共查询到20条相似文献,搜索用时 31 毫秒
1.
A Gram-stain-negative, non-motile, strictly aerobic, yellow-pigmented bacterium, designated strain 5G38 T, was isolated from a field cultivated with Chinese cabbage in Korea. The strain grew at 5–40°C and at pH 6.0–8.0. 16S rRNA gene sequence analysis revealed that strain 5G38 T represented a distinct lineage within the family Sphingobacteriaceae and showed the highest 16S rRNA gene sequence similarity of 95.2% with Pedobacter koreensis WPCB189 T, followed by Pedobacter agri PB92 T (94.6%), Pedobacter suwonensis 15–52 T (94.4%), Pedobacter rhizosphaerae 01–96 T (94.4%), Pedobacter sandarakinus DS-27 T (94.4%), and Nubsella zeaxanthinifaciens TDMA-5 T (94.3%). Strain 5G38 T formed monophyletic clade with Nubsella zeaxanthinifaciens in the cluster comprised of species of the genus Pedobacter. Chemotaxonomic characteristics of the novel strains, including DNA G+C content of genomic DNA (37.0 mol%), the predominant respiratory quinine (MK-7), and the major fatty acids which were iso-C 15:0, summed feature 3 (comprising C 16:1 ω7 c and/or iso-C 15:0 2-OH) and iso-C 17:0 3-OH, are similar to those of the genus Pedobacter. However, the novel strains can be distinguished from the other species of Pedobacter by physiological properties. The name Pedobacter namyangjuensis sp. nov. is therefore proposed for strain 5G38 T (KACC 13938 T =NBRC 107692 T) as the type strain. Furthermore, the reclassification of Nubsella zeaxanthinifaciens as Pedobacter zeaxanthinifaciens comb. nov. is proposed. 相似文献
2.
A Gram-stain negative, strictly aerobic, motile by gliding, rod-shaped and yellow pigmented strain THG-G12 T was isolated from soil of a bamboo field in Seoul, Republic of Korea. Strain THG-G12 T was observed to grow well at 20–28 °C and pH 7.0–7.5 in the absence of NaCl on nutrient agar. Based on 16S rRNA gene sequence comparisons, strain THG-G12 T was found to be most closely related to Pedobacter ginsengisoli Gsoil 104 T (97.5 % sequence similarity), Pedobacter steynii WB2.3-45 T (97.4 %), Pedobacter metabolipauper WB2.3-71 T (97.2 %), Pedobacter nyackensis NWG-II14 T (97.2 %), Pedobacter caeni LMG 22862 T (97.1 %) and Pedobacter duraquae WB2.1-25 T (97.0 %), but DNA relatedness between strain THG-G12 T and its phylogenetically closest neighbours was below 9.5 %. The G+C content of the genomic DNA was determined to be 39.9 mol%. The only isoprenoid quinone detected in strain THG-G12 T was menaquinone-7 (MK-7). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids were found to be phosphatidylethanolamine, unidentified phosphoglycolipids, unidentified aminophosphoglycolipids, unidentified aminolipids and unidentified lipids. Strain THG-G12 T showed the presence of two ceramide phosphorylethanolamines (CerPE-2′ and CerPE-2″), dihydrosphingosines and an unidentified ceramide as the major ceramide. The major fatty acids were identified as summed feature 3 (as defined by the MIDI system; C 16:1 ω7 c and/or C 16:1 ω6 c) and iso-C 15:0. These data support the affiliation of strain THG-G12 T to the genus Pedobacter. The results of physiological and biochemical tests enabled strain THG-G12 T to be differentiated genotypically and phenotypically from the recognized species of the genus Pedobacter. Therefore, the novel isolate represents a novel species, for which the name Pedobacter seoulensis sp. nov. is proposed, with THG-G12 T as the type strain (=KACC 17529 T =JCM 19363 T). 相似文献
3.
