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1.
Sugar beets (Beta vulgaris L. cv. F58-554H1) were cultured hydroponically in growth chambers at 25°C, with a photon flux density of 500 mol m-2s-1. Measurements were made of net CO2 exchange, leaf adenylates (ATP, ADP and AMP), and leaf nicotinamide nucleotides (NAD+, NADP+, NADH, NADPH), over the diurnal period (16h light/8 h dark) and during photosynthetic induction. All the measurements were carried out on recently expanded leaves from 5-week-old plants. When the lights were switched on in the growth chamber, the rate of photosynthetic CO2 uptake, and the levels of leaf ATP and NADPH increased to a maximum in 30 min and remained there throughout the light period. The increase in ATP over the first few minutes of illumination was associated with the phosphorylation of ADP to ATP and the increase in NADPH with the reduction of NADP+; subsequently, the increase in ATP was associated with an increase in total adenylates while the increase in NADPH was associated with an accumulation of NADP+ and NADPH due to the light-driven phosphorylation of NAD+ to NADP+. On return to darkness, ATP and NADPH values decreased much more slowly, requiring 2 to 4 hours to reach minimum values. From these results we suggest that (i) the total adenylate and NADPH and NADP+ (but not NAD+ and NADH) pools increase following exposure to light; (ii) the increase in pool size is not accompanied by any large change in the energy or redox states of the system; and (iii) the measured ratios of ATP/ADP and NADPH/NADP+ for intact leaves are low and constant during steady-state illumination.Abbreviations AEC
adenylate energy charge
- DHAP
dihydroxyacetone phosphate
- MTT
3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide
- PES
phenazine ethosulfate
- PEP
phosphoenolpyruvate
- PGA
3-phosphoglycerate
- PFD
photon flux density
- Ru5P
ribulose-5-phosphate
- Rubisco
ribulose 1,5-bisphosphate carboxylase/oxygenase 相似文献
2.
The unicellular green algae Chlorella ellipsoidea was used tostudy transient changes in the energy state of adenylates andthe redox states of pyridine nucleotides induced by environmentalchanges. The transition from anaerobic to aerobic conditionsin the dark induced a sharp rise in the ATP ratio [ATP/(ATP+ADP+AMP)],a sudden decrease in the NADH ratio [NADH/(NAD++NADPH)] anda transient drop in the NADPH ratio [NADPH/(NADP++NADPH)]. Illuminationafter a dark period under anaerobic, CO2-free conditions inducedsharp increases in the ATP and NADPH ratios and a slower decreasein the NADH ratio. Illumination under aerobic conditions, ineither the presence or absence of CO2, caused a sharp increasein the NADPH ratio, a small increase in the ATP ratio and aslower increase in the NADH ratio. In the presence of CO2, asubsequent large drop in the NADPH ratio occurred. Darkeningunder anaerobic, CO2-free conditions induced a sudden decreasein the ATP ratio, a temporary fall in the NADPH ratio and aslow increase in the NADH ratio. Darkening under aerobic conditionsinduced transient drops in the ATP and NADPH ratios and a suddendrop in the NADH ratio. The addition of CO2 to the atmospherewith illumination produced a decrease in all three parameters. These results are discussed in relation to current theoriesof the interaction between photosynthesis and respiration. Ourobservations indicate that the energy and reducing potentialsgenerated by photochemical processes are used for and controlother processes besides CO2 fixation in photosynthetic cells. (Received December 3, 1981; Accepted May 4, 1982) 相似文献
3.
