阿尔泰蝠蛾(鳞翅目:蝙蝠蛾科)幼虫低温胁迫转录组分析

[目的]本研究旨在通过转录组测序技术分析低温胁迫引起的阿尔泰蝠蛾Hepialus altaicola Wang幼虫基因转录差异及上调表达基因的主要功能类群和参与的主要代谢通路.[方法]采用Illumina HiSeq TM 2500测序平台对阿尔泰蝠蛾幼虫进行转录组测序、组装,利用Blast软件进行数据库比对和基因功能注释,用DEGSeq R软件包分析4℃低温处理与室内适温饲养试虫的差异表达基因,并对上调表达基因进行GO和KEGG代谢途径富集分析.[结果]经序列拼接后共获得100300个unigenes,总长度81600309 bp,平均长度813 bp,N50长度1719 bp.与7大数据库同源比对,共获得34691(34.59％)条unigenes.低温胁迫转录组分析得到11569个差异表达基因(DEGs),7158条基因上调,4411条基因下调.富集到47个GO类群,217个KEGG途径.其中代谢过程、催化、结合活性类群占有重要比例,核糖体、碳代谢、剪接体等途径显著富集.另外,热激蛋白、昆虫表皮蛋白、海藻糖酶、超氧化物歧化酶等非生物胁迫相关基因显著上调表达.[结论]阿尔泰蝠蛾幼虫低温胁迫转录组分析揭示,代谢过程、细胞过程、生物调节、对刺激的反应等生物学过程相关基因和部分非生物胁迫响应基因显著上调表达,提示蝠蛾幼虫可能从抗氧化防御、分子伴侣、体温调节和维持细胞的渗透平衡等多方面应对低温胁迫.     

阿尔泰蝠蛾(鳞翅目:蝙蝠蛾科)幼虫低温胁迫转录组分析

[目的]本研究旨在通过转录组测序技术分析低温胁迫引起的阿尔泰蝠蛾Hepialus altaicola Wang幼虫基因转录差异及上调表达基因的主要功能类群和参与的主要代谢通路.[方法]采用Illumina HiSeq TM 2500测序平台对阿尔泰蝠蛾幼虫进行转录组测序、组装,利用Blast软件进行数据库比对和基因功能注释,用DEGSeq R软件包分析4℃低温处理与室内适温饲养试虫的差异表达基因,并对上调表达基因进行GO和KEGG代谢途径富集分析.[结果]经序列拼接后共获得100300个unigenes,总长度81600309 bp,平均长度813 bp,N50长度1719 bp.与7大数据库同源比对,共获得34691(34.59％)条unigenes.低温胁迫转录组分析得到11569个差异表达基因(DEGs),7158条基因上调,4411条基因下调.富集到47个GO类群,217个KEGG途径.其中代谢过程、催化、结合活性类群占有重要比例,核糖体、碳代谢、剪接体等途径显著富集.另外,热激蛋白、昆虫表皮蛋白、海藻糖酶、超氧化物歧化酶等非生物胁迫相关基因显著上调表达.[结论]阿尔泰蝠蛾幼虫低温胁迫转录组分析揭示,代谢过程、细胞过程、生物调节、对刺激的反应等生物学过程相关基因和部分非生物胁迫响应基因显著上调表达,提示蝠蛾幼虫可能从抗氧化防御、分子伴侣、体温调节和维持细胞的渗透平衡等多方面应对低温胁迫.     

冰菜盐胁迫下的转录组分析

为了解冰菜(Mesembryanthemum crystallinum)叶片抗盐相关基因组学,利用Illumina Hi-seq TM2500高通量测序技术研究冰菜叶片在400 mmol L~(–1) NaCl胁迫下转录组基因的差异表达。结果表明,从400 mmol L~(–1) NaCl胁迫和对照的冰菜叶片中共获得13.01 Gb Clean data,Q30碱基均大于90.08%。共获得123个差异表达基因(DEGs),包括73个上调基因,50个下调基因,其中功能注释的基因有96个。根据Unigene库序列进行GO、COG和KEGG注释,筛选出8个与抗盐性相关差异表达基因,植物激素代谢相关基因,脱落酸8'-羟基化酶、吲哚-3-乙酰酸酰胺合成酶和茉莉酮酸酯ZIM结构域蛋白基因均下调表达,生长素响应蛋白、细胞分裂素合酶基因则上调表达,糖代谢相关基因棉子糖合成酶基因上调表达,质膜H+-ATPase基因上调表达,脱水蛋白基因下调表达。这为冰菜耐盐基因组学和分子生物学的研究奠定基础。     

