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1.
Excitation spectra of chlorophyll a fluorescence in chloroplasts from spinach and barley were measured at 4.2 K. The spectra showed about the same resolution as the corresponding absorption spectra. Excitation spectra for long-wave chlorophyll a emission (738 or 733 nm) indicate that the main absorption maximum of the photosystem (PS) I complex is at 680 nm, with minor bands at longer wavelengths. From the corresponding excitation spectra it was concluded that the emission bands at 686 and 695 nm both originate from the PS II complex. The main absorption bands of this complex were at 676 and 684 nm. The PS I and PS II excitation spectra both showed a contribution by the light-harvesting chlorophyll protein(s), but direct energy transfer from PS II to PS I was not observed at 4 K. Omission of Mg 2+ from the suspension favored energy transfer from the light-harvesting protein to PS I. Excitation spectra of a chlorophyll b-less mutant of barley showed an average efficiency of 50–60% for energy transfer from β-carotene to chlorophyll a in the PS I and in the PS II complexes. 相似文献
2.
The membrane-bound pigment system of green sulfur bacteria consists of light-harvesting bacteriochlorophyll a-protein and a ‘core complex’ that is associated with the reaction center (Kramer, H.J.M., Kingma, H., Swarthoff, T. and Amesz, J. (1982) Biochim. Biophys. Acta 681, 359–364). The isolation and properties of the core complex from Prosthecochloris aestuarii are described. The complex has a molecular mass of 200 ± 50 kDa and contains bacteriochlorophyll a, carotenoid and pigments absorbing near 670 nm (probably bacteriopheophytin c and an unidentified pigment). Fluorescence emission spectra and sodium dodecyl sulfate polyacrylamide gel electrophoresis showed the absence of light-harvesting bacteriochlorophyll a-protein. The preparation showed no reaction center activity. Circular and linear dichroism spectra indicated that the structure of the core complex was basically not altered by the isolation procedure. Comparison with the CD spectrum of the intrinsic membrane-bound pigment-protein complex indicates that the latter contains 14 bacteriochlorophyll a molecules (two subunits) belonging to the light-harvesting protein and about 20 bacteriochlorophyll a molecules belonging to the core complex. 相似文献
3.
Green sulfur bacteria possess a complex photosynthetic machinery. The dominant light harvesting systems are chlorosomes, which consist of bacteriochlorophyll c, d or e oligomers with small amounts of protein. The chlorosomes are energetically coupled to the membrane-embedded iron sulfur-type reaction center via a bacteriochlorophyll a-containing baseplate protein and the Fenna-Matthews-Olson (FMO) antenna protein. The fluorescence yield and spectral properties of these photosynthetic complexes were investigated in intact cells of several species of green sulfur bacteria under physiological, anaerobic conditions. Surprisingly, green sulfur bacteria show a complex modulation of fluorescence yield upon illumination that is very similar to that observed in oxygenic phototrophs. Within a few seconds of illumination, the fluorescence reaches a maximum, which decreases within a minute of illumination to a lower steady state. Fluorescence spectroscopy reveals that the fluorescence yield during both processes is primarily modulated on the FMO-protein level, while the emission from chlorosomes remains mostly unchanged. The two most likely candidates that modulate bacteriochlorophyll fluorescence are (1) direct excitation quenching at the FMO-protein level and (2) indirect modulation of FMO-protein fluorescence by the reduction state of electron carriers that are part of the reaction center. 相似文献
4.
