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1.
SUSAN E. HALBERT B. JUNE CONNELLY RICHARD M. LISTER ROBERT E. KLEIN GUY W. BISHOP 《The Annals of applied biology》1992,121(1):123-132
Plants with symptoms of barley yellow dwarf virus (BYDV) obtained in infection feeding assays of aphids collected in the field in Idaho between 1986 and 1988 were tested for virus transmissibility by possible aphid vectors. Isolates obtained during 1987–1988 were also tested with a range of polyclonal antisera which distinguished PAV, MAV, SGV, RPV and RMV serotypes. In 1989 some Idaho (ID) BYDV isolates, maintained as standards for comparison, were serotyped and tested for aphid transmissibility, using 11 species of aphids. There was not always the expected correspondence between serotype and vector specificity for ID isolates. For isolates obtained from field-collected Rhopalosiphum padi, vector transmissibility and serotype corresponded with previous reports; however, 44% of isolates which were serotyped as RMV were also transmissible by species other than Rhopalosiphum maidis. Similarly, the transmissibility of the ID laboratory standards did not always conform to the reported vector specificity of serotypes. The laboratory ID-MAV culture was transmitted by Metopolophium dirhodum and Myzus persicae as well as by Sitobion avenae. The laboratory ID-SGV culture was transmitted by R. padi and 5. avenae as well as by Schizaphis graminum. The ID-RPV culture was transmitted by S. graminum and Rhopalosiphum insertum as well as R. padi. Both of two laboratory ID-RMV cultures were transmissible by R. insertum and R. padi transmitted one of them. The results indicate that, for isolates collected in Idaho, vector specificity cannot be assumed from their serotypes. 相似文献
2.
Biological Characterization of an Italian Isolate of Barley Yellow Dwarf Luteovirus from Barley 总被引:1,自引:0,他引:1
An isolate of BYDV (BYDV-OC), from barley in Northwest Italy with typical symptoms of yellowing and dwarfing, was transmitted by Rhopalosiphum padi, Sitobion fragariae. S. avenae, Metopolophium festucae, R. maidis and M. dirhodum , but not by Myzus persicae or Schizaphis graminum . It reacted in DAS-ELISA with monoclonal and polyclonal antisera to PAV, but not with antibodies to MAV, RPV and RMV. A polyclonal antiserum prepared to BYDV-OC did not react with MAV-like, RPV-like, or RMV-like isolates of BYDV in ELISA or in Western blots. The concentration of BYDVOC in Avena byzantina plants decreased from weeks 1 to 10 after inoculation, but the total virus content per plant increased up to weeks 7 to 8, following the increase of plant weight. 相似文献
3.
Non‐cultivated grass hosts of yellow dwarf viruses in Ethiopia and their epidemiological consequences on cultivated cereals
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Berhanu Bekele Safaa Kumari Seid Ahmed Chemeda Fininsa Abdurazak Yusuf Adane Abraham 《Journal of Phytopathology》2018,166(2):103-115
The yellow dwarf (YD) disease complex epidemics in cultivated cereals grown in a specific period of the year mainly depend on the presence of potential reservoir alternative hosts harbouring both the viruses and the vectors over the off‐season and serve as a source of inoculum in subsequent cropping season, further spread being supported by efficient aphid vectors. As such, an extensive and intensive exploration to generate base line information on the identity and prevalence of YD viruses [barley yellow dwarf virus (BYDV)‐PAV, BYDV‐MAV and BYDV‐SGV; cereal yellow dwarf virus (CYDV)‐RPV; and maize yellow dwarf virus (MYDV)‐RMV] on wild annual and perennial grasses and forage cereals alternative hosts was conducted consecutively during 2013–2015 main‐ and short‐rainy seasons in cereals growing belts of Ethiopia. Random sampling was employed to collect the samples that were tested by the tissue blot immunoassay (TBIA) to identify the YDVs associated with the hosts using a battery of virus‐specific polyclonal antibodies. Of 13,604 samples analysed, YDVs were detected in 392 (2.9%) samples, which consisted of various wild grasses, forage cereals and three cultivated crops. YDVs were identified from at least 26 grass species and forage cereals, some of them are new records, and some are previously documented hosts. To our knowledge, this is the first report of YDV infection of Andropogon abyssinicus (FresenR.Br. ex Fresen.) (BYDV‐PAV), Avena abyssinica Hochst (BYDV‐PAV), Bromus pectinatus Thunb. (BYDV‐PAV and BYDV‐MAV), Eragrostis tef (Zuccagni) Trotter (BYDV‐PAV), Eragrostis sp. (BYDV‐PAV), Hyparrhenia anthistrioides Stapf. (BYDV‐PAV), Panicum coloratum L. (BYDV‐PAV), Polypogon monspeliensis (L.) Desf. (BYDV‐PAV), Setaria pumila (Poir.) Roem & Schult (BYDV‐PAV, BYDV‐SGV and MYDV‐RMV), Setaria australiensis (Scribn. & Merrill) Vickery (BYDV‐PAV, BYDV‐MAV and CYDV‐RPV) and Snowdenia polystachya (Fresen.) Pilg (BYDV‐PAV, BYDV‐MAV, BYDV‐SGV, CYDV‐RPV and MYDV‐RMV). 相似文献
4.