基于比较转录组学分析NaCl 胁迫影响德尔卑沙门氏菌的耐渗机制

【目的】研究高渗胁迫条件下德尔卑沙门氏菌(Salmonella enterica subsp.enterica Derby,S.Derby)的转录组调控机制,分析差异表达基因(differentially expressed genes,DEGs)表达水平,探究在高渗胁迫影响下德尔卑沙门氏菌耐渗反应的相关代谢通路。【方法】通过高渗胁迫诱导德尔卑沙门氏菌的耐渗性,提取菌株的总RNA,去除rRNA,构建cDNA文库。利用转录组测序技术及生物学信息技术分析相关DEGs,并通过实时荧光定量PCR(real-time fluorescence quantitative PCR,qRT-PCR)进行验证。【结果】胁迫组德尔卑沙门氏菌通过转录组测序结果发现有3950个DEGs,其中具有显著上调的基因21个,显著下调基因38个。涉及到细胞膜蛋白、氨基酸的代谢等相关基因上调,协助德尔卑沙门氏菌在高渗环境中存活。与此同时,胁迫组德尔卑沙门氏菌的糖转运系统(sugar transport system,PTS)、糖酵解过程以及抗氧化性相关基因表达显著下调,这是由于高渗环境菌体需要在体内储存大量糖类等物质,从而降低了糖原的消耗,进而导致细胞外膜的脂多糖合成受到抑制,降低了高渗胁迫下德尔卑沙门氏菌细胞膜表面的O抗原的合成。【结论】高渗环境诱导后显著提高了德尔卑沙门氏菌的耐渗性,其中Na+/H+逆向转运蛋以及谷氨酸的代谢通路发挥着重要的作用,为进一步了解以及更好地控制其在食品中的污染提供了理论依据。     

短期盐胁迫下盐穗木的转录组分析

盐穗木（Halostachys caspica）是荒漠盐碱地广泛分布的盐生植物,具有极强的耐盐性。为揭示盐胁迫下盐穗木基因组层面的基因表达变化特性,通过对300和500mmol·L^-1 NaCl胁迫3h的盐穗木同化枝进行了转录组测序。有效序列组装共得到153298条平均长度为643bp的unigenes,进行GO和KEGG功能聚类,分别获得47个GO功能小类和118个KEGG通路。差异表达基因分析显示,短期低盐（300mmol·L^-1）响应基因有4432个,高盐（500mmol·L^-1）响应基因有2580个,两个胁迫的共差异基因有1245个,主要富集在细胞过程、代谢过程和响应刺激等类别中。从短期盐胁迫下盐穗木转录组筛选出渗透调节和活性氧清除的相关基因,大多为上调基因。说明盐穗木能够通过促进渗透调节和增强活性氧清除提高短期的盐胁迫适应能力。     

东乡野生稻苗期响应低温胁迫的转录组分析

为了解东乡野生稻（Oryza rufipogon）对低温胁迫的响应机制,对苗期的RNA-seq转录表达谱进行了研究。结果表明,与对照相比,共检测到10 200个差异表达基因（DEGs）,其中5 201个上调表达,4 999个下调表达,其中有426个DEGs位于已报道的水稻耐冷QTL区间,且37个为耐冷调控相关的家族基因。GO功能分类和KEGG代谢路径分析表明,核酸结合转录因子活性、氨基酸生物合成以及光合作用代谢等均参与响应低温胁迫过程。实时荧光定量分析表明,ABA响应蛋白基因、MYB转录因子和40S核糖体蛋白SA基因等12个可能与低温胁迫响应相关的DEGs表达模式与RNA-seq的一致。可见,植物激素传导途径和转录因子相关调控基因在东乡野生稻苗期响应低温胁迫过程中起重要作用。     