A novel strain, DCY108 T was isolated from soil of a Panax ginseng field, Yeoncheon province (38°04′N 126°57′E), Republic of Korea. Strain DCY108 T is Gram-negative, non-motile, non-flagellate, rod-shaped, and aerobic. The bacterium grows optimally at 25–30 °C, pH 6.5–7.0 and 1 % NaCl. Phylogenetically, strain DCY108 T is closely related to Pedobacter jejuensis JCM 18824 T, Pedobacter aquatilis JCM 13454 T, Pedobacter kyungheensis LMG 26577 T and the type strain of the genus Pedobacter heparinus DSM 2366 T. The DNA–DNA relatedness values between strain DCY108 T and its close phylogenetic neighbors were below 30.0 %. The DNA G+C content of strain DCY108 T was determined to be 45.1 mol%. The predominant quinone was menaquinone 7 (MK-7). The major polar lipids were identified as phosphatidylethanolamine and three unidentified aminolipids AL1, AL13 and AL17. Iso-C 15:00, iso-C 17:03OH and summed feature 3 (C 16:1 ω7 c/C 16:1 ω6 c) were identified as the major fatty acids present in strain DCY108 T. The results of physiological and biochemical tests allowed strain DCY108 T to be differentiated phenotypically from other recognized species belonging to the genus Pedobacter. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Pedobacter panacis sp. nov is proposed with the type strain designated as DCY108 T (=CCTCCAB 2015196 T = KCTC 42748 T). 相似文献
4.
Strain HME6451 T was isolated from Lake Soyang in Korea. Phylogenetic tree based on 16S rRNA gene sequence showed that strain HME6451 T formed a lineage within the genus Pedobacter. The strain HME6451 T was closely related to Pedobacter daechungensis (95.4% sequence similarity), Pedobacter lentus (94.4%), and Pedobacter glucosidilyticus (93.8%). And strain HME6451 T was a Gram-staining-negative, short rod-shaped, strictly aerobic bacterium. The major fatty acids were iso-C 15:0 (41.2%), summed feature 3 (comprising C 16:1 ω7 c and/or C 16:1 ω6 c; 23.1%), and iso-C 17:0-3OH (10.1%). The polar lipids of HME6451 T were consisted of one phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid and four unidentified polar lipids. The DNA G+C content was 36.0 mol%. On the basis of the evidence presented in this study, strain HME6451 T represent a novel species of the genus Pedobacter, for which the name Pedobacter soyangensis sp. nov., is proposed the type strain HME6451 T (=KCTC 23467 T =CECT 7865 T). 相似文献
5.
A Gram-negative, strictly aerobic, non-motile, rod-shaped and psychrophilic bacterial strain, PAMC 27137 T, was isolated from the marine sediment of the Ross Sea, Antarctica. Strain PAMC 27137 T was observed to grow at 4–10 °C, at pH 6.5–7.5 and in the presence of 2.5–4.0 % (w/v) sea salts. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PAMC 27137 T belongs to the genus Lacinutrix showing the high similarities with Lacinutrix mariniflava JCM 13824 T (97.6 %) and Lacinutrix algicola JCM 13825 T (97.1 %). Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27137 T is clearly distinguished from the most closely related Lacinutrix species. The major fatty acids (>5 %) were identified as iso-C 15:1 G (19.9 %), iso-C 15:0 (19.3 %), iso-C 17:0 3-OH (11.3 %), summed feature 9 (C 16:0 10-methyl and/or iso-C 17:1 ω9 c as defined by MIDI, 9.1 %), iso-C 15:0 3-OH (7.5 %), and anteiso-C 15:1 A (5.8 %). The polar lipids were found to consist of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and five unidentified phospholipids. The major respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 32.1 mol%. Based on the data from this polyphasic taxonomic study, strain PAMC 27137 T is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix jangbogonensis sp. nov. is proposed. The type strain is PAMC 27137 T (=KCTC 32573 T=JCM 19883 T). 相似文献
7.
