Recommendations for sampling littoral diatoms in lakes for ecological status assessments

This review summarises the existing literature and outlines the theoretical basis for using standard methods for sampling diatoms from rivers to sample littoral diatoms and other phytobenthos from standing waters. The European Union's Water Framework Directive has created a statutory obligation for EU Member States to monitor macrophytes and phytobenthos in lakes. Although there has been a considerable amount of work using phytobenthos (especially diatoms) to monitor river water quality in Europe, there are fewer studies on the use of phytobenthos for monitoring in lakes. European standard methods for sampling diatoms from rivers should be suitable, with only minor modifications, for sampling littoral diatoms from lakes and other standing water bodies. These recommendations should be applicable to all temperate regions.     

The use of diatoms to assess past and present water quality

Abstract Diatoms possess a number of attributes which contribute to their suitability as biological indicators. They are highly sensitive to water chemistry changes, abundant in aquatic environments, largely cosmopolitan in distribution, less habitat dependent than macroinverte-brates and have a well-studied taxonomy and ecology. Furthermore, the preservation of diatom valves in lake sediments means that they can provide otherwise unavailable baseline data which can be used to assess and contextualize human impacts on aquatic ecosystems. The value of diatoms as bioindicators in contemporary and palaeolimnological studies has been well established overseas. Despite this, they have been under-utilized in Australia. This paper outlines some of the applications and potential for the use of diatoms as biological indicators in Australia.     

Are diatoms good integrators of temporal variability in stream water quality?

1. Although diatoms have been used for many decades for river monitoring around the world, studies showing evidence that diatoms integrate temporal variability in water chemistry are scarce. 2. The purpose of this study was to evaluate the response of the Eastern Canadian Diatom Index (IDEC: Indice Diatomées de l’Est du Canada) with respect to temporal water chemistry variability using three different spatio‐temporal data sets. 3. Along a large phosphorus gradient, the IDEC was highly correlated with averaged water chemistry data. Along within‐stream phosphorus gradients, the IDEC integrated phosphorus over various periods of time, depending on the trophic status of the site studied (Boyer, Nicolet or Ste. Anne river) and variability in nutrient concentration. 4. In the Ste. Anne River, where nutrient concentrations were low and generally stable, an input of phosphorus induced a rapid change in diatom community structure and IDEC value within the following week. In the mesotrophic Nicolet River, the observed integration period was approximately 2 weeks. Diatom communities in the eutrophic Boyer River appeared to be adapted to frequent and significant fluctuations in nutrient concentrations. In this system, the IDEC therefore showed a slower response to short term fluctuations and integrated nutrient concentrations over a period of 5 weeks. 5. Our results suggest that the integration period varies as a function of trophic status and nutrient concentration variability in the streams. Oligotrophic streams are more sensitive to nutrient variations and their diatom communities are directly altered by nutrient increase, while diatom communities of eutrophic rivers are less sensitive to nutrient fluctuations and major variations take a longer time to be integrated into index values. 6. The longer integration period in the eutrophic environment may be attributed to the complexity of the diatom community. The results from this study showed that the diversity and evenness of the communities increased with trophic status.     

Ecology of the Major Periphytic Diatom Communities from the Mesta River,Bulgaria

Epilithon, epiphyton, epipelon, epipsammon and plocon diatom samples and water chemistry samples were collected bimonthly from 11 stations along the Mesta River, Bulgaria between December 1989 and April 1991. Principal component analysis (PCA), correlation, and dominance analysis were employed for describing the seasonal dynamics of diatom assemblages and estimating the correlation between diatom distribution and the physico-chemical parameters. All periphytic communities were compared by PCA, MANOVA and Fisher's LSD multiple comparison test. Ionic strength and eutrophication were the major abiotic factors affecting diatom distribution in the epilithon, epiphyton, epipelon and epipsammon. Current velocity had an important influence on the diatom assemblages from the epilithon and epiphyton. Epilithon showed the lowest correlation with most of the environmental factors and had significantly lower species diversity than epiphyton, epipelon and epipsammon. Multivariate analysis of the diatom data suggested that in all benthic habitats of the Mesta River seasonal replacement of diatom assemblages was controlled by the water chemistry rather than substratum, current velocity or discharge. The oligotrophic, mesotrophic and eutrophic diatom communities in the Mesta River comprised early-, mid- and late-successional colonizers respectively. Multiple comparisons of all periphytic communities showed that habitat specificity was positively correlated with current velocity.     