The light-driven, thiosulfate-dependent reduction of nicotinamideadenine dinucleotides under acrobic conditions in whole cellsof Chromatium vinosum was investigated. The total concentration of pyridine nucleotides in whole cellswas about 50 nmoles per µmole of bacteriochlorophyll.Under dark aerobic conditions, the majority of the nucleotidespresent was NAD+ with about 20% as NADP+. About 40% of the total NAD was reduced under continuous illumination.Thiosulfate or sulfide was needed for the photoreduction, whileorganic acids such as succinate or malate were not. The initialrate of NAD+ photoreduction in the presence of thiosulfate wasapproximately 100 nmoles per µmole of bacteriochlorophyllper min. The NAD+ photoreduction was strongly inhibited by uncouplersand electron transfer inhibitors. In contrast, an energy transferinhibitor, N, N'-dicyclohexylcarbodiimide, did not affect NAD+photoreduction at a concentration at which the light-inducedATP formation was inhibited. A transmembrane electrochemicalH+ gradient generated by cyclic electron transfer may be theenergy source for reduction of NAD+ in Chromatium vinosum. (Received April 2, 1980; ) 相似文献
4.
D Holten D Procsal H L Chang 《Biochemical and biophysical research communications》1976,68(2):436-441
Equilibrium dialysis indicates that rat liver glucose-6-P dehydrogenase binds two molecules of NADP+ per subunit with a dissociation constant of 0.6 × 10?6 M. The NADP+ free enzyme will not bind glucose-6-P indicating a compulsory order of substrate binding. Development of an isotopic assay allowed a direct measurement of the effect of physiological alterations in the NADP+/NADPH ratio on the activity of glucose-6-P and 6-phosphogluconate dehydrogenases. A combination of enzyme induction and altered NADP+/NADPH ratios could produce 30–50 fold changes in the capacity of these enzymes to produce NADPH during alterations in the nutritional state of the animal. 相似文献
5.
Cytosolic NADPH is the electron donor for extracellular fe reduction in iron-deficient bean roots
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Pyridine nucleotides were determined in lateral roots of iron-deficient and iron-sufficient Phaseolus vulgaris L. cv Prelude. In iron-deficient plants, total NADP per gram fresh weight and the NADPH/NADP+ ratio were twice the values found in iron-sufficient plants. The NADPH/NADP+ ratio in iron-deficient plants was considerably lowered after a 2 minute incubation in 1 millimolar ferricyanide. Total NAD was not influenced by growth conditions and was mainly present in oxidized form.
These results indicate that NADPH is the electron donor for the high FeIII reduction activity found in iron-deficient roots, a process that is part of the Fe-uptake mechanism.
相似文献6.
Liu YJ Norberg FE Szilágyi A De Paepe R Akerlund HE Rasmusson AG 《Plant & cell physiology》2008,49(2):251-263
Plant mitochondria contain alternative external NAD(P)H dehydrogenases,which oxidize cytosolic NADH or NADPH and reduce ubiquinonewithout inherent linkage to proton pumping and ATP production.In potato, St-NDB1 is an external Ca2+-dependent NADPH dehydrogenase.The physiological function of this enzyme was investigated inhomozygous Nicotiana sylvestris lines overexpressing St-ndb1and co-suppressing St-ndb1 and an N. sylvestris ndb1. In leafmitochondria isolated from the overexpressor lines, higher activityof alternative oxidase (AOX) was detected. However, the AOXinduction was substantially weaker than in the complex I-deficientCMSII mutant, previously shown to contain elevated amounts ofNAD(P)H dehydrogenases and AOX. An aox1b and an aox2 gene wereup-regulated in CMSII, but only aox1b showed a response, albeitsmaller, in the transgenic lines, indicating differences inAOX activation between the genotypes. As in CMSII, the increaseof AOX in the overexpressing lines was not due to a generaloxidative stress. The lines overexpressing St-ndb1 had consistentlylowered leaf NADPH/NADP+ ratios in the light and variably decreasedlevels in darkness, but unchanged NADH/NAD+ ratios. CMSII insteadhad similar NADPH/NADP+ and lower NADH/NAD+ ratios than thewild type. These results demonstrate that St-NDB1 is able tomodulate the cellular balance of NADPH and NADP+ at least inthe day and that reduction of NADP(H) and NAD(H) is independentlycontrolled. Similar growth rates, chloroplast malate dehydrogenaseactivation and xanthophyll ratios indicate that the change inreduction does not communicate to the chloroplast, and thatthe cell tolerates significant changes in NADP(H) reductionwithout deleterious effects. 相似文献
7.