Identification, Distribution and Vector Population Dynamics of Barley Yellow Dwarf Virus in Three Cereal-Producing Areas of Spain 总被引:1,自引:0,他引:1
ELISA-based surveys during 1985–87 in three major cereal-growing areas of Spain confirmed the presence of barley yellow dwarf virus (BYDV). Samples of small grain cereals and grasses with and without BYDV-like symptoms were collected in the central, southwestern, and northeastern Spain. Infections were found in all cereal species sampled and in some grasses. About 37 % of the samples collected in 1985 were infacted with isolates of the PAV serotype. Isolates of the RPV serotype were less common, and were detected only in samples from the central region at El Encin, Madrid. Only a single sample, collected from El Encin in 1987, was unequivocally diagnosed as containing an isolate of the MAV serotype. Aphid vector population dynamics was monitored during fall and winter of 1984–87 in the central region. Rhopalosiphum padi L. appeared to be the most abundant species during fall and winter months, infesting grasses and volunteer wheat. Other species present were Sitobion avenae (F.), Metopolophium dirhodum (Walker) and Rhopalosiphum maidis (Fitch). Both R. padi and S. avenae seem to be anholocyclic in the central region of Spain, and are able to remain and reproduce on wheat volunteers and grasses until the beginning of spring. S, avenae populations increase quickly on wheat volunteers in April, while populations of R. padi remain low. Therefore, spread of S. avenae-transmitted BYDV types to neighbouring cereal fields seem more likely to occur than spread of other types. Other possible virus reservoirs, such as maize, also need investigation for a better understanding of BYDV epidemiology in the central and other cercal-growing areas of Spain. 相似文献
5.
CHENG Zhuomin HE Xiaoyuan WU MaosenZHOU Guanghe(Paul Keese P. M. Waterhouse 《中国科学:生命科学英文版》1996,39(5):534-543
GPV is a Chinese serotype isolate of barley yellow dwarf virus (BYDV) that has no reactionwith antiserum of MAV, PAV, SGV, RPV and RMV. The sequence of the coat protein (CP) of GPV isolate of BYDV was identified and its amino acid sequence was deduced. The coding region for the putative GPV CP is 603 bases nucleotides and encodes a Mr 22218 (22 ku) protein. The same as MAV, PAV and RPV, GPV contained a second ORF within the coat protein coding region. This protein of 17024 Mr (17 ku) is thought to correspond to the Virion protein genome linked (Vpg). Sequence comparisons of the CP coding region between the GPV isolate of BYDV and other isolates of BYDV have been done. The nucleotide and ammo acid sequence homology of GPV has a greater identity to the sequence of RPV than those of PAV and MAV. The GPV CP sequence shared 83.7% of nucleotide similarity and 77.5% of deduced amino add similarity, whereas that of the PAV and MAV shared 56.9%. 53.2% and 44.1%. 43.8% respectively. According to BYDV-GPV CP seque 相似文献
6.
