsis roots, but the relationship between UPR and ectopic lignin deposition remains unclear. The receptor-like kinase THESEUS1 (THE1) has been shown to sense cell wall damage (CWD) induced in Arabidopsis by cellulose synthase inhibitors such as isoxaben (ISO) and to activate ectopic lignin deposition. In this study, we assessed the involvement of THE1 in ectopic lignin deposition caused by the ER stress inducer Tm. The loss-of-function mutation of THE1, the1-3, suppressed Tm-induced root growth inhibition and ectopic lignin deposition, revealing that THE1 is involved in root growth defects and ectopic lignin deposition caused by ER stress. Similarly, ISO treatment induced ectopic lignin deposition as well as the expression of the UPR marker genes binding protein 3 (BiP3) and ER-localized DnaJ 3b (ERdj3b). Conversely, in the the1-3 mutant, ISO-induced ectopic lignin deposition and the expression of BiP3 and ERdj3b were suppressed. These results showed that THE1 is involved in not only root growth inhibition and ectopic lignin deposition caused by ER stress but also CWD-induced UPR. 相似文献
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Chlorophylls are essential for photosynthesis. Chlorophyll biosynthesis is catalyzed by a series of enzyme complexes, such as Mg protoporphyrin IX methyltransferase. A yellow mutant of Arabidopsis was isolated using an enthyl methane sulfonate (EMS) mutagenesis strategy. Chlorophyll content dramatically reduced and grana stacking was absent in the mutant. Genetic analysis indicated that the mutant was controlled by a single recessive gene. Using map based cloning strategy, the gene responsible for the mutant phenotype was mapped to a region of 114 kb between the molecular markers F13M23 and T30C3 on chromosome 4, in which the CHLM gene encoding Mg protoporphyrin IX methyltransferase was included. The mutant was proved to be an allelic mutant of CHLM gene by sequencing and allelism test and then was designated as chlm 4. Gly59 of CHLM was replaced by Glu59 in chlm 4, which indicated that Gly59 was essential for the function of Mg protoporphyrin IX methyltransferase. 相似文献
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Kumar R Selth LA Schulz RB Tay BS Neilsen PM Callen DF 《Journal of cellular biochemistry》2011,112(10):2742-2747
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采用实时荧光定量RT-PCR和Northern blotting技术检测了野生型拟南芥中CBP60g基因对丁香假单胞菌和非生物胁迫的响应,并对丁香假单胞菌接种后,野生型拟南芥、cbp60g-1突变体和CBP60g过表达转基因植物中抗逆相关基因的表达变化进行检测。结果显示:(1)在野生型拟南芥中CBP60g基因的表达能被丁香假单胞菌、高盐、冷和机械损伤所诱导。(2)经丁香假单胞菌诱导后病程相关基因PR5和AIG1的表达在过表达转基因植物中明显高于野生型。(3)受干旱和ABA诱导的AtMYB2基因的表达在过表达转基因植物中也高于野生型。研究表明,CBP60g同时参与了拟南芥对生物和非生物胁迫响应。 相似文献
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Brandon J. Reeder Michael A. Hough 《Acta Crystallographica. Section D, Structural Biology》2014,70(5):1411-1418
Plant nonsymbiotic haemoglobins fall into three classes, each with distinct properties but all with largely unresolved physiological functions. Here, the first crystal structure of a class 3 nonsymbiotic plant haemoglobin, that from Arabidopsis thaliana, is reported to 1.77 Å resolution. The protein forms a homodimer, with each monomer containing a two‐over‐two α‐helical domain similar to that observed in bacterial truncated haemoglobins. A novel N‐terminal extension comprising two α‐helices plays a major role in the dimer interface, which occupies the periphery of the dimer–dimer face, surrounding an open central cavity. The haem pocket contains a proximal histidine ligand and an open sixth iron‐coordination site with potential for a ligand, in this structure hydroxide, to form hydrogen bonds to a tyrosine or a tryptophan residue. The haem pocket appears to be unusually open to the external environment, with another cavity spanning the entrance of the two haem pockets. The final 23 residues of the C‐terminal domain are disordered in the structure; however, these domains in the functional dimer are adjacent and include the only two cysteine residues in the protein sequence. It is likely that these residues form disulfide bonds in vitro and it is conceivable that this C‐terminal region may act in a putative complex with a partner molecule in vivo. 相似文献
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根据灰岩皱叶报春PF sHSP 17-1热激蛋白基因序列,采用PCR法从差减文库中克隆得到该基因,并构建pCAMBIA1301-PF sHSP 17-1植物表达载体,通过根癌农杆菌介导的花器浸染法转化拟南芥,经潮霉素筛选、PCR和RT-PCR法鉴定后,对转基因植株和野生型对照进行高温胁迫,分析其耐热性差异.结果显示,在40℃、42℃、44℃处理下,转基因拟南芥的相对电导率和丙二醛含量升高的幅度均显著低于野生型对照,而脯氨酸含量的积累明显高于对照,可溶性蛋白的含量在热胁迫下也比对照稳定,仅有轻微下降.试验证明,PF sHSP 17-1基因对植物的耐热性有重要作用. 相似文献