The permeability of the cell-to-cell membrane channel and its regulation in mammalian cell junctions

Summary Mammalian cell-to-cell channels show polar permselective properties discriminating against negatively charged 14 ?-wide molecules and are more restrictive than the channels of insect cell junctions. The channel permeability is modulated by conditions affecting the concentration of intracellular ionic Ca: elevation of the external Ca load (B cells), treatment of cell cultures with Ca-transporting ionophore (in the presence of external Ca, but not in its absence), treatment with a combination of cyanide and iodoacetate, or with high levels of carbon dioxide, all cause depression of channel permeability. Treatment of cell cultures with cyclic AMP or its more permeable derivative, dibutyryl cyclic AMP, produces increase in permeability. A similar channel up regulation is observed upon elevation of the endogenous level of cyclic AMP by serum deprivation or lowering of cell density. Presented in the symposium on Molecular and Morphological Aspects of Cell-Cell Communication at the 31st Annual Meeting of the Tissue Culture Association, St. Louis, Missouri, June 1–5, 1980. This symposium was supported in part by Contract 263-MD-025754 from the National Cancer Institute and the Fogarty International Center. This work was supported by grant number 5 R01 CA14464, awarded by the National Cancer Institute, DHEW.     

The permeability of the cell-to-cell membrane channel and its regulation in mammalian cell junctions

Mammalian cell-to-cell channels show polar permselective properties discriminating against negatively charged 14 A-wide molecules and are more restrictive than the channels of insect cell junctions. The channel permeability is modulated by conditions affecting the concentration of intracellular ionic Ca: elevation of the external Ca load (B cells), treatment of cell cultures with Ca-transporting ionophore (in the presence of external Ca, but not in its absence), treatment with a combination of cyanide and iodoacetate, or with high levels of carbon dioxide, all cause depression of channel permeability. Treatment of cell cultures with cyclic AMP or its more permeable derivative, dibutyryl cyclic AMP, produces increase in permeability. A similar channel up regulation is observed upon elevation of the endogenous level of cyclic AMP by serum deprivation or lowering of cell density.     

Induced changes in permeability of plant cell membranes to water

The half-time for THO equilibration was three times longer for a living carrot (Daucus carota L.) cylinder than for a dead one. Furthermore, the energy of activation of THO flux was more than twice as high for the living cylinder. Passage through living membranes thus constitutes a rate-limiting step for THO flux in carrot tissue.     

The possible influence of osmotic poration on cell membrane water permeability

It has been hypothesized that pores in the plasma membrane form under conditions of rapid water efflux, allowing extracellular ice to grow into the cytoplasm under conditions of rapid freezing. When cells with intracellular ice are thawed slowly, the transmembrane ice crystal expands through recrystallization causing the cell to lyse. One of the implications of this hypothesis is that osmotic pores will provide an alternative route for water movement under conditions of osmotically induced flow. We show that the plasma membrane water permeability of a fibroblast cell changes as a function of the osmotic pressure gradient that is used to drive water movement. It is further shown that cell volume is more important than the magnitude of water flux in causing this departure from a uniform water permeability. We suggest that these data provide evidence of a transient route for water movement across cell membranes.     

NMR study of the selective inhibition of water permeability of rat erythrocyte membrane

The inhibition of water diffusion across the rat erythrocyte membrane was studied by NMR using two basically different types of inhibitory agents: PCMB andin vivo irradiation. The contribution of lipid and protein to water permeability revealed the inhibitory effect of each pathway. Internal contamination with tritium (25–115 mGy) reduces water permeability due to protein modifications; for doses higher than 100 mGy the lipid mediated mechanism seems also to be impaired. The same procedure enables one to assess the extent to which the higher water permeability of rat, compared to human, erythrocyte is due to one of the two pathways.     

Water permeability in human erythrocytes: identification of membrane proteins involved in water transport

The water permeability of human erythrocytes has been monitored by nuclear magnetic resonance (NMR) before and after treatment of the cells with various sulfhydryl reagents. Preincubation of the cells with N-ethylmaleimide (NEM), a non-inhibitory sulfhydryl reagent, results in a faster and more sensitive inhibition of water exchange by mercurials. The inhibition of water exchange by p-chloromercuribenzene sulfonate (PCMBS) was maximal at a binding of approximately 10 nmol PCMBS per mg protein when non-specific sulfhydryl groups are blocked by NEM. Inhibition by PCMBS has been correlated with the binding of 203Hg to erythrocyte membrane proteins. A significant binding of label to band 3 and the polypeptides in band 4.5 occurs, with approximately 1 mol of mercurial bound per mol of protein. Inhibition of water transport by sulfhydryl reagents does not induce major morphological changes in the cells as assessed by freeze-fracture and scanning electron microscopy.     

Ca2+ transport and permeability in inside-out red cell membrane vesicles after freezing

To evaluate the effects of freezing and thawing on Ca2+ transport and permeability, inside-out red cell membrane vesicles (IORCMV) are examined. Exposure to the cryoprotectant Me2SO as well as different cooling regimes on unprotected and cryoprotected vesicles do not affect the membrane Ca2+ transport. However, freezing and thawing increase the membrane permeability to sucrose.     

Probing water compartments and membrane permeability in plant cells by 1H NMR relaxation measurements

¹H NMR relaxation times (T₁ and T₂) in parenchyma tissue of apple can identify three populations of water with different relaxation characteristics. By following the uptake of Mn²⁺ ions in the tissue it is shown that the observed relaxation times originate from particular water compartments: the vacuole, the cytoplasm, and the cell wall/extracellular space.

