共查询到20条相似文献,搜索用时 15 毫秒
1.
Leal WS Barbosa RM Xu W Ishida Y Syed Z Latte N Chen AM Morgan TI Cornel AJ Furtado A 《PloS one》2008,3(8):e3045
Synthetic mosquito oviposition attractants are sorely needed for surveillance and control programs for Culex species, which are major vectors of pathogens causing various human diseases, including filariasis, encephalitis, and West Nile encephalomyelitis. We employed novel and conventional chemical ecology approaches to identify potential attractants, which were demonstrated in field tests to be effective for monitoring populations of Cx. p. quinquefasciatus in human dwellings. Immunohistochemistry studies showed that an odorant-binding protein from this species, CquiOBP1, is expressed in trichoid sensilla on the antennae, including short, sharp-tipped trichoid sensilla type, which house an olfactory receptor neuron sensitive to a previously identified mosquito oviposition pheromone (MOP), 6-acetoxy-5-hexadecanolide. CquiOBP1 exists in monomeric and dimeric forms. Monomeric CquiOBP1 bound MOP in a pH-dependent manner, with a change in secondary structure apparently related to the loss of binding at low pH. The pheromone antipode showed higher affinity than the natural stereoisomer. By using both CquiOBP1 as a molecular target in binding assays and gas chromatography-electroantennographic detection (GC-EAD), we identified nonanal, trimethylamine (TMA), and skatole as test compounds. Extensive field evaluations in Recife, Brazil, a region with high populations of Cx. p. quinquefasciatus, showed that a combination of TMA (0.9 microg/l) and nonanal (0.15 ng/microl) is equivalent in attraction to the currently used infusion-based lure, and superior in that the offensive smell of infusions was eliminated in the newly developed synthetic mixture. 相似文献
2.
Nabaneeta Saha Gautam Aditya Goutam K. Saha Stephanie E. Hampton 《Aquatic Ecology》2010,44(1):167-176
Tropical aquatic environments host a large number of predatory insects including heteropteran water bugs Anisops bouvieri Kirkaldy, 1704 (Heteroptera: Notonectidae), Diplonychus (=Sphaerodema) rusticus Fabricius, 1781 (Heteroptera: Belostomatidae), and Diplonychus (=Sphaerodema) annulatus Fabricius, 1781 (Heteroptera: Belostomatidae) feeding on a range of organisms. In tropical and subtropical wetlands, ponds,
and temporary pools these predators play a role in regulation of dipteran populations, particularly mosquitoes and chironomids.
Their relative abilities to control mosquitoes depend in part on predator preference for mosquitoes in relation to other natural
prey, and the predators’ propensities to switch to mosquitoes as mosquito density increases. The prey electivity and switching
dynamics of these predatory water bugs were evaluated in the laboratory under various prey densities, using two instars (II
and IV) of chironomid and mosquito larvae as prey. Studies of electivity at relatively high densities (20 prey L−1) in small (5 L) vessels demonstrated that all predators showed opportunistic foraging as the mosquito:chironomid ratio changed,
with some evidence that mosquito larvae were positively selected over chironomids. In particular, Anisops showed strong electivity for mosquitoes when presented with any ratio of large mosquito and chironomid prey in the high density
experiment, although the preference was not expressed in lower density (2.5 prey L−1) treatments executed in 40 L vessels. In these lower density treatments, D. rusticus demonstrated higher electivity for mosquitoes when the mosquito:chironomid ratio was high, consistent with non-significant
trends observed in the higher density experiment. The positive electivity of D. rusticus for mosquitoes was reinforced in an experiment executed over 16 days at varying prey ratios, in which D. rusticus mosquito electivity was high and consistent while D. annulatus showed slight avoidance of mosquito larvae, and Anisops remained largely opportunistic in foraging on prey in proportion with availability. Anisops and D. rusticus are potentially good biocontrol agents for mosquito larvae, in that they preferentially consume mosquitoes under many circumstances
but can readily forage on other prey when mosquito density is low. 相似文献
3.