Using diatoms to assess the biological condition of large rivers in Idaho (U.S.A.)

SUMMARY 1. Diatoms were collected from 49 sites located on 23 rivers and identified to species. We defined 26 attributes of the diatom assemblage on the basis of tolerance and intolerance, autecological guild, community structure, morphological guild and individual condition. 2. We grouped sites into three geographical regions on the basis of altitude, land cover and use. Within each region, we tested for an association between diatom attributes and human disturbance. 3. We selected nine attributes for inclusion in a multimetric index, the river diatom index (RDI): percentage of valves belonging to species sensitive to disturbance, percentage of valves belonging to species very tolerant of disturbance, eutrophic species richness, percentage of valves belonging to nitrogen heterotrophic species, percentage of valves belonging to polysaprobic species, alkaliphilic species richness, percentage of valves belonging to species that require high oxygen, percentage of valves belonging to very motile genera and percentage of deformed valves. 4. The RDI was significantly correlated with measures of human disturbance made at the site (conductivity, percentage of fine sediments and number of human activities) and at the catchment level (percentage of urbanisation and agriculture in the upstream catchment). 5. The percentage of the total variance of RDI related to transect location was very small (1%) compared with among‐site differences (73%) and time of sampling (26%). The RDI could reliably detect approximately three categories of biological condition based on annual monitoring and potentially more if sampling were restricted to the same month each year.     

The effects of dissolved heavy metals on attached diatoms in the Uintah Basin of Utah,U.S.A.

The relationships of diatom species to dissolved heavy metals in the streams of the Uintah Basin of Utah were studied through four seasons of 1977–1978. Niche center gradient analysis, cluster analysis and correlation analysis were performed.Achnanthes minutissima, Cyclotella meneghiniana, Cymbella minuta, Gomphonema parvulum, Navicula secreta var.apiculata, Nitzschia frustulum, Nitzschia frustulum var.perminuta, Nitzschia frustulum var.perpusilla, Nitzschia palea, andSynedra ulna appear to be indicator species of high or low heavy metal concentrations. Several other species also showed meaningful relationships to high or low heavy metal concentrations.Dept. of Physics and Astronomy, Brigham Young UniversityDept. of Physics and Astronomy, Brigham Young University     

The effects of selected physical and chemical factors on attached diatoms in the Uintah Basin of Utah,U.S.A.

The relationships of diatom species to selected physical and chemical parameters in the streams of the Uintah Basin of Utah were studied through four seasons of 1977–1978. Niche center gradient analysis, cluster analysis and correlation analysis were performed.Achnanthes minutissima, Cyclotella meneghiniana, Cymbella minuta var.latens, Fragilaria capucina, andNavicula secreta var.apiculata appear to be indicator species of high or low levels of certain water quality parameters. Several other species also showed meaningful relationships to selected physical and chemical parameters.     

Comparative monitoring of diatoms, macroinvertebrates and macrophytes in the Woluwe River (Brussels, Belgium)

