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1.
A transient packaging method for a retroviral vector containing β-globin sequences, mediated by Ca3(PO4)2 precipitation, generated stable recombinant β-globin retroviruses with intact viral genomes. We suggest the use of this method
as an alternative means to overcome the production of β-globin encoding retroviruses with aberrant viral genomes using the
routine packaging procedure. 相似文献
2.
Ma XD Ke T Li YX Huang CX Song AD Chen HG Wu YH Jia XC He GY 《Biotechnology letters》2004,26(2):171-175
Three mutants of the wild type alpha-amylase gene from Xanthomonas campestris pv. campestris 8004 were obtained using a PCR technique in which deoxythymidine triphosphate (dTTP) was partially replaced by 5-bromo-2'-deoxyuridine-5'-triphosphate (BrdUTP), at an optimal dTTP:BrdUTP ratio of 1000:1. Of thre three mutants that were obtained and which were sequenced, one mutant with 40 times higher activity than the wild type alpha-amylase gene product was obtained by using primary PCR products as a template for a second PCR reaction. 相似文献