A new cell line, UM-AVE1, was established from embryos of the mosquito Aedes vexans. Banding patterns for the isozymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), xanthine dehydrogenase (XDH), and esterases were compared with those of larval Aedes vexans tissues as well as those of four other mosquito cell lines and one moth cell line. Karyotype analyses confirmed that the dipteran cell lines were not contaminated with lepidopteran cells, because in all mosquito lines the modal number of chromosomes was 6 (=2n) or 7. Isozyme electrophoresis established a specific profile for each cell line. Two isozymes present in UM-AVE1 (LDH, IDH) were not detected in larvae; this could be a reflection of the different stages used for cell line isolation and enzyme analysis, or lability of sample preparations. It is significant that extracts from UM-AVE1 cells and Aedes vexans larvae had an identical double band for XDH, while all other cell lines examined exhibited only a single band. 相似文献
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A. Abdel-Hameed J. Kiviranta K. Sivonen S. Niemelä G. Carlberg 《World journal of microbiology & biotechnology》1993,9(2):156-159
The presence of cyanobacteria generally decreased the effectiveness of Bacillus thuringiensis H-14 (BTI) as a mosquito larvicide. The effect was more pronounced when the mosquito larvae were exposed to BTI in the presence of several cyanobacterial strains. No synergistic or antagonistic effect between the -endotoxin from BTI and the hepatotoxin from cyanobacteria was seen. Neurotoxic cyanobacterial strains caused very fast paralysis in mosquito larvae; the decreases in the effectiveness of BTI when tested in combination with a neurotoxic strain might be due to the effect of this paralytic action on the feeding rate of the mosquito larvae. 相似文献
6.
Jian Zhang Fu Sheng Huang Wen Yue Xu Ying Wang Tao Li Zhou Jian Hua Duan 《Experimental parasitology》2011,(1):52-57
The antimalarial drug nitroquine is not only an effective antimalarial drug, it is also able to induce the melanization of Plasmodium species. However, the molecular mechanisms of the recognition reaction induced by this drug remain unclear. Silencing of thioester-containing protein-1 (TEP1) significantly compromised the ability of Anopheles gambiae to melanize the Plasmodium, leading to investigation of the involvement of A. stephensi TEP1 in melanization induced by nitroquine. This study shows that (1) binding of AsTEP1 to oocysts, especially melanized oocysts, (2) after ingestion of anti-AsTEP1 antibody, the melanization rate in antibody-treated mosquitoes are significantly lower than in control mosquito (p < 0.05). The results suggest that nitroquine is able to induce Plasmodium recognition by TEP1, possibly triggering the resulting melanotic encapsulation. Further elucidation of the molecular mechanisms of mosquito immunity induced by antimalarial drugs will provide theoretical evidence for the use of antimalarial drugs, and a meaningful pathway for the design of novel antimalarial drugs. 相似文献
7.
Xianzhong Xu Yun Li Josep Rayo Yuko Ishida Walter Leal James B. Ames 《Biomolecular NMR assignments》2008,2(2):105-106
A pheromone-binding protein from navel orange worm, Amyelois transitella (Atra-PBP1) binds to non-polar pheromone molecules and facilitates the transport and delivery of pheromone to the membrane-bound
pheromone receptors. We report complete NMR chemical shift assignments of Atra-PBP1 obtained at pH 4.5 and 25°C (BMRB No.