L. Šindelár 《Biologia Plantarum》1986,28(6):440-448
The contents of NADP+, NADPH, changes in the activity of glucose-6-phosphate dehydrogenase and some questions relating to its regulation in leaf
tissues of tobacco plants infected with PVY were studied. The content of NADP+ and the total sum of pyridine nucleotides decreased after inoculation to 15 % and 30 %, respectively, whereas the content
of NADPH increased up to the threefold control value. The contents of NADP+, NADPH and Σ(NADPH + NADP+) linearly correlated with the reproduction curve of PVY. The value of the reduction charge RC and the value of the ratio
NADPH/NADP+ sharply increased after inoculation up to tenfold and eighteenfold values, respectively, of the healthy control.
The activity of glucose-6-phosphate dehydrogenase was markedly increased in virus-infected tissues during the entire experimental
period both in crude homogenate and after its partial purification when compared with the values found in healthy control
plants. The time courses of the activity curves of both crude and partially purified enzymes were coincident and correlated
with the reproduction curve of PVY. The results indicate the involvement of coarse regulation of the activity of the enzyme
by its content without the involvement of fine regulation by the ratio NADPH/NADP+ and RC. 相似文献
8.
Ferredoxin-Dependent Photoreduction of the Monodehydroascorbate Radical in Spinach Thylakoids 总被引:16,自引:0,他引:16
Thylakoid-bound and stromal ascorbate peroxidases scavenge thehydrogen peroxide that is photoproduced in PSI of chloroplastthylakoids. The primary oxidation product of ascorbate in thereaction catalyzed by ascorbate peroxidase, the monodehydroascorbate(MDA) radical, is photoreduced by thylakoids [Miyake and Asada(1992) Plant Cell Physiol. 33: 541]. We have now shown thatthe photoreduction of MDA radical in spinach thylakoids is largelydependent on ferredoxin (Fd), as determined by the monitoringthe MDA radical by electron paramagnetic resonance. Further,the reduced Fd generated by NADPH and Fd-NADP reductase couldreduce the MDA radical at a rate of over 106 M1 s1,indicating that the photoreduced Fd in PSI directly reducesthe MDA radical to ascorbate. Photoreduction of NADP+ by spinach thylakoids was suppressedby the MDA radical and conversely that of MDA radical was suppressedby NADP+, indicating a competition between the MDA radical andNADP+ for the photoreduced Fd in PSI. The ratio of the rateconstant for the photoreduction of MDA radical to that for thephotoreduction of NADP+ was estimated to be more than 30 to1. Thus, MDA radical is preferentially photoreduced as comparedto NADP+. From these results, we propose that the thylakoid-boundascorbate peroxidase and the Fd-dependent photoreduction ofMDA radical in PSI are the primary system for the scavengingof the hydrogen peroxide that is photoproduced in the thylakoids. (Received December 9, 1993; Accepted February 16, 1994) 相似文献
9.
Redox state of pyridine nucleotides of the endoplasmic reticulum (ER) lumen was determined in different nutritional conditions. NADPH-dependent cortisone reduction and NADP+-dependent cortisol oxidation were measured in rat liver microsomes, by utilizing the luminal 11β-hydroxysteroid dehydrogenase type 1 activity. Cortisone reduction decreased, while cortisol oxidation increased during onward starvation, showing that the luminal NADPH/NADP+ ratio was substantially decreased. Cortisone or metyrapone addition caused a smaller decrease in NADPH fluorescence in microsomes from starved rats. The results demonstrate that nutrient supply is mirrored by the redox state of ER luminal pyridine nucleotides. 相似文献
10.