Whole cells and isolated chlorosomes (antenna complex) of the green photosynthetic bacterium Chloroflexus aurantiacus have been studied by absorption spectroscopy (77 K and room temperature), fluorescence spectroscopy, circular dichroism, linear dichroism and electron spin resonance spectroscopy. The chlorosome absorption spectrum has maxima at 450 (contributed by carotenoids and bacteriochlorophyll (BChl) a Soret), 742 (BChl c) and 792 nm (BChl a) with intensity ratios of 20:25. The fluorescence emission spectrum has peaks at 748 and 802 nm when excitation is into either the 742 or 450 nm absorption bands, respectively. Whole cells have fluorescence peaks identical to those in chlorosomes with the addition of a major peak observed at 867 nm. The CD spectrum of isolated chlorosomes has an asymmetric-derivative-shaped CD centered at 739 nm suggestive of exciton interaction at least on the level of dimers. Linear dichroism of oriented chlorosomes shows preferential absorption at 742 nm of light polarized parallel to the long axis of the chlorosome. This implies that the transition dipoles are also oriented more or less parallel to the long axis of the chlorosome. Treatment with ferricyanide results in the appearance of a 2.3 G wide ESR spectrum at g 2.002. Whole cells grown under different light conditions exhibit different fluorescence behavior when absorption is normalized at 742 nm. Cells grown under low light conditions have higher fluorescence intensity at 748 nm and lower intensity at 802 nm than cells grown under high light conditions. These results indicate that the BChl c in chlorosomes is highly organized, and transfers energy from BChl c (742 nm) to a connector of baseplate BChl B792 (BChl a) presumably located in the chlorosome baseplate adjacent to the cytoplasmic membrane. 相似文献
5.
Isolated chlorosomes of the photosynthetic green sulfur bacterium Chorobium limicola upon cooling to 4 K showed, in addition to the near-infrared absorption band at 753 nm due to bacteriochlorophyll c, a weak band near 800 nm that could be attributed to bacteriochlorophyll a. The emission spectrum showed bands of bacteriochlorophyll c and a at 788 and 828 nm, respectively. The fluorescence excitation spectrum indicated a high efficiency of energy transfer from bacteriochlorophyll c to bacteriochlorophyll a. When all bacteriochlorophyll c absorption had been lost upon storage, no appreciable change in the optical properties of the bacteriochlorophyll a contained in these ‘depleted chlorosomes’ was observed. The fluorescence and absorption spectra of the chlorosomal bacteriochlorophyll a were clearly different from those of the soluble bacteriochlorophyll a protein present in these bacteria. The results provide strong evidence that bacteriochlorophyll a, although present in a small amount, is an integral constituent of the chlorosome. It presumably functions in the transfer of energy from the chlorosome to the photosynthetic membrane; its spectral properties and the orientation of its near-infrared optical transitions as determined by linear dichroism are such as to favor this energy transfer. 相似文献
6.
The orientation of pigments and pigment-protein complexes of the green photosynthetic bacterium Prosthecochloris aestuarii was studied by measurement of linear dichroism spectra at 295 and 100 K. Orientation of intact cells and membrane vesicles (Complex I) was obtained by drying on a glass plate. The photochemically active pigment-protein complexes (photosystem-protein complex and reaction center pigment-protein complex) and the antenna bacteriochlorophyll a protein were oriented by pressing a polyacrylamide gel. The data indicate that the near-infrared transitions (Q y) of bacteriochlorophyll c and most bacteriochlorophyll a molecules have a relatively parallel orientation to the membrane, whereas the Q y transitions of the bacteriochlorophyll a in the antenna protein are oriented predominantly perpendicularly to the membrane. Carotenoids and the Q x transitions (590–620 nm) of bacteriochlorophyll a, not belonging to the bacteriochlorophyll a protein, have a relatively perpendicular orientation to the membrane. The absorption and linear dichroism spectra indicate the existence of different pools of bacteriochlorophyll c in the chlorosomes and of carotenoid and bacteriopheophytin c in the cell membrane. The results suggest that the photosystem-protein and reaction center pigment-protein complexes are oriented with their short axes approximately perpendicular to the plane of the membrane. The symmetry axis of the bacteriochlorophyll a protein has an approximately perpendicular orientation. 相似文献
7.
