The breeding biology of White-tailed Tropicbirds Phaethon lepturus at Cousin Island, Seychelles

During 1981–1983 the population of White-tailed Tropicbirds Phaethon lepturus at Cousin Island, Seychelles, bred aseasonally and asynchronously. The mean interval between consecutive successful breeding attempts was 273 days. Most individuals nested in the same site, and with the same partner, in successive attempts, but 7-5% of nest sites were used by more than one pair; 21 % of pairs which lost eggs, and 13% of those which lost chicks re-nested. Incubation lasted 40–41 days, with a mean incubation shift length of 6-6 days. Fledging periods varied between 67 and 89 days (mean 76-5 days). Mean fledging weight was not significantly greater than the average weight of breeding adults.
Fledging success was 36%. Many of the 39% of eggs which failed to hatch probably did so as a result of exposure to the sun's heat, following temporary desertion by adults which were themselves heat-stressed during incubation. Of the 41 % of chicks which died, most died young; many were probably killed by crabs Ocypode spp. Not more than 10% of all nest failures were due to nest site competition. A few chicks probably starved to death.
At the time of the study, both food and nesting sites were evidently in excess. A considerable increase in the size of the population will be possible before density-dependent control mechanisms begin to operate.     

Poxvirus infection of the Manx shearwater (Puffinus puffinus)

During studies on the etiology of puffinosis, a disease of the Manx shearwater, 1 to 4% of full-grown birds were found to have dry, non-pigmented lesions on the webs of the feet. Poxvirus infection was detected in six of seven full-grown birds with such lesions. The lesions contained large encapsulated inclusions which were packed with mature and immature poxvirus particles. Poxvirus infection was not apparent in shearwater fledglings during puffinosis epizootics, and its spatial distribution was not related to that of puffinosis. The results indicate that poxvirus infection produces a mild, self-limiting disease in shearwaters and is not the cause of puffinosis.     

Poxvirus infection in a colony of common marmosets (Callithrix jacchus)

An epizootic poxvirus infection occurred in a colony of 80 common marmosets (Callithrix jacchus) recently introduced to a laboratory facility. Over an 18-week period, 29 of the monkeys exhibited skin lesions that persisted for 4-6 weeks. Although eight marmosets died during the outbreak, their deaths were not attributed directly to the poxvirus infection. The skin lesions developed over the entire body surface including the soles and palms. Initially characterized as erythematous papules, they quickly changed to elevated coalescing lesions with extensive scab formation. Histopathologically, the lesions revealed moderate to marked acanthosis, and they progressed to full-thickness epidermal necrosis and ulceration. Intracytoplasmic inclusion bodies were observed occasionally within degenerate keratinocytes. These inclusions most probably constituted the intracytoplasmic aggregates of viral particles observed ultrastructurally and confirmed as members of the poxvirus group by negative staining of direct skin scrapings.     

Poxvirus Orthologous Clusters (POCs)

Poxvirus Orthologous Clusters (POCs) is a JAVA client-server application which accesses an updated database containing all complete poxvirus genomes; it automatically groups orthologous genes into families based on BLASTP scores for assessment by a human database curator. POCs has a user-friendly interface permitting complex SQL queries to retrieve interesting groups of DNA and protein sequences as well as gene families for subsequent interrogation by a variety of integrated tools: BLASTP, BLASTX, TBLASTN, Jalview (multiple alignment), Dotlet (Dotplot), Laj (local alignment), and NAP (nucleotide to amino acid alignment).     

Geographical variation in the White-tailed Tropicbird Phaethon lepturus, with the description of a new subspecies endemic to Europa Island, southern Mozambique Channel

Geographical variation in measurements and colouration among populations and subspecies of White-tailed Tropicbirds Phaethon lepturus , including birds from Europa Island (southern Mozambique Channel] was examined worldwide. Two groups were distinguished: the 'large subspecies' (lepturus and fulvus from the Indian Ocean, catesbyi from the western Atlantic Ocean) and the 'small subspecies' (ascensionis from the central and eastern Atlantic Ocean, dorotheae from the Pacific Ocean, and the birds from Europa Island). No clinal variation was found in the Indian Ocean, the birds from Europa Island being the only 'small' ones. This population also had a high frequency of golden morphs, a feature that does not exist elsewhere in the western Indian Ocean. These results indicate that Europa's population is isolated from all nearby colonies in the Indian Ocean, and does not belong to any of the two previously known subspecies of the area. It also differs from the birds of the two small subspecies by the frequency of the colour morphs and the distribution. Consequently, we propose to treat this population as a previously undescribed subspecies, endemic to Europa Island, for which we propose the name Phaethon lepturus europae. Geographical isolation of Europa Island and oceanic conditions in the Mozambique Channel are discussed to explain the isolation of this population.     