盐度胁迫对大黄鱼能量代谢与线粒体自噬的影响

为了比较高盐和低盐胁迫对大黄鱼(Larimichthys crocea)肝脏能量代谢和线粒体自噬的影响差异及其作用机制,将体质量为(53.46±1.47) g大黄鱼放入盐度为12、25或40的水体暴露1d、3d和7d,取其肝脏样本检测氧化损伤、能量代谢和线粒体自噬相关指标。结果显示,胁迫1d时,高盐组的大黄鱼肝脏三羧酸循环酶活力和线粒体自噬基因表达水平显著高于低盐组,表明鱼类在盐度胁迫初期需要消耗更多的能量,并提高线粒体自噬来应对高盐胁迫。胁迫7d时,高盐组的大黄鱼肝脏ATP合成酶活力和微管相关蛋白轻链3基因表达水平低于低盐组,活性氧簇和乳酸含量,乙酰辅酶A羧化酶活力高于低盐组,表明高盐组的大黄鱼在胁迫末期降低了有氧代谢和线粒体自噬,提高了无氧代谢,导致机体能量供应不足,线粒体自噬受到抑制,从而加重了鱼类氧化损伤。在盐度胁迫过程中,腺苷酸活化蛋白激酶和叉头框转录因子O亚型3分别在调控能量代谢酶活力和线粒体自噬基因表达方面发挥重要作用。研究结果揭示了高盐和低盐胁迫对大黄鱼能量代谢与线粒体自噬的影响差异及其初步机制,可为大黄鱼养殖水体的盐度调节方案制定及养殖水域选择提供基础资料。     

基于比较转录组学分析NaCl胁迫影响德尔卑沙门氏菌的耐渗机制

【目的】研究高渗胁迫条件下德尔卑沙门氏菌(Salmonella enterica subsp. enterica Derby, S. Derby)的转录组调控机制,分析差异表达基因(differentially expressed genes, DEGs)表达水平,探究在高渗胁迫影响下德尔卑沙门氏菌耐渗反应的相关代谢通路。【方法】通过高渗胁迫诱导德尔卑沙门氏菌的耐渗性,提取菌株的总RNA,去除rRNA,构建cDNA文库。利用转录组测序技术及生物学信息技术分析相关DEGs,并通过实时荧光定量PCR (real-time fluorescence quantitative PCR, qRT-PCR)进行验证。【结果】胁迫组德尔卑沙门氏菌通过转录组测序结果发现有3 950个DEGs,其中具有显著上调的基因21个,显著下调基因38个。涉及到细胞膜蛋白、氨基酸的代谢等相关基因上调,协助德尔卑沙门氏菌在高渗环境中存活。与此同时,胁迫组德尔卑沙门氏菌的糖转运系统(sugar transport system, PTS)、糖酵解过程以及抗氧化性相关基因表达显著下调,这是由于高渗环境菌体需要在体内储存大量糖类等物质,从而降低了糖原的消耗,进而导致细胞外膜的脂多糖合成受到抑制,降低了高渗胁迫下德尔卑沙门氏菌细胞膜表面的O抗原的合成。【结论】高渗环境诱导后显著提高了德尔卑沙门氏菌的耐渗性,其中Na⁺/H⁺逆向转运蛋以及谷氨酸的代谢通路发挥着重要的作用,为进一步了解以及更好地控制其在食品中的污染提供了理论依据。     

盐胁迫对橙色莫桑比克罗非鱼AQP1基因表达的影响

水通道蛋白(aquaporin,AQP)是一类细胞膜通道蛋白,能够选择性地高效转运水分子。为研究橙色莫桑比克罗非鱼(Oreochromis mossambicus)AQP1基因在渗透压调控中的作用,该实验克隆了橙色莫桑比克罗非鱼的AQP1基因,并利用实时荧光定量方法(q PCR)分析了该基因在各个组织中的表达分布及其在盐度梯度胁迫(低盐胁迫(22‰)和高盐胁迫(35‰))条件下鳃、肾和肌肉的表达特征。结果显示AQP1基因c DNA全长2 612 bp,开放阅读框(ORF)774 bp,编码258个氨基酸;其DNA序列全长3 215 bp,包含2个内含子,3个外显子。组织分布结果表明,AQP1基因在各组织都有表达,在肾、皮肤和肌肉中表达量相对较高;盐胁迫结果显示,在盐度为22时鳃和肾中表达量在6 h达到峰值,肌肉中在24 h达到峰值;当盐度升至35时,鳃、肾和肌肉表达量均升高。实验结果表明,AQP1基因的表达与盐度密切相关,并参与橙色莫桑比克罗非鱼的渗透压调控。     

