首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 46 毫秒
1.
Summary This study was conducted to assess the genetic basis of the variability observed for the glutamate oxaloacetate transaminase (GOT), Superoxide dismutase (SOD), esterase (EST), and malate dehydrogenase (MDH) isozyme systems in different open-pollinated Vicia faba varieties. Individual plants showing contrasting zymogram patterns were simultaneously selfed and cross-combined. Crossing was unsuccessful in producing progeny, and only selfed progenies were suitable for genetical analysis of isozyme variability. Three zones of GOT activity were made visible. The isozyme of GOT-2 and GOT-3 zones were dimeric and under the control of three alleles at the Got-2 locus and two alleles at the Got-3 locus, respectively. The isozymes of the GOT-1 zone did not show any variability. Three zones of SOD isozyme activity were made visible. The isozymes occurring in the SOD-1 (chloroplastic isozyme form) and SOD-2 (cytosol isozyme form) zones were dimeric and under the control of two alleles at the Sod-1 and Sod-2 loci. The isozyme visualized in the SOD-3 zone (mitochondrial isozyme form) were tetrameric and under the control of two alleles at the Sod-3 locus. Apparently the isozymes made visible in the most anodal esterase zones EST-1, EST-2, and EST-3 were monomeric, and the occurrence of two alleles at each of two different loci explained the variability observed in the EST-2 and EST-3 zones. For MDH, only two five-banded zymogram pattern types were found, and every selfed progeny showed only one of the two zymogram type, indicating that each individual possessed fixed alleles at the loci controlling MDH isozyme. Got-2, Got-3, Sod-1, Sod-2, and Sod-3 appear to be five new isozyme gene markers that can be useful in Vicia faba breeding for linkage study, varietal fingerprinting, outcrossing rate estimate, and indirect selection for quantitative characters.  相似文献   

2.
Summary The genetics of peroxidase and leucine-aminopeptidase isozymes was studied utilizing starch gel electrophoresis in the diploidsMedicago sativa L. (M. coerulea Less.) andM. falcata L. Three anodal and one cathodal sets of peroxidase isozymes identify four linked loci. In addition, two anodal sets of leucine-aminopeptidase isozymes identify two loci that may be linked. The allozymes at each of the loci segregated as expected for monomeric enzymes. However in several crosses there were deficiencies in the number of progeny in particular genotypic classes. This could result from the segregation of recessive deleterious genes linked to some of the allozyme alleles. This is the first report of multiple loci and multiple alleles determining isozymes inMedicago. Supported by grants from the Alberta Research Council (No. D1B02 to R.C. von Borstel) and the Computer Use and Policy Committee, University of Alberta  相似文献   

3.
The LDH isozymes were surveyed in bacterized cultures of syngens 1, 3, 12, and 13 of Paramecium caudatum by polyacrylamide gel electrophoresis. All the examined stocks of different syngens except one stock in syngen 3 had a single common LDH isozyme, and intra- and intersyngenic variation was not observed except for the one stock in syngen 3. Breeding data using the exceptional stock indicated that the LDH isozymes of P. caudatum are controlled by two codominant alleles at a single locus whose products aggregate randomly, forming a dimer.  相似文献   

4.
Fusarium head blight (FHB) is a devastating disease in wheat that reduces grain yield, grain quality and contaminates the harvest with deoxynivalenol (DON). As potent resistance sources Sumai 3 and its descendants from China and Frontana from Brazil had been analysed by quantitative trait loci (QTL) mapping. We introgressed and stacked two donor QTL from CM82036 (Sumai 3/Thornbird) located on chromosomes 3B and 5A and one donor QTL from Frontana on chromosome 3A in elite European spring wheat and estimated the effects of the three individual donor QTL and their four combinations on DON, Fusarium exoantigen content, and FHB rating adjusted to heading date. One class with the susceptible QTL alleles served as control. Each of the eight QTL classes was represented by 12–15 F3-derived lines tested in F5 generation as bulked progeny possessing the respective marker alleles homozygously. Traits were evaluated in a field experiment across four locations with spray inoculation of Fusarium culmorum. All three individual donor-QTL alleles significantly reduced DON content and FHB severity compared to the marker class with no donor QTL. The only exception was the donor-QTL allele 3A that had a low, but non-significant effect on FHB severity. The highest effect had the stacked donor-QTL alleles 3B and 5A for both traits. They jointly reduced DON content by 78% and FHB rating by 55% compared to the susceptible QTL class. Analysis of Fusarium exoantigen content illustrates that lower disease severity is associated with less mycelium content in the grain. In conclusion, QTL from non-adapted sources could be verified in a genetic background of German elite spring wheat. Within the QTL classes significant (P<0.05) genotypic differences were found among the individual genotypes. An additional phenotypic selection would, therefore, be advantageous after performing a marker-based selection.  相似文献   