1. The excitation spectrum for the bacteriochlorophyll P890 fluorescence in reaction centre preparations was determined at wavelengths ranging from 360 to 890 nm.2. A fluorescence excitation spectrum corresponding to the absorbance spectrum of bacteriopheophytin was also obtained. This spectrum was used in an analysis of the absorbance spectrum of a reaction centre preparation. Based on this spectrum and on literature data, we estimated that the bacteriopheophytin: bacteriochlorophyll ratio in reaction centre particles is at least 1 : 2.3. On the basis of literature data, it is shown that bacteriopheophytin occurs probably as such in reaction centres in vivo. 相似文献
8.
Emission spectra of bacteriochlorophyll a fluorescence and absorption spectra of various purple bacteria were measured at temperatures between 295 and 4 K. For Rhodospirillum rubrum the relative yield of photochemistry was measured in the same temperature region. In agreement with earlier results, sharpening and shifts of absorption bands were observed upon cooling to 77 K. Below 77 K further sharpening occurred. In all species an absorption band was observed at 751-757 nm. The position of this band and its amplitude relative to the concentration of reaction centers indicate that this band is due to reaction center bacteriopheophytin. The main infrared absorption band of Rhodopseudomonas sphaeroides strain R26 is resolved in two bands at low temperature, which may suggest that there are two pigment-protein complexes in this species. Emission bands, like the absorption bands, shifted and sharpened upon cooling. The fluorescence yield remained constant or even decreased in some species between room temperature and 120 K, but showed an increased below 120 K. This increase was most pronounced in species, such as R. rubrum, which showed single banded emission spectra. In Chromatium vinosum three (835, 893 and 934 nm) and in Rps. sphaeroides two (888 and 909 nm) emission bands were observed at low temperature. The temperature dependence of the amplitudes of the short wavelength bands indicated the absence of a thermal equilibrium for the excitation energy distribution in C. vinosum and Rps. sphaeroides. In all species the increased in the yield was larger when all reaction centers were photochemically active than when the reaction centers were closed. In R. rubrum the increase in the fluorescence yield was accompanied by a decrease of the quantum yield of charge separation upon excitation of the antenna but not of the reaction center chlorophyll. Calculation of the F?rster resonance integral at various temperatures indicated that the increase in fluorescence yield and the decrease in the yield of photochemistry may be due to a decrease in the rate of energy transfer between antenna bacteriochlorophyll molecules. The energy transfer from carotenoids to bacteriochlorophyll was independent of the temperature in all species examined. The results are discussed in terms of existing models for energy transfer in the antenna pigment system. 相似文献
9.
Changes in low-temperature fluorescence spectra of pea chloroplasts induced by the short-term heating were studied. Excitation spectra of the long-wavelength fluorescence were studied as well. Heating was carried out at 45°C for 5 min in the darkness or in the presence of white light sourced with intensities of 260 or 1400 μmol/m 2 s. All variants of heating decreased the intensity of the long-wavelength fluorescence band. The integral of the excitation spectrum decreased after the exposure to heating in the darkness and increased after the exposure to heating in the presence of light. The observed changes in most intensive components — 726, 729 and 731 nm — of the long-wavelength fluorescence band, induced by various modes of heating, were similar. The changes in the fourth intensive component at 735 nm were different. Twenty-five components were found in the fine structure of the excitation spectrum of the long-wavelength fluorescence. Positions of most of peaks corresponded to the absorption peaks of Lhca proteins. Heat-induced changes in the excitation spectrum in the regions corresponding to the absorption of chl b and short-wavelength forms of chl a have been shown to correlate with changes in the intensities of the 726-, 729-, and 731-nm components of the long-wavelength fluorescence. This allows one to assign them to the emission of the outer antenna of Photosystem I. Changes in the intensity of the component at 735 nm correlated only with changes in excitation spectrum in the long-wavelength region that corresponded to the absorption of the long-wave-length forms of chlorophyll a. Therefore, the 735-nm component could be assigned to the emission of the Photosystem I inner antenna. Analysis of the changes induced by heating in the emission and excitation spectra of fluorescence revealed changes in the energy transfer in the outer and the inner antennas of Photosystem I. Heating in the darkness lowered the energy transfer in the outer and in the inner antennas. Both modes of heating in the presence of light increased the energy transfer in the outer antenna. For the inner antenna, presence of the light promotes an efficient of energy transfer at the levels close to the control one. It is proposed that illumination during heating exposure causes a specific state of the antenna complex in Photosystem I that provides an increase in funneling of the energy toward the reaction centers. 相似文献
10.