白化菠萝蜜茎2个蛋白激酶和4个转录因子家族分析

为探究蛋白激酶(PKs)和转录因子(TFs)在白化菠萝蜜(Artocarpus heterophyllus)幼苗茎次生生长中的表达变化,基于转录组数据对其差异表达基因(DEGs)进行预测及分类,并对挑选出的2个PKs和4个TFs家族构建系统进化树。结果表明,胞质类受体激酶(RLCK)-VIII家族的DEGs上下调表达各4个,亮氨酸富集重复类受体激酶(LRR-RLK)-X家族Xa和Xb-2分支中的DEGs均下调表达,Xb-1中的均上调,TCP家族的20个DEGs中有15个上调表达,zf-HD和GRF家族中的大多数DEGs上调表达,Alfin-like家族中的DEGs均下调表达。因此,这表明6个家族可能在菠萝蜜茎的次生生长过程和应对非生物胁迫中发挥重要作用。     

Seed germination traits of two plant functional groups in the saline deltaic ecosystems

Aims Salt stress resulting from soil salinization is one of the driving forces of the land degradation throughout the world. The modern Yellow River delta is one of the most saline areas in China. Phytoremediation can be an effective way to restore the salinized ecosystems, which requires selecting appropriate plant species. This study explored the germination responses of common plant species from contrasting habitats in the Yellow River delta to varying salinity, offering experimental information for ecosystem restoration in the Yellow River delta.Methods In this study, 15 common plant species from the Yellow River delta were divided into two groups (high-salinity and low-salinity groups) by their natural habitats using Canonical Correlation Analysis. Seeds of each species were treated with five salinity levels (0, 5, 10, 20 and 30 ppt), using a randomized complete block design, and germinated seeds were counted and removed daily for 28 days to calculate the final germination proportion and mean time to germination. The germination responses of seeds to salinity treatments were compared between the two groups.Important findings In relation to salinity, seed germination behavior of the test species was closely related to the salinity level of the habitats over which they were distributed. Species from the habitats with higher salinity had generally higher final germination proportion but shorter mean time to germination than those from the habitats with lower salinity in all of five salinity treatments used. The final germination proportion and mean time to germination of low-salinity group species were more sensitive to salinity than those of high-salinity group species. Selecting the species with high final germination proportion and short mean time to germination is important for restoration of salinized land.     

Effects of developmental acclimation on adult salinity tolerance in the freshwater-invading copepod Eurytemora affinis

Invasive species are commonly thought to have broad tolerances that enable them to colonize new habitats, but this assumption has rarely been tested. In particular, the relative importance of acclimation (plasticity) and adaptation for invasion success are poorly understood. This study examined effects of short-term and developmental acclimation on adult salinity tolerance in the copepod Eurytemora affinis. This microcrustacean occurs in estuarine and salt marsh habitats but has invaded freshwater habitats within the past century. Effects of short-term acclimation were determined by comparing adult survival in response to acute versus gradual salinity change to low salinity (fresh water). Effects of developmental acclimation on adult tolerance were determined using a split-brood 4 x 2 factorial experimental design for one brackish-water population from Edgartown Great Pond, Massachusetts. Twenty full-sib clutches were split and reared at four salinities (fresh, 5, 10, and 27 practical salinity units [PSU]). On reaching adulthood, clutches from three of the salinity treatments (no survivors at fresh) were split into low- (fresh) and high- (40 PSU) salinity stress treatments, at which survival was measured for 24 h. Short-term acclimation of adults did not appear to have a long-term affect on low-salinity tolerance, given that gradual transfers to fresh water enhanced survival relative to acute transfers in the short term (after 7 h) but not over a longer period of 8 d. Developmental acclimation had contrasting effects on low- versus high-salinity tolerance. Namely, rearing salinity had a significant effect on tolerance of high-salinity (40 PSU) stress but no significant effect on tolerance of low-salinity (freshwater) stress. In addition, there was a significant effect of clutch on survival under freshwater conditions, indicating a genetic component to low-salinity tolerance but no significant clutch effect in response to high salinity. While developmental acclimation might enhance survival at higher salinities, the minimal effect of acclimation and significant effect of clutch on low-salinity tolerance suggest the importance of natural selection during freshwater invasion events.     

