The mylohyoid bridge in four population samples from India, with observations on its suitability as a genetic marker

Four adult skeletal samples from the states of Uttar Pradesh, Andhra Pradesh, and Bihar in India have been studied for the incidence of mylohyoid bridge. The incidence, varying between 2.98% and 7.14%, has been compared with frequencies reported for other populations of the world. The range of variation for Indians, as a whole, falls within the lower levels of the spectrum of worldwide variation for this trait, ranging between 0.47% for French Europeans and 33.8% observed among Plains American Indians. While noting its possible significance for clinical purposes, the suitability of the mylohyoid bridge as a population genetic marker has been discussed. It is emphasized that its use as a genetic marker in isolation of other discrete traits has serious limitations. For meaningful population definition and relationships as many discrete variants as possible ought to be utilized.     

Nonmetric cranial variation and biological distances between samples of six nontribal populations of India

Sixty-nine nonmetrical morphological variants of the cranium have been studied in six samples of non-tribal, state populations in India, and their incidence reported. Using C.A.B. Smith’s angular transformation of frequencies, the multivariate Thetasquare distances and their respective standard deviations have been presented. On the basis of nonmetrical cranial variation, it is clear that the samples from Uttar Pradesh, Andhra Pradesh and Bihar are closer to each other but distant from Madhya Pradesh, Karnataka and Maharashtra. On the other hand, Karnataka and Maharashtra samples are quite close to each other and both, in turn, are comparatively closer to Madhya Pradesh than to Uttar Pradesh, Andhra Pradesh and Bihar. Madhya Pradesh sample emerges as the most divergent group among the six population samples studied. This, in general, is in conformity with the picture that emerges from various analysis of morphometric and other biological data on various populations of India.     

Body measurements and somatotypes of young adult Jat-Sikh men of Punjab, India

During 1972 body measurements including height, weight, limb circumferences, skeletal diameters and skinfolds were taken on 100 unrelated and healthy Jat-Sikh men of 17 to 25 years of age, belonging to different areas of Punjab, India. The average height and weight of Jat-Sikhs is 170.4 cm and 54.5 kg, respectively. The mean Heath-Carter somatotype is 3.22-3.40-4.11. The majority of somatotypes is concentrated in endo-ectomorph and meso-ectomorph sectors of the somatochart. Comparisons with recently described data on females of the same area and population show significant sex differences in various body measurements except for the bicristal diameter. The females possess significantly more subcutaneous fat. For the rest of the measurements, the males have higher values. The Jat-Sikh males are comparable in height and weight to the contemporary pooled Punjabi, but are distinctly taller than the neighbouring populations of Himachal Pradesh and pooled all India samples. However, they are comparatively smaller and lighter as compared to European and American populations.     

Pre-Caucasoid and Caucasoid genetic features of the Indian population,revealed by mtDNA polymorphisms.

About 70 individuals from Punjab were examined for some mtDNA polymorphisms, namely, the RFLPs of the six classical enzymes (HpaI, BamHI, HaeII, MspI, AvaII, and Hin-cII) and for the sites AluI(7,025), DdeI(10,394), and AluI(10,397). The AluI(7,025) polymorphic site was also investigated in 96 Indians from Uttar Pradesh and Andhra Pradesh and in 163 Mediterranean Caucasoids. Moreover, 30 Indian DdeI(10,394)Alu(10,397) (++) mtDNAs were typed by the "high-resolution restriction analysis" with 14 endonucleases to estimate their divergence time. The results obtained are the following: (1) The RFLPs analysis has displayed some Caucasoid types as in Indians of Uttar Pradesh; (2) the AluI(7,025) (-) allele, which defines the most frequent Caucasoid-specific lineage (haplogroup H), ranges from 18% to 45% in the Mediterranean Caucasoids, whereas it has shown low frequencies in Punjab (6.0%) and in Uttar Pradesh (1.8%) and was not found in Andhra Pradesh; (3) the DdeI(lO,394)AluI(10,397) (+ +) haplotype, which although previously was considered an East Asian marker (haplogroup M) and was found very frequently in India, is also frequent in Punjab (27%); this frequency is, however, much lower than in Uttar Pradesh (49%) and in Andhra Pradesh (74%), and a gradient decreasing from south to north is therefore observed; (4) the divergence time of the Indian DdeI(10,394)AluI(10,397) (++) mtDNAs has been estimated to be 30,250-60,500 years, a value that is compatible with that of the homologous East Asian lineage. These results strongly support the hypothesis that the DdeI(10,394)AluI(10,397) (++) haplotype predated the Indo-European invasion and probably the split between proto-Indians and proto-Orientals. Its frequency cline well reflects the major influence of Indo-Europeans in the north and in the center of India.     

