共查询到20条相似文献,搜索用时 15 毫秒
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Plant growth and productivity are adversely affected by various abiotic and biotic stress factors. In our previous study,
we used Prosopis juliflora, an abiotic stress tolerant tree species of Fabaceae, as a model plant system for isolating genes functioning in abiotic stress tolerance. Here we report the isolation and characterization
of a Rab family GTPase from P. juliflora (Pj Rab7) and the ability of this gene to confer salt stress tolerance in transgenic tobacco. Northern analysis for Pj Rab7 in P. juliflora leaf tissue revealed up-regulation of this gene under salt stress under the concentrations and time points analyzed. Pj Rab7 transgenic tobacco lines survived better under conditions of 150 mM NaCl stress compared to control un-transformed plants.
Pj Rab7 transgenic plants were found to accumulate more sodium than control plants during salt stress. The results of our studies
could be used as a starting point for generation of crop plants tolerant to abiotic stress. 相似文献
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Yu Cheng Wang Guan Zheng Qu Hong Yan Li Ying Jie Wu Chao Wang Gui Feng Liu Chuan Ping Yang 《Molecular biology reports》2010,37(2):1119-1124
Superoxide dismutases (SODs) play important role in stress tolerance of plants. In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana × P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC)
and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that
SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants
when exposed to NaCl stress. In addition, the relative weight gains of the transgenic plants were 8- to 23-fold of those observed
for WT plants after NaCl stress for 30 days. The data showed that the SOD activities that increased in transgenic lines are
1.3–4-folds of that increased in the WT plant when exposed to NaCl stress. Our analysis showed that increases in SOD activities
as low as 0.15-fold can also significantly enhance salt tolerance in transgenic plants, suggesting an important role of increased
SOD activity in plant salt tolerance. 相似文献
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The C2H2 zinc‐finger protein SlZF3 regulates AsA synthesis and salt tolerance by interacting with CSN5B
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Ying Li Zhuannan Chu Jinying Luo Yuhong Zhou Yujing Cai Yongen Lu Junhui Xia Hanhui Kuang Zhibiao Ye Bo Ouyang 《Plant biotechnology journal》2018,16(6):1201-1213
Abiotic stresses are a major cause of crop loss. Ascorbic acid (AsA) promotes stress tolerance by scavenging reactive oxygen species (ROS), which accumulate when plants experience abiotic stress. Although the biosynthesis and metabolism of AsA are well established, the genes that regulate these pathways remain largely unexplored. Here, we report on a novel regulatory gene from tomato (Solanum lycopersicum) named SlZF3 that encodes a Cys2/His2‐type zinc‐finger protein with an EAR repression domain. The expression of SlZF3 was rapidly induced by NaCl treatments. The overexpression of SlZF3 significantly increased the levels of AsA in tomato and Arabidopsis. Consequently, the AsA‐mediated ROS‐scavenging capacity of the SlZF3‐overexpressing plants was increased, which enhanced the salt tolerance of these plants. Protein–protein interaction assays demonstrated that SlZF3 directly binds CSN5B, a key component of the COP9 signalosome. This interaction inhibited the binding of CSN5B to VTC1, a GDP‐mannose pyrophosphorylase that contributes to AsA biosynthesis. We found that the EAR domain promoted the stability of SlZF3 but was not required for the interaction between SlZF3 and CSN5B. Our findings indicate that SlZF3 simultaneously promotes the accumulation of AsA and enhances plant salt‐stress tolerance. 相似文献
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Li-Wen Jing Shi-Hua Chen Xiao-Li Guo Hui Zhang Yan-Xiu Zhao 《植物学报(英文版)》2006,48(10):1244-1249
Ablotlc stress, such as salt, drought and extreme temperature, can result in enhanced production of reactive oxygen species (ROS). Plants have developed both enzymatic ROS-scavenging and non-enzymatic ROS-scavenging systems. The major ROS-scavenging enzymes of plants include superoxide dismutase (SOD), ascorbate peroxldaae (APX), catalaae (CAT), glutathione peroxldaae (GPX) and peroxiredoxina (Prxa). In the present work, we identified a gene encoding chloroplast-located peroxiredoxin Q, SsPrxQ, from Suaeda salsa L. located at chloroplast. Overexpression of SsPrxQ In Arabidopsis leads to an increase In salt and low-temperature tolerance. 相似文献
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Salt tolerance in crop plants: new approaches through tissue culture and gene regulation 总被引:10,自引:0,他引:10
