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1.
Bachoon DS Araujo R Molina M Hodson RE 《Journal of industrial microbiology & biotechnology》2001,27(2):72-79
Microbial community dynamics in wetlands microcosms emended with commercial products (surfactant, a biological agent, and
nutrients) designed to enhance bioremediation was followed for 3 months. The effectiveness of enhanced degradation was assessed
by determining residual concentrations of individual petroleum hydrocarbons by GC/MS. The size and composition of the sediment
microbial community was assessed using a variety of indices, including bacterial plate counts, MPNs, and DNA hybridizations
with domain- and group-specific oligonucleotide probes. The addition of inorganic nutrients was the most effective treatment
for the enhancement of oil degradation, resulting in marked degradation of petroleum alkanes and a lesser extent of degradation
of aromatic oil constituents. The enhanced degradation was associated with increases in the amount of extractable microbial
DNA and Streptomyces in the sediment, although not with increased viable counts (plate counts, MPN). Bacteria introduced with one of the proprietary
products were still detected in the microcosms after 3 months, but were not a major quantitative constituent of the community.
The biological product enhanced oil degradation relative to the control, but to a lesser extent than the nutrient additions
alone. In contrast, application of the surfactant to the oil-impacted sediment decreased oil degradation. Journal of Industrial Microbiology & Biotechnology (2001) 27, 72–79.
Received 18 March 2001/ Accepted in revised form 09 June 2001 相似文献
2.
Aerobic microbial degradation of pollutant oil (petroleum) in aquatic environments is often severely limited by the availability of combined nitrogen. We therefore studied whether the microbial community enriched in marine sediment microcosms with an added oil layer and exposure to light harboured nitrogenase activity. The acetylene reduction (AR) assay indeed indicated active nitrogenase; however, similar activity was observed in oil-free control microcosms. In both microcosms, the AR rate was significantly reduced upon a dark shift, indicating that enriched cyanobacteria were the dominant diazotrophs. Analysis of structural dinitrogenase reductase genes (nifH) amplified from both microcosms indeed revealed NifH sequences related mostly to those of heterocystous cyanobacteria. NifH sequences typically affiliating with those of heterotrophic bacteria were more frequently retrieved from the oil-containing sediment. Expression analyses showed that mainly nifH genes similar to those of heterocystous cyanobacteria were expressed in the light. Upon a dark shift, nifH genes related to those of non-heterocystous cyanobacteria were expressed. Expression of nifH assignable to heterotrophs was apparently not significant. It is concluded that cyanobacteria are the main contributors of fixed nitrogen to oil-contaminated and pristine sediments if nitrogen is a limiting factor and if light is available. Hence, also the oil-degrading heterotrophic community may thus receive a significant part of combined nitrogen from cyanobacteria, even though oil vice versa apparently does not stimulate an additional nitrogen fixation in the enriched community. 相似文献
3.
Naphthalene biodegradation was investigated in microcosms containing sediment and water collected from three ecosystems which varied in past exposure to anthropogenic and petrogenic chemicals. Mineralization half-lives for naphthalene in microcosms ranged from 2.4 weeks in sediment chronically exposed to petroleum hydrocarbons to 4.4 weeks in sediment from a pristine environment. Microbiological analysis of sediments indicated that hydrocarbon-utilizing microbial populations also varied among ecosystems and were 5 to 12 times greater in sediment after chronic petrogenic chemical exposure than in sediment from an uncontaminated ecosystem. Sediment from an ecosystem exposed to agricultural chemicals had a mineralization half-life of 3.2 weeks for naphthalene and showed about a 30-fold increase in heterotrophic bacterial populations in comparison to uncontaminated sediments, but only a 2- to 3-fold increase in hydrocarbon-degrading bacteria. Analysis of organic solvent-extractable residues from the microcosms by high-pressure liquid chromatography detected polar metabolites which accounted for 1 to 3% of the total radioactivity. Purification of these residues by thin-layer chromatography and further analysis by gas chromatography-mass spectrometry indicated that cis-1,2-dihydroxy-1,2-dihydronaphthalene, 1-naphthol, salicylic acid, and catechol were metabolites of naphthalene. These results provide useful estimates for the rates of naphthalene mineralization in different natural ecosystems and on the degradative pathway for microbial metabolism of naphthalene in freshwater and estuarine environments. 相似文献
4.