Nadir Naveed Siddiqui Muhammad Ilyas Shahid Mansoor Abid Azhar Muhammad Saeed 《Journal of Phytopathology》2012,160(1):13-18
Barley yellow dwarf virus (BYDVs) is an emerging threat for wheat and may seriously threaten its production, especially as climate change may result in increased infestation by aphids, the insect vectors of the virus. To assess the possibility of using pathogen‐derived resistance against the virus, the genetic diversity of BYDVs originating from different wheat‐growing areas of Pakistan where its incidence has been higher was investigated. Wheat samples with suspected symptoms of BYDVs were screened for the presence of Barley yellow dwarf and Cereal yellow dwarf viruses (B/CYDVs) subgroup 1 (Barley yellow dwarf virus‐PAV, BYDV‐MAV, BYDV‐SGV) and subgroup II (BYDV‐RPV, CYDV‐RPV, BYDV‐GPV) by PCR using basic multiplex oligonucleotides designed on coat protein (CP) of the virus. Of 37 samples tested, 13 were positive for BYDV subgroup I and only one sample was positive for BYDV subgroup II. Samples positive for subgroup I were further tested by PCR, and results showed that 10 samples were positive for BYDV‐PAV and three for BYDV‐MAV. DNA sequences of CP region of nine isolates (BYDV‐PAV) were determined and compared with available sequences in databases. Sequence analysis showed that three isolates (from Fatehjang, Nowshera and Attock districts) had maximum identity (92.8–94.6%) to BYDV‐PAS, and six isolates (from Peshawar, Islamabad Swabi and Faisalabad districts) had maximum identity (99.3–99.7%) to BYDV‐PAV. Thus BYDV‐PAV species may be dominant in northern wheat‐growing areas of Pakistan. The conserved nature of the BYDVs suggests that pathogen‐derived resistance strategies targeting the coat protein of the virus are likely to provide protection under field conditions. 相似文献
7.
Arezoo Pakdel Alireza Afsharifar Ali Niazi Reza Almasi Keramatollah Izadpanah 《Journal of Phytopathology》2010,158(5):357-364
During a survey , 148 wheat, 70 barley and 24 wild grass samples of plants showing symptoms of yellowing or reddening of leaves and general stunting were collected in central and southern provinces of Iran and tested for Barley yellow dwarf virus (BYDV) and Cereal yellow dwarf virus (CYDV) infection by enzyme‐linked immunosorbent assay (ELISA) and tissue print immunoassay (TPIA). The results showed the presence of the viruses in most regions. Positive reactions to BYDV‐PAV, BYDV‐MAV, CYDV‐RPV and BYDV‐SGV antisera were recorded. BYDV‐PAV was the most prevalent virus. The genetic diversity of BYDV‐PAV isolates in central and southern provinces was studied by analysing ORF1 (903 nt) and read through domain (RTD) (575 nt) of 13 and nine isolates respectively. Sequence analysis of RTD at nucleotide and amino acid levels revealed a high identity (91.8–97.2% and 91.4–100% respectively) between Iranian and other available isolates in the GenBank. However, in regards to ORF1, a high genetic diversity among Iranian and other known PAV isolates at both amino acid (2–16.9%) and nucleotide (4.1–16.5%) levels were detected. Based on phylogenetic analysis of ORF1, two major groups of BYDV‐PAV isolates were distinguished. The Iranian isolates were divided between the two clusters. Our results suggest that the occurrence of two genetically distinct groups of PAV isolates in central and southern Iran, from which according to the ICTV criteria for species demarcation in the family Luteoviridae, four isolates from central parts of the country, qualify for designation as new species. 相似文献
8.
