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1.
Clive A. Slaughter 《Journal of biomolecular techniques》2013,24(4):232-237
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, Georgia Regents University–University of Georgia Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Phone: 706-713-2216; Fax: 706-713-2221; E-mail: ude.agu@thgualsc; or to any member of the editorial board. Article summaries reflect the reviewer''s opinions and not necessarily those of the association. 相似文献
2.
Clive A. Slaughter 《Journal of biomolecular techniques》2014,25(3):87-91
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, Georgia Regents University-University of Georgia Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Phone: 706-713-2216; Fax: 706-713-2221; E-mail: ude.agu@thgualsc; or to any member of the Editorial Board. Article summaries reflect the reviewer''s opinions and not necessarily those of the association. 相似文献
3.
Clive Slaughter 《Journal of biomolecular techniques》2010,21(1):54-60
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information about articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 279 William St., Athens, GA 30607-1777, USA. Tel.: (706) 369-5945: Fax: (706) 369-5936; E-mail: ude.gcm.liam@rethgualsc; or to any member of the editorial board. Article summaries reflect the reviewer''s opinions and not necessarily those of the association. 相似文献
4.
Clive A. Slaughter 《Journal of biomolecular techniques》2014,25(2):61-66
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, Georgia Regents University-University of Georgia Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Phone: 706-713-2216; Fax: 706-713-2221; E-mail: ude.agu@thgualsc; or to any member of the Editorial Board. Article summaries reflect the reviewer''s opinions and not necessarily those of the association. 相似文献
5.
Clive A. Slaughter 《Journal of biomolecular techniques》2014,25(1):41-47
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, Georgia Regents University/University of Georgia Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA (Phone: 706-713-2216; Fax: 706-713-2221; E-mail; ude.agu@thgualsc), or to any member of the editorial board. Article summaries reflect the reviewer''s opinions and not necessarily those of the association. 相似文献
6.
Clive A. Slaughter 《Journal of biomolecular techniques》2015,26(3):113-117
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, Georgia Regents University-University of Georgia Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Phone: 706-713-2216; Fax: 706-713-2221; E-mail: ude.agu@thgualsc; or to any member of the Editorial Board. Article summaries reflect the reviewer’s opinions and not necessarily those of the association. 相似文献
7.
Clive A. Slaughter 《Journal of biomolecular techniques》2015,26(2):74-78
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, Georgia Regents University-University of Georgia Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Phone: 706-713-2216; Fax: 706-713-2221; E-mail: ude.agu@thgualsc; or to any member of the Editorial Board. Article summaries reflect the reviewer’s opinions and not necessarily those of the association. 相似文献
8.
Clive A. Slaughter 《Journal of biomolecular techniques》2013,24(3):178-180
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, GRU-UGA Medical Partnership, 1425 Prince Ave., Athens, GA 30606; Phone: 706-713-2216; Fax: 706-713-2221; E-mail: ude.agu@thgualsc; or to any member of the editorial board. Article summaries reflect the reviewer''s opinions and not necessarily those of the association. 相似文献
9.
《Journal of biomolecular techniques》2021,32(3):214
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, AU-UGA Medical Partnership, 1425 Prince Avenue, Athens GA 30606. Phone: (706) 713-2216; Fax: (706) 713-2221; E-mail: ude.agu@thgualsc or to any member of the editorial board. Article summaries reflect the reviewer''s opinions and not necessarily those of the Association. 相似文献
10.
《Journal of biomolecular techniques》2021,32(1):42
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Ave., Athens GA 30606, USA. Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail; ude.agu@thgualsc, or to any member of the editorial board. Article summaries reflect the reviewer’s opinions and not necessarily those of the Association. 相似文献
11.
Clive Slaughter 《Journal of biomolecular techniques》2009,20(5):297-302
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information about articles they feel is important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 279 William St., Athens, GA 30607-1777, USA; Tel.: (706) 369-5945; Fax: (706) 369-5936; E-mail: cslaughter@mail.mcg.edu; or to any member of the editorial board. Article summaries reflect the reviewer’s opinions and not necessarily those of the association. 相似文献
12.