Naphthalene biodegradation in environmental microcosms: estimates of degradation rates and characterization of metabolites. 总被引:10,自引:9,他引:1
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Naphthalene biodegradation was investigated in microcosms containing sediment and water collected from three ecosystems which varied in past exposure to anthropogenic and petrogenic chemicals. Mineralization half-lives for naphthalene in microcosms ranged from 2.4 weeks in sediment chronically exposed to petroleum hydrocarbons to 4.4 weeks in sediment from a pristine environment. Microbiological analysis of sediments indicated that hydrocarbon-utilizing microbial populations also varied among ecosystems and were 5 to 12 times greater in sediment after chronic petrogenic chemical exposure than in sediment from an uncontaminated ecosystem. Sediment from an ecosystem exposed to agricultural chemicals had a mineralization half-life of 3.2 weeks for naphthalene and showed about a 30-fold increase in heterotrophic bacterial populations in comparison to uncontaminated sediments, but only a 2- to 3-fold increase in hydrocarbon-degrading bacteria. Analysis of organic solvent-extractable residues from the microcosms by high-pressure liquid chromatography detected polar metabolites which accounted for 1 to 3% of the total radioactivity. Purification of these residues by thin-layer chromatography and further analysis by gas chromatography-mass spectrometry indicated that cis-1,2-dihydroxy-1,2-dihydronaphthalene, 1-naphthol, salicylic acid, and catechol were metabolites of naphthalene. These results provide useful estimates for the rates of naphthalene mineralization in different natural ecosystems and on the degradative pathway for microbial metabolism of naphthalene in freshwater and estuarine environments. 相似文献
5.
2,4,6-Trinitrotoluene (TNT) is a toxic and persistent explosive compound occurring as a contaminant at numerous sites worldwide. Knowledge of the microbial dynamics driving TNT biodegradation is limited, particularly in native aquifer sediments where it poses a threat to water resources. The purpose of this study was to quantify the effect of organic amendments on anaerobic TNT biodegradation rate and pathway in an enrichment culture obtained from historically contaminated aquifer sediment and to compare the bacterial community dynamics. TNT readily biodegraded in all microcosms, with the highest biodegradation rate obtained under the lactate amended condition followed by ethanol amended and naturally occurring organic matter (extracted from site sediment) amended conditions. Although a reductive pathway of TNT degradation was observed across all conditions, denaturing gradient gel electrophoresis (DGGE) analysis revealed distinct bacterial community compositions. In all microcosms, Gram-negative γ- or β-Proteobacteria and Gram-positive Negativicutes or Clostridia were observed. A Pseudomonas sp. in particular was observed to be stimulated under all conditions. According to non-metric multidimensional scaling analysis of DGGE profiles, the microcosm communities were most similar to heavily TNT-contaminated field site sediment, relative to moderately and uncontaminated sediments, suggesting that TNT contamination itself is a major driver of microbial community structure. Overall these results provide a new line of evidence of the key bacteria driving TNT degradation in aquifer sediments and their dynamics in response to organic carbon amendment, supporting this approach as a promising technology for stimulating in situ TNT bioremediation in the subsurface. 相似文献
6.
