FURTHER STUDIES ON MOBILIZATION OF CFUs

Mobilization of CFUs from haemopoietic tissues into circulation was studied after injection of different bacterial lipopolysaccharides (LPS), zymosan, phytohaemagglutinin (PHA), concanavalin A (Con A), trypsin and di-isopropyl-fluorophosphate-inhibited trypsin. All bacterial LPS used gave an increase of CFUs in the peripheral blood at 1 h after i.v. injection. Some variation in activity could not be excluded. As with Salmonella typhosa LPS, zymosan gave an increase in circulating CFUs during the first few hr and a second peak a few days later. After injection of zymosan as well as S. typhosa LPS the second peak in the blood was accompanied by a large increase in CFUs numbers in the spleen. PHA gave an immediate mobilization of CFUs, but the mobilization after injection of Con A during the first few hr occurred more slowly. After injection of S. typhosa LPS, zymosan and PHA the blood C3 level was found to be depressed considerably. This might indicate that the complement system is involved in the early mobilization of CFUs. Dexamethasone, a synthetic hormone which has been reported to give sequestration of several cell types in the bone marrow, did not inhibit the early and late mobilization of CFUs which normally occurs after injection of S. typhosa LPS.     

红细胞抗高血压因子降压作用的进一步研究

我们曾报道原发性高血压患者(EHS)红细胞抗高血压因子(AHF)具有缓慢而持久的降压作用。本工作表明,AHF对卒中易感型自发性高血压大鼠(SHRsp)还具有快速短暂的降压作用,注射AHF后10—30s,SHRsp收缩压从原水平的26.8±1.7kPa降至20.1±1.5kPa(P<0.001)。正常人和大鼠红细胞AHF的降压作用明显强于EHS和高血压大鼠AHF。此外我们还发现EHS血浆中存在升压物质。以上结果提示,AHF缺乏和升压物质含量相对较高可能是原发性高血压发病的一个重要原因。     

FURTHER STUDIES ON THE INDUCTION OF THE DRUG-HYDROXYLATING ENZYME SYSTEM OF LIVER MICROSOMES

Further studies of the induction of the liver microsomal drug-hydroxylating enzyme system by pretreatment of rats with various drugs are presented. The phenobarbital-induced increase in the microsomal content of CO-binding pigment and in the activities of TPNH-cytochrome c reductase and the oxidative demethylation of aminopyrine is proportional, within certain limits, to the amount of phenobarbital injected. Removal of the inducer results in a parallel decrease in the levels of CO-binding pigment, TPNH-cytochrome c reductase, and aminopyrine demethylation. Other inducing drugs have been investigated and shown to act similarly to phenobarbital. The early increase in these enzymes is found in the microsomal subfraction consisting of rough-surfaced vesicles, whereas repeated administration of the inducing drug results in a concentration of the enzymes in the smooth-surfaced vesicles. The phenobarbital-stimulated formation of endoplasmic membranes is reflected in increased amounts of the various microsomal phospholipid fractions as revealed by thin layer chromatography. There is no significant difference between the stimulated rates of P_i³² incorporation into phospholipids of the two different microsomal subfractions in response to phenobarbital treatment. The drug-induced enzyme synthesis is unaffected by adrenalectomy.     

FURTHER STUDIES ON MEIOSIS IN THE MYXOMYCETES

Ross, Ian K. (Yale U., New Haven, Conn.) Further studies on meiosis in the Myxomycetes. Amer. Jour. Bot. 48(3): 244–248. Illus. 1961.—Photomicrographs of meiotic figures in 5 species of Myxomycetes are presented as further evidence of the time and location of meiosis in these organisms. Three members of the Physarales studied differed from species studied previously in that the cleavage of the protoplasm began during the second meiotic division and not after the divisions had been completed. Chromosome numbers were found to be in accord with previous observations and ranged from n = 25 to 90 depending on the species.     

BACTERIOLOGICAL STUDIES OF IMMERSION CLEANING OF MILKING EQUIPMENT ON COMMERCIAL FARMS

SUMMARY: A study has been made of the immersion cleaning technique as applied on commercial farms. In an initial survey bacteriological results for rinses and swabs of the milking equipment were satisfactory on most farms, but on one farm the results were very disappointing. Further investigations at this farm showed that the poor results were caused partly by sporing bacteria which survived the caustic soda treatment, presumably in the spore state, and partly by non-sporing organisms, chiefly chromobacteria and micrococci.
These non-sporing organisms are found in abundance on the surface of dirty wash troughs, and under certain conditions re-contamination of the milking equipment can occur during the rinsing operation. Suggested modifications to the original immersion cleaning technique include the use of hot water for the before-milking hypochlorite rinse, brushing the equipment in the after-milking rinse to remove extraneous dirt, regular cleansing of the wash trough and the monthly cleansing of the basket and bin.     

鲤脑促性腺激素释放激素分布的进一步研究

鱼类促性腺激素释放激素GRH核群与神经纤维的分布,潘家秀等1979曾报道11月份采集的卵巢处于Ⅳ期准备过冬的鲤鱼(Cyprinus carpio)颅腔经甲醇注射后下丘脑GRH的酶免疫标记定位结果:免疫活性GRH(ir-GRH)核群在视前区、视前隐窝区、前脑侧束区、下丘脑外侧区、视交叉等处均有ir-GRH纤维分布。本文报道的是2月份采集的鲤,即垂体促性腺激素GTH含量最丰富时(曹术超等1975),怀卵鲤脑及垂体ir-GRH的酶免疫标记。 结节外侧核(NLT)对于鱼类生殖功能有调节作用(Peter等,1978;Roi,1973),然而11月鲤鱼NLT未能染出ir—GRH。本文除采用另一季节的鲤鱼继续标染外,也试用抗     

FURTHER STUDIES ON THE RNA FUNCTIONAL IN AUXIN ACTION

Avena coleoptile and a respiration-deficient mutant of yeastwhose cells are elongated by the action of auxin in the absenceof GA added were subjected to the phenol method of RNA fractionation."RNA" from the phenol layer promoted the auxin-induced expansionof Jerusalem artichoke tuber slices just as GA did, whereas"RNA" from the water layer was ineffective. The GA-like activityof the former "RNA" was reduced by treatment with RNase or alkali. No active "RNA" was extractable from a respiration-sufficientyeast strain which does not respond to auxin without GA supplemented. (Received August 19, 1964; )     

氯氰菊酯对斜生栅藻的毒性研究

研究了农药氯氰菊酯对斜生栅藻的毒性效应,结果显示：以丙酮为溶剂量,其96h EC50为112.45mg/L;以乙酸乙酯为溶剂时,其96hEC50为112.81mg/L。同时也研究了氯氰菊酯对斜生栅藻的光合色素含量、色素光谱、可溶性蛋白质含量和超氧物歧化酶活性的影响。