This paper presents a concise review of the structural factors which control the energy of the Q y absorption band of bacteriochlorophyll a in purple bacterial antenna complexes. The energy of these Q y absorption bands is important for excitation energy transfer within the bacterial photosynthetic unit.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
11.
The magnetic field effects on bacteriochlorophyll fluorescence in six strains of Rhodopseudomonas capsulata were investigated. All strains exhibit an increase in fluorescence upon application of a magnetic field. Large magnetic field effects are shown to arise in mutants which contain the B800–850 complex as the only bacteriochlorophyll-containing protein. These fluorescence increases are observed only with carotenoid excitation and are best described by a carotenoid singlet heterofission mechanism. Variations in the magnitudes of the magnetic field effects for the Rps. capsulata strain arise from energy differences in the excited states of the molecules involved in the process. In order to determine the contribution from reaction centers to the magnetic field effects observed in the mutants which contain all three pigment-protein complexes, reaction centers were isolated from these strains. The reaction center contribution to the magnetic field effect on fluorescence in whole cells was determined to be smaller than the antenna contribution when carotenoid excitation was employed. 相似文献
12.
We have studied the pigment arrangement in purified cytoplasmic membranes of the thermophilic green bacterium Chloroflexus aurantiacus. The membranes contain 30–35 antenna bacteriochlorophyll a molecules per reaction center; these are organized in the B808–866 light-harvesting complex, together with carotenoids in a 2:1 molar ratio. Measurements of linear dichroism in a pressed polyacrylamide gel permitted the accurate determination of the orientation of the optical transition dipole moments with respect to the membrane plane. Combination of linear dichroism and low temperature fluorescence polarization data shows that the Q y transitions of the BChl 866 molecules all lie almost perfectly parallel to the membrane plane, but have no preferred orientation within the plane. The BChl 808 Q y transitions make an average angle of about 44° with this plane. This demonstrates that there are clear structural differences between the B808–866 complex of C. aurantiacus and the B800–850 complex of purple bacteria. Excitation energy transfer from carotenoid to BChl a proceeds with about 40% efficiency, while the efficiency of energy transfer from BChl 808 to BChl 866 approaches 100%. From the minimal energy transfer rate between the two spectral forms of BChl a, obtained by analysis of low temperature fluorescence emission spectra, a maximal distance between BChl 808 and BChl 866 of 23 was derived.Abbreviations BChl
bacteriochlorophyll
- BPheo
bacteriopheophytin
- CD
circular dichroism
- LD
linear dichroism
- Tris
Tris(hydroxymethyl)aminomethane 相似文献
13.
Picosecond absorbance difference spectra at a number of delay times after a 35 ps excitation pulse and kinetics of absorbance changes were measured in chromatophores of the photosynthetic purple bacterium Rhodospirillum rubrum after chemical oxidation of the primary electron donor P-875. Kinetics and spectra were measured of the excited singlet states of carotenoid and bacteriochlorophyll a and also of the triplet state of the carotenoid. The excited singlet state of carotenoid, produced by direct excitation at 532 nm, is characterized by a bleaching of the ground state absorption bands in the region 450–490 nm and by an absorbance increase with a maximum near 570 nm. Its lifetime was calculated to be 0.6 ± 0.1 ps in vitro and less than 1 ps in vivo. The triplet state of carotenoid in vivo is formed within 100 ps after direct carotenoid excitation via a pathway that does not involve excited states of bacteriochlorophyll. Singlet excitation of a bacteriochlorophyll a molecule causes the bleaching of its Q x and Q y absorption bands, and is probably associated with blue shifts of the Q y absorption band of about six neighboring bacteriochlorophyll molecules. Upon increasing the excitation density, the average lifetime of the singlet excitations on bacteriochlorophyll decreased from about 350 ps to about 10 ps or less. The results are in quantitative agreement with the known effect of singlet-singlet annihilation upon the fluorescence yield, and furthermore show that no bacteriochlorophyll or carotenoid triplet formation is associated with this annihilation. 相似文献
14.