Light intensity affects chlorophyll synthesis during greening process by metabolite signal from mitochondrial alternative oxidase in Arabidopsis 总被引:1,自引:0,他引:1
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Da‐Wei Zhang Shu Yuan Fei Xu Feng Zhu Ming Yuan Hua‐Xun Ye Hong‐Qing Guo Xin Lv Yanhai Yin Hong‐Hui Lin 《Plant, cell & environment》2016,39(1):12-25
Although mitochondrial alternative oxidase (AOX) has been proposed to play essential roles in high light stress tolerance, the effects of AOX on chlorophyll synthesis are unclear. Previous studies indicated that during greening, chlorophyll accumulation was largely delayed in plants whose mitochondrial cyanide‐resistant respiration was inhibited by knocking out nuclear encoded AOX gene. Here, we showed that this delay of chlorophyll accumulation was more significant under high light condition. Inhibition of cyanide‐resistant respiration was also accompanied by the increase of plastid NADPH/NADP+ ratio, especially under high light treatment which subsequently blocked the import of multiple plastidial proteins, such as some components of the photosynthetic electron transport chain, the Calvin–Benson cycle enzymes and malate/oxaloacetate shuttle components. Overexpression of AOX1a rescued the aox1a mutant phenotype, including the chlorophyll accumulation during greening and plastidial protein import. It thus suggests that light intensity affects chlorophyll synthesis during greening process by a metabolic signal, the AOX‐derived plastidial NADPH/NADP+ ratio change. Further, our results thus revealed a molecular mechanism of chloroplast–mitochondria interactions. 相似文献
11.
Mitochondria from the parasitic helminth, Hymenolepis diminuta, catalyzed both NADPH:NAD+ and NADH:NADP+ transhydrogenase reactions which were demonstrable employing the appropriate acetylpyridine nucleotide derivative as the hydride ion acceptor. Thionicotinamide NAD+ would not serve as the oxidant in the former reaction. Under the assay conditions employed, neither reaction was energy linked, and the NADPH:NAD+ system was approximately five times more active than the NADH:NADP+ system. The NADH:NADP+ reaction was inhibited by phosphate and imidazole buffers, EDTA, and adenyl nucleotides, while the NADPH:NAD+ reaction was inhibited only slightly by imidazole and unaffected by EDTA and adenyl nucleotides. Enzyme coupling techniques revealed that both transhydrogenase systems functioned when the appropriate physiological pyridine nucleotide was the hydride ion acceptor. An NADH:NAD+ transhydrogenase system, which was unaffected by EDTA, or adenyl nucleotides, also was demonstrable in the mitochondria of H. diminuta. Saturation kinetics indicated that the NADH:NAD+ reaction was the product of an independent enzyme system. Mitochondria derived from another parasitic helminth, Ascaris lumbricoides, catalyzed only a single transhydrogenase reaction, i.e., the NADH:NAD+ activity. Transhydrogenase systems from both parasites were essentially membrane bound and localized on the inner mitochondrial membrane. Physiologically, the NADPH:NAD+ transhydrogenase of H. diminuta may serve to couple the intramitochondrial metabolism of malate (via an NADP linked “malic” enzyme) to the anaerobic NADH-dependent ATP-generating fumarate reductase system. In A. lumbricoides, where the intramitochondrial metabolism of malate depends on an NAD-linked “malic” enzyme which is localized primarily in the intermembrane space, the NADH:NAD+ transhydrogenase activity may serve physiologically in the translocation of hydride ions across the inner membrane to the anaerobic energy-generating fumarate reductase system. 相似文献
12.