FRANÇOISE LECLERCQ-LE QUILLEC SYLVIE TANGUY C A DEDRYVER 《The Annals of applied biology》1995,126(1):75-90
During the years 1989–1992 cereal aphids were caught alive in a low level (1.5 m high) suction trap operated in Le Rheu (Brittany, France) and tested for BYDV transmission. In most cases comparisons with data collected simultaneously by a 12.2 m suction trap operating in the same site resulted in good relationships between weekly catches at both heights. Results from transmission tests showed that: (i) the two main BYDV vectors were Rhopalosiphum padi and Metopolophium dirhodum during the years of experiment; (ii) PAV and MAV were the commonest viruses and RPV was relatively scarce; (iii) during spring M. dirhodum appeared to be the most important MAV vector and nearly as good a PAV vector as R. padi; (iv) during autumn R. padi was the only vector of the three viruses with mixed transmission allowing it to transmit also MAV probably by heteroencapsidation. To give an indication of the risk of infection, infectivity indices were calculated by multiplying the numbers of aphids caught by the 12.2 m suction trap by the proportion that were infective. These infectivity indices agreed with field records of primary infections. 相似文献
9.
Weed and native grasses from the South Island of New Zealand were surveyed for virus infection. Cocksfoot mottle virus (CfMV)
and Ryegrass mosaic virus (RgMV) were restricted to a few introduced species; however, Barley yellow dwarf viruses (BYDVs)
have invaded native grasses in New Zealand. Virus incidence was significantly lower in the native species (2%) than in the
introduced species (12%). Four different serotypes (RMV, RPV, PAV, MAV) were detected in the introduced grass flora but only
two (RMV, PAV) were detected in native species. In experimental transmission tests the aphid vector Rhopalosiphum padi's survival was variable on the 20 native species tested but this was not due to the presence or absence of endophytic fungi
as none were detected in the New Zealand species. Aphid numbers increased and plants were killed when R. padi fed on Agrostis muelleriana and Festuca multinodis. R. padi transmitted a PAV isolate to these and six other native species. BYDVs infected 4/5 of the subfamilies tested. Virus incidence
in native Arundinoideae and Pooideae was significantly lower than in introduced Pooideae and Panicoideae. One species of Bambusoideae
collected from the field was not infected but was found susceptible in glasshouse tests. Agrostis capillaris, Dactylis glomerata and Lolium perenne were identified as the most likely reservoirs of infection for the native flora. Anthoxanthum odoratum was not infected but if the SGV serotype and its vector Schizaphis graminum were ever introduced, A. odoratum could form an effective reservoir from near sea level into alpine areas.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Purification and properties of two Swedish isolates of barley yellow dwarf virus and their serological relationships with American isolates 总被引:1,自引:0,他引:1
Two Swedish isolates of barley yellow dwarf virus (BYDV), one (39/78) transmitted much more efficiently by Rhopalosiphum padi than by Sitobion avenae and the other (27/77) transmitted specifically by Sitobion avenae, were purified with yields of 200–300 μg/100 g infected oat leaves. Three light scattering zones were obtained when isolate 39/78 was sedimented in 10–40% (w/w) sucrose density gradients but only one zone with the other isolate. The sedimentation coefficients of 39/78 particles were 94,106 and 150 S, respectively, whereas the 27/77 particles sedimented at 110 S. Four protein bands of mol. wts 31 900, 29 700, 28 000 and 15 000 were detected when disrupted 150 5 particles of the 39/78 isolate were electrophoresed in SDS-polyacrylamide gels. Only one major band of mol. wt 24 500 was detected when 94 or 106 S particles were electrophoresed under the same conditions. The 27/77 isolate yielded one major band of mol. wt 23 500. A weak serological relationship was found between isolates 39/78 and 27/77. In enzyme-linked immunosorbent assay, isolate 39/78 was serologically closely related to New York isolate PAY, whereas 27/77 was closely related to MAY. 相似文献
11.
Enzyme-linked immunosorbent assay (ELISA)-based surveys of the occurrence of five barley yellow dwarf virus (BYDV) serotypes (MAV, PAV and SGV in “Group 1”; RPV and RMV in “Group 2”) in CIMMYT bread wheat nurseries and other small grain crops in various locations world-wide were undertaken in 1988, 1989 and 1990. The objective was to investigate the relative occurrence of BYDV serotypes in areas relevant to CIMMYT cereal breeding programs. Overall, MAV and PAV serotypes predominated in the samples collected, though their relative frequencies depended on the location. SGV serotypes were uncommon in most locations. Group 2 serotypes occurred widely, but RMV serotypes were more common than RPV serotypes. 相似文献
12.