Emission and absorption spectra in the temperature range 4–300 K have been obtained for bacteriochlorophyll light-harvesting complexes (B800–850 complexes) from several mutants of Rhodopseudomonas sphaeroides and a nonphotosynthetic mutant of Rhodopseudomonas capsulata. The energy-transfer properties of these complexes were remarkably similar despite differences in carotenoid composition. Between 300 and 200 K the excitation densities in B800 and B850 are in thermal equilibrium, indicating rapid energy transfer from B800 to B850 and vice versa. The temperature dependence of the ratio of the B800 and B850 emission yields allows the determination of the ratio of the number of B800 and B850 molecules in the complex which is close to 0.5. Below 200 K thermal equilibrium no longer exists. At 4–100 K the B800 emission yield increases with decreasing temperature and becomes dependent on the wavelength of excitation. From the B800 emission yield at 4 K the B800–850 dipole-dipole distance was calculated to be equal to or smaller than 21 Å for all B800–850 complexes. Excitation spectra for B800 and B850 emission show that the overall energy-transfer efficiencies from carotenoid and B800 to B850 are greater than 90% at all temperatures. At 4 K the carotenoid transfers its excitation energy preferentially to B850. Experiments with chromatophores indicated that the energy-transfer properties of the B800–850 complexes were not modified by the isolation procedures. 相似文献
15.
Chromatophores from photosynthetic bacteria were excited with flashes lasting approx. 15 ns. Transient optical absorbance changes not associated with the photochemical electron-transfer reactions were interpreted as reflecting the conversion of bacteriochlorophyll or carotenoids into triplet states. Triplet states of various carotenoids were detected in five strains of bacteria; triplet states of bacteriochlorophyll, in two strains that lack carotenoids. Triplet states of antenna pigments could be distinguished from those of pigments specifically associated with the photochemical reaction centers. Antenna pigments were converted into their triplet states if the photochemical apparatus was oversaturated with light, if the primary photochemical reaction was blocked by prior chemical oxidation of P-870 or reduction of the primary electron acceptor, or if the bacteria were genetically devoid of reaction centers. Only the reduction of the electron acceptor appeared to lead to the formation of triplet states in the reaction centers.In the antenna bacteriochlorophyll, triplet states probably arise from excited singlet states by intersystem crossing. The antenna carotenoid triplets probably are formed by energy transfer from triplet antenna bacteriochlorophyll. The energy transfer process has a half time of approx. 20 ns, and is about 1 × 10 3 times more rapid than the reaction of the bacteriochlorophyll triplet states with O 2. This is consistent with a role of carotenoids in preventing the formation of singlet O 2 in vivo. In the absence of carotenoids and O 2, the decay half times of the triplet states are 70 μs for the antenna bacteriochlorophyll and 6–10 μs for the reaction center bacteriochlorophyll. The carotenoid triplets decay with half times of 2–8 μs.With weak flashes, the quantum yields of the antenna triplet states are in the order of 0.02. The quantum yields decline severely after approximately one triplet state is formed per photosynthetic unit, so that even extremely strong flashes convert only a very small fraction of the antenna pigments into triplet states. The yield of fluorescence from the antenna bacteriochlorophyll declines similarly. These observations can be explained by the proposal that singlet-triplet fusion causes rapid quenching of excited singlet states in the antenna bacteriochlorophyll. 相似文献
16.