15601). 相似文献
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Serge Michalet Guillaume Minard Wilfried Chevalier Guillaume Meiffren Yoann Saucereau Van Tran Van Gilles Comte Florence-Hélène Tran Claire Valiente Moro 《Environmental microbiology》2019,21(12):4662-4674
Aedes albopictus is a vector of arboviruses and filarial nematodes. Originating from Asia, this mosquito has rapidly expanded its geographical distribution and colonized areas across both temperate and tropical regions. Due to the increase in insecticide resistance, the use of environmentally friendly vector control methods is encouraged worldwide. Using methods based on semiochemicals in baited traps are promising for management of mosquito populations. Interestingly, human skin microbiota was shown to generate volatile compounds that attract the mosquito species Anopheles gambiae and Aedes aegypti. Here, we investigated the composition of skin bacteria from different volunteers and the attractive potential of individual isolates to nulliparous Ae. albopictus females. We showed that three out of 16 tested isolates were more attractive and two were more repulsive. We identified dodecenol as being preferentially produced by attractive isolates and 2-methyl-1-butanol (and to a lesser extent 3-methyl-1-butanol) as being overproduced by these isolates compared with the other ones. Those bacterial volatile organic compounds represent promising candidates but further studies are needed to evaluate their potential application for baited traps improvement. 相似文献
10.
Ekbote U Looker M Isaac RE 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2003,134(4):593-598
Angiotensin-converting enzyme (ACE) is a dipeptidyl carboxypeptidase, which cleaves dipeptides and, in some instances, dipeptide or tripeptide amides from the C-terminus of regulatory peptides (e.g. angiotensin I, bradykinin and substance P). The expression of ACE is highly regulated in insects, where it is thought to have a role in the metabolism of peptide hormones involved in regulating reproduction. After a blood meal, ACE activity in the female mosquito Anopheles stephensi, increases four-fold with much of the enzyme finally accumulating in the ovary. In the present study, we have studied the effect on reproduction of adding two selective inhibitors of ACE, captopril and lisinopril, to the blood meal. Both ACE inhibitors reduced the size of the batch of eggs laid by females in a dose-dependent manner, with no observable effects on the behaviour of the adult insect. The almost total failure to lay eggs after feeding on either 1 mM captopril or 1 mM lisinopril, did not result from interference with the development of the primary follicle, but was due to the inhibition of egg-laying. Since very similar effects on the size of the egg-batch were observed with two selective ACE inhibitors, belonging to different chemical classes, we suggest that these effects are mediated by the selective inhibition of the induced mosquito ACE, a peptidase probably involved in the activation/inactivation of a peptide regulating egg-laying activity in A. stephensi. 相似文献
11.
A member of the Tc1 family of transposable elements has been identified in the Central and South American mosquito Anopheles albimanus. The full-length Quetzal element is 1680 base pairs (bp) in length, possesses 236 bp inverted terminal repeats (ITRs), and has a single open reading frame (ORF) with the potential of encoding a 341-amino-acid (aa) protein that is similar to the transposases of other members of the Tc1 family, particularly elements described from three different Drosophila species. The approximately 10–12 copies per genome of Quetzal are found in the euchromatin of all three chromosomes of A. albimanus. One full-length clone, Que27, appears capable of encoding a complete transposase and may represent a functional copy of this element. 相似文献
12.
Jianxin Sun Tsuyoshi Hiraoka Neal T. Dittmer Kook-Ho Cho Alexander S. Raikhel 《Insect biochemistry and molecular biology》2000,30(12)
We examined expression of the lipophorin (Lp) gene, lipophorin (Lp) synthesis and secretion in the mosquito fat body, as well as dynamic changes in levels of this lipoprotein in the hemolymph and ovaries, during the first vitellogenic cycle of females of the yellow fever mosquito, Aedes aegypti. Lipophorin was purified by potassium bromide (KBr) density gradient ultracentrifugation and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). Polyclonal antibodies were produced against individual Lp apoproteins, apolipoprotein-I (apoLp-I) and apolipoprotein-II (apoLp-II), with molecular weights of 240 and 75 kDa, respectively. We report here that in the mosquito A. aegypti, Lp was synthesized by the fat body, with a low level of the Lp gene expression and protein synthesis being maintained in pre- and postvitellogenic females. Following a blood meal, the Lp gene expression and protein synthesis were significantly upregulated. Our findings showed that the fat body levels of Lp mRNA and the rate of Lp secretion by this tissue reached their maximum at 18 h post-blood meal (PMB). 20-Hydroxyecdysone was responsible for an increase in the Lp gene expression and Lp protein synthesis in the mosquito fat body. Finally, the immunocytochemical localization of Lp showed that in vitellogenic female mosquitoes, this protein was accumulated by developing oocytes where it was deposited in yolk granules. 相似文献
13.