5.
Allozyme variation in mixed populations of Mielichhoferia elongata and M. mielichhoferiana was investigated to determine if interspecific hybridization occurs when these two closely related species grow together. Previous research has shown that M. elongata and M. mielichhoferiana can be distinguished by three diagnostic isozyme loci (Gpi-1, Mdh-2, and Mdh-3) at which the two species do not share alleles in 32 allopatric populations from North America and Europe. The present study shows that in five populations from Colorado, Norway, and Sweden, gametophytes resulting from interspecific hybridization can be recognized by recombinant genotypes combining alleles of the otherwise diagnostic loci. A total of 32 multilocus genotypes was found among the 111 individuals sampled, of which 13 were recombinants. The frequency of recombinants ranged from 12% to 35% within populations, and all but one population contained both parental species. Moreover, recombinant genotypes could be accounted for by the allelic constitution of sympatric parents. In two of the populations, more than one hybridization event was necessary to account for the diversity of recombinant genotypes. Twenty-nine of the 32 genotypes detected in this study were restricted to one population each, two occurred in two Swedish populations separated by approximately 14 km, and one occurred in both Sweden and Norway.  相似文献   

6.
Summary The extent of correlation was estimated between isozyme genotypes and the four widely segregating characters — leaf segment W/L ratio, stigma exsertion, fruit weight, and seed weight — in the first backcross of F1 Lycopersicon esculentum x Solanum pennellii to the former parent. The inbred parents differ in their alleles at the 12 tested isozymic loci, which are known to mark a minimum of eight of the twelve tomato chromosomes. Based on the isozyme data, a mean heterozygosity value, ¯H, was calculated which estimates the proportion of pennillii alleles in each individual. Correlations between mean heterozygosity and observed levels of each quantitative trait were highly significant and positive or negative as expected from the relative parental values. Plants with the lowest mean heterozygosity — i.e., closest to the esculentum zymotype also had mean values closest to those of this parent amongst the whole backcross population for each of the quantitative traits.Bivariate and multiple regression analysis was used to evaluate the ability of isozymes vs diagnostic morphological characters to estimate the portion of recurrent parent genes carried in each backcross individual. The results suggest that isozyme data gives better estimates than single diagnostic morphological characters and approach the level obtained by combinations of three morphological traits. Since electrophoretic determinations are made on small seedlings, selection at that stage can effect great savings of space and effort by greatly deminishing the size of the population needed at maturity. As such, isozyme selection would precede morphological selection but not replace it, thus the predictive value of these biochemical markers as well as diagnostic morphological characters could be obtained.  相似文献   

7.
Summary The inheritance of 6-phosphogluconate dehydrogenase (6PGD), malate dehydrogenase (MHD), aconitase (ACO), phosphoglucomutase (PGM), phosphoglucoisomerase (PGI), and glutamate-oxalacetate transaminase (GOT) polymorphic isozymes was studied in leaf extracts of nine hazelnut progenies using horizontal starch gel electrophoresis. Evidence of Mendelian inheritance was obtained for ten loci: 6-Pgd-2, Mdh-1, Aco-1, Aco-2, Pgm-1, Pgm-2, Pgm-3, Pgi-2, Pgi-3, and Got-2, which permitted the analysis of 28 alleles (2.8 per locus). The presence of null alleles was detected in Pgm-1 and Pgm-3. Joint segregation analysis of pairs of isozymes revealed four linkages: Mdh-1-Pgi-2, Aco-2-Pgm-2, Pgm-1-Pgm-3, and 6Pdg-2-Pgm-2.  相似文献   