The three-dimensional structure of a water-soluble bacteriochlorophyll a-containing protein from the green photosynthetic bacterium Prosthecochloris aestuarii has been determined by X-ray crystallography from a 2.8 Å resolution electron density map based on four isomorphous derivatives. Details of the crystallographic procedures used to obtain the map are presented.The bacteriochlorophyll a-protein is shown to consist of three identical subunits, tightly packed around a 3-fold symmetry axis. Each subunit consists of a core of seven bacteriochlorophyll a molecules enclosed within a “bag” of protein. The polypeptide chain forms an extensive 15-strand β-sheet, which is almost planar in its central region, and twisted at its extremities, and wraps around the chlorophyll core to form an efficient amphipathic layer between the chlorophylls and the aqueous environment. There are extensive contacts between the phytyl chains of the seven bacteriochlorophylls within each subunit. These hydrocarbon chains constitute an inner hydrophobic core of the molecule which may be important in forming the complex. There are also extensive contacts between the protein and both the bacteriochlorophyll head groups and tails, but relatively few contacts between the respective head groups. The seven magnesiums all appear to be five co-ordinated. In five cases the presumed ligand is a histidine side-chain, in one case a polypeptide carbonyl oxygen, and in the other case a water molecule.At low temperature, both the absorption and circular dichroism spectra of the bacteriochlorophyll a-protein show splitting which can be interpreted in general terms as due to exciton interactions between the seven chromophores, but calculations of the expected splitting based on the bacteriochlorophyll co-ordinates determined crystallographically are in poor agreement with the observed spectra. Furthermore, the observed red shift of the Q y absorption band of bacteriochlorophyll a, from about 770 nm in organic solvents to 809 nm in the bacteriochlorophyll a-protein, is not explained by the exciton calculations. It seems likely that the red shift is due to perturbations of the spectra of the individual bacteriochlorophylls by the protein environment, but, pending the determination of the amino acid sequence, it is not possible at this time to define in detail all the proteinchlorophyll interactions. It is suggested that the bacteriochlorophyll a-protein serves as a good model for the organization of chlorophyll in vivo, and that the types of interaction seen here between chlorophyll and protein are likely to be found in other chlorophyll proteins. 相似文献
17.
The picosecond time-domain incoherent singlet excitation transfer and trapping kinetics in core antenna of photosynthetic bacteria are studied in case of low excitation intensities by numerical integration of the appropriate master equation in a wide temperature range of 4-300 K. The essential features of our two-dimensional-lattice model are as follows: Förster excitation transfer theory, spectral heterogeneity of both the light-harvesting antenna and the reaction center, treatment of temperature effects through temperature dependence of spectral bands, inclusion of inner structure of the trap, and transition dipole moment orientation. The fluorescence kinetics is analyzed in terms of distributions of various kinetic components, and the influence of different inhomogeneities (orientational, spectral) is studied. A reasonably good agreement between theoretical and experimental fluorescence decay kinetics for purple photosynthetic bacterium Rhodospirillum rubrum is achieved at high temperatures by assuming relatively large antenna spectral inhomogeneity: 20 nm at the whole bandwidth of 40 nm. The mean residence time in the antenna lattice site (it is assumed to be the aggregate of four bacteriochlorophyll a molecules bound to proteins) is estimated to be ~12 ps. At 4 K only qualitative agreement between model and experiment is gained. The failure of quantitative fitting is perhaps due to the lack of knowledge about the real structure of antenna or local heating and cooling effects not taken into account. 相似文献
18.