Innate immunity of the white shrimp Litopenaeus vannamei weakened by the combination of a Vibrio alginolyticus injection and low-salinity stress

White shrimp Litopenaeus vannamei were reared at a salinity of 35‰ without a Vibrio alginolyticus injection (unchallenged group), and other shrimp were reared at 35‰, injected with tryptic-soy broth (TSB)-grown V. alginolyticus at 1.8 × 10⁵ colony-forming units (cfu) shrimp^?1 (challenged group), and then examined for the hyaline cell (HC) count, granular cell (GC, including semi-granular cell) count, total haemocyte count (THC), phenoloxidase (PO) activity, respiratory burst (RB) and superoxide dismutase (SOD) activity after transfer to 35‰ (control), 25‰, 20‰, and 15‰ for 1, 6, 12, 24, 72, and 120 h. Results indicated that the haemocyte count, PO activity, RB, and SOD activity of unchallenged shrimp and challenged shrimp that were transferred to low-salinity levels all began to significantly decrease at 6, 6, 6, and 1 h, respectively, and reached the lowest levels at 12 h. HC, GC, the THC, PO activity, RB, and SOD activity of unchallenged shrimp that were transferred to 15‰ decreased by 53%, 41%, 49%, 68%, 39%, and 62%, whereas those parameters of challenged shrimp that were transferred to 15‰ decreased by 79%, 78%, 79%, 82%, 54%, and 72%, respectively after 12 h compared to control shrimp. These immune parameters began to recover after 24–72 h for both unchallenged shrimp and challenged shrimp. We concluded that the innate immunity was weakened in white shrimp L. vannamei that received combined stresses of a V. alginolyticus injection, and low-salinity transfer. It was also concluded that shrimp with respectively 21%, 18%, 46%, and 28% lower THC, PO activity, RB, and SOD activity of the original values would be killed due to decreases in their immunity, and resistance to V. alginolyticus infection. Shrimp farming should be maintained at a constant high salinity level to prevent exacerbated decreases in innate immune parameters of shrimp when infected by a pathogen coupled with low-salinity stress leading to mortality.     

Low-salinity stress in the American lobster,Homarus americanus,after chronic sublethal exposure to cadmium: Biochemical effects

Lobsters(Homarus americanus) were exposed to cadmium (6μg l⁻¹, 30 days) in flowing seawater, then held for 7 days in aerated “clean ” seawater at either ambient (27 ‰) or low (17 ‰) salinity. Cadmium exposure alone (ambient salinity) induced a general elevation of enzyme activity (heart, antennal gland, and muscle MDH; heart LDH and GPI), despite the probability of some clearance of cadmium from body tissues during the “clean ” seawater holding period. Low-salinity alone (non-exposed lobsters) caused a decrease of enzyme activity (AAT, LDH, GPI, PK) in most tissues examined, except for tail muscle IDH, the activity of which was increased, and MDH, which was significantly elevated above ambient controls in all tissues except heart. Most low-salinity effects were observed in tail muscle, and most cadmium effects, in heart; low-salinity effects outnumbered cadmium stress by nine to four. In heart and tail muscle of cadmium-exposed lobsters held at low salinity, each of the two stresses apparently operated to nullify the other's effects. The most prominent single biochemical response to these sublethal stresses was the elevation of MDH activity. The ratio MDH: LDH gave the clearest indication of overall relative stress.     

Effect of Salinity on Mercury-Methylating Activity of Sulfate-Reducing Bacteria in Estuarine Sediments

The biomethylation of mercury was measured in anoxic estuarine sediments that ranged in salinity from 0.03 to 2.4% with or without added molybdate, an inhibitor of sulfate reducers. Mercury methylation was inhibited by molybdate by more than 95%, regardless of sediment salinity. In the absence of inhibitor, high-salinity sediments methylated mercury at only 40% of the level observed in low-salinity sediments. In response to molybdate inhibition of sulfate reducers, methanogenesis increased up to 258% in high-salinity sediments but only up to 25% in low-salinity sediments. In contrast to an earlier low-salinity isolate, a Desulfovibrio desulfuricans strain from high-salinity sediment required 0.5 M sodium for optimal growth and mercury methylation activity. The formation of negatively charged mercuric chloride complexes at high salinity did not noticeably interfere with the methylation process. Results of these studies demonstrate that sulfate reducers are responsible for mercury methylation in anoxic estuarine sediments, regardless of the prevailing salinity.     