A Gram-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated M-M16 T, was isolated from seashore sand around a seaweed farm on the South Sea, South Korea, and its taxonomic position was investigated by using a polyphasic study. Strain M-M16 T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Strain M-M16 T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Gaetbulibacter lutimaris (96.5 %) and Flaviramulus basaltis (95.8 %). Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M16 T clustered with the type strains of Gaetbulibacter species and F. basaltis. Strain M-M16 T contained MK-6 as the predominant menaquinone and iso-C 15:1 G, iso-C 15:0 and iso-C 17:0 3-OH as the major fatty acids. The major polar lipids detected in strain M-M16 T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain M-M16 T was 37.4 mol%. The phylogenetic and chemotaxonomic data and other phenotypic properties revealed that strain M-M16 T represents a novel genus and species within the family Flavobacteriaceae, for which the name Sabulilitoribacter multivorans gen. nov., sp. nov. is proposed. The type strain of S. multivorans is M-M16 T (= KCTC 32326 T = CCUG 63831 T). 相似文献
8.
A strain designated as S85 T was isolated from a seaweed collected from coastal area of Chuuk State in Micronesia. The strain was gram-negative, rod-shaped, and non-motile and formed yellow colonies on the SWY agar (0.2 % yeast extract and 1.5 % agar in seawater) and Marine agar 2216. The strain grew at pH 5–9 (optimum, pH 8), at 15–40 °C (optimum, 25–28 °C), and with 1–9 % (w/v) NaCl (optimum, 3 %). The phylogenetic analysis based on 16S rRNA gene sequence showed that strain S85 T was related to Lutibacter litoralis CL-TF09 T and Maritimimonas rapanae A31 T with 91.4 % and with 90.5 % similarity, respectively. The dominant fatty acids were iso-C 15:0, iso-C 15:0 3-OH and iso-C 17:0 3-OH, C 16:0 3-OH and summed feature 3 (C 16:1 ω7c and/or iso-C 15:0 2-OH). The major isoprenoid quinone was MK-6. The DNA G+C content of the type strain was 34.6 mol %. The major polar lipids were phosphatidylethanolamine, an unknown glycolipid and two unknown polar lipids. Based on this polyphasic taxonomic data, strain S85 T stands for a novel species of a new genus, and we propose the name Ochrovirga pacifica gen. nov., sp. nov. The type strain of O. pacifica is S85 T (=KCCM 90106 =JCM 18327 T). 相似文献
9.
A Gram-negative, non-flagellated, non-gliding and rod-shaped, coccoid or filamentous bacterial strain, designated YCS-16 T, was isolated from coastal seawater from a seaweed farm on the South Sea, South Korea, and its taxonomic position was investigated by using a polyphasic study. Strain YCS-16 T was observed to grow optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. Strain YCS-16 T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Bizionia echini (96.1 %), Formosa spongicola (95.8 %), Bizionia algoritergicola (95.5 %) and Psychroserpens mesophilus (95.4 %). Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain YCS-16 T joined the cluster comprising the type strains of Psychroserpens species. Strain YCS-16 T was found to contain MK-6 as the predominant menaquinone and iso-C 17:0 3-OH, iso-C 15:1 G and iso-C 15:0 as the major fatty acids. The major polar lipids detected in strain YCS-16 T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain YCS-16 T was determined to be 35.7 mol%. The phylogenetic analysis, chemotaxonomic data and other phenotypic properties revealed that strain YCS-16 T constitutes a new genus and species within the family Flavobacteriaceae, for which the name Geojedonia litorea gen. nov., sp. nov. is proposed. The type strain of Geojedonia litorea is YCS-16 T (=KCTC 32260 T = CCUG 63682 T). 相似文献
10.