Use of isotopic and molecular techniques to link toluene degradation in denitrifying aquifer microcosms to specific microbial populations 总被引:1,自引:0,他引:1
Pelz O Chatzinotas A Andersen N Bernasconi SM Hesse C Abraham WR Zeyer J 《Archives of microbiology》2001,175(4):270-281
Microcosms were inoculated with sediments from both a petroleum-hydrocarbon (PHC)-contaminated aquifer and from a nearby pristine aquifer and incubated under anoxic denitrifying conditions with [methyl-13C]toluene. These microcosms served as a laboratory model system to evaluate the combination of isotope (13C-labeling of polar-lipid-derived fatty acids) and molecular techniques (16S rRNA-targeting gene probes) to identify the toluene-metabolizing population. After total depletion of toluene, the following bacterial phospholipid fatty acids (PLFA) were 13C-enriched: 16:1omega7c, 16:1omega7t, 16:0, cy17:0, and 18:1omega7c. Pure culture experiments demonstrated that these compounds were also found in PLFA profiles of PHC-degrading Azoarcus spp. (beta-Proteobacteria) and related species. The origin of the CO2 evolved in the microcosms was determined by measurements of stable carbon isotope ratios. Toluene represented 11% of the total pool of mineralized substrates in the contaminated sediment and 54% in the pristine sediment. The microbial community in the microcosm incubations was characterized by using DAPI staining and whole-cell hybridization with specific fluorescently labeled 16S rRNA-targeted oligonucleotide probes. Results revealed that 6% of the DAPI-stained cells in the contaminated sediment and 32% in the pristine sediment were PHC-degrading Azoarcus spp. In biotic control microcosms (incubated under denitrifying conditions, no toluene added), Azoarcus spp. cells remained at less than 1% of the DAPI-stained cells. The results show that isotope analysis in combination with whole-cell hybridization is a promising approach to identify and to quantify denitrifying toluene degraders within microbial communities. 相似文献
7.
Srinivasa Varadhan A Khodadoust AP Brenner RC 《Journal of industrial microbiology & biotechnology》2011,38(10):1691-1707
Reductive dehalogenation of polychlorinated biphenyls (PCBs) by indigenous dehalorespiring microorganisms in contaminated
sediments may be enhanced via biostimulation by supplying hydrogen generated through the anaerobic corrosion of elemental
iron added to the sediment. In this study, the effect of periodic amendment of sediment with various dosages of iron on the
microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months.
Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis
of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with
the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower
microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months,
thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers.
The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities
in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early
prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration
of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population
of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. Biostimulation of indigenous PCB dechlorinators
by the periodic amendment of contaminated sediments with low dosages of iron metal may therefore be an effective technology
for remediation of PCB-contaminated sediments. 相似文献
8.
Rønn R McCaig AE Griffiths BS Prosser JI 《Applied and environmental microbiology》2002,68(12):6094-6105
The influence of grazing by a mixed assemblage of soil protozoa (seven flagellates and one amoeba) on bacterial community structure was studied in soil microcosms amended with a particulate resource (sterile wheat roots) or a soluble resource (a solution of various organic compounds). Sterilized soil was reinoculated with mixed soil bacteria (obtained by filtering and dilution) or with bacteria and protozoa. Denaturing gradient gel electrophoresis (DGGE) of PCR amplifications of 16S rRNA gene fragments, as well as community level physiological profiling (Biolog plates), suggested that the mixed protozoan community had significant effects on the bacterial community structure. Excising and sequencing of bands from the DGGE gels indicated that high-G+C gram-positive bacteria closely related to Arthrobacter spp. were favored by grazing, whereas the excised bands that decreased in intensity were related to gram-negative bacteria. The percentages of intensity found in bands related to high G+C gram positives increased from 4.5 and 12.6% in the ungrazed microcosms amended with roots and nutrient solution, respectively, to 19.3 and 32.9% in the grazed microcosms. Protozoa reduced the average bacterial cell size in microcosms amended with nutrient solution but not in the treatment amended with roots. Hence, size-selective feeding may explain some but not all of the changes in bacterial community structure. Five different protozoan isolates (Acanthamoeba sp., two species of Cercomonas, Thaumatomonas sp., and Spumella sp.) had different effects on the bacterial communities. This suggests that the composition of protozoan communities is important for the effect of protozoan grazing on bacterial communities. 相似文献
9.
Characterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation 总被引:1,自引:0,他引:1
Li-Ying WangCai-Xia Gao Serge Maurice MbadingaLei Zhou Jin-Feng LiuJi-Dong Gu Bo-Zhong Mu 《International biodeterioration & biodegradation》2011,65(3):444-450
Oil reservoirs represent special habitats for the activity of anaerobic microbial communities in the transformation of organic compounds. To understand the function of microbial communities in oil reservoirs under anaerobic conditions, an alkane-degrading methanogenic enrichment culture was established and analyzed. Results showed that a net 538 ??mol of methane higher than the controls were produced over 274 days of incubation in microcosms amended with alkanes and a decrease in the alkanes profile was also observed. Phylogenetic analysis of 16S rRNA gene sequences retrieved from the enrichment microcosms indicated that the archaeal phylotypes were mostly related to members of the orders Methanobacteriales and Methanosarcinales. The bacterial clone library was composed of sequences affiliated with the Firmicutes, Proteobacteria, Deferribacteres, and Bacteroidetes. However, most of the bacterial clones retrieved from the enrichment cultures showed low similarity to 16S rRNA gene sequences of the cultured members, indicating that the enrichment cultures contained novel bacterial species. Though alkane-degrading methanogenic enrichment consortium has rarely been reported from petroleum reservoirs, our results indicated that oilfield production water harbors a microbial community capable of syntrophic conversion of n-alkanes to methane, which sheds light on the bio-utilization of marginal oil reservoirs for enhanced energy recovery. 相似文献
10.
Structure of bacterial communities along a hydrocarbon contamination gradient in a coastal sediment 总被引:2,自引:0,他引:2
Paissé S Coulon F Goñi-Urriza M Peperzak L McGenity TJ Duran R 《FEMS microbiology ecology》2008,66(2):295-305
The bacterial diversity of a chronically oil-polluted retention basin sediment located in the Berre lagoon (Etang-de-Berre, France) was investigated. This study combines chemical and molecular approaches in order to define how the in situ petroleum hydrocarbon contamination level affects the bacterial community structure of a subsurface sediment. Hydrocarbon content analysis clearly revealed a gradient of hydrocarbon contamination in both the water and the sediment following the basin periphery from the pollution input to the lagoon water. The nC17 and pristane concentrations suggested alkane biodegradation in the sediments. These results, combined with those of terminal-restriction fragment length polymorphism analysis of the 16S rRNA genes, indicated that bacterial community structure was obviously associated with the gradient of oil contamination. The analysis of bacterial community composition revealed dominance of bacteria related to the Proteobacteria phylum (Gamma-, Delta-, Alpha-, Epsilon- and Betaproteobacteria), Bacteroidetes and Verrucomicrobium groups and Spirochaetes, Actinobacteria and Cyanobacteria phyla. The adaptation of the bacterial community to oil contamination was not characterized by dominance of known oil-degrading bacteria, because a predominance of populations associated to the sulphur cycle was observed. The input station presented particular bacterial community composition associated with a low oil concentration in the sediment, indicating the adaptation of this community to the oil contamination. 相似文献
11.
We tested the effects of four different sediment types collected from northern Gulf of Mexico estuarine systems on macroinfaunal colonization and community development in our laboratory flow-through microcosm system. Four sediments, types included , a beach sand, two fine-grained muds, but from different locations, and a 50:50 mixture of one of the mud sediments and beach sand. Our hypothesis was that the pattern of colonization would differ among sediment types based on empirical field data and theory but the differences would be expressed most strongly at sediment type extremes (e.g., mud versus sand). A total of 49 taxa colonized the four sediments. Unidentified Actiniaria (sea anemones) numerically dominated densities among all four sediments with densities ranging between 46.5 to 60.5 per microcosm (20 cm side–1). Average taxa richness per microcosm (N: 10 replicates per sediment treatment) ranged from 10.4 in one of the mud treatments to 14.9 taxa in the sand. These were the only significant differences among sediment types (P0.05) in taxa richness and we detected no significant effects of sediment type on animal densities. Differences in community metrics, although statistically significant, were generally of a relatively small magnitude. Five of 10 microcosms per treatment were randomly selected to test for effects of sediment depth (e.g., top, mid, and bottom). In vertically sectioned microcosms, average taxa richness in sand treatments was significantly greater than those of the other three sediments. A non-parametric multivariate analysis (Primer) revealed that community structure in the vertically sectioned microcosms differed significantly between sand and one of the mud treatments. Mean taxa richness of top sections differed significantly from mid and bottom sections. We detected significantly higher animal densities and taxa richness (p0.05) in vertically sectioned versus non-sectioned microcosms. However, these differences were unexplained based on experimental protocols. 相似文献
12.