Liangbiao Zheng 《Archives of insect biochemistry and physiology》1997,34(1):1-18
The success of vector borne disease transmission depends on the interplay between mosquito and pathogen. Understanding the genetic and molecular basis of refractoriness of mosquito may lead to novel disease control mechanisms. To complete the life cycle within the vector mosquito, a pathogen needs to overcome several physical barriers, such as the peritrophic matrix, midgut epithelium, or salivary glands. The immune response of the mosquito has to be neutralized or avoided. Genomic approaches are being employed to identify the genetic and molecular differences between selected strains of refractory and susceptible mosquitoes. Detailed molecular genetic maps based on restriction fragment length polymorphism (RFLP) and microsatellite (or simple sequence repeat) markers have been developed for two important vectors, Aedes aegypti and Anopheles gambiae, respectively. Recent success in genetic localization of quantitative trait loci controlling refractoriness/susceptibility of mosquitoes for Plasmodium and Brugian microfilariae provides a framework for further molecular characterization. Libraries of large genomic DNA inserts in bacterial or yeast artificial chromosomes (BACs and YACs) will facilitate physical mapping of the genetic loci controlling refractoriness. Identification of candidate refractory genes, and the cloning of other molecular markers for the mosquito immunity, provides tools for fruitful analysis of mosquito-parasite interactions. © 1997 Wiley-Liss, Inc. 相似文献
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A local population of the pitcher-plant mosquito, Wyeomyia smithii (Diptera: Culicidae, Culicini) in western New York State contains naturally polymorphic salivary gland chromosomes. Maps depicting the proposed standard sequence of bands along each of the three chromosomes are presented. Structural conformations of heterokaryotypes reveal ten paracentric inversions. Four inversions occur in chromosome 1, and three in each of chromosomes 2 and 3. All the inversions are small, occupying 3–17% of the length of their respective chromosomes, and all are located in the terminal 1/5 to 1/2 of a chromosome arm. Three inversions are almost adjacent on chromosome 1. On chromosome 2 one inversion lies completely within another. Three inversions on chromosome 3 partially overlap. The remaining two inversions occur alone, one on chromosome 1 and the other on chromosome 2. Another polymorphism, with no visible alteration of band sequence, produces asynapsis of all but the tip of the left arm of chromosome 1, and shows a significant change in frequency between two successive years. No inversion exhibits a significant seasonal change in frequency, though some fluctuation in frequency was noted in two cases. The rarest inversion in the population exists at a frequency below 0.01, and the two most common inversions at frequencies of 0.44 and 0.52. The average inversion heterozygosity of an individual is between 1.7 and 2.4 based on two different estimation procedures. Heterokaryotypes for several of the inversions were significantly more frequent than they should have been, given binomial expectations. Cytogenetic analysis of W. smithii was deemed particularly desirable because this species has been and continues to be the subject of extensive biometrical-genetic, ecological, and evolutionary investigation.This paper is the seventh in a series on the ecology and evolution of the pitcher-plant mosquito 相似文献
16.
Climate can strongly influence the population dynamics of disease vectors and is consequently a key component of disease ecology.
Future climate change and variability may alter the location and seasonality of many disease vectors, possibly increasing
the risk of disease transmission to humans. The mosquito species Culex quinquefasciatus is a concern across the southern United States because of its role as a West Nile virus vector and its affinity for urban
environments. Using established relationships between atmospheric variables (temperature and precipitation) and mosquito development,
we have created the Dynamic Mosquito Simulation Model (DyMSiM) to simulate Cx. quinquefasciatus population dynamics. The model is driven with climate data and validated against mosquito count data from Pasco County, Florida
and Coachella Valley, California. Using 1-week and 2-week filters, mosquito trap data are reproduced well by the model (P < 0.0001). Dry environments in southern California produce different mosquito population trends than moist locations in Florida.