A Gram-staining-negative, non-spore-forming, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated M-M3 T, was isolated from marine sand on the southern coast of South Korea and subjected to a polyphasic study. Strain M-M3 T was observed to grow optimally at 25–30 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M3 T fell within the clade comprising Nonlabens species, joining the type strain of Nonlabens ulvanivorans, with which it exhibited the highest 16S rRNA gene sequence similarity value of 99.4 %. Sequence similarity to the type strains of the other Nonlabens species was 93.0–95.2 % and <92.2 % to those of other species used in the phylogenetic analysis. Strain M-M3 T was found to contain MK-6 as the predominant menaquinone and iso-C 15:0 and iso-C 17:0 3-OH as the major fatty acids. The major polar lipids were determined to be phosphatidylethanolamine, five unidentified lipids and one unidentified aminolipid. The DNA G+C content of strain M-M3 T was determined to be 38.2 mol% and its DNA–DNA relatedness values with N. ulvanivorans DSM 22727 T was 42 ± 6.2 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain M-M3 T is separate from other Nonlabens species. On the basis of the data presented, strain M-M3 T is considered to represent a novel species of the genus Nonlabens, for which the name Nonlabens arenilitoris sp. nov. is proposed. The type strain is M-M3 T (=KCTC 32109 T = CCUG 62919 T). 相似文献
12.
A non-motile, pink-pigmented bacterial strain designated IMCC25679 T, was isolated from freshwater Lake Chungju of Korea. Phylogenetic trees based on 16S rRNA gene sequences showed that the strain IMCC25679 T formed a lineage within the genus Pedobacter. The strain IMCC25679 T was closely related to Pedobacter daechungensis Dae 13 T (96.4% sequence similarity), Pedobacter rivuli HME8457 T (95.3%) and Pedobacter lentus DS-40 T (94.3%). The major fatty acids of IMCC- 25679 T were iso-C 15:0, iso-C 16:0 and summed feature 3 (comprising C 16:1ω6 c and/or C 16:1ω7 c). The major respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine (PE), an unidentified sphingolipid (SL), an unidentified aminolipid (AL) and three unidentified polar lipids (PL). The DNA G + C content of IMCC25679 T was 32.2 mol%. Based on the evidence presented in this study, the strain IMCC25679 T represents a novel species within the genus Pedobacter, with the proposed name Pedobacter aquicola, sp. nov. The type strain is IMCC25679 T (= KACC 19486 T = NBRC113131 T). 相似文献
13.
A novel halophilic actinomycete strain, H23 T, was isolated from a Saharan soil sample collected in Djamâa (Oued Righ region), El-Oued province, South Algeria. Strain H23 T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23 T had 16S rRNA gene sequence similarities ranging from 97.8 % ( Actinopolyspora xinjiangensis TRM 40136 T) to 94.8 % ( Actinopolyspora mortivallis DSM 44261 T). The strain grew optimally at pH 6.0–7.0, 28–32 °C and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23 T had MK-10 (H 4) and MK-9 (H 4) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C 17:0 (37.4 %), iso-C 17:0 (14.8 %), iso-C 15:0 (14.2 %), and iso-C 16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23 T (=DSM 45501 T = CCUG 63368 T = MTCC 11562 T). 相似文献
14.
A novel Gram-negative, facultatively anaerobic and motile bacterial strain, designated KMM 6351 T, was isolated from the sea urchin Strongylocentrotus intermedius and examined using a polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain formed a distinct phyletic line in the class Gammaproteobacteria and was most closely related to the genera Aliivibrio, Photobacterium and Vibrio. Strain KMM 6351 T grows at 4–40 °C and with 0.5–12 % NaCl and decomposes aesculin, agar, gelatin, starch, chitin and DNA. The DNA G+C content of the strain was determined to be 46.1 mol%. The prevalent fatty acids were found to be C 16:0, C 18:1 ω7c, C 12:0 3-OH and summed feature 3 (comprising C 16:1 ω7c and/or iso-C 15:0 2-OH fatty acids). The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The predominant ubiquinone was found to be Q-8. The results of the phenotypic, chemotaxonomic and genotypic analyses clearly indicated that the novel strain should be assigned to a new genus and species within the class γ- Proteobacteria for which the name Echinimonas agarilytica gen. nov., sp. nov. is proposed. The type strain is KMM 6351 T (=KCTC 22996 T = LMG 25420 T). 相似文献
15.
