Microsatellite markers for Duperrea pavettifolia (Rubiaceae)

? Premise of the study: The development of microsatellite primers for Duperrea pavettifolia will be the foundation for mating system analysis and conservation research. ? Methods and Results: Nineteen microsatellite markers were developed and characterized in two wild populations by using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Polymorphisms were evaluated in 24 individuals from two natural populations. Eleven of these primers generated polymorphic loci. ? Conclusions: These microsatellite markers will be useful in future investigations into the population genetics and mating system of D. pavettifolia.     

玫瑰红链霉菌336质粒（pSR336）DNA的分离及特性研究

从葡萄糖异构酶产生菌──玫瑰红链霉菌３３６中分离得到质粒ｐＳＲ３３６。经电泳检测和电镜观察证实,存在共价闭合超螺旋和开环两种分子构型,分子量约为６．３５ｋｂ,拷贝数约为１３０。采用高温（４０℃）,吖啶橙、溴化乙锭和ＳＤＳ等物理、化学因素消除ｐＳＲ３３６,均未获得质粒消除株,表明ｐＳＲ３３６质粒是非常稳定的。用变铅青链霉菌ＴＫ２１作受体,进行平皿杂交以检测ｐＳＲ３３６的接合转移能力,未观察到麻点（ｐｏｃｋ）的形成。用ＨｉｎｄⅢ分别酶切ｐＳＲ３３６和ｐＩＪ４８６,连接得到一个嵌合质粒ｐＩＲ３０,检测玫瑰红链霉     

Plate 407. Begonia goudotii

The taxonomic history, relationships, distribution, ecology and cultural requirements of Begonia goudotii A.DC., are reviewed. This species, which is endemic to Madagascar and especially notable for its large basal leaves (usually solitary in the wild), is described and floral diagnoses are presented.     

Plate 447. Didymosalpinx norae

Didymosalpinx norae (Swynn.) Keay ( Rubiaceae ) is a shrub native of eastern Africa, with a disjunct distribution. It occurs in the rain forests of the Indian Ocean coastal belt and in submontane forests of the Chimanimani range at the border of Mozambique and Zimbabwe. Living material at the National Botanic Garden in Belgium is used here to illustrate and describe it. Taxonomic history, distribution, habitat and cultivation requirements are also discussed. The affinity of the genus Didymosalpinx raises some questions. The material used here was also included in recent phylogenetic analyses shortly reviewed here; its belonging to the tribe Gardenieae is questionable.