8.
Recombination at the Rp1 locus of maize.   总被引:11,自引:0,他引:11  
Summary The Rp1 locus of maize determines resistance to races of the maize rust fungus (Puccinia sorghi). Restriction fragment length polymorphism markers that closely flank Rp1 were mapped and used to study the genetic fine structure and role of recombination in the instability of this locus. Susceptible progeny, lacking the resistance of either parent, were obtained from test cross progeny of several Rp1 heterozygotes. These susceptible progeny usually had non-parental genotypes at flanking marker loci, thereby verifying their recombinational origin. Seven of eight Rp1 alleles (or genes) studied were clustered within about 0.2 map units of each other. Rpl G, however, mapped from 1–3 map units distal to other Rp1 alleles. Rp5 also mapped distally to most Rp1 alleles. Other aspects of recombination at Rp1 suggested that some alleles carry duplicated sequences, that mispairing can occur, and that unequal crossing-over may be a common phenomenon in this region; susceptible progeny from an Rp1 A homozygote had recombinant flanking marker genotypes, and susceptible progeny from an Rp1 DlRp1 F heterozygote showed both possible nonparental flanking marker genotypes.  相似文献   

9.

Key message

We identified 27 stable loci associated with agronomic traits in spring wheat using genome-wide association analysis, some of which confirmed previously reported studies. GWAS peaks identified in regions where no QTL for grain yield per se has been mapped to date, provide new opportunities for gene discovery and creation of new cultivars with desirable alleles for improving yield and yield stability in wheat.

Abstract

We undertook large-scale genetic analysis to determine marker-trait associations (MTAs) underlying agronomic and physiological performance in spring wheat using genome-wide association studies (GWAS). Field trials were conducted at seven sites in three countries (Sudan, Egypt, and Syria) over 2–3 years in each country. Twenty-five agronomic and physiological traits were measured on 188 wheat genotypes. After correcting for population structure and relatedness, a total of 245 MTAs distributed over 66 loci were associated with agronomic traits in individual and mean performance across environments respectively; some of which confirmed previously reported loci. Of these, 27 loci were significantly associated with days to heading, thousand kernel weight, grain yield, spike length, and leaf rolling for mean performance across environments. Despite strong QTL by environment interactions, eight of the loci on chromosomes 1A, 1D, 5A, 5D, 6B, 7A, and 7B had pleiotropic effects on days to heading and yield components (TKW, SM?2, and SNS). The winter-type alleles at the homoeologous VRN1 loci significantly increased days to heading and grain yield in optimal environments, but decreased grain yield in heat prone environments. Top 20 high-yielding genotypes, ranked by additive main effects and multiplicative interaction (AMMI), had low kinship relationship and possessed 4–5 favorable alleles for GY MTAs except two genotypes, Shadi-4 and Qafzah-11/Bashiq-1–2. This indicated different yield stability mechanisms due to potentially favorable rare alleles that are uncharacterized. Our results will enable wheat breeders to effectively introgress several desirable alleles into locally adapted germplasm in developing wheat varieties with high yield stability and enhanced heat tolerance.
  相似文献   

10.
The isozymes of alcohol dehydrogenase (ADH; E.C. 1.1.1.1) in wild and cultivated sunflower (Helianthus annuus) seeds can be resolved electrophoretically into 12 bands. The slowest- and probably the fastest-migrating sets of three are allozymic products of two genes, Adh 1 and Adh 2 , each having two alleles, F (for fast) and S (for slow). Evidence from dissociation-recombination experiments utilizing bands excised from starch gels indicates that an intermediately-migrating isozyme is a dimeric intergenic product consisting of ADH-1F and ADH-2S subunits. The hybrid isozyme was unstable in vitro in that its monomers spontaneously dissociated and recombined to produce ADH-1FF and ADH-2SS isozymes. The molecular weights of the hybrid as well as the parental isozymes were estimated at approximately 98,000.Supported by a Graduate School Research grant of the University of Kansas and by NSF grant GB-35853.  相似文献   