Anna Reunamo Pirjo Yli-Hemminki Jari Nuutinen Jouni Lehtoranta Kirsten S. Jørgensen 《Geomicrobiology journal》2017,34(5):385-399
The ability of different sea bottom types to recover from oil contamination under oxic and anoxic conditions is poorly known in the brackish Baltic Sea. We carried out microcosm experiments to examine the capacity of iron–manganese concretions and sediment to remove petroleum compounds at 10°C. The biological degradation potential of polycyclic aromatic hydrocarbons (PAHs) was indicated by the 1000-fold increase in the copy numbers of PAH-degradation genes resulting in 2.2 × 106 gene copies g?1 DW. In the experiments 35?81% of the crude oil and 96% of PAHs disappeared through abiotic and biotic pathways, and there was little difference between the concretions and sediment. Bacterial community analysis revealed that the bacterial sequences obtained from the oil-treated concretions were affiliated to groups typical for concretions and metal-rich environments, whereas oil-treated sediment sequences were similar to those originating from sediments and oil-contaminated environments. The high frequency of concretion clones affiliated with specific taxonomic groups of α-, β-, γ-, and δ-proteobacteria may indicate the existence of new clades of bacteria within the Rhizobiales and Rhodospirillales of the α-proteobacteria, Burkholderiales—incertae sedis of the β-proteobacteria, Chromatiales of the γ-proteobacteria and the Syntrophobacteriales of the δ-proteobacteria. Our study suggests that concretions and bottom sediment maintain rich, distinct bacterial communities under oil exposure that have the potential to remove petroleum compounds in oxic and anoxic conditions. 相似文献
13.
Influence of TNT transformation on microbial community structure in four different lake microcosms 总被引:3,自引:0,他引:3
Wikström P Andersson AC Nygren Y Sjöström J Forsman M 《Journal of applied microbiology》2000,89(2):302-308
After World War II, large amounts of obsolete ammunition were dumped in various lakes in Sweden. Trinitrotoluene, TNT, was one of the main components of the dumped explosives. In this study, four different lake microcosms originating from lakes where relatively large amounts of ammunition were dumped were used to mimic the effect of TNT release on the natural microbial community. Increased microbial growth was found in lake microcosms amended with TNT. However, negligible mineralization of TNT was detected, suggesting that TNT was not utilized as a carbon source, but as a nitrogen source. Random amplified polymorphic DNA (RAPD) analysis indicated that the TNT induced no significant differences in microbial community composition and therefore, no major changes in natural selection, despite the increased microbial growth in the presence of the compound. More than 95% of the added TNT bound irreversibly to the sediments, possibly as a result of microbial transformation to reactive metabolites that subsequently bound covalently to components of the sediment. The results, taken together, suggest that no permanent change in the microbial ecology occurred as a result of the TNT amendment. This was probably due partly to the transient exposure of the microbial communities to the TNT before it became irreversibly bound to the sediment, and partly to the fact that TNT was not a primary growth substrate that strongly affects natural selection. 相似文献
14.