The Photoinhibition Site of Photosystem I in Isolated Chloroplasts under Extremely Reducing Conditions 总被引:4,自引:0,他引:4
Inoue Kazuhito; Fujii Tsutomu; Yokoyama Eiichi; Matsuura Katsumi; Hiyama Tetsuo; Sakurai Hidehiro 《Plant & cell physiology》1989,30(1):65-71
Under anaerobic atmosphere where the gas phase was simply replacedby N2, photo-inhibition of PS I of isolated spinach chloroplastswas insignificant. However, when dithionite was included inthe irradiation mixture, severe photoinhibition of the NADP+and the MV photo-reduction occurred. Neither P700 determinedby continuous illumination-induced difference spectroscopy,Fe-S centers determined by EPR under cryogenic temperatures,nor vitamin K-l determined by HPLC analysis were significantlydecreased under these photoinhibition conditions. Although photobleachingof antenna chlorophylls occurred to more or less extent, NADP+photoreduction activities were markedly depressed even undersaturating actinic light. The maximal amplitude of the flashinduced absorbance changes of P700 in ms range decreased almostin parallel with the loss of NADP+ photoreduction activity.These results indicate that although the Fe-S centers of thephotoinhibited chloroplasts were reducible by continuous illumination,to almost the same extents as that of the control chloroplasts,the efficiency of reduction by each flash was much lower thanthat of the control chloroplasts. The site of photoinhibitionin PS I under extremely reducing conditions is between A0 andFe-S X. (Received July 28, 1988; Accepted October 31, 1988) 相似文献
13.
The NAD+/NADH ratio was 12 in whole soybean nodules tissue,but only 2 in bacteroids, as a result of the high concentrationof NADH. By contrast, NADP+/NADPH ratios were less than unityin both nodules and bacteroids, being 0.28 and 0.37, respectively. The adenylate energy charge values in bacteroids and nodules,0.37 and 0.39, respectively, were remarkably low, and were insharp contrast to the normal value of 0.83 in root tissue. (Received July 19, 1988; Accepted March 9, 1989) 相似文献
14.
A sonicate of Achromobacter parvulus IFO-13182 produced NADPH from NADP+by an NADP+-linked malic enzyme [l-malate: NAD(P)+oxidoreductase, EC 1.1.1.39–40] reaction in the presence of l-malic acid and divalent metal ions. Malic enzyme of A. parvulus was stabilized by 5% l-malic acid, and activity was maintained at 60°C for 1 h. Contaminating phosphatase (orthophosphoricmonoester phosphohydrolase, EC 3.1.3.1–2) was completely inactivated by this treatment. Among the conditions tested, the optimum NADPH production was done using 36 μmol NADP+, 67 μmol l-malic acid, 63 μmol MgCl2 and 1 unit of the malic enzyme in 3 ml of 55 mm phosphate buffer (pH 7.8). Conversion ratio of NADPH from NADP+ reached 100% after 4 h incubation at 30°C and the amount of NADPH accumulated was ~12 μmol ml?1of the reaction mixture. No dephosphorylation of NADP+to NAD+or of NADPH to NADH was found by high performance liquid chromatography. The NADPH produced by such enzymatic reduction was purified by ethanol precipitation and dried in vacuo in powdered form with 97% purity, judged from the ratio of the absorbances at 340 and 260 nm. The purity of the NADPH produced was determined to be 95% from its coenzyme activity with NAD(P)+-linked glutathione reductase [NAD(P)H: oxidized-glutathione oxidoreductase, EC 1.6.4.2]. 相似文献
15.