Clive A. Slaughter 《Journal of biomolecular techniques》2016,27(2):84-89
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail: cslaught@uga.edu, or to any member of the editorial board. Article summaries reflect the reviewer''s opinions and not necessarily those of the association. 相似文献
13.
Clive A. Slaughter 《Journal of biomolecular techniques》2016,27(1):40-45
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA; Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail: cslaught@uga.edu, or to any member of the editorial board. Article summaries reflect the reviewer’s opinions and not necessarily those of the association. 相似文献
14.
Clive A. Slaughter 《Journal of biomolecular techniques》2015,26(4):150-156
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to: Clive Slaughter, GRU-UGA Medical Partnership, 1425 Prince Ave., Athens, GA 30606, USA. Tel: (706) 713-2216; Fax: (706) 713-2221; E-mail: cslaught@uga.edu; or to any member of the editorial board. Article summaries reflect the reviewer’s opinions and not necessarily those of the association. 相似文献
15.
《Journal of biomolecular techniques》2021,32(2):83
This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, MCG-UGA Medical Partnership, 1425 Prince Avenue, Athens GA 30606. Tel; (706) 713-2216: Fax; (706) 713-2221: Email; cslaught@uga.edu or to any member of the editorial board. Article summaries reflect the reviewer’s opinions and not necessarily those of the Association. 相似文献
16.
Wild-type apocytochrome c and its hydrophobic segment deleted mutants, named 28–39, 72–86 and 28–29/72–86 were constructed, expressed and highly purified respectively. Insertion ability into phospholipid monolayer, inducing leakage of entrapped fluorescent dye fluorescein sulfonate (FS) from liposomes, and translocation across model membrane system showed that the wild-type apoprotein and 28–39 almost exhibited the same characteristics, while mutants with segment 72–86 deletion did not. Furthermore, CD spectra, intrinsic fluorescence emission spectra, and the accessibility of the protein to the fluorescence quenchers: KI, acrylamide and HB demonstrated that the segment 72–86 deletion has a significant effect on the conformational changes of apocytochrome c following its interaction with phospholipid. On the basis of these results it is postulated that the C-terminal hydrophobic segment 72–86 plays an important role in the translocation of apocytochrome c across membrane. 相似文献
17.
H. J. Tsai J. -P. Ewert 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1987,161(2):295-304
Summary Previous experiments have shown that during prey-catching behavior (orienting, snapping) in response to a worm-like moving stripe common toads.Bufo bufo (L.) exhibit a contrast-and direction-dependent edge preference. To a black (b) stripe moving against a white (w) background (b/w), they respond (R*) preferably toward the leading (l) rather the trailing (t) edge (R
l
*
> R
t
*
), thus displaying head preference. If the contrastdirection is reversed (w/b), the stripe's trailing edge is preferred (R
l
*
< R
t
*
), hence showing tail preference. In the present study, neuronal activities of retinal classes R2 and R3 and tectal classes T5(2) and T7 have been extracellularly recorded in response to leading and trailing edges of a 3 ° × 30 ° stripe simulating a worm and traversing the centers of their excitatory receptive fields (ERF) horizontally at a constant angular velocity in variable movement direction (temporo-nasal or naso-temporal).The behavioral contrast-direction dependent edge preferences are best resembled by the responses (R) of prey-selective class T5(2) neurons (Rl Rt=101 for b/w, 0.31 for w/b) and T7 neurons (RlRt=61 for b/w, 0.41 for w/b); the T7 responses may be dendritic spikes. This property can be traced back to off-responses dominated retinal class R3 neurons (RlRt=61 for b/w, 0.51 for w/b), but not to class R2 (RlRt =1.21 for b/w and 0.91 for w/b). The respective edge preference phenomena are independent of the direction of movement.When stimuli were moved against a stationary black-white structured background, the head preference to the black stripe and the tail preference to the white stripe were maintained in class R3, T5(2), and T7 neurons. If the stripe traversed the ERF together with the structured background in the same direction