11.
Honckenya peploides is a subdioecious dune plant that reproduces both sexually and by clonal growth. In northwest Spain this species was found to exhibit an extreme spatial segregation of the sexes, and our objective was to investigate genetic variation in unisexual clumps. Genetic variation was studied in six unisexual clumps of H. peploides, three of them exclusively composed of males and three exclusively female. In total, 193 samples were analysed using isozyme analysis and 80 samples were analysed using two AFLP primer combinations. Both techniques revealed considerably high genetic diversity (average proportion of distinguishable genotypes: 0.22 for isozymes and 0.36 for AFLP; average Simpson’s D: 0.65 for isozymes and 0.68 for AFLP). Our results show that, in spite of clonal growth, each unisexual clump consists of different genotypes. Genetic diversity within clumps is similar for both sexual morphs. Reasons for unisexuality of the clumps are discussed.  相似文献   

12.
The effect of GA3 on coleoptile-and first leaf elongation of tall (rht1) and semi-dwarf (Rht1) nearly-isogenic genotypes, within each of 25 random F9 wheat families, was determined on seedlings grown in a growth room at 18 °C. Conspicuous and very significant inter-family variation in the response of the first leaf to GA3 application was found in both the rht1 and Rht1 genotypes. The magnitudes of the response of the different families within genotypes to GA3 were not related to the leaf length of their untreated seedlings. It is suggested that, under given environmental conditions, background genotypic effects, inducing inter-family variation in responsiveness to GA3, regulate the elongation growth up to the limits set by the Rht alleles.  相似文献   

13.
Summary Forty-one hexaploid wheat genotypes have been examined for RFLPs detected by a -amylase probe using three restriction enzymes, and for mature grain -amylase isozyme polymorphism following IEF. The two homoeoallelic series assayed for RFLPs differed: little variation was found at group 2 chromosome homoeoloci, while the group 4/5 chromosome homoeoloci displayed considerable variation. Varieties that displayed a RFLP with one RE almost always did likewise with the other two REs, suggesting that most of the polymorphisms observed were due to large DNA rearrangements. Comparison of the variation in grain -amylase isozymes with the RFLP results indicated strong associations between particular RFLP and isozyme alleles.  相似文献   

14.
Wheat pre-harvest sprouting (PHS) is an undesired trait, which often reduces yield and downgrades end-use quality of grain. Viviparous-1B (Vp-1B), a regulator gene located on chromosome 3B, has previously been proved to be involved in inducing grain dormancy of wheat. In order to obtain some new or useful alleles associated with PHS tolerance of white-grained wheat, we developed a gene-specific marker (Vp1-b2) to identify allelic variations of Vp-1B using denaturing PAGE in micro-core collections of Chinese wheat and landraces. As a main component observed genetic variation for PHS, seed dormancy evaluated by germination index (GI) was determined at dough-yellow ripening stage in the present study. The results indicated that six alleles of Vp-1B, in our study, were discovered among 276 Chinese wheat varieties. Of these alleles, two variants were validated to be novel alleles and designated as Vp-1Be and Vp-1Bf, respectively. By investigating the association between allelic variations of Vp-1B and seed dormancy, we found allele of Vp-1Ba always inclined to weak seed dormancy and susceptibility to PHS. Up to 62.2% genotypes carrying the allele had high GI value with a range of 0.51–1.00, only 14.4% genotypes had low GI value under 0.30. On the contrary, other variants such as Vp-1Bb, Vp-1Bc, Vp-1Bd, Vp-1Be and Vp-1Bf mostly occurred in varieties with higher PHS tolerance, which average of GI values were 0.204, 0.227, 0.296, 0.256 and 0.186, respectively. In Chinese wheat germplasms, Vp-1Ba and Vp-1Bc showed the most widespread distribution followed by Vp-1Bb; other alleles fell into less used varieties. Our research confirmed rich allelic variation of Vp-1B occurred in micro-core collections of Chinese wheat and landraces, which may be useful for improving PHS tolerance as breeding parents.  相似文献   

