共查询到20条相似文献,搜索用时 15 毫秒
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Cheryl Frankfater Erika Maus Krisztina Gaal Fernando Segade Neal G. Copeland Debra J. Gilbert Nancy A. Jenkins J. Michael Shipley 《Mammalian genome》2000,11(3):191-195
A 1.4-kb EST clone encoding mouse microfibril-associated glycoprotein-2 (MAGP-2), identified by its similarity with the reported
human cDNA, was used to screen a mouse 129 genomic bacterial artificial chromosome (BAC) library. The mouse gene contains
10 exons spanning 16 kb, located on the distal region of Chromosome (Chr) 6. The exons range in size from 24 to 963 bp, with
the ATG located in exon 2. The tenth and largest exon contains 817 bp of 3′ untranslated sequence, including a B2 repetitive
element. Northern analysis demonstrates abundant expression of MAGP-2 mRNA in skeletal muscle, lung, and heart. Sequence analysis
of additional cDNA clones suggests that the two mRNA forms of MAGP-2 in the mouse arise from alternative polyadenylation site
usage. The promoter does not contain an obvious TATA box, and the sequence surrounding the start site does not conform to
the consensus for an initiator promoter element. Additionally, the mouse promoter contains 22 copies of a CT dinucleotide
repeat sequence located ∼155 bp 5′ to exon 1.
Received: 27 August 1999 / Accepted: 2 November 1999 相似文献
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Dirks RP Potter SJ Griep AE 《Biochemical and biophysical research communications》2001,287(4):900-909
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Reinders J Rozemuller EH van Gent R Arts-Hilkes YH van den Tweel JG Tilanus MG 《Immunogenetics》2005,57(10):790-794
Most of the 119 human leukocyte antigen (HLA)-DPB1 alleles are defined by polymorphism in six hypervariable regions (HVRs)
in exon 2 of the HLA-DPB1 gene. We investigated how DPB1 polymorphism is represented in the entire coding region. An RNA sequencing-based
typing (SBT) approach was developed for the identification of HLA-DPB1 polymorphism from the 5′ untranslated region (UTR)
through the 3′-UTR. B-cell lymphoblastoid cell lines, encoding 16 different DPB1 alleles, were studied. Results show additional
HLA-DPB1 polymorphism in exons 1, 3, 4 and 5 and the 5′ and 3′-UTR. Four new HLA-DPB1 alleles were identified, DPB1*0502, DPB1*0602, DPB1*0802 and DPB1*0902, which have exon 2 sequences identical to other DPB1 alleles but differ in the extended region. The additional polymorphism
represents two main polymorphic lineages in the DPB1 alleles. Among the HVRs in exon 2, only HVR F correlates with these two
main lineages. 相似文献
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