共查询到20条相似文献,搜索用时 8 毫秒
1.
Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase 总被引:1,自引:0,他引:1
E A Stura D L Johnson J Inglese J M Smith S J Benkovic I A Wilson 《The Journal of biological chemistry》1989,264(16):9703-9706
Crystals of glycinamide ribonucleotide transformylase have been grown from 0.4 to 1 M ammonium sulfate, 0.6 to 1 M sodium-potassium phosphate, or 0.65 to 1 M citrate in the pH range 4.5-7.0. The single crystals display variable morphology with varying pH. The crystals belong to the orthorhombic space group C222 with cell dimensions a = 141.4 A, b = 98.2 A, c = 103.5 A. Co-crystals have also been obtained in the presence of the inhibitor 5,8-dideazafolate (KI = 18 microM) under similar crystallization conditions. Crystals of a chemically modified enzyme, iodinated at Cys-21, were grown under similar conditions within the pH range 6.5-7.0. These crystals are isomorphous with the unmodified enzyme. Crystals suitable for high resolution (less than 2.5 A) x-ray diffraction studies have been obtained for each of the above. 相似文献
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Lactoglobulin isolated from Italian buffalo milk has been crystallized in two different forms. Crystals of type I have been grown at pH 3.5 and belong to space group P63; the unit cell dimensions are , and accordingly the asymmetric unit contains one dimer of molecular weight 36,000. Type II crystals, grown at pH 8.5, have only one monomer per asymmetric unit; the space group is P3121 (or enantiomorph) and the unit cell edges are: . The crystals of the trigonal form show low radiation damage and are suitable for a structural investigation. 相似文献
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C D Stout 《Journal of molecular biology》1978,126(1):105-108
Two closely related crystal forms of dimeric cytochrome c5 from Azotobacter rinelandii have been grown. The crystals belong to space groups (C2 with ; and C1 (a centered triclinic cell) with , α = 87 ° 20′, β = 96 ° 40′ and γ = 90 ° 0′. In C2 the 24,000 molecular weight dimer lies on a Crystallographic 2-fold axis; in C1 the entire dimer occupies the asymmetric unit. 相似文献
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Glycolate oxidase, an enzyme that plays an important role in photorespiration in plants, has been purificant from spinach and crystallized in two different crystal forms. Form A which was obtained with tertiary butanol as precipitating agent belongs to space group I 422 with unit cell dimensions a = b = 148.1 A and c = 134.9 A. This form diffracts to high resolution and will be used for further crystallographic studies. Form B is also tetragonal, space group P42212, with cell dimensions a = b = 145.4 A and c = 104.2 A. This form was obtained from ammonium sulfate precipitations. Sodium dodecyl sulfate polyacrylamide gel electrophoresis shows that the enzyme is built up from subunits of molecular weight 37,000. The asymmetric units of both crystal forms contain at least two such subunits. 相似文献
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E. C. Sweeney A. G. Tonevitsky D. E. Temiakov I. I. Agapov S. Saward R. A. Palmer 《Proteins》1997,28(4):586-589
The quaternary structure of ricin agglutinin (RCA) has been determined by x-ray crystallography. The refined structure of ricin proved to be a successful search model using the molecular replacement method of phase determination. RCA forms an elongated molecule of dimensions 120 Å × 60 Å × 40 Å with two A chains at the center and a B chain at each end. The A chains are covalently associated via a disulfide bridge between Cys 156 of both chains. Additional contacts at residues 114–115 stabilize the dimer interface. The covalent association of RCA A chains was confirmed by gel filtration under reducing and nonreducing conditions. Proteins 28:586–589, 1997. © 1997 Wiley-Liss, Inc. 相似文献
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Preliminary crystallographic study of the Fab fragments of two monoclonal anti-2-phenyloxazolone antibodies 总被引:2,自引:0,他引:2
R A Mariuzza G Boulot V Guillon R J Poljak C Berek J M Jarvis C Milstein 《The Journal of biological chemistry》1985,260(18):10268-10270
We report on the preparation, crystallization, and preliminary x-ray crystallographic study of the Fab fragments of two monoclonal anti-2-phenyloxazolone antibodies obtained from the secondary response to this hapten. The Fab fragment from one of these (NQ10/12.5) has been crystallized from polyethylene glycol 8000 solutions in a form suitable for high-resolution x-ray crystallographic studies. These crystals are monoclinic, space group C2, with a = 129.2 A, b = 79.4 A, c = 57.7 A, beta = 96.2 degrees, and one Fab/asymmetric unit. Determination of the three-dimensional structure of Fab NQ10/12.5 should help clarify the role of somatic mutation in the maturation of an immune response. This antibody and an anti-lysozyme antibody also under study apparently use the same germ-line encoded VK and a similar VH gene, respectively, as the idiotypic anti-oxazolone antibodies characteristic of the primary response. A comparative study of the two structures should shed light on the role of the pairing of heavy and light chains in the antigen-binding function of antibodies. 相似文献
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P R Kolatkar M A Oliveira M G Rossmann A H Robbins S K Katti H Hoover-Litty C Forte J M Greve A McClelland N H Olson 《Journal of molecular biology》1992,225(4):1127-1130
Crystals of the two amino-terminal domains of intercellular adhesion molecule-1, the receptor for the major group of human rhinovirus serotypes, diffract to 3.0 A resolution. The crystals are trigonal in space group P3(1)21 or P3(2)21 with cell dimensions of a = b = 55.7 A, c = 166.3 A, with probably six molecules per unit cell. 相似文献