15.
Polymorphism of the BoLA-DRB3 gene was studied with the use of the PCR-RFLP technique in three cattle breeds (Mongolian, Kalmyk, and Yakut) representing the Bos taurus turano-mongolicus group. 35 BoLA-DRB3.2 alleles were detected in the Mongolian breed and 34 alleles in the Kalmyk breed. The frequencies of alleles in both populations are distributed rather evenly: the frequencies of the most widely represented alleles (*18, *20, and *28) in the Mongolian cattle varied from 7.75 to 8.45%. The most frequent alleles in the Kalmyk cattle were *28 (14.52%), *24 (7.26%), and *12 (6.45%). Only five alleles were identified in the Yakut cattle breed. The prevailing allele was *29 (77.3%); a relatively frequent allele was *1 (13.1%), and the remaining three alleles constituted only 9.6%. Such a low level of diversity of BoLA-DRB3 gene alleles was not observed earlier in any other cattle breed. The Mongolian and Kalmyk breeds showed a wide diversity of BoLA-DRB3 genotypes (56 and 51 genotypes, respectively) and a high level of expected heterozygosity (H e = 0.953 and 0.946, respectively). Both breeds had a deficiency of heterozygotes (Mongolian cattle: H o = 0.775, D = −0.187; Kalmyk cattle: H o = 0.708, D = −0.252). A low level of genotypic diversity for the BoLA-DRB3 locus (only seven genotypes; the frequency for the genotype *29/*29 is 71.4%) and a very low level of observed heterozygosity (H o = 0.12) were revealed in the Yakut breed. BoLA-DRB3.2 alleles associated with resistance to persistent lymphocytosis caused by the bovine leukemia virus (total frequencies 15.49 and 24.19%) and to various forms of mastitis (total frequencies 12.68 and 20.96%, respectively) were identified in the Mongolian and Kalmyk animals. In the Yakut breed, alleles associated with resistance to diseases are represented only by the BoLA-DRB3.2 allele *7 (1.2%). Thus, the Mongolian and Kalmyk cattle breeds are characterized by a wide diversity of alleles and genotypes for the BoLA-DRB3 gene. In contrast, the population of Yakut cattle from the Verkhoyanskii region of the Republic of Sakha has a poor diversity of alleles and genotypes for the BoLA-DRB3 gene and a very low level of heterozygosity, suggesting an unfavorable state of the population that is probably caused by inbreeding depression due to a long-term isolation and a small number of animals.  相似文献   

16.
Summary A distorted segregation of esterase alleles at the complex loci, Est1, Est2 and Est4, was found in an F2 population. This distortion is typical for cross combinations between the Ga2Ga2 and ga2ga2 genotypes responsible for segregation distortion, since the Ga2 locus is linked with the complex loci encoding the esterase isozymes. The segregation of esterase isozyme patterns in F2 populations between 473 varieties of barley and a tester of ga2ga2 genotype was examined, and the genotypes inducing segregation distortion were detected. Varieties with a ga2ga2 genotype are widely distributed throughout the world, whereas Ga2Ga2 varieties are found only in eastern and southern regions of Asia, from Japan to North India, with a low frequency. In varieties collected from these regions, some associations were detected between alleles at the Ga2 locus and esterase isozyme patterns. Additionally, most of the Ga2 barley varieties are naked and possess a BtBtbt2bt2 genotype for a non-brittle rachis.  相似文献   