NADP+-isocitrate dehydrogenase (threo-DS-isocitrate: NADP+ oxidoreductase [decarboxylating]; EC 1.1.1.42) (IDH) from pod walls of chickpea (Cicer arietinum L.) was purified 192-fold using ammonium sulfate fractionation, ion exchange chromatography on DEAE-Sephadex A-50, and gel filtration through Sephadex G-200. The purified enzyme, having a molecular weight of about 126,000, exhibited a broad pH optima from 8.0 to 8.6. It was quite stable at 4°C and had an absolute requirement for a divalent cation, either Mg2+ or Mn2+, for its activity. Typical hyperbolic kinetics was obtained with increasing concentrations of NADP+, dl-isocitrate, Mn2+, and Mg2+. Their Km values were 15, 110, 15, and 192 micromolar, respectively. The enzyme activity was inhibited by sulfhydryl reagents. Various amino acids, amides, organic acids, nucleotides, each at a concentration of 5 millimolar, had no effect on the activity of the enzyme. The activity was not influenced by adenylate energy charge but decreased linearly with increasing ratio of NADPH to NADP+. Initial velocity studies indicated kinetic mechanism to be sequential. NADPH inhibited the forward reaction competitively with respect to NADP+ at fixed saturating concentration of isocitrate, whereas 2-oxoglutarate inhibited the enzyme noncompetitively at saturating concentrations of both NADP+ and isocitrate, indicating the reaction mechanism to be random sequential. Results suggest that the activity of NADP+-IDH in situ is likely to be controlled by intracellular NADPH to NADP+ ratio as well as by the concentration of various substrates and products. 相似文献
16.
Here, we report that the smooth muscle and endothelium of the pig coronary artery differ in the profiles of energy metabolism nucleotides. ATP levels in the freshly isolated smooth muscle (1490 ± 93, all the values are in pmol/mg protein) were significantly greater than in the endothelium (418 ± 68). In contrast, endothelium contained higher levels of NADH (328 ± 21), NAD+ (1210 ± 28), NADPH (87 ± 2), and NADP+ (77 ± 4) than smooth muscle (17 ± 2, 96 ±14, 7 ± 1, and 8 ± 1, respectively). However, smooth muscle and endothelium do not differ from each other in the ratios of NADH/NAD+ or NADPH/NADP+. Cells cultured from smooth muscle and endothelium contained less ATP (93 ± 2, 141 ± 6) and had lower ratios of NADH/NAD+ than the freshly isolated tissues but the NADPH/NADP+ ratios remained similar. We conclude that (a) freshly isolated smooth muscle and endothelium differ in their profiles of the energy metabolism nucleotides, and (b) culturing the cells alters the profile. 相似文献
17.
Holfgrefe Simone; Backhausen Jan E.; Kitzmann Camillo; Scheibe Renate 《Plant & cell physiology》1997,38(11):1207-1216
The reactions of isolated intact spinach chloroplasts at saturatinglight and CO2 to changes in steady-state electron flow werefollowed at the various stages of photosynthesis. Alterationsin the rate of electron flow were induced by the addition ofoxaloacetate (OAA), nitrite or methyl viologen (MV). Two typesof effect can be distinguished: (1) When a small fraction ofthe electrons produced are accepted by OAA or nitrite (up to20% of the electrons produced in the light), the activationstate of the NADP+-dependent malate dehydrogenase (NADP-MDH)was strongly decreased, whereas qP and the rate of O2-productionwere increased. qN, the stromal metabolite pools and the [14C]-CO2-fixationrate were only marginally influenced. (2) Higher amounts ofnitrite or MV decreased O2 production and strongly inhibited[14C]CO2 fixation. This treatment further increased the ATP/ADPratio, but had little effect on the NADPH + H+/NADP+ ratio.The stromal concentrations of 3PGA, DHAP and FBP, and the ratesof 3PGA and DHAP export were drastically changed. In particular,the DHAP/3PGA ratio increased, and the rate of 3PGA export wasdecreased by minor changes in the rate of electron flow. Additionof high amounts of nitrite or MV, but not of OAA decreased theactivation states of NADP-MDH and fructose 1,6-bisphosphatase(FBPase), while the activation states of NADP+-dependent glyceraldehyde3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK)remained unchanged under all conditions. (Received February 10, 1997; Accepted September 2, 1997) 相似文献
18.
