共查询到20条相似文献,搜索用时 15 毫秒
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Luca Madaro Fabrizio Antonangeli Annarita Favia Bianca Esposito Filippo Biamonte Marina Bouché Elio Ziparo Gigliola Sica Antonio Filippini Alessio D'Alessio 《Journal of cellular biochemistry》2013,114(8):1843-1851
Caveolin‐1 (CAV1) is the principal structural component of caveolae which functions as scaffolding protein for the integration of a variety of signaling pathways. In this study, we investigated the involvement of CAV1 in endothelial cell (EC) functions and show that siRNA‐induced CAV1 silencing in the human EC line EA.hy926 induces distinctive morphological changes, such as a marked increase in cell size and formation of stress fibers. Design‐based stereology was employed in this work to make unbiased quantification of morphometric properties such as volume, length, and surface of CAV1 silenced versus control cells. In addition, we showed that downregulation of CAV1 affects cell cycle progression at G1/S phase transition most likely by perturbation of AKT signaling. With the aim to assess the contribution of CAV1 to typical biological processes of EC, we report here that CAV1 targeting affects cell migration and matrix metalloproteinases (MMPs) activity, and reduces angiogenesis in response to VEGF, in vitro. Taken together our data suggest that the proper expression of CAV1 is important not only for maintaining the appropriate morphology and size of ECs but it might represent a prospective molecular target for studying key biological mechanisms such as senescence and tumorigenesis. J. Cell. Biochem. 114: 1843–1851, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
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Natasha Baker Guofeng Zhang Yang You Rocky S. Tuan 《Journal of cellular biochemistry》2012,113(12):3773-3787
Caveolin‐1 is a scaffolding protein of cholesterol‐rich caveolae lipid rafts in the plasma membrane. In addition to regulating cholesterol transport, caveolin‐1 has the ability to bind a diverse array of cell signaling molecules and regulate cell signal transduction in caveolae. Currently, there is little known about the role of caveolin‐1 in stem cells. It has been reported that the caveolin‐1 null mouse has an expanded population of cells expressing stem cell markers in the gut, mammary gland, and brain, suggestive of a role for caveolin‐1 in stem cell regulation. The caveolin‐1 null mouse also has increased bone mass and an increased bone formation rate, and its bone marrow‐derived mesenchymal stem cells (MSCs) have enhanced osteogenic potential. However, the role of caveolin‐1 in human MSC osteogenic differentiation remains unexplored. In this study, we have characterized the expression of caveolin‐1 in human bone marrow derived MSCs. We show that caveolin‐1 protein is enriched in density gradient‐fractionated MSC plasma membrane, consisting of ~100 nm diameter membrane‐bound vesicles, and is distributed in a punctate pattern by immunofluoresence localization. Expression of caveolin‐1 increases in MSCs induced to undergo osteogenic differentiation, and siRNA‐mediated knockdown of caveolin‐1 expression enhances MSC proliferation and osteogenic differentiation. Taken together, these findings suggest that caveolin‐1 normally acts to regulate the differentiation and renewal of MSCs, and increased caveolin‐1 expression during MSC osteogenesis likely acts as a negative feedback to stabilize the cell phenotype. J. Cell. Biochem. 113: 3773–3787, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
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MicroRNA‐155 Regulates ROS Production,NO Generation,Apoptosis and Multiple Functions of Human Brain Microvessel Endothelial Cells Under Physiological and Pathological Conditions 下载免费PDF全文
Yajing Liu Qunwen Pan Yuhui Zhao Caixia He Kexia Bi Yusen Chen Bin Zhao Yanfang Chen Xiaotang Ma 《Journal of cellular biochemistry》2015,116(12):2870-2881
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Microenvironmental Interaction Between Hypoxia and Endothelial Cells Controls the Migration Ability of Placenta‐Derived Mesenchymal Stem Cells via α4 Integrin and Rho Signaling 下载免费PDF全文
Jong Ho Choi Seung Mook Lim Yong In Yoo Jieun Jung Jong‐Won Park Gi Jin Kim 《Journal of cellular biochemistry》2016,117(5):1145-1157
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Long Non‐Coding RNA MALAT1 Promotes Proliferation,Angiogenesis, and Immunosuppressive Properties of Mesenchymal Stem Cells by Inducing VEGF and IDO 下载免费PDF全文
Xiujun Li Yuxian Song Fei Liu Dan Liu Huishuang Miao Jing Ren Jingjing Xu Liang Ding Yali Hu Zhiqun Wang Yayi Hou Guangfeng Zhao 《Journal of cellular biochemistry》2017,118(9):2780-2791
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Pelin Esma Emirbayer Ankit Sinha Vladimir Ignatchenko Stefanie Hoyer Jan Dörrie Niels Schaft Monika Pischetsrieder Thomas Kislinger 《Proteomics》2017,17(21)
The histamine receptors (HRs) represent a subclass of G protein‐coupled receptors (GPCRs) and comprise four subtypes. Due to their numerous physiological and pathological effects, HRs are popular drug targets for the treatment of allergic reactions or the regulation of gastric acid secretion. Hence, an understanding of the functional selectivity of HR ligands has gained importance. These ligands can bind to specific GPCRs and selectively activate defined pathways. Supporting the activation of a therapeutically necessary pathway without the activation of other signaling cascades can result in drugs with more specific activity and fewer side effects. To evaluate the cellular consequences resulting from receptor binding, comprehensive analyses of cellular protein alterations upon incubation with ligands are required. For this purpose, endothelial cells are treated with histamine, as the endogenous ligand of HRs, to obtain a global overview of its cellular effects. Quantitative proteomics and pathway analyses of histamine‐treated and untreated cells reveal enrichment of the nuclear factor‐κB and tumor necrosis factor signaling pathways, cytokine?cytokine receptor interactions, complement and coagulation cascades, and acute inflammatory processes upon histamine treatment. This strategy offers the opportunity to monitor HR‐mediated signaling in a multidimensional manner. 相似文献
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