Recent approaches to study of salinity tolerance in crop plants have ranged from genetic mapping to molecular characterization
of gene products induced by salt/drought stress. Transgenic plant design has allowed to test the effects of overexpression
of specific prokaryotic or plant genes that are known to be up-regulated by salt/drought stress. This review summarizes current
progress in the field in the context of adaptive metabolic and physiological responses to salt stress and their potential
role in long term tolerance. Specifically considered are gene activation by salt, in view of proposed avenues for improved
salt tolerance and the need to ascertain the additional influences of developmental regulation of such genes. Discussion includes
the alternate genetic strategy we have pursued for improving salinity tolerance in alfalfa (Medicago sativa L.) and rice (Oryza sativa L.). This strategy combines single-step selection of salt-tolerant cells in culture, followed by regeneration of salt-tolerant
plants and identification of genes important in conferring salt tolerance. We have postulated that activation or improved
expression of a subset of genes encoding functions that are particularly vulnerable under conditions of salt-stress could
counteract the molecular effects of such stress and could provide incremental improvements in tolerance. We have proceeded
to identify the acquired specific changes in gene regulation for our salt-tolerant mutant cells and plants. One particularly
interesting and novel gene isolate from the salt-tolerant cells is Alfin1, which encodes a putative zinc-finger regulatory protein, expressed predominantly in roots. We have demonstrated that this
protein binds DNA in a sequence specific manner and may be potentially important in gene regulation in roots in response to
salt and an important marker for salt tolerance in crop plants. 相似文献
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Jianhua Zhu Byeong‐Ha Lee Mike Dellinger Xinping Cui Changqing Zhang Shang Wu Eugene A. Nothnagel Jian‐Kang Zhu 《The Plant journal : for cell and molecular biology》2010,63(1):128-140
Osmotic stress imposed by soil salinity and drought stress significantly affects plant growth and development, but osmotic stress sensing and tolerance mechanisms are not well understood. Forward genetic screens using a root‐bending assay have previously identified salt overly sensitive (sos) mutants of Arabidopsis that fall into five loci, SOS1 to SOS5. These loci are required for the regulation of ion homeostasis or cell expansion under salt stress, but do not play a major role in plant tolerance to the osmotic stress component of soil salinity or drought. Here we report an additional sos mutant, sos6‐1, which defines a locus essential for osmotic stress tolerance. sos6‐1 plants are hypersensitive to salt stress and osmotic stress imposed by mannitol or polyethylene glycol in culture media or by water deficit in the soil. SOS6 encodes a cellulose synthase‐like protein, AtCSLD5. Only modest differences in cell wall chemical composition could be detected, but we found that sos6‐1 mutant plants accumulate high levels of reactive oxygen species (ROS) under osmotic stress and are hypersensitive to the oxidative stress reagent methyl viologen. The results suggest that SOS6/AtCSLD5 is not required for normal plant growth and development but has a critical role in osmotic stress tolerance and this function likely involves its regulation of ROS under stress. 相似文献
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Dong Cao Wensheng Hou Wei Liu Weiwei Yao Cunxiang Wu Xiaobing Liu Tianfu Han 《Plant Cell, Tissue and Organ Culture》2011,107(3):541-552
Salinity is a major factor resulting in extensive loss of agricultural production. Genetic transformation has become a powerful
tool for studying gene function and for improving crop salt tolerance. In this study, a TaNHX2 gene was transformed into a plant cloning vector under the control of cauliflower mosaic virus 35S promoter, and then introduced
into Agrobacterium rhizogenes strain K599. Explants of soybean were transformed with A. rhizogenes and ‘composite’ plants consisting of wild-type shoots and transgenic hairy roots overexpressing TaNHX2 were produced. When exposed to salt stress, ‘composite’ plants displayed high salinity tolerance at 171 mM NaCl in vermiculite
and in solid medium supplemented with up to 200 mM NaCl, whereas control plants displayed chlorosis and died within 15 days