at the same velocity, the responses of tectal class T5(2) and T7 neurons were strongly inhibited, particularly in the former. Responses of retinal R2 neurons in comparable situations could be reduced by about 50%, while class R3 neurons responded to both the stimulus and the moving background structure.The results support the concept that the prey feature analyzing system in toads applies principles of (i) parallel and (ii) hierarchial information processing. These are (i) divergence of retinal R3 neuronal output contributes to stimulus edge positioning and (in combination with R2 output) area evaluation intectal neurons and to stimulus area evaluation and (in combination with R4 output) sensitivity for moving background structures inpre tectal neurons; (ii) convergence of tectal excitatory and pretectal inhibitory inputs specify the property of prey-selective tectal T5(2) neurons which are known to project to bulbar/spinal motor systems.Abbreviations
ERF
excitatory receptive field
-
IRF
inhibitory receptive field
-
N
nasal
-
T
temporal
-
R
w
response to a worm-like stripe moving in the direction of its longer axis
-
R
A
response to an antiworm-like stripe whose longer axis is oriented perpendicular to the direction of movement
-
R
l
response to the leading edge of a worm-like moving stripe
-
R
t
response to the trailing edge of a worm-like moving stripe
-
b/w
black stimulus against a white background
-
w/b
white stimulus against a black background
-
sm
structured moving background
-
ss
structured stationary background
-
u
minimal structure width of a structured background consisting of rectangular black and white patches in random distribution
-
HRP
horseradish peroxidase 相似文献
18.
SNAP-tag and CLIP-tag protein labeling systems enable the specific, covalent attachment of molecules, including fluorescent dyes, to a protein of interest in live cells. These systems offer a broad selection of fluorescent substrates optimized for a range of imaging instrumentation. Once cloned and expressed, the tagged protein can be used with a variety of substrates for numerous downstream applications without having to clone again. There are two steps to using this system: cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. The SNAP-tag is a small protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein. SNAP-tag labels are dyes conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. CLIP-tag is a modified version of SNAP-tag, engineered to react with benzylcytosine rather than benzylguanine derivatives. When used in conjunction with SNAP-tag, CLIP-tag enables the orthogonal and complementary labeling of two proteins simultaneously in the same cells.Download video file.(47M, mov) 相似文献
19.
Summary An endo--glucanase of C.cellulolyticum was purified by a procedure involving concanavalin A (Con A)-Sepharose chromatography and polyacrylamide gel electrophoresis (PAGE). The enzyme produced G1 and oligosaccharides from CMC. Chromatography on Procion Red HE3B-Agarose proved to be useful in the separation of cellobiase from cellobiose dehydrogenase.Abbreviations CMC
carboxymethyl cellulose
- CM-cellulase
carboxymethyl cellulase
- FP-cellulase
filter paper degrading cellulase
- G1
glucose
- G2
cellobiose
- G3
cellotriose
- G4
cellotetraose
- G5
cellopentaose
- G6
cellohexaose
- G7
celloheptaose
- -MG
methyl--D-glucoside
- pNPG
p-nitrophenyl--glucopyranoside
- pNP
p-nitrophenol
- CBDH
cellobiose dehydrogenase 相似文献
20.
Genetically-modified mice have emerged as a preferable animal model to study the molecular mechanisms underlying conduction abnormalities, atrial and ventricular arrhythmias, and sudden cardiac death.1 Intracardiac pacing studies can be performed in mice using a 1.1F octapolar catheter inserted into the jugular vein, and advanced into the right atrium and ventricle. Here, we illustrate the steps involved in performing programmed electrical stimulation in mice. Surface ECG and intracardiac electrograms are recorded simultaneously in the atria, atrioventricular junction, and ventricular myocardium, whereas intracardiac pacing of the atrium is performed using an external stimulator. Thus, programmed electrical stimulation in mice provides unique opportunities to explore molecular mechanisms underlying conduction defects and cardiac arrhythmias.Download video file.(84M, mp4) 相似文献