Red cell enzyme polymorphism in two populations of coastal Andhra Pradesh, South India

Four red cell enzyme polymorphisms: Esterase-D (ESD), Glyoxalase-I (GL01), Superoxide Dismutase (SOD) and Phosphoglucomutase (PGM1 and 2), have been studied in two endogamous populations (paidi and Valmiki) of North Coastal Andhra Pradesh, South India. Inter group comparison by chisquare analysis revealed no significant differences among them. An attempt was made to compare with those available for other populations from Andhra Pradesh.     

Plasma protein polymorphisms in Paidies and Valmikies of coastal Andhra Pradesh, south India

Five plasma protein polymorphisms--Haptoglobin (HP), Group Specific Component (GC), Transferrin (TF), Albumin (ALB) and Caeruloplasmin (CP)--have been determined in two endogamous populations (Paidies and Valmikies) of North Coastal Andhra Pradesh, South India. The results were compared between the two populations. They revealed significant differences for the TF system only. The results were also compared with those available from other Andhra Pradesh populations.     

Red cell glucose-6-phosphate dehydrogenase deficiency among Andhras

Sixty-eight Andhra males and 45 Andhra females from Visakhapatnam town of Andhra Pradesh, India have been investigated for G-6-PD deficiency. The GdB- gene has a frequency of 4.41% among males. No G-6-PH deficient females were detected. The present data have been compared with the available tribal and non-tribal data from Andhra Pradesh. It is observed that the present sample, though non-tribal in nature, presents a relatively considerable frequency of the GdB- gene.     

Database management system for the control of malaria in Arunachal Pradesh, India

The Arunachal Pradesh state in India is epidemic for malaria, caused by P.vivax and P.falciparum. Despite the implementation of several control strategies, the outbreak of malaria in the state is mainly due to lack of proper information regarding the disease. Hence, we completed a database to help implement appropriate control strategy for the public health officials in Arunachal Pradesh. AVAILABILITY: www.envisiict.org.     

Presence of Wolbachia endosymbionts in microfilariae of Wuchereria bancrofti (Spirurida: Onchocercidae) from different geographical regions in India

In view of the recent discovery of rickettsial endosymbionts, Wolbachia in lymphatic filarial parasites, Wuchereria bancrofti and Brugia malayi and subsequently of their vital role in the survival and development of the latter, antibiotics such as tetracycline are being suggested for the treatment of lymphatic filariasis, by way of eliminating the endosymbiont. But, it is essential to assess their presence in parasites from areas endemic for lymphatic filariasis before such a new control tool is employed. In the present communication, we report the detection of Wolbachia endosymbionts in microfilariae of W. bancrofti parasites collected from geographically distant locations of India, such as Pondicherry (Union Territory), Calicut (Kerala), Jagadalpur (Madhya Pradesh), Thirukoilur (TamilNadu), Chinnanergunam (TamilNadu), Rajahmundry (Andhra Pradesh), and Varanasi (Uttar Pradesh), using Wolbachia specific 16S rDNA polymerase chain reaction.     

An assessment of social vulnerability to climate change among the districts of Arunachal Pradesh,India

The present study highlighted the state of climate change induced social vulnerability of the districts of Arunachal Pradesh. For the purpose of assessment of one of the most fragile ecosystems of the eastern Himalaya, the ‘Integrated Vulnerability Assessment Approach’ and IPCC’s definition of vulnerability were utilized. The assessment was based on various secondary data, like socio-economic and biophysical indicators, collected from several authenticated sources; and the respective weightage of these indicators was assigned by using ‘Principal Component Analysis’. Vulnerability was calculated as the net effect of exposure and sensitivity on the adaptive capacity. Anjaw district of eastern Arunachal Pradesh was found to be the most vulnerable district, while Tawang district of western Arunachal Pradesh happened to be the least vulnerable. This net effect was found negative in 7 out of 12 districts viz. Anjaw, Upper Siang, West Siang, Lower Dibang Valley, East Siang, East Kameng and Kurung Kurmey. This net negative effect could be construed as an alarming situation.     