Rhopalosiphum padi and Sitobion avenae alates were collected from colonised winter cereals and maize in N.E. Spain and fed on young wheat plants for 7–10 days in the glasshouse. Then, aphids were killed and the plants on which aphids reproduced were kept in the glasshouse for 30–40 days. ELISA of infested plants was made using polyclonal and monoclonal antisera against PAV-, RPV- and MAV-like isolates. In autumn and spring, MAV serotypes were transmitted by S. avenae and R. padi , mainly in mixed infections with PAV serotypes. This possibly explains the high frequency of MAV-like isolates and their previously recorded year-to-year stability in maize, grain and forage winter cereals and cereal volunteers. PAV-like isolates were rarely transmitted by S. avenae and its spread thus depends almost exclusively on R. padi. These results confirm the importance of forage cereals and cereal volunteers as virus sources for winter cereal infection in the autumn, and the latter as a source of BYDV for maize in spring. 相似文献
13.
The effect of different virus isolates on the expression of tolerance to barley yellow dwarf virus in barley 总被引:1,自引:0,他引:1
A barley variety of Ethiopian origin, with a single Mendelian gene con-fering tolerance to barley yellow dwarf virus (BYDV), was equally tolerant to a number of isolates of the virus, whereas a susceptible European barley was more susceptible to isolates transmitted by Rhopalosiphum padi L. than to those transmitted by Macrosiphum (Sitobion) avenae (Fab). However, hybrids between these two varieties homozygous for the Ethiopian tolerance gene were more tolerant to ‘mild’ than to ‘severe’ isolates, irrespective of the vector specificity. The European variety was damaged more severely by all isolates when infected early than when infected late in its development, but the hybrids were damaged more severely by M. awraae-transmitted isolates when infected late. It is suggested that in susceptible plants the concentration, rather than the virulence, of the virus determines disease severity, whereas the reverse is true in plants possessing a gene which reduces virus multiplication. Virus concentration appears to determine the severity of R. padi-transmitted isolates, while virulence determines the severity of M. avenae-transmitted isolates. The latter would also seem to be adapted towards late infection. 相似文献
14.
SUSAN E. HALBERT B. JUNE CONNELLY G. W. BISHOP JACQUELYN L. BLACKMER 《The Annals of applied biology》1992,121(1):105-121
Populations of cereal aphids were sampled from 1985–1988 and assayed for transmission of barley yellow dwarf virus (BYDV), Rhopalosiphum padi, Rho-palosiphum maidis, Sitobion avenae, Metopolophium dirhodum, Schizaphis graminum and Macrosiphum euphorbiae collected from host plants transmitted BYDV in bioassays. Of the 1028 Diuraphis noxia collected from plants, one may have transmitted BYDV. The isolate involved resembled SGV in serological and biological characteristics, but since it was not recoverable by any of more than 800 D. noxia subsequently tested, we suspect it may have been a contaminant. Among those aphids collected during the autumn from a suction trap adapted for live collection, R. padi transmitted BYDV most frequently. Other trapped species which transmitted BYDV included: R. maidis, Rhopalosiphum insertum, Macrosiphum euphorbiae, Metopolophium dirhodum and Ceruraphis eriophori. An adapted Infectivity Index indicated that R. padi is by far the most important vector of BYDV during the autumn sowing season in southwestern Idaho. Male R. padi consistently transmitted BYDV more frequently than did females collected during the same period. 相似文献
15.
Barley yellow dwarf virus, wheat, and Sitobion avenae: a case of trilateral interactions 总被引:2,自引:0,他引:2
M. Fiebig H.-M. Poehling & C. Borgemeister 《Entomologia Experimentalis et Applicata》2004,110(1):11-21
We analysed interactions in the system of two Barley Yellow Dwarf Virus (BYDV) strains (MAV and PAV), and wheat (cv. Tinos) as host plant for the virus, and the cereal aphid Sitobion avenae (F.) as vector, in particular whether or not infection by the virus might alter host plant suitability in favour of vector development. By measuring the amino acid and sugar content in the phloem sap of infected and non‐infected wheat plants we found a significant reduction in the concentration of the total amount of amino acids on BYDV‐infected plants. Qualitative and quantitative analysis of honeydew and honeydew excretion indicated a lower efficiency of phloem sap utilisation by S. avenae on infected plants. In addition, S. avenae excreted less honeydew on infected plants. Both BYDV strains significantly affected aphid development by a reduction in the intrinsic rate of natural increase. Hence, infection by the virus reduced the host suitability in terms of aphid population growth potential on BYDV‐infected plants. However, more alate morphs developed on virus‐infected plants. These findings are discussed in relation to the population dynamics of S. avenae, and, as a consequence, the spread of BYDV. 相似文献
16.
