共查询到20条相似文献,搜索用时 15 毫秒
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5' distal and proximal cis-acting regulator elements are required for developmental control of a rice seed storage protein glutelin gene 总被引:1,自引:0,他引:1
Zhenwei Zheng Yasushi Kawagoe Shouhua Xiao Zhijian Li Thomas Okita Toan Lam Hau Angy Lin Norimoto Murai 《The Plant journal : for cell and molecular biology》1993,4(2):357-366
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N M Lardy R M Bakas A R van der Horst E van Twuyver R E Bontrop L P de Waal 《Journal of immunology (Baltimore, Md. : 1950)》1992,148(8):2572-2577
Pedigree analysis of a Dutch family revealed the presence of an HLA-A "blanc" allele segregating with the HLA-B8, -Cw7, -DR3, -DR52, -DQ2 haplotype. Precipitation studies, using selected mAb and sera directed against conserved epitopes of HLA class I products, failed to detect the expression of a corresponding HLA-A locus product. cDNA nucleotide sequence analysis of the HLA-A "blanc" specificity showed that the obtained sequence was identical to the authentic HLA-A1 gene revealing no mutations, deletions or recombinations that could influence translation or transport of a putative translation product to the cell surface. Mitogen stimulation, EBV transformation, or treatment with rIFN-gamma and rTNF-alpha did not induce HLA-A1 expression. Furthermore, cell-mediated lympholysis analysis revealed that individuals carrying the nonexpressed HLA-A1 gene could mount a strong anti-HLA-A1 T cell response, indicating that HLA-A1 was not expressed during T cell ontogeny. Therefore, this study describes for the first time the allele-specific down-regulation of the expression of a classical HLA class I gene segregating in healthy individuals. 相似文献
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Y Y Zhao W Gu M A Siddiqui A Kumar 《Cellular and molecular biology, including cyto-enzymology》1992,38(1):71-80
Angiotensinogen is the precursor molecule of one of the most potent vasoactive substances, angiotensin-II. Angiotensinogen is normally synthesized in the liver and secreted into the plasma where it is converted into angiotensin-II by the combined proteolytic action of renin and angiotensin converting enzyme. Angiotensinogen levels in the plasma are modulated by a number of pathological and physiological factors. In order to understand the regulation of angiotensinogen gene expression, we have constructed an expression vector in which 688 bp of the 5'-flanking region of the rat angiotensinogen gene were attached to the chloramphenicol acetyl transferase (CAT) coding sequence. We have also obtained 5'-sequential deletion mutants from the rat angiotensinogen promoter attached to the CAT gene, and have identified multiple cis-acting DNA sequences involved in the regulation of angiotensinogen gene expression by transient transfection of these recombinant DNA molecules in human hepatoma cell lines, Hep3B, and HepG2. 相似文献
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