The river Woluwe in Brussels and Flanders (Belgium) is a small tributary of 15 km length that drains an area of 9400 ha in the Schelde river basin. The headwaters of the Woluwe are highly fragmented by diverse pond systems and are vaulted in the Brussels agglomeration. Hyporheic zones locally influence the water quality. The downstream stretch of the river receives sewage waters from households and industry. As the river Woluwe within a short distance represents a typical gradient from groundwater-fed sources in the forest towards severely polluted water, a comparative monitoring using diatoms, macroinvertebrates and macrophytes was done. The saprobic index based on diatoms, the Belgian Biotic Index (BBI) for macroinvertebrates and a macrophyte index based on the N-values of Ellenberg were used in this comparison and for estimating the correlation with the bimonthly measured chemical variables in 16 sampling stations. The diatom saprobic index and the macrophyte index were strongly correlated. Both groups showed strong correlations with phosphate, ammonium and chemical oxygen demand. The Belgian Biotic Index showed lower correlations with the nutrient variables, but was slightly better correlated to chemical oxygen demand, chloride and dissolved oxygen. None of the indices showed a correlation with nitrate. Local substrate or light conditions could interfere with the indicator system, especially for the macrophytes and occasionally for the macroinvertebrates. It was concluded that at least in this particular river system, the indices based on the primary producers were more indicative for the trophic status, whereas the BBI showed a broader relationship to the general degree of pollution. Therefore, these three indices are considered as complementary for monitoring the biological quality and the ecological status of a river system.     

The Trophic Diatom Index: a new index for monitoring eutrophication in rivers

A index for monitoring the trophic status of rivers based on diatom composition (‚trophic diatom index’, TDI) has been developed, in response to the National Rivers Authority (England & Wales)'s needs under the terms of the Urban Wastewater Treatment Directive of the European Community. The index is based on a suite of 86 taxa selected both for their indicator value and ease of identification. When tested on a dataset from 70 sites free of significant organic pollution, this index was more highly correlated with aqueous P concentrations than previous diatom indices. However, where there was heavy organic pollution, it was difficult to separate the effects of eutrophication from other effects. For this reason, the value of TDI is supplemented by an indication of the proportion of the sample that is composed of taxa tolerant to organic pollution. The index was tested on the R. Browney, N-E. England, above and below a major sewage discharge. TDI values indicated that the effect of inorganic nutrients on the river downstream of the discharge was slight as the river was already nutrient-rich, but there was a large increase in the proportion of organic pollution-tolerant taxa. This indicates that the river was already so eutrophic upstream of the discharge that tertiary treatment to remove P would not be effective unless other aspects of the discharge were also improved.     

Effects of hydrologic and water quality drivers on periphyton dynamics in the southern Everglades

Everglades periphyton mats are tightly-coupled autotrophic (algae and cyanobacteria) and heterotrophic (eubacteria, fungi and microinvertebrates) microbial assemblages. We investigated the effect of water column total phosphorus and nitrogen concentrations, water depth and hydroperiod on periphyton of net production, respiration, nutrient content, and biomass. Our study sites were located along four transects that extended southward with freshwater sheetflow through sawgrass-dominated marsh. The water source for two of the transects were canal-driven and anchored at canal inputs. The two other transects were rain-driven (ombrotrophic) and began in sawgrass-dominated marsh. Periphyton dynamics were examined for upstream and downstream effects within and across the four transects. Although all study sites were characterized as short hydroperiod and phosphorus-limited oligotrophic, they represent gradients of hydrologic regime, water source and water quality of the southern Everglades. Average periphyton net production of 1.08 mg C AFDW⁻¹ h⁻¹ and periphyton whole system respiration of 0.38 mg C AFDW⁻¹ h⁻¹ rates were net autotrophic. Biomass was generally highest at ombrotrophic sites and sites downstream of canal inputs. Mean biomass over all our study sites was high, 1517.30 g AFDW m⁻². Periphyton was phosphorus-limited. Average periphyton total phosphorus content was 137.15 μg P g⁻¹ and average periphyton total N:P ratio was 192:1. Periphyton N:P was a sensitive indicator of water source. Even at extremely low mean water total phosphorus concentrations ( ≤ 0.21 μmol l⁻¹), we found canal source effects on periphyton dynamics at sites adjacent to canal inputs, but not downstream of inflows. These canal source effects were most pronounced at the onset of wet season with initial rewetting. Spatial and temporal variability in periphyton dynamics could not solely