under above treatment conditions. We subsequently obtained soybean plants overexpressing TaNHX2 through A. tumefaciens-mediated transformation and studied four homozygous lines of TaNHX2. Transgenic lines displayed an enhanced salt tolerance in plant biomass and flower number per plant, compared with wild type
plants grown on sand culture containing 150 mM NaCl. Furthermore, transgenic plants of line C12-11 showed longer survival,
less growth inhibition and greater number of flowers than wild type plants. Taken together, these results indicated that TaNHX2 gene could enhance salt tolerance of soybean, and A. rhizogenes-mediated transformation system could be used as a complementary tool of A. tumerfaciens-mediated transformation to rapidly investigate candidate gene function in soybean. 相似文献
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Degao Liu Lianjun Wang Chenglong Liu Xuejin Song Shaozhen He Hong Zhai Qingchang Liu 《PloS one》2014,9(4)
Iron-sulfur cluster biosynthesis involving the nitrogen fixation (Nif) proteins has been proposed as a general mechanism acting in various organisms. NifU-like protein may play an important role in protecting plants against abiotic and biotic stresses. An iron-sulfur cluster scaffold protein gene, IbNFU1, was isolated from a salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line LM79 in our previous study, but its role in sweetpotato stress tolerance was not investigated. In the present study, the IbNFU1 gene was introduced into a salt-sensitive sweetpotato cv. Lizixiang to characterize its function in salt tolerance. The IbNFU1-overexpressing sweetpotato plants exhibited significantly higher salt tolerance compared with the wild-type. Proline and reduced ascorbate content were significantly increased, whereas malonaldehyde (MDA) content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbNFU1 up-regulated pyrroline-5-carboxylate synthase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS) scavenging genes was found in the transgenic plants under salt stress. These findings suggest that IbNFU1gene is involved in sweetpotato salt tolerance and enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system. 相似文献
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Overexpression of ethylene response factor <Emphasis Type="Italic">TERF2</Emphasis> confers cold tolerance in rice seedlings 总被引:1,自引:0,他引:1
Rice (Oryza sativa L.) is a warm-season plant exposed to various stresses. Low temperature is an important factor limiting extension of rice
cultivation areas and productivity. Previously, we have demonstrated that tomato ERF protein TERF2 enhances freezing tolerance
of transgenic tobacco and tomato plants. Herein, we report that overexpression of TERF2 enhances transgenic rice tolerance to cold without affecting growth or agronomic traits. Physiological assays revealed that
TERF2 could not only increase accumulation of osmotic substances and chlorophyll, but also reduce reactive oxygen species
(ROS) and malondialdehyde (MDA) content and decrease electrolyte leakage in rice under cold stress. Further analysis of gene
expression showed that TERF2 could activate expression of cold-related genes, including OsMyb, OsICE1, OsCDPK7, OsSODB, OsFer1, OsTrx23, and OsLti6, in transgenic rice plants under natural condition or cold stress. Thus, our findings demonstrated that TERF2 modulated expression
of stress-related genes and a series of physiological adjustments under cold stress, indicating that TERF2 might have important
regulatory roles in response to abiotic stress in rice and possess potential utility in improving crop cold tolerance. 相似文献
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Chenxi Xu Lei Zheng Caiqiu Gao Chao Wang Guifeng Liu Jing Jiang Yucheng Wang 《Plant Molecular Biology Reporter》2011,29(2):424-430
H+-ATPase subunit c (VHA-c) is involved in the adaptation to environmental stresses, including salt, drought, and heavy metals.
However, it remains unclear whether VHA-c can induce a physiological response related to stress tolerance. To investigate
this possibility, we generated transgenic tobacco lines overexpressing a V-ATPase subunit c (LbVHA-c1) gene from Limonium bicolor (Bunge) Kuntze. Compared with wild-type (WT) tobacco, superoxide dismutase (SOD) and peroxidase (POD) activities in the transgenic
plants were significantly enhanced under salt stress conditions. The level of malondialdehyde (MDA) in the transgenic plants
was significantly lower than that in WT plants grown under salt stress conditions. Moreover, the transgenic plants displayed
obviously better growth than the WT plants under salt stress. These results suggest that LbVHA-c1 may confer stress tolerance through enhancing POD and SOD activities, and by protecting membranes from damage by decreasing
lipid peroxidation under salt stress. 相似文献