Book Review

To study the variability and to identify the species of Begomovirus associated with yellow mosaic disease of blackgram in Andhra Pradesh, India, infected blackgram samples were collected from six districts belonging to three regions of Andhra Pradesh. The total DNA was isolated by modified CTAB method and amplified with coat protein gene-specific primers (RHA-F and AC abut) resulting in 900?bp gene product. The PCR products were cloned, sequenced and deposited in GenBank. The sequence analysis of six clones showed that the size of amplified CP gene of YMV was 920?bp. Based on nucleotide sequence identity of six isolates representing three regions of Andhra Pradesh, the isolates from Rayalaseema and Telangana region are the same variant of YMV (>99.5% identity) and isolate from coastal Andhra is another variant of YMV (>95.4%) when compared with other region isolates. Comparison of CP gene sequence of YMV-TPT isolate with 27 other isolates in database revealed more than 93.2 and 86.2% identity with MYMIV isolates and less than 80 and 64% identity with MYMY isolates that originate from Indian sub-continent and South-East Asia at nucleotide and amino acid level, respectively. Phylogenetic tree based on CP gene sequences of six isolates with other isolates from GenBank formed unique cluster with MYMIV. Hence the YMV infecting blackgram in Andhra Pradesh is caused by MYMIV rather than MYMY as reported in Tamil Nadu which is adjoining state in southern India.     

Biodegradation of polycyclic aromatic hydrocarbons in soil around Mathura oil refinery,India

Six polycyclic aromatic hydrocarbons [naphthalene, anthracene, phenanthrene, pyrene, chrysene and benzo(a)-pyrene] were detected in soil receiving effluents from an oil refinery. Biodegradation studies revealed a time-dependent disappearance of these polycyclic aromatic hydrocarbons when they were added to soil samples: naphthalene disappeared completely in 60 days, whereas phenanthrene, anthracene, pyrene, chrysene and benzo(a)pyrene decreased by 87%, 34%, 21%, 5% and 40%, respectively, in 120 days.B.T. Ashok and J. Musarrat were and S. Saxena is with the Interdisciplinary Biotechnology Unit, A.M.U., Aligarh-202002, Uttar Pradesh, India. K.P. Singh is with the Environmental Chemistry Section, Industrial Toxicology Research Centre, M.G. Road, Lucknow-226001, Uttar Pradesh, India. B.T. Ashok is now with the Department of Biochemistry, J.N. Medical College, A.M.U., Aligarh-202002, Uttar Pradesh, India. J. Musarrat is now with the Department of Radiology and Blochemistry Program. The Ohio State University, Columbus, OH 43210, USA.     

A genetic study of the Konda Kapu tribe of coastal Andhra Pradesh, South India

Phenotype and gene frequencies of two blood group and four red cell enzyme systems were examined in a Konda Kapu tribal sample of Coastal Andhra Pradesh, South India. The gene frequencies for these systems in Konda Kapus indicate the middle range values for Andhra Pradesh tribal populations, excepting the ADA and Rh(D) systems, where extreme range values are found. Further, gene flow is indicated between the Konda Kapus under study and Plain Kapus, a neighbouring caste population by calculation of Fi estimates.     

Variation in anthropometric characters and their relationship with climatic factors: a study on the peoples of Arunachal Pradesh, India

In the present study an attempt has been made to examine the anthropometric variation and the relationship of anthropometric variables with climatic factors among the peoples of Arunachal Pradesh, India. The distance analysis does not seem to provide any interesting corroboration between anthropometric data and the intra- and intergroup relationship. A similar trend has also been noticed while analysing the correlation coefficient between two indices (i.e. the cephalic and total facial index). The present study suggests that the climatic factors are playing an important role in regulating the variation of anthropometric characters among the different population groups of Arunachal Pradesh.     