Top-down impact of bacterivorous nematodes on the bacterial community structure: a microcosm study 总被引:3,自引:0,他引:3
De Mesel I Derycke S Moens T Van der Gucht K Vincx M Swings J 《Environmental microbiology》2004,6(7):733-744
The influence of bacterivorous nematodes (Diplolaimelloides meyli, Diplolaimelloides oschei, Diplolaimella dievengatensis, Panagrolaimus paetzoldi) on the development of a bacterial community growing on decaying cordgrass detritus was studied in laboratory microcosm experiments. Cordgrass leaves were incubated on a sediment surface with a natural bacterial mixture containing bacteria from sediment, cordgrass detritus and habitat water. The four nematode species were applied separately to the microcosms; controls remained without nematodes. Samples were taken seven times over a 65-day period. The bacterial community structure was analysed by means of DGGE of the 16S rRNA genes. Multi Dimensional Scaling showed grouping of the samples per treatment. Analysis of Similarities indicated that the differences between treatments were significantly larger than differences within treatments. Our results suggest that nematodes can have a significant structuring top-down influence on the 'pool' of bacteria growing on the detritus, even at low densities. Dissimilarities were similar between all treatments. Differences in bacterial community composition within the treatments with monhysterids (D. meyli, D. oschei and D. dievengatensis) can be explained by species-specific food preferences. Panagrolaimus paetzoldi on the other hand feeds unselectively, and thus affects the bacterial community differently. A top-down effect of the nematodes on the diversity of the bacterial community was only evident under high grazing pressure, i.e. in the presence of P. paetzoldi. 相似文献
15.
Determining the identity and roles of oil-metabolizing marine bacteria from the Thames estuary, UK 总被引:6,自引:0,他引:6
Crude oil is a complex mixture of different hydrocarbons. While diverse bacterial communities can degrade oil, the specific roles of individual members within such communities remain unclear. To identify the key bacterial taxa involved in aerobic degradation of specific hydrocarbons, microcosm experiments were established using seawater from Stanford le Hope, Thames estuary, UK, adjacent to a major oil refinery. In all microcosms, hydrocarbon degradation was significant within 10 weeks, ranging from > 99% of low-molecular-weight alkanes (C(10)-C(18)), 41-84% of high-molecular-weight alkanes (C(20)-C(32)) and pristane, and 32-88% of polycyclic aromatic hydrocarbons (PAHs). Analysis of 16S rRNA sequences from clone libraries and denaturing gradient gel electrophoresis (DGGE) indicated that, except when incubated with fluorene, PAH-degrading communities were dominated by Cycloclasticus. Moreover, PAH-degrading communities were distinct from those in microcosms containing alkanes. Degradation of the branched alkane, pristane, was carried out almost exclusively by Alcanivorax. Bacteria related to Thalassolituus oleivorans (99-100% identity) were the dominant known alkane degraders in n-alkane (C(12)-C(32)) microcosms, while Roseobacter-related bacteria were also consistently found in these microcosms. However, in contrast to previous studies, Thalassolituus, rather than Alcanivorax, was dominant in crude oil-enriched microcosms. The communities in n-decane microcosms differed from those in microcosms supplemented with less volatile alkanes, with a phylogenetically distinct species of Thalassolituus out-competing T. oleivorans. These data suggest that the diversity and importance of the genus Thalassolituus is greater than previously established. Overall, these experiments demonstrate how degradation of different petroleum hydrocarbons is partitioned between different bacterial taxa, which together as a community can remediate petroleum hydrocarbon-impacted estuarine environments. 相似文献
16.