A novel bacterial strain, designated WS-MY5 T, capable of degrading a variety of polysaccharides was isolated from a brown algae ( Undaria pinnatifida) reservoir at Wando in the South Sea, South Korea. Strain WS-MY5 T was found to grow optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WS-MY5 T falls within the clade comprising Winogradskyella species, clustering with the type strains of Winogradskyella pacifica, Winogradskyella arenosi, Winogradskyella rapida and Winogradskyella thalassocola, with which it exhibited 16S rRNA gene sequence similarity values of 97.3–98.8 %. It exhibited sequence similarity values of 93.0–96.2 % to the type strains of the other recognized Winogradskyella species. Strain WS-MY5 T was found to contain MK-6 as the predominant menaquinone and anteiso-C 15:0, iso-C 15:0, iso-C 15:0 3-OH, iso-C 17:0 3-OH and iso-C 15:1 G as the major fatty acids. The major polar lipids of strain WS-MY5 T were identified as phosphatidylethanolamine, two unidentified lipids and two unidentified aminolipids. The DNA G+C content of strain WS-MY5 T was determined to be 33.2 mol% and its DNA–DNA relatedness values with the type strains of W. pacifica, W. arenosi, W. rapida and W. thalassocola were in the range 16–28 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, enabled strain WS-MY5 T to be differentiated from the recognized Winogradskyella species. On the basis of the data presented here, strain WS-MY5 T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella undariae sp. nov. is proposed. The type strain is WS-MY5 T (=KCTC 32261 T=CCUG 63832 T). 相似文献
16.
A novel bacterial strain designated CJ43T was isolated from fresh water located in Gangwon-do, South Korea, displaying multi-drug resistance. The isolate was Gram-stain-negative, aerobic, orange-pigmented, and rod-shaped. Strain CJ43T grew optimally at 30 °C and pH 7 on R2A agar in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain CJ43T belonged to the genus Pedobacter in the family Sphingobacteriaceae and was most closely related to Pedobacter puniceum HX-22-1 T and P. glucosidilyticus 1-2 T (98.3 and 98.1% sequence similarity). The genome size of strain CJ43T was 3.9 Mb in a single contig with DNA G?+?C content of 34.9%. The genome included 3144 predicted protein-coding genes, as well as 55 tRNA, 9 rRNA and 3 ncRNA genes. The genome also contained 128 putative antibiotic resistance genes, reflecting its phenotypes. The average nucleotide identity values between strain CJ43T and two closely related strains P. puniceum HX-22-1 T and P. glucosidilyticus 1-2 T were 91.0 and 88.7%, respectively. In silico digital DNA-DNA hybridization results between strain CJ43T and the related strains were 42.8 and 38.6%, respectively. The major fatty acids of strain CJ43T were iso-C15:0, iso-C17:0 3-OH, and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c). Strain CJ43T contained phosphatidylethanolamine as the major polar lipid and menaquinone-7 as the sole respiratory quinone. Based on the polyphasic taxonomy data, strain CJ43T represents a novel species of the genus Pedobacter, for which the name Pedobacter aquae sp. nov. is proposed with the type strain CJ43T (=?KACC 21350 T?=?JCM 33709 T).
相似文献
17.
A Gram-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, GGW-M5 T, was isolated from seawater on the southern coast in Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain GGW-M5 T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain GGW-M5 T belonged to the genus Glaciecola, joining the cluster comprising the type strains of G. agarilytica, G. arctica, G. chathamensis, G. mesophila, G. polaris and G. psychrophila, with which it exhibited 16S rRNA gene sequence similarity values of 95.9–96.7 %. Strain GGW-M5 T exhibited sequence similarity values of 93.2–94.8 % to the type strains of the other Glaciecola species. Strain GGW-M5 T contained Q-8 as the predominant ubiquinone and C 16:1 ω7 c and/or iso-C 15:0 2-OH, C 16:0 and C 14:0 2-OH as the major fatty acids. Major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G+C content was 42.4 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain GGW-M5 T could be distinguished from other Glaciecola species. On the basis of the data presented, strain GGW-M5 T is considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola aquimarina sp. nov. is proposed. The type strain is GGW-M5 T (=KCTC 32108 T = CCUG 62918 T). 相似文献
18.