Picosecond absorbance difference spectra at a number of delay times after a 35 ps excitation flash and kinetics of absorbance changes were measured of the membrane vesicle preparation Complex I from the photosynthetic green sulfur bacterium Prosthecochloris aestuarii. After chemical oxidation of the primary donor the excitation pulse produced singlet and triplet excited states of carotenoid and bacteriochlorophyll a. With active reaction centers present also the flash-induced primary charge separation and subsequent electron transfer were observed. The singlet excited state of the carotenoid, formed by direct excitation at 532 nm, is characterized by an absorbance band peaking at 590 nm. Its average lifetime was calculated to be about 1 ps. Excited singlet states of bacteriochlorophyll a were characterized by a bleaching of their ground state Q y absorption bands. Singlet excited states, localized on the so-called core complex, were produced by energy transfer from excited carotenoid. Their lifetime was about 70 ps. A decay component of about 280 ps was ascribed to singlet excited bacteriochlorophyll a in the bacteriochlorophyll a protein. These singlet excitations were partly converted to the triplet state. With active reaction centers, oxidation of the primary donor, P-840, characterized by the bleaching of its Q y and Q x absorption bands, was observed. This oxidation was accompanied by a bleaching between 650 and 680 nm and an absorbance increase between 680 and 750 nm. These changes, presumably due to reduction of bacteriopheophytin c (Van Bochove, A.C., Swarthoff, T., Kingma, H., Hof, R.M., Van Grondelle, R., Duysens, L.N.M. and Amesz, J. (1984) Biochim. Biophys. Acta 764, 343–346), were attributed to the reduction of the primary electron acceptor. Electron transfer to a secondary acceptor occurred with a time-constant of 550 ± 50 ps. Since no absorbance changes due to reduction of this acceptor were observed in the red or infrared region, we tentatively assume that this acceptor is an iron-sulfur center. 相似文献
19.
The isolation of two native light harvesting bacteriochlorophyl · protein complexes from Rhodopseudomonas capsulata is described. The light harvesting bacteriochlorophyll I (B 875) has been isolated from the blue-green mutant Ala + lacking both carotenoids and light harvesting bacteriochlorophyll II. Light harvesting bacteriochlorophyll I is associated with a protein (light harvesting band 2) of 12 000 molecular weight.Light harvesting bacteriochlorophyll II complex has been isolated from the mutant Y5 lacking a reaction center and light harvesting bacteriochlorophyll I. Light harvesting bacteriochlorphyll II (B 800 + 850) together with carotenoids is associated with two polypeptides (light harvesting bands 3 and 4) having molecular weights of about 8000 and 10 000 (sodium dodecyl sulfate polyacrylamide gel electrophoresis). A third protein (light harvesting band 1) is in the purified light harvesting II fraction (mol. wt. approx. 14 000), but not associated with bacteriochlorophyll or carotenoids. The amino acid composition of the 3 antenna pigment II proteins is given. The polarity of these proteins was found to be 48%. From the amino acid composition the following molecular weights were calculated band 1: 17 350, band 3: 13 350 and band 4: 10 500. 相似文献
20.
The transfer of excitation energy in intact cells of the thermophilic green photosynthetic bacterium Chloroflexus aurantiacus was studied both at low temperature and under more physiological conditions. Analysis of excitation spectra measured at 4K indicates that the minor fraction of bacteriochlorophyll a present in the chlorosome functions as an intermediate in energy transfer between the main light-harvesting pigment BChl c and the membrane-bound B808-866 antenna complex. This supports the hypothesis that BChl a is associated with the base plate which connects the chlorosome with the membrane. The overall efficiency for energy transfer from the chlorosome to the membrane is only 15% at 4K. High efficiencies of close to 100% are observed above 40°C near the temperature where the cultures are grown. Cooling to 20°C resulted in a sudden drop of the transfer efficiency which appeared to originate in the chlorosome. This decrease may be related to a lipid phase transition. Further cooling mainly affected the efficiency of transfer between the chlorosome and the membrane. This effect can only partially be explained by a decreased Förster overlap between the chlorosomal BChl a and BChl a 808 associated with the membrane-bound antenna system. The temperature dependence of the fluorescence yield of BChl a 866 also appeared to be affected by lipid phase transitions, suggesting that this fluorescence can be used as a native probe of the physical state of the membrane. 相似文献
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