17.
P. Eggleston 《Genetica》1987,72(3):181-186
A range of long established inbred lines derived from the TEXAS population of Drosophila melanogaster have been used to elucidate the nature of the competitive interactions which occur in genetically heterogeneous mixtures. A prerequisite for this type of investigation is the ability to distinguish the genotypes which compete in mixed culture. Specific marker alleles are generally used to achieve this distinction although in the past little attention has been given to the possibility of competitive bias introduced by the marker alleles themselves. For the experiments reported in this paper two specific marker alleles (y 2 and w a) have been introduced independently into the TEXAS inbred lines. In this way the original wild type inbred lines could be compared with similar series of genotypes marked with either y 2 or w a and the effects of the marker alleles determined.The results indicated that the body colour mutation (y 2) was neutral in its effect on the competitive interaction of recipient strains. The introduction of the white apricot eye colour mutation (w a) however, had a pronounced and deleterious effect on competitive ability. This effect was to render genotypes less able to compete effectively in mixed culture by depressing inter-genotypic competitive ability. These effects were found to be consistent over a range of genotypes and for each of two characters measuring competitive success.  相似文献   

18.
Summary The genetic control of hexokinase isozymes (ATP: d-hexose-6-phosphotransferase, E.C. 2.7.7.1, HEX) in maize (Zea mays L.) was studied by starch gel electrophoresis. Genetic analysis of a large number of inbred lines and crosses indicates that the major isozymes observed are encoded by two nuclear loci, designated Hex1 and Hex2. Five active allozymes and one null variant are associated with Hex1, while Hex2 has nine active alleles in addition to a null variant. Alleles at both loci govern the presence of single bands, with no intragenic or intergenic heteromers visible, suggesting that maize HEX's are active as monomers. Organelle preparations demonstrate that the products of both loci are cytosolic. All alleles, including the nulls, segregate normally in crosses. Vigorous and fertile plants were synthesized that were homozygous for null alleles at both loci, suggesting that other hexosephosphorylating enzymes exist in maize that are undetected with our assay conditions. Linkage analyses and crosses with B-A translocation stocks place Hex1 on the short arm of chromosome 3, 27 centimorgans from Pgd2 (phosphogluconate dehydrogenase) and Hex2 on the long arm of chromosome 6, approximately 45 centimorgans from Pgd1. It is suggested that the parallel linkages among these two pairs of duplicated genes reflects an evolutionary history involving chromosome segment duplication or polyploidy.Paper No. 10170 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC  相似文献   

19.
Summary The data on number of grains/spike, 100 grain weight and grain yield/plant in eighteen genotypes of four genome combinations (AABB- 4 genotypes, AABBDD- 6 genotypes, AABBRR- 5 genotypes and AABBDDRR- 3 genotypes) were recorded for eight environments created by combining two dates of sowing, two fertilizer regimes and two spacings. Two stability parameters-regression coefficient (b) and deviation from regression (Sd 2) were computed. Joint regression analyses revealed that the genotypes differed significantly for these characters. A significant variation due to environments was also found. A comparative study of performance of genotypes belonging to four genome combinations revealed that the genes for stability are not uniformly distributed in these genome combinations. Stability may largely depend on gene combination rather than on genome combination.  相似文献   

20.
H. Qavi  S. Kit 《Biochemical genetics》1980,18(7-8):669-679
A histochemical procedure has been developed for staining aminoacylase-1 (ACY-1) isozymes after electrophoresis on cellulose acetate membranes. N-Formyl-L-methionine and N-acetyl-L-methionine were excellent enzyme substrates in the staining reaction. The ACY-1 isozymes from tissue culture cells of several vertebrate species showed distinguishable electrophoretic patterns. The ACY-1 isozymes in extracts of mouse, human, owl monkey, and African green monkey kidney cells each had electrophoretic mobilities different from those of peptidases S, A, and C from the same cells. Except for African green monkey kidney (CV-1) cells, the animal species expressed a single anodally migrating ACY-1 band. Human-mouse somatic cell hybrids containing the short arm of human chromosome 3 expressed three ACY-1 bands, as would be predicted from the dimeric structure of the enzyme. CV-1 cells expressed two (or three) ACY-1 bands, suggesting the possibility that CV-1 cells contained two alleles at a single locus or two genetic loci for ACY-1.This research was aided by USPHS Grants CA-06656-17 and 1-K6KAI-2352-17 from the National Cancer Institute and the National Institute of Allergy and Infectious Diseases, and by Contract NO1-CP-71058 from the National Cancer Institute.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号