R. T. PLUMB 《The Annals of applied biology》1976,83(1):53-59
Suction traps operating at low level (1 5 m) were used to catch live alate Rhopalosiphum padi, Macrosiphum (Sitobion) avenae and Metopolophium dirhodum which were tested for transmission of barley yellow dwarf virus (BYDV). The first species caught and infective was R. padi, followed by M. (S.) avenae infective some 2–3 wk later and M. dirhodum 3–4 wk later still. Never more than 11-5% of the annual catch of any species transmitted BYDV and the proportion fluctuated from week to week and between seasons in different years. The relative abundance of infective vectors of ths three species varied; annual numbers of infective M. (S.) avenae and M. dirhodum varied inversely with infective R. padi, the latter also usually transmitted severer virus. The results of the infectivity tests have been compared with the catches of these aphids by the Rothamsted Insect Survey and show that numbers of alate aphids do not necessarily indicate the likely incidence of BYDV. 相似文献
17.
Occurrence of barley yellow dwarf virus (BYDV) isolates in different farmland habitats in western France and south-west England 总被引:1,自引:0,他引:1
M HENRY S GEORGE G M ARNOLD C A DEDRYVER D A KENDALL Y ROBERT B D SMITH 《The Annals of applied biology》1993,123(2):315-329
The incidence and distribution of the three principal isolates of barley yellow dwarf virus (PAV, RPV and MAV) are described in winter cereal crops, cereal (stubble) regrowth and grasses from 11 sites in western France and south-west England during 1987 and 1988. Isolates were identified by indirect ‘sandwich’ ELISA using the monoclonal antibodies MAC91, MAC92 and MAFF2. More virus infection occurred in all localities and in most of the plant communities sampled, with the exception of perennial grass leys, in 1987 than in 1988. All three isolates were widespread. MAV was associated more with sites further north and PAV more with those further south. The geographical distribution of RPV was less variable. Underlying these trends, the relative abundance of isolates differed considerably between habitats. RPV always predominated in perennial grass leys and MAV in most cereal crops, although in the latter MAV was less prevalent in 1987 than in 1988. The greatest regional difference was found in stubble regrowth where PAV predominated in France but MAV predominated in England. Grasses from field margins (only sampled in England) were mainly infected by MAV and RPV. The implications of these findings for the epidemiology of BYDV are discussed, especially the roles of different host plant communities or habitats in the annual infection cycle of small-grain cereals. 相似文献
18.