Proton exchange between these compartments is controlled by the plasmalemma and tonoplast membranes. During the Mn²⁺ penetration experiment, conditions occur that cause the relaxation times of protons of cytoplasmic water to be much shorter than their residence time in the cytoplasm. Then the tonoplast permeability coefficient P_d for water can be calculated from the vacuolar T₁ and T₂ values to be 2.44 10^-5 m·s^-1.

     

Rickettsial cell water and membrane permeability determined by a micro space technique.

A micro space technique for determining membrane permeability in Rickettsia prowazeki is described and justified. The cell water, cell wall plus periplasmic volume, and glutamate, ethylene glycol, and adenosine diphosphate permeabilities were determined by this method. The effect of nonionic detergents on rickettsial permeability was examined: Triton X-100 destroyed the permeability barrier, whereas Lubrol-WX left it intact.     

Induction of water deficit tolerance in wheat due to exogenous application of plant growth regulators: membrane stability,water relations and photosynthesis

Our experiment was carried out in order to explore effects of plant growth regulators (PGR; thidiazuron, paclobutrazol, and ascorbic acid) on physiological traits of wheat genotypes under water surplus and deficit conditions. Study revealed that relative water content, membrane stability index, chlorophyll content, photosynthetic rate (P_N), and maximal quantum yield of PSII improved with PGRs application across the genotypes both under irrigation and water stress. The response of HD 2733 genotype was more positive toward PGRs treatment as compared to other genotypes under water stress. Higher P_N and chlorophyll contents were observed in HD 2987 followed by C 306 genotype under water-stress conditions. Moreover, Rubisco small subunit (SSU) expression was lower in wheat genotypes under water stress as compared to irrigated conditions. Application of PGRs led to upregulation of SSU under water stress, while no significant change was found in Rubisco level and activity under irrigated condition in dependence on PGRs treatments. Yield-related traits showed also significant reduction under water-stress conditions, while application of PGRs enhanced the yield and its components. Results indicated that the PGRs exhibited a positive interaction and synergetic effect on water stressed wheat plants in terms of photosynthetic machinery and yield.     

Short-term control of maize cell and root water permeability through plasma membrane aquaporin isoforms

Although it is widely accepted that aquaporins are involved in the regulation of root water uptake, the role of specific isoforms in this process is poorly understood. The mRNA expression and protein level of specific plasma membrane intrinsic proteins (PIPs) were analysed in Zea mays in relation to cell and root hydraulic conductivity. Plants were analysed during the day/night period, under different growth conditions (aeroponics/hydroponics) and in response to short-term osmotic stress applied through polyethylene glycol (PEG). Higher protein levels of ZmPIP1;2, ZmPIP2;1/2;2, ZmPIP2;5 and ZmPIP2;6 during the day coincided with a higher water permeability of root cortex cells during the day compared with night period. Similarly, plants which were grown under aeroponic conditions and which developed a hypodermis ('exodermis') with Casparian bands, effectively forcing more water along a membranous uptake path across roots, showed increased levels of ZmPIP2;5 and ZmPIP1;2 in the rhizodermis and exodermis. When PEG was added to the root medium (2-8 h), expression of PIPs and cell water permeability in roots increased. These data support a role of specific PIP isoforms, in particular ZmPIP1;2 and ZmPIP2;5, in regulating root water uptake and cortex cell hydraulic conductivity in maize.     

Investigation of membrane permeability of carp spermatozoa for water molecules

The fundamentals of a photometry method for determination of membrane permeability of some fish spermatozoa for water molecules are presented. Osmotic tolerance of carp spermatozoa membranes was studied using EPR-spectroscopy and photometric analysis methods. It was shown that carp spermatozoa look like the ideal osmometers in their reaction on media of different osmolarity. The value of membrane permeability of carp spermatozoa for water molecules was determined. Data obtained can be used in cryobiology for creating cryoprotective media and regimes of fish sperm cryopreservation.     

Mitochondrial calcium transport and permeability transition as rational targets for plant protection

The mitochondrial permeability transition (MPT) is a death-inducing mechanism that collapses electrochemical gradients across inner mitochondrial membranes. Several studies in model plants have detailed potential MPT-dependent cell death upon abiotic stress in response to heat shock, ultraviolet radiation, heavy metal toxicity and waterlogging. However, the molecular specifics of the MPT and its possible role on plant cell death remain controversial. This review addresses previous and recent developments on the role(s) of the MPT in plants. Considering these advances, MPT targeting can constitute a plausible strategy to ameliorate cell death in plants upon abiotic stress.     

Structural determinants of water permeability through the lipid membrane

Despite intense study over many years, the mechanisms by which water and small nonelectrolytes cross lipid bilayers remain unclear. While prior studies of permeability through membranes have focused on solute characteristics, such as size, polarity, and partition coefficient in hydrophobic solvent, we focus here on water permeability in seven single component bilayers composed of different lipids, five with phosphatidylcholine headgroups and different chain lengths and unsaturation, one with a phosphatidylserine headgroup, and one with a phosphatidylethanolamine headgroup. We find that water permeability correlates most strongly with the area/lipid and is poorly correlated with bilayer thickness and other previously determined structural and mechanical properties of these single component bilayers. These results suggest a new model for permeability that is developed in the accompanying theoretical paper in which the area occupied by the lipid is the major determinant and the hydrocarbon thickness is a secondary determinant. Cholesterol was also incorporated into DOPC bilayers and X-ray diffuse scattering was used to determine quantitative structure with the result that the area occupied by DOPC in the membrane decreases while bilayer thickness increases in a correlated way because lipid volume does not change. The water permeability decreases with added cholesterol and it correlates in a different way from pure lipids with area per lipid, bilayer thickness, and also with area compressibility.