Investigation and mapping of fluoride-endemic areas and associated health risk—A case study of Agra,Uttar Pradesh,India

The present study aimed at identifying the fluoride-endemic areas in five different blocks in Agra district, Uttar Pradesh, India. A total of 365 groundwater samples from 73 villages were analyzed for establishing the concentration range of fluoride in drinking water. The fluoride level in the study area varied from 0.14 to 4.88 mg/L. Out of 73, the fluoride levels in 45 villages did not meet the permissible Word Health Organization standards. The Baroli Ahir block was found the highly fluoride-endemic area followed by Saiyan, Bichpuri, Achnera and Etmadpur. Chronic daily intake of fluoride in adults was 1.25 and 1.5 times higher than those in children and infants, respectively. The probability of dental fluorosis in infants was higher (42%) while adults were more prone to bone and skeletal fluorosis (60%). The hazard quotient analysis revealed that children were found to be at maximum risk followed by infants and adults. Sensitivity analysis revealed that the fluoride concentration is the major influencing parameter responsible for different types of fluorosis in various age groups.     

Morphological variation in the sugarcane smut Ustilago scitaminea Syd

Summary A study of the morphological variability among 25 different collections of sugarcane smut fungusUstilago scitaminea from the States of Delhi, Uttar Pradesh, Bihar, Madhya Pradesh, Maharastra, Andhra Pradesh and Madras revealed that considerable variation exists in spore size, colour, spore-wall markings of the isolates. On the basis of statistically significant differences in the spore size, the 25 isolates can be classified into 4 distinct groups. Three types of spore-wall markings viz., verrucose, punctate and echinulate are met within the isolates studied. There is no appreciable difference in the spore wall thickness of the different isolates. Spore colour of the different isolates varied from yellow to brown with several shades in between.Part of a thesis submitted by the senior author in partial fulfilment of the Degree of Doctor of Philosophy, P. G. School, Indian Agricultural Research Institute, New Delhi.     

Histopathology of Mycotoxicosis produced in Swiss albino mice by metabolites of some fungal isolates.

Of 199 fungal cultures isolated from some of the common cereals collected from different parts of Uttar Pradesh and Madhya Pradesh, 70 produced toxic metabolites. Of the 70 fungi isolated, 59 produced toxins which caused visible lesions in livers, kidneys, and spleens but did not cause mortality. Toxicity, graded in terms of the mortality rate and the extent of lesions in livers, kidneys, and spleens, was found to be highest in various species of Aspergillus (40%), followed by Chaetomium spp. (31%).     

Pholidota pygmaea (Orchidaceae) — A new species from Arunachal Pradesh, India

Chowdhery, H.J. & Pal, G.D. 1995. Pholidota pygmaea (Orchidaceae) — A new species from Arunachal Pradesh, India. — Nord. J. Bot. 15: 411–413. Copenhagen. ISSN 0107–055X.
A new species of Pholidota, P. pygmaea from Arunachal Pradesh, India is described.     

Age at return marriage and timing of first birth in India's Uttar Pradesh and Kerala states.

The study investigates the relationship between age at marriage and the length of first birth interval in two states of India: Uttar Pradesh and Kerala. Life tables of first-birth intervals and median first-birth intervals are computed for several subgroups of the study population. Multivariate hazards modelling technique is used to study the net effect of age at marriage, controlling for a multiple of socioeconomic factors. The result shows that the average first-birth interval varies by age at marriage and is much longer in Uttar Pradesh than in Kerala.     

Characterization of <Emphasis Type="Italic">Bradyrhizobium</Emphasis> Strains Isolated from Different Varieties of Soybean with 16SrDNA RFLP from Agricultural Land of Madhya Pradesh,India

Madhya Pradesh is the major soybean contributor in India. The taxonomy of nitrogen fixing bacteria forming symbiotic associations with leguminous plants has been deeply changed in recent years. The use of very sensitive and accurate molecular methods has enabled the detection of large rhizobial diversity. Molecular biotyping and characterization the Bradyrhizobium, isolates from eleven varieties of soybean from agricultural field of Sehore district of Madhya Pradesh is done using 16S rDNA typing. Bradyrhizobia were identified genetically by determining the %Guanine plus Cytosine content of the whole genome, followed by 16S rDNA-RFLP analysis % Guanine plus Cytosine content of all the Bradyrhizobium isolates reflects similarity at generic level among all Bradyrhizobial isolates. Restriction Fragment Length Polymorphism (RFLP) further showed a considerable level of genetic diversity among the Bradyrhizobial isolates. PCR-RFLP of 16S rDNA supported existence of two divergent groups among indigenous Bradyrhizobial isolates, at similarity level of 66, and 75 and 74% of similarity within the group. The technique used was helpful in characterizing Bradyrhizobium isolates to be used as inoculants for improving productivity of agricultural land of Madhya Pradesh (India).