Poxvirus infection in the white-backed magpie (Gymnorhina hypoleuca) and pox-like conditions in other birds in Australia.

Lesions, grossly and histologically typical of pox infection, occurred in a white-backed magpie from Melbourne, Australia. Electron microscopic examination revealed typical poxvirus particles in lesion material. The disease was experimentally transmitted to other magpies, but chickens, turkeys, pigeons, and canaries were refractory to experimental infection with magpie poxvirus. The epidemiology of magpie pox and the probable occurrence of pox-like disease in other native Australian birds are discussed.     

Persistent Ehrlichia chaffeensis infection in white-tailed deer

Four white-tailed deer (Odocoileus virginianus) were inoculated intravenously with a deer-origin isolate (15B-WTD-GA) of Ehrlichia chaffeensis. The course of infection was monitored using indirect fluorescent antibody (IFA), polymerase chain reaction (PCR), and culture over a 9 m period. All deer became rickettsemic within 24 days post inoculation (DPI), and all developed antibody titers >1:64 to E. chaffeensis by 17 DPI. Titers in all deer fell below 1:64 during 87 to 143 DPI. One deer exhibited a second period of seropositivity (peak titer of 1:256) from 207 to 271 DPI but was culture and PCR negative during this period. Rickettsemia was confirmed by reisolation of E. chaffeensis as late as 73 to 108 DPI in three deer. Positive PCR results were obtained from femur bone marrow of one deer and from rumenal lymph node of another (leer at 278 DPI. None of the deer developed clinical signs, hematologic abnormalities, or gross or microscopic lesions attributable to E. chaffeensis. Two uninoculated control deer were negative on all tests through 90 DPI at which time they were removed from the study. Herein we confirm that white-tailed deer become persistently infected with E. chaffeensis, have initial rickettsemias of several weeks duration and may experience recrudescence of rickettsemia, which reaffirm the importance of deer in the epidemiology of E. chaffeensis.     

Experimental infection of white-tailed deer (Odocoileus virginianus) with Mycobacterium avium subsp. paratuberculosis

Mycobacterium avium subsp. paratuberculosis (Map) is the causative agent of paratuberculosis or Johne's disease, a chronic enteric disease of domestic ruminants as well as some nondomestic ruminants. Paratuberculosis is characterized by a protracted subclinical phase followed by clinical signs such as diarrhea, weight loss, and hypoproteinemia. Fecal shedding of Map is characteristic of both the subclinical and clinical phases, and it is important in disease transmission. Lesions of paratuberculosis are characterized by chronic granulomatous enteritis and mesenteric lymphadenitis. Animal models of paratuberculosis that simulate all aspects of the disease are rare. Oral inoculation of 9-day-old white-tailed deer (Odocoileus virginianus) on 3 June 2002 with 1.87 x 10(10) colony-forming units of Map strain K10 resulted in clinical disease (soft to diarrheic feces) as early as 146 days after inoculation; lesions consistent with paratuberculosis were observed in animals at the termination of the study. Intermittent fecal shedding of Map was seen between 28 and 595 days (4 March 2004) after inoculation. These findings suggest that experimental oral inoculation of white-tailed deer fawns may mimic all aspects of subclinical and clinical paratuberculosis.     