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E Lolis R M Sweet L S Cousens P Tekamp-Olson B A Sherry A Cerami 《Journal of molecular biology》1992,225(3):913-915
Macrophage inflammatory protein 2 (MIP-2) has been crystallized by vapor diffusion of an 11 mg/ml protein solution in 100 mM-ammonium acetate against 30 to 40% polyethylene glycol (average molecular mass of 3350 Da). The crystals belong to space group P2(1)2(1)2(1) and have unit cell dimensions of a = 42.7 A, b = 59.3 A, and c = 100.3 A. The molecular mass of the protein and volume of the unit cell suggest that there are four monomers in the asymmetric unit. A data set to 2.3 A has been collected, and the self-rotation function identifies the presence of a non-crystallographic 2-fold axis. 相似文献
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Crystals of a trypsin-modified alkaline phosphatase. Preliminary crystallographic characterization 总被引:1,自引:0,他引:1
S Olafsdottir C Wright H T Wright J F Chlebowski 《The Journal of biological chemistry》1988,263(20):10002-10004
Trypsin-modified alkaline phosphatase from Escherichia coli has been crystallized in a form distinct from the two known crystal forms of the native enzyme. The large well diffracting crystals belong to the orthorhombic space group P2(1)2(1)2(1), possess unit cell dimensions a = 56.0 A, b = 136.0 A, c = 283.9 A with 2 dimers per asymmetric unit, and are suitable for high resolution x-ray crystallographic studies. The observed structural and functional differences between the native and modified molecules are a result of peptide bond cleavage at Arg10-Ala11 with loss of the NH2-terminal decapeptide in both subunits of the dimer. 相似文献
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A well characterized soybean protease inhibitor, the Bowman-Birk inhibitor, has been crystallized at room temperature in the presence of polyethylene glycol 4000 by vapor diffusion against an ammonium sulfate solution containing 2-methyl-2,4-pentanediol. An x-ray diffraction study reveals that the inhibitor crystallizes in a hexagonal unit cell of symmetry P6122 (or P6522) and dimensions a = b = 91.36(2) A and c = 63.92(2) A. Each of the 12 asymmetric units contains 2 molecules of molecular weight 8000. The crystal, which diffracts barely to 3-A spacings, is fairly stable to x-irradiation and has a solvent content of approximately 52% by volume. 相似文献
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E J Gordon S M Bury L Sawyer S Atrian R Gonzalez-Duarte 《Journal of molecular biology》1992,227(1):356-358
The alcohol dehydrogenase (ADHase) enzyme catalyses the oxidation of alcohols to aldehydes or ketones using NAD+ as a cofactor. Functional ADHase from Drosophila lebanonensis is a dimer, with a monomeric molecular weight of 27,000 and with 254 residues in each polypeptide chain. Crystals of the protein have been grown with and without NAD+. Two crystal forms have been observed. Most crystals are plate-like, 0.05 mm in their shortest dimension and up to 0.4 mm in their longest dimension. These crystals are generally too small to diffract efficiently using conventional X-ray sources, so preliminary studies were carried out using the Synchrotron Radiation Source at the SERC Daresbury Laboratory. Twinning was a severe problem with this crystal form. The second form is grown in the absence of NAD+ but with DL-dithiothreitol present. These crystals grow more evenly and diffract to better than 2 A resolution. They are monoclinic, with cell dimensions, a = 81.24(6) A, b = 55.75(4) A, c = 109.60(7) A and beta = 94.26(9) degrees, space group P2(1). There are two dimers in the asymmetric unit, but at low resolution a rotated cell with one dimer per asymmetric unit can be obtained. 相似文献
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Beta-lytic protease from Myxobacter 495 was crystallized by dialysis with 1 m-sodium chloride and 0.1 m-sodium citrate (pH 5.95). The crystals were rhombic prisms with space group P212121 and unit cell parameters a = 54.1, b = 99.6 and . Considerations of the cell volume and molecular weight indicate two molecules of beta-lytic protease in the asymmetric unit. 相似文献
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Crystals of lysozyme from Streptomyces erythraeus are orthorhombic, space group P212121 with unit cell dimensions: and ; and one molecule, of molecular weight about 18,500, per asymmetric unit. 相似文献
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Preliminary crystallographic data on human lysozyme 总被引:1,自引:0,他引:1
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Protease omega from Carica papaya L. has been purified and crystallized. The crystals are trigonal, space group P3(1)12 (or P3(2)12), with a = 7.42 +/- 0.02 nm, c = 7.79 +/- 0.02 nm with one molecule in the asymmetric unit. The crystals diffract to 0.19-nm resolution using synchrotron radiation. 相似文献
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G E Norris B F Anderson E N Baker H M Baker A L G?rtner J Ward S V Rumball 《Journal of molecular biology》1986,191(1):143-145
The purification of bovine lactoferrin, its crystallization at low ionic strength, and preliminary X-ray crystallographic data are reported. The crystals, which grow from a two-phase system, are radiation-stable and suitable for a medium-resolution X-ray analysis. They are orthorhombic, space group P2(1)2(1)2(1), with cell dimensions a = 138.4 A, b = 87.1 A, c = 73.6 A, and one protein molecule in the asymmetric unit. 相似文献