R.Kenneth Burkhard 《Archives of biochemistry and biophysics》1982,218(1):207-212
Association constants were determined for the 1:1 interactions of calcium with NAD+, NADH, NADP+, and NADPH in aqueous systems (pH 7, 25 °C) by use of a calcium-sensitive electrode. The order of binding of calcium to these pyridine nucleotides appears to be NAD+ < NADH < NADP+ < NADPH with association constants of 0.2 × 102, 0.3 × 102, 0.9 × 102, and 2 × 102, respectively. Calorimetric experiments revealed that all of these interactions are endothermic with enthalpy changes of 1, 2, 2, and 3 kcal/mol, respectively. 相似文献
19.
Inokuchi Ritsuko; Itagaki Tadashi; Wiskich Joseph T.; Nakayama Katsumi; Okada Mitsumasa 《Plant & cell physiology》1997,38(3):327-335
NADP-glutamate dehydrogenase (EC 1.4.1.4
[EC]
; NADP-GDH) was purifiedto electrophoretic homogeneity from the multinuclear-unicellulargreen marine alga in Sipho-nales, Bryopsis maxima, and its propertieswere examined. Mr of the undenatured enzyme was 280 kDa, andthe enzyme is thought to be a hexamer of 46 kDa subunit protein.Optimum pHs for the reductive amination and oxidative deaminationwere 7.5 and 8.2-9.0 respectively. The enzyme displayed NADPH/NADH-specificactivities with a ratio of 18 :1. Apparent Km values for 2-oxoglutarate,ammonia, NADPH, glutamate and NADP+ were 3.0, 2.2, 0.03, 3.2and 0.01 mM respectively. The enzymochemical characteristicsof the GDH were studied and compared to those of other species.The B. maxima GDH was insensitive to 5 mM Ca2+ and to 1 mM EDTAin contrast to higher plant NAD-GDHs. Chemical modificationswith DTNB and pCMBS suggested that cysteine residues are essentialfor the enzymatic activity as in other species GDHs. The GDHwas not affected by 1 mM purine nucleotides, suggesting thatthe enzyme is not allosteric, in contrast to animal NAD(P)-GDHsand fungal NAD-GDHs. (Received August 12, 1996; Accepted January 7, 1997) 相似文献
20.
The activity of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) from spinach chloroplasts is strongly regulated by the ratio of NADPH/NADP+, with the extent of this regulation controlled by the concentration of ribulose 1,5-diphosphate. Other metabolites of the reductive pentose phosphate cycle are far less effective in mediating the regulation of the enzyme activity by NADPH/NADP+ ratio. With a ratio of NADPH/NADP+ of 2, and a concentration of ribulose 1,5-diphosphate of 0.6 mM, the activity of the enzyme is completely inhibited.This level of ribulose 1,5-diphosphate is well within the concentration range which has been reported for unicellular green algae photosynthesizing in vivo. Ratios of NADPH/NADP+ of 2.0 have been measured for isolated spinach chloroplasts in the light and under physiological conditions.Since ribulose 1,5-diphosphate is a metabolite unique to the reductive pentose phosphate cycle and inhibits glucose-6-phosphate dehydrogenase in the presence of NADPH/NADP+ ratios found in chloroplasts in the light, it is proposed that regulation of the oxidative pentose phosphate cycle is accomplished in vivo by the levels of ribulose 1,5-diphosphate, NADPH, and NADP+.It already has been shown that several key reactions of the reductive pentose phosphate cycle in chloroplasts are regulated by levels of NADPH/NADP+ or other electron-carrying cofactors, and at least one key-regulated step, the carboxylation reaction is strongly affected by 6-phosphogluconate, the metabolite unique to the oxidative pentose phosphate cycle. Thus there is an interesting inverse regulation system in chloroplasts, in which reduced/oxidized coenzymes provide a general regulatory mechanism. The reductive cycle is activated at high NADPH/NADP+ ratios where the oxidative cycle is inhibited, and ribulose 1,5-diphosphate and 6-phosphogluconate provide further control of the cycles, each regulating the cycle in which it is not a metabolite. 相似文献