Nitric oxide production as an indication of Mycobacterium bovis infection in white-tailed deer (Odocoileus virginianus)

White-tailed deer (Odocoileus virginianus) are reservoirs for Mycobacterium bovis in northeast Michigan, USA. Production of nitric oxide (NO) by activated macrophages is a potent mechanism of mycobacterial killing. The capacity of macrophages to produce NO, however, varies among mammalian species. The objective of this study was to determine if mononuclear cells from white-tailed deer produce nitrite as an indication of NO production and, if so, is NO produced in response to stimulation with M. bovis antigens. Supernatants were harvested from adherent peripheral blood mononuclear cell (PBMC) cultures that had been stimulated with either Mannheimia haemolytica lipopolysaccharide (LPS) or media alone (i.e., no stimulation). Nitrite levels within M. haemolytica LPS-stimulated culture supernatants exceeded (P < 0.05) those detected within supernatants from non-stimulated cultures as well as those detected within supernatants from cultures receiving an inhibitor of NO synthase in addition to M. haemolytica LPS. In response to stimulation with M. bovis antigens, nitrite production by PBMC from M. bovis-infected deer exceeded (P < 0.05) the production by PBMC from non-infected deer. The response of PBMC from infected deer to M. bovis antigens exceeded (P < 0.05) the response of parallel cultures from the same deer receiving no stimulation. The response of PBMC from M. bovis-infected deer to M. avium antigens did not differ from that of PBMC from M. bovis-infected deer to no stimulation or from that of PBMC from non-infected deer to M. avium antigens. These findings indicate that adherent PBMC from white-tailed deer are capable of NO production and that mononuclear cells isolated from M. bovis-infected white-tailed deer produce NO in an antigen-specific recall response.     

Experimental infection of white-tailed deer (Odocoileus virginianus) with Ehrlichia chaffeensis by different inoculation routes

The infection dynamics of the tick-transmitted organism Ehrlichia chaffeensis were investigated in white-tailed deer (Odocoileus virginianus) using different routes of inoculation. Six deer were each inoculated with 5.4 x 10(6) DH82 cells infected with E. chaffeensis (Arkansas strain) by three different routes: intravenous (n = 2), subcutaneous (n = 2), and intradermal (n = 2). Two control deer were inoculated with uninfected cells. Infections were monitored for 54 days and were continued in one deer from each E. chaffeensis inoculated group for an additional 31 days. All deer inoculated with E. chaffeensis seroconverted (> or = 1: 64) and became 16S rDNA polymerase chain reaction and/or cell culture positive by post-inoculation day 15. There was no apparent (difference in susceptibility to infection between deer inoculated by different routes for the first 50 days based on detection of E. chaffeensis infection by PCR assay of blood or culture isolation. These results demonstrate infection of (deer by intradermal and subcutaneous routes for the first time.     

Congenital osteopetrosis in white-tailed deer (Odocoileus virginianus)

Inferior brachygnathia in neonatal fawns occurred sporadically over a 10 yr period in a captive herd of white-tailed deer (Odocoileus virginianus) in southern Ontario. Two fawns submitted for necropsy had marked inferior brachygnathia, protruding tongues, and fractured long bones. Radiographs of the limbs revealed longitudinal striations of relatively translucent immature woven bone that caused loss of distinction between medullary cavities and cortices. Microscopically, there was failure of remodelling of the primary spongiosa and filling of the medulla by cone-shaped chondro-osseous cores. The findings supported a diagnosis of osteopetrosis, usually a hereditary disease characterized by absence of marrow cavities as a result of defective bone remodelling. Osteopetrosis has not been reported previously in deer.     

Hyperkalemia in free-ranging white-tailed deer (Odocoileus virginianus)

Sixty adult and yearling female white-tailed deer (Odocoileus virginianus) were collected in July 2008 (n=30) and March 2009 (n=30) from eastern North Carolina as part of a population health assessment. During July 2008, standard serum analyses revealed hyperkalemia in all deer sampled. In March, the effect of processing time as a possible source of the hyperkalemia was investigated. For a subset of deer (n=10), blood tubes were centrifuged and processed at four time points (0, 30, 60, and 120 min) postcollection. Delayed centrifugation and plasma separation did not affect potassium (K(+)) concentration over time, indicating that a shift in intracellular K(+) did not occur and the hyperkalemia was not due to improper sample handling. Potassium levels were negatively correlated with age and varied across collection periods. Also, K(+) levels were positively correlated with glucose and not correlated with creatine kinase (CK). No single variable indicated a strong enough relationship to explain the hyperkalemia in the study.     

Theperistome of Ischyrodon lepturus (Tayl.) Schelpe

Abstract

Examination of the peristome of lschyrodon lepturus (Tayl.) Sehelpe, found in fruit for the first time, shows it to be congeneric with I. seriolus C. Müll. and the genus to belong to Fabroniaceae rather than Myriniaceae.     