Florida and California mosquito populations are generally temperature-limited in winter. In California, locations are water-limited
through much of the year. Using future climate projection data generated by the National Center for Atmospheric Research CCSM3
general circulation model, we applied temperature and precipitation offsets to the climate data at each location to evaluate
mosquito population sensitivity to possible future climate conditions. We found that temperature and precipitation shifts
act interdependently to cause remarkable changes in modeled mosquito population dynamics. Impacts include a summer population
decline from drying in California due to loss of immature mosquito habitats, and in Florida a decrease in late-season mosquito
populations due to drier late summer conditions. 相似文献
17.
When treated with the steroid hormone 20‐hydroxyecdysone (20E), C7‐10 cells from the mosquito, Aedes albopictus, arrest in the G1 phase of the cell cycle. To explore whether 20E‐mediated cell cycle arrest proceeds through increased levels of cell cycle inhibitor (CKI) proteins, we cloned the Ae. albopictus homolog of dacapo, the single member of the Cip/Kip family of CKI proteins known from Drosophila melanogaster. The Ae. albopictus dacapo cDNA encoded a 261‐amino acid homolog of the Aedes aegypti protein XP_001651102.1, which is encoded by an ~23 kb gene containing three exons. Like dacapo from D. melanogaster, the ~27 kDa protein from Aedes and Culex mosquitoes contained several S/TXXE/D motifs corresponding to potential protein kinase CK2 phosphorylation sites, and a binding site for proliferating cell nuclear antigen (PCNA). When extracts from cells treated with 20E were analyzed by western blotting, using a primary antibody to synthetic peptides from the mosquito dacapo protein, up‐regulation of an ~27 kDa protein was observed within 24 h, and the abundance of the protein further increased by 48 h after hormone treatment. This is the first investigation of a cell cycle inhibitory protein in mosquitoes. The results reinforce growing evidence that 20E affects expression of proteins that regulate cell cycle progression. © 2011 Wiley Periodicals, Inc. 相似文献
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19.
Sinden RE 《Parassitologia》1999,41(1-3):139-148
The essential passage of the malarial parasite through a mosquito vector results in major population bottlenecks in parasite numbers. The volume of the bloodmeal ingested by the female mosquito is 1-2 microliters. This may contain from 1 to 10(5) gametocytes. Of these, it is normal for just 12 to become macrogametes; 5-6 become ookinetes, and 2 develop into oocysts 2-7 days later. Of the 16,000 sporozoites produced from these two oocysts just 10-20 are inoculated by the malaria-infected female mosquito each time she probes when taking a subsequent bloodmeal. These significant population bottlenecks suggest that parasite differentiation is severely constrained by the environment in the mosquito, and therefore by the interactions between the parasite and the vector. This review will describe parasite differentiation in the mosquito and try to highlight the more important interactions between the parasite, the bloodmeal and the mosquito, attempting to identify those interactions which are essential to parasite differentiation, and those where the mosquito may be mounting effective strategies against this important pathogen. The potential exploitation of these interactions as possible mechanisms for intervention will be discussed. 相似文献
20.
《Bioscience, biotechnology, and biochemistry》2013,77(10):2544-2546
A pair of enantiomers of trans-p-menth-2-en-1-ol, an aggregation pheromone of Platypus quercivorus, was synthesized from (S)- and (R)-limonene. The retention time of the aggregation pheromone from the insect coincided with that of (1S,4R)-p-menth-2-en-1-ol synthesized from (S)-limonene from GC analyses with a chiral column, enabling the absolute configuration of the aggregation pheromone to be determined as (1S,4R). 相似文献