A Gram-negative, non-spore-forming, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated P-R2A1-2 T, was isolated from sea squirt ( Halocynthia roretzi) collected from the South Sea, Korea. It grew optimally at 25–28 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that the strain fell within the clade comprising Tenacibaculum species. Strain P-R2A1-2 T exhibited the highest 16S rRNA gene sequence similarity values of 97.6, 97.2 and 97.0 % to Tenacibaculum aestuarii SMK-4 T, T. lutimaris TF-26 T and T. aiptasiae a4 T, respectively, and of 94.5–96.8 % to the type strains of the other Tenacibaculum species. Strain P-R2A1-2 T contained MK-6 as the predominant menaquinone and C 16:1 ω7 c and/or iso-C 15:0 2-OH, iso-C 15:0 3-OH and iso-C 15:0 as the major fatty acids. The DNA G + C content of strain P-R2A1-2 T was 30.7 mol % and its DNA–DNA relatedness values with the type strains of T. aestuarii, T. lutimaris and T. aiptasiae were 17 ± 4.2, 21 ± 6.1 and 16 ± 5.2 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separate from other Tenacibaculum species. On the basis of the data presented, strain P-R2A1-2 T is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum halocynthiae sp. nov. is proposed. The type strain is P-R2A1-2 T (=KCTC 32262 T = CCUG 63681 T). 相似文献
19.
A Gram-stain negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated GYSW-15 T, was isolated from a seawater of Geoje island on the South Sea, South Korea, and subjected to a polyphasic taxonomic approach. Strain GYSW-15 T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GYSW-15 T belongs to the genus Polaribacter, clustering coherently with the type strain of Polaribacter porphyrae showing sequence similarity value of 97.0 %. Strain GYSW-15 T exhibited 16S rRNA gene sequence similarity values of 94.3–96.4 % to the type strains of the other Polaribacter species. Strain GYSW-15 T was found to contain MK-6 as the predominant menaquinone and iso-C 15:0, iso-C 15:1 G, iso-C 16:0 3-OH and summed feature 3 (C 16:1 ω7 c and/or C 16:1 ω6 c) as the major fatty acids. The polar lipid profile of strain GYSW-15 T containing phosphatidylethanolamine and an unidentified lipid as major components was similar to that of the type strain of P. porphyrae. The DNA G + C content of strain GYSW-15 T was determined to be 31.2 mol% and its mean DNA–DNA relatedness with P. porphyrae LMG 26671 T was 17 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GYSW-15 T is separated from recognized Polaribacter species. On the basis of the data presented, strain GYSW-15 T is considered to represent a novel species of the genus Polaribacter, for which the name Polaribacter marinivivus sp. nov. is proposed. The type strain is GYSW-15 T ( = KCTC 42156 T = CECT 8655 T) 相似文献
20.
A Gram-negative, aerobic, non-flagellated and rod-shaped bacterial strain able to move by gliding, designated TYO-10 T, was isolated from an oyster collected at Tongyoung on the South Sea, South Korea. Strain TYO-10 T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain TYO-10 T joined the cluster comprising the type strains of Mariniflexile species. Strain TYO-10 T exhibited 16S rRNA gene sequence similarity values of 95.4–95.7 % to the type strains of Mariniflexile species and of less than 95.4 % to the type strains of other recognized species. Strain TYO-10 T was found to contain MK-6 as the predominant menaquinone and iso-C 15:0, iso-C 15:1 G, anteiso-C 15:0, iso-C 17:0 3-OH and iso-C 15:0 3-OH as the major fatty acids. The major polar lipids of strain TYO-10 T were identified as phosphatidylethanolamine and an unidentified lipid, which is similar to those of the type strains of Mariniflexile species, but different from those of other phylogenetically related species. The DNA G+C content of strain TYO-10 T was determined to be 35.9 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain TYO-10 T is separated from other Mariniflexile species. On the basis of the data presented, strain TYO-10 T is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile ostreae sp. nov. is proposed. The type strain is TYO-10 T (= KCTC 42113 T = CECT 8622 T). 相似文献
|