Experimental infection of white-tailed deer with rangiferine brucellosis

Experimental infections of white-tailed deer (Odocoileus virginianus) with Brucella suis biovar 4 were evaluated over a period of 6 wk. Five adult male hand-raised white-tailed deer were inoculated with 1 x 10(7) colony forming units of B. suis biovar 4 in the conjunctiva and serologically evaluated over 6 wk by the card test (CARD), rivanol test (RIV), serum agglutination test (SAT), complement fixation test (CFT), particle concentration fluorescence immunoassay (PCFIA), and competitive enzyme linked immunosorbent assay (cELISA), as routinely used for bovine samples. Six weeks postinoculation the animals were euthanized and cultured for B. suis biovar 4. One deer became serologically positive 4 wk postinoculation on CFT, CARD, PCFIA, and cELISA. At 6 wk postinoculation, CFT was positive in four infected deer, CARD was positive in three deer; RIV, SAT, and PCFIA was positive in two deer; and cELISA was positive in one deer. Only the CFT was 100% sensitive. At necropsy B. suis biovar 4 was isolated from four of five deer, and representative colonies were biologically similar to the challenge organism.     

Theileriosis in a white-tailed deer (Odocoileus virginianus) fawn

A white-tailed deer (Odocoileus virginianus) fawn was collected in Missouri (USA) and submitted for diagnostic evaluation. Necropsy and histologic examination revealed severe Amblyomma americanum infestation, pronounced icterus, and marked hemosiderin deposition in the liver and spleen. Whole blood evaluation revealed a normocytic normochromic anemia and a piroplasm parasitemia of approximately 70%. The piroplasm was identified as Theileria cervi by polymerase chain reaction and sequencing of the V4 variable region of the 18S rRNA gene from a paraffin-embedded section of lung. Although T. cervi parasitemias have been commonly reported in healthy white-tailed deer, the severe parasitemia in this fawn may have contributed to overt clinical disease, perhaps as part of a combined malnutrition and parasitemia syndrome.     

Maxillary lymphosarcoma in a white-tailed deer (Odocoileus virginianus)

In 1996, lymphosarcoma was observed in a captive adult female white-tailed deer (Odocoileus virginianus) from northeastern Kansas (USA). A subcutaneous mass on the deer's left cheek was surgically removed and lymphosarcoma was diagnosed. The mass recurred within 3 wk. A second surgical removal was attempted but the tumor had grown much larger, had become intimately involved with the buccal mucosa, and was beginning to interfere with mastication. For these reasons, the deer was euthanized. At postmortem examination the only abnormal findings were the primary tumor and enlarged ipsilateral parotid and mandibular lymph nodes. Histologically these tissues demonstrated changes characteristic of lymphosarcoma but no other organs had evidence of neoplastic disease. A diagnosis of focal lymphosarcoma with local metastasis was made. The organ distribution of lymphosarcoma in this deer differs from previously described cases of lymphosarcoma in cervids.     

Failure to detect infection in fallow deer (Cervus dama) exposed to Theileria cervi from white-tailed deer

A frozen stabilate was produced from Theileria cervi sporozoites in salivary glands of adult Amblyomma americanum. The stabilate was inoculated into three fallow deer (Cervus dama) and two white-tailed deer (Odocoileus virginianus). Following inoculation, the white-tailed deer developed parasitemias as determined by blood smear examination at 11 and 13 days postexposure. Repeat examination of blood from the three fallow deer for 30 days postexposure failed to reveal observable piro-plasms. These findings indicate that fallow deer are not as susceptible to the Theileria cervi found in white-tailed deer from North America. Thus, there are some questions regarding the taxonomic position of this organism.     

Staphylococcal arthritis in a white-tailed deer (Odocoileus virginianus)

A five and one-half year old deer (Odocoileus virginianus) developed an ankylosing periarticular hypertrophic arthritis due to a coagulase positive staphylococcus infection. Complete encasement of the tibio-tarsal (hock) joints with a hypertrophic, and porous osseous mass had occurred with complete loss of articular cartilage. The pathologic alterations of the tissues are compared to those occurring in swine due to Erysipelothrix insidiosa infection which produces a rheumatoid-like arthritis.