Magalie Stauffert Cristiana Cravo-Laureau Ronan Jézéquel Sandra Barantal Philippe Cuny Franck Gilbert Christine Cagnon Cécile Militon David Amouroux Fatima Mahdaoui Brice Bouyssiere Georges Stora Fran?ois-Xavier Merlin Robert Duran 《PloS one》2013,8(6)
Oil spills threaten coastlines where biological processes supply essential ecosystem services. Therefore, it is crucial to understand how oil influences the microbial communities in sediments that play key roles in ecosystem functioning. Ecosystems such as sediments are characterized by intensive bioturbation due to burrowing macrofauna that may modify the microbial metabolisms. It is thus essential to consider the bioturbation when determining the impact of oil on microbial communities. In this study, an experimental laboratory device maintaining pristine collected mudflat sediments in microcosms closer to true environmental conditions – with tidal cycles and natural seawater – was used to simulate an oil spill under bioturbation conditions. Different conditions were applied to the microcosms including an addition of: standardized oil (Blend Arabian Light crude oil, 25.6 mg.g−1 wet sediment), the common burrowing organism Hediste (Nereis) diversicolor and both the oil and H. diversicolor. The addition of H. diversicolor and its associated bioturbation did not affect the removal of petroleum hydrocarbons. After 270 days, 60% of hydrocarbons had been removed in all microcosms irrespective of the H. diversicolor addition. However, 16S-rRNA gene and 16S-cDNA T-RFLP and RT-PCR-amplicon libraries analysis showed an effect of the condition on the bacterial community structure, composition, and dynamics, supported by PerMANOVA analysis. The 16S-cDNA libraries from microcosms where H. diversicolor was added (oiled and un-oiled) showed a marked dominance of sequences related to Gammaproteobacteria. However, in the oiled-library sequences associated to Deltaproteobacteria and Bacteroidetes were also highly represented. The 16S-cDNA libraries from oiled-microcosms (with and without H. diversicolor addition) revealed two distinct microbial communities characterized by different phylotypes associated to known hydrocarbonoclastic bacteria and dominated by Gammaproteobacteria and Deltaproteobacteria. In the oiled-microcosms, the addition of H. diversicolor reduced the phylotype-richness, sequences associated to Actinobacteria, Firmicutes and Plantomycetes were not detected. These observations highlight the influence of the bioturbation on the bacterial community structure without affecting the biodegradation capacities. 相似文献
17.
Methyl ethyl ketone (MEK) is a common groundwater contaminant often present with more toxic compounds of primary interest. Because of this, few studies have been performed to determine the effect of microbial community structure on MEK biodegradation rates in aquifer sediments. Here, microcosms were prepared with aquifer sediments containing MEK following a massive spill event and compared to laboratory-spiked sediments, with MEK biodegradation rates quantified under mixed aerobic/anaerobic conditions. Biodegradation was achieved in MEK-contaminated site sediment microcosms at about half of the solubility (356 mg/L) with largely Firmicutes population under iron-reducing conditions. MEK was biodegraded at a higher rate [4.0 ± 0.74 mg/(L days)] in previously exposed site samples compared to previously uncontaminated sediments [0.51 ± 0.14 mg/(L days)]. Amplicon sequencing and denaturing gradient gel electrophoresis of 16S rRNA genes were combined to understand the relationship between contamination levels, biodegradation, and community structure across the plume. More heavily contaminated sediments collected from an MEK-contaminated field site had the most similar communities than less contaminated sediments from the same site despite differences in sediment texture. The more diverse microbial community observed in the laboratory-spiked sediments reduced MEK concentration 47 % over 92 days. Results of this study suggest lower rates of MEK biodegradation in iron-reducing aquifer sediments than previously reported for methanogenic conditions and biodegradation rates comparable to previously reported nitrate- and sulfate-reducing conditions. 相似文献
18.