Mating system and multilocus associations in a natural population of Pseudotsuga menziesii (Mirb.) Franco

Summary Arrays of open-pollinated seeds were assayed for allozyme polymorphisms at ten loci (Aat2, Est1, G6pd, Idh, Mdh2, Mdh3, Pgm, Sod, 6Pgd1, 6Pgd2) to obtain estimates of the outcrossing rate and assess multilocus association in a natural population of coastal Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco. The allele frequencies in the samples of adult trees and pollen-gamete pool were similar. Maximum-likelihood estimators of the outcrossing rate for individual loci and two multilocus models were derived using counting methods. The single-locus maximum likelihood estimates (MLEs) of the outcrossing rate were significantly heterogeneous; they varied over a more than two-fold range from 0.404 to 0.935, with an average MLE of 0.741. Both multilocus MLEs of the outcrossing rate were 0.887. The sample of trees was in random mating equilibrium when assessed on a pairwise-locus basis using Burrows' composite measure of gametic disequilibrium, with one exception (Mdh2 Sod) that was attributable to a rare gametic class. In the sample of pollen gametes, 5 of the 45 pairwise-locus associations were nominally significant at the 0.05 level: Idh Est1, Mdh2 Sod, Aat2 Est1, Aat2 Mdh3, and Est1 Mdh3. These apparent associations were attributable in most cases to the relative excess of uncommon or rare paternal gametes of discernibly outcrossed embryos. An additional two-locus association was identified for Mdh2 Pgm which was marginally significant for the major partition of the contingency table that excluded paternal gametes with the rare allele Mdh2 ² .     

Molecular analysis of halophilic bacterial community for high-rate denitrification of saline industrial wastewater

A denitrification system for saline wastewater utilizing halophilic denitrifying bacteria has not been developed so far. In this study, denitrification performance and microbial community under various saline conditions were investigated using denitrifying sludge acclimated under low-salinity condition for a few years as seed sludge. A continuous denitrification experiment showed that denitrification performance and microbial community at 10% salinity was higher than that at 1% salinity. The microbial community in the denitrification sludge that was acclimated under low salinity was monitored by terminal-restriction fragment length polymorphism (T-RFLP) analysis during acclimation to high-salinity condition. T-RFLP profiles and clone analysis based on 16S rRNA-encoding genes in the sludge of the denitrification system with 10% salinity indicated that the γ-Proteobacteria, particularly Halomonas spp., were predominant species, suggesting that these bacterial members were possibly responsible for a high denitrification activity under high-salinity conditions. Furthermore, the investigation of denitrification performance under various saline conditions revealed that 4–10% salinity results in the highest denitrification rate, indicating that this salinity was optimal for predominant bacterial species to exhibit denitrification activity. These results indicate the possibility that an appropriate denitrification system for saline wastewater can be designed using acclimated sludge with a halophilic community.     

罗氏沼虾急性低温应激响应的转录组分析

为探究罗氏沼虾(Macrobrachium rosenbergii)急性低温应激响应的基因表达模式,研究以罗氏沼虾成虾为研究对象,分别设置实验组(16℃)和对照组(24℃),实验组从24℃急性降温(2℃/1h)至16℃后的0、1h、3h、6h、12h、24h和回温至24℃后共7个时间点采集肝胰腺组织进行转录组测序分析。差异表达基因(DEGs)筛选结果显示,胁迫3h与回温后的DEGs数量(5062个)几乎是1h与回温后的1.5倍(3516个),随着胁迫时间的延长,各时间点与回温后的DEGs数量逐渐降低。KEGG富集发现, DEGs显著富集在溶酶体、淀粉和蔗糖代谢、抗原加工与呈递等途径中。此外,罗氏沼虾在急性低温应激下,黏着斑、ECM-受体互作、细胞色素P450对异生物质的代谢、谷胱甘肽代谢、氧化磷酸化、p53信号通路等相关基因也发生了显著变化。WGCNA分析发现各组间的共有DEGs聚类在与细胞功能及免疫相关模块和与能量物质代谢相关模块上。另外,花生四烯酸代谢信号通路中的Cytochrome P450 2L1-like基因、谷胱甘肽代谢信号通路中的NADP-specific isocitr...