E. Schliephake A. Habekuss M. Scholz F. Ordon 《Entomologia Experimentalis et Applicata》2013,146(3):347-356
A Hordeum bulbosum L. (Poaceae) clone A17 was identified, which showed complete resistance to Barley yellow dwarf virus (BYDV) and Cereal yellow dwarf virus (CYDV). It was not possible to infect plants of A17 with BYDV‐PAV, ‐MAV, or with CYDV‐RPV by the aphid vectors Rhopalosiphum padi (L.) or Sitobion avenae (Fabricius) (both Hemiptera: Aphididae). Plants of the A17 clone and of the BYDV‐susceptible H. bulbosum clone A21 revealed some resistance to R. padi compared to the susceptible winter barley cultivar Rubina [Hordeum vulgare L. (Poaceae)]. The development time to the imago was longer and the number of nymphs was reduced on both clones compared with cv. Rubina. The probing and feeding behaviour of R. padi on plants of the H. bulbosum clones was studied over 12 h and compared with that on plants of the barley cv. Rubina. Principal component analysis of the results of the feeding behaviour revealed a clear separation of the H. bulbosum genotypes from Rubina. On H. bulbosum the number of penetrations was higher but total feeding time was shorter. Significant differences were mainly found in the phloem feeding parameters for plants of both clones in comparison to Rubina, with the virus resistant A17 clone having the strongest effect and the susceptible A21 clone being intermediate. Most significant differences were found in parameters of the phloem salivation phase. On A17, an average of less than one (0.9) E1 phase per plant was observed (3.3 on A21 and 5.7 on Rubina) and its duration was reduced to less than 1 min (0.9 min) in comparison to 2.4 min on A21 and 5.7 min on Rubina. Also, the phloem feeding (E2) phase was clearly reduced on A17 plants with 0.5 E2 phases per test and a mean duration of 1.1 min in contrast with 2.9 and 3.5 E2 phases per test and 34.1 and 421.3 min for A21 and Rubina, respectively. These results point towards a phloem‐localized factor for aphid resistance in H. bulbosum, i.e., on A17 plants the phloem salivation time is too short for a successful infection by BYDV leading to vector resistance. 相似文献
19.
High Levels of Resistance in Agropyron Species to Barley Yellow Dwarf and Wheat Streak Mosaic Viruses 总被引:2,自引:0,他引:2
Several Agropyron species were tested for new sources of resistance to barley yellow dwarf virus (Bydv ) and wheat streak mosaic virus (WSMV). With BYDV strain PAV, 11 of the 17 Agropyron species showed no virus transmission when plants were given access feed by viruliferous Rhopalosiphum padi. Similar trials with BYDV strain RMV (vectored by R. maidis) indicated that all plants, except susceptible control plants, remained virus free. Virus status was confirmed by enzyme-linked immunosorbent assays. When plants were mechanically inoculated with WSMV, 11 Agropyron species failed to express symptoms, while five other species showed a segregating response or had some accessions segregating and some resistant. Test results suggest that resistance to BYDV and WSMV in Agropyron species does not appear to be correlated with any specific genome of Agropyron species although most of the Agropyron species containing S genome were resistant to BYDV and WSMV. 相似文献
20.
Vectoring ability of four aphid clones, Rp-M and Rp-R26 of Rhopalosiphum padi and Sa-R1 and Sa-V of Sitobion avenae, to transmit barley yellow dwarf (PAV, MAV and RPV) luteoviruses (BYDV) was compared in controlled conditions. Significant differences between highly efficient vectors (HEV), Rp-M and Sa-Rl, and poorly efficient vectors (PEV), Rp-R26 and Sa-V, were found in transmission of their specific viruses with acquisition and inoculation access periods (AAP, IAP) of 5 days. BYD-RPV was occasionally transmitted by both clones of S. avenae. None of 150 tested apterous adults of the Rp-R26 transmitted BYD-MAV, while 10% of transmission was observed from those of the Rp-M in a parallel test. An improved ELISA and immuno-PCR were adapted to test for viruses in aphids. The results obtained by the improved ELISA indicated there was a good correlation between virus detection in single aphids of HEV clones after a 5 day AAP and virus transmission by them. In contrast, the percentages of virus-carrying aphids of PEV clones were generally higher than those of their transmission rates. BYD-MAV and BYD-RPV were also detected by the improved ELISA in single aphids of their PEV clones, with the exception of BYD-RPV in those of Sa-V. However, after a 2-day IAP, the improved ELISA in most cases failed to detect these viruses in single aphids of PEV clones. Detection by immuno-PCR demonstrated that all three viruses could be acquired and retained by the aphids of both HEV and PEV clones. But, as visualised from electrophoretic bands, after the 2-day IAP the amplified products from aphid extracts of PEV clones were reduced. The detection in a batch of nine aphids by the improved ELISA revealed that virus content in PEV clones decreased more rapidly than that in HEV clones during transmission. Thus, the difference in transmission efficiency of the aphid clones within species was not caused by an inability to acquire virus, but was determined by variation in vectoring ability between them. This was due to differences in ability to prevent the passage of virions from haemocoel to salivary duct and/or different capacities for the retention of BYDV. 相似文献