Laminated Eocene maar-lake sediments from Eckfeld (Eifel region, Germany) and their short-term periodicities 总被引:1,自引:0,他引:1
J. Mingram 《Palaeogeography, Palaeoclimatology, Palaeoecology》1998,140(1-4):289-305
The Eocene age Eckfeld/Eifel maar lake is interesting both because of the excellently preserved fossil remains and also for the laminated sediment sequence deposited under subtropical conditions. The latitude, however, has a higher seasonal insolation difference than comparable recent environments due to the northward shift of climatic zones during the Eocene. The overall aim of this investigation was to compare sediment types and lake development of a Tertiary (subtropical) maar lake with recent maar lakes, to discuss the origin of laminations, and search for their possible periodicities. The main sediment types, which were exposed during recent paleontological excavations, are laminated oilshales, partly laminated bituminous siltstones, and graded beds (mostly turbidites), which sometimes include laminated clay tops. Some deeper parts of the profile drilled in 1980, are dominated by diatomaceous laminites. The amount of organic carbon (around 8.5%) is comparable with recent lakes with low sedimentation rates. The high C/N ratios (25–30) of the Eckfeld sediments, as well as the fact that the highest Corg values are found in the graded beds, shows the importance of terrestrial plant debris for these lake sediments. Lamination of the oil shales was caused by the complex interrelationship of clastic supply (mineral and plant detritus), autochthonous organic deposition (mainly green algae), and early diagenetic siderite formation. The darker sublaminae represent the coarser clastic (organic and inorganic) input together with the autochthonous supply from the lake (green algae), whereas the lighter sublaminae are composed of settled fine-grained, mostly siliciclastic detritus derived from the probable yearly turnover of the density stratification of the lake. The thickness of the fine siliciclastic and organo-clastic layers, the morphology and palaeogeographical situation of the maar lake, and the occurrence of algal layers, strongly suggest that the laminated sediments are varves (although there is no definitive proof of this). Thickness variations from ca. 700 oil shale laminae were measured and computed. Time series analysis yielded at least one certain period of 5.5 yr, accepting that the laminae are indeed varves. The duration of the laminated lake sequence from Eckfeld maar, as deduced from sedimentation rates of the different sediment types is ca. 82,000 yr and, thus, within the typical life time of other small maar lakes. 相似文献
19.
The biodegradation of tert-butylphenyl diphenyl phosphate (BPDP) was examined in microcosms containing sediment and water from five different ecosystems as part of our studies to elucidate the environmental fate of phosphate ester flame retardants. Biodegradation of [14C]BPDP was monitored in the environmental microcosms by measuring the evolution of 14CO2. Over 37% of BPDP was mineralized after 8 weeks in microcosms from an ecosystem which had chronic exposure to agricultural chemicals. In contrast, only 1.7% of BPDP was degraded to 14CO2 in samples collected from a noncontaminated site. The exposure concentration of BPDP affected the percentage which was degraded to 14CO2 in microcosms from the two most active ecosystems. Mineralization was highest at a concentration of 0.1 mg of BPDP and was inhibited with 10- and 100-fold higher concentrations of BPDP in these microcosms. Indigenous heterotrophic and BPDP-utilizing microbial populations and phosphoesterase enzyme activities were highest in sediments which had the highest biodegradation of BPDP. We observed adaptive increases in both microbial populations and phosphoesterase enzymes in some sediments acclimated to BPDP. Chemical analyses of the residues in the microcosms indicated undegraded BPDP and minor amounts of phenol, tert-butylphenol, diphenyl phosphate, and triphenyl phosphate as biodegradation products. These data suggest that the microbial degradation of BPDP results from at least three catabolic processes and is highest when low concentrations of BPDP are exposed to sediment microorganisms of eutrophic ecosystems which have high phosphotri- and diesterase activities and previous exposure to anthropogenic chemicals. 相似文献
20.
The effects of naphthalene on microbial communities in the bottom boundary layer of the Delaware Bay estuary were investigated in microcosms using denaturing gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH) with oligonucleotide probes. Three days after the addition of naphthalene, rates of bacterial production and naphthalene mineralization were higher than in no-addition controls and than in cases where glucose was added. Analyses using both DGGE and FISH indicated that the bacterial community changed in response to the addition of naphthalene. FISH data indicated that a few major phylogenetic groups increased in response to the glucose addition and especially to the naphthalene addition. DGGE also demonstrated differences in community composition among treatments, with four phylotypes being unique to naphthalene-amended treatments and three of these having 16S rRNA genes similar to known hydrocarbon degraders. The bacterial community in the naphthalene-amended treatment was distinct from the communities in the glucose-amended treatment and in the no-addition control. These data suggest that polycyclic aromatic hydrocarbons may have large effects on microbial community structure in estuaries and probably on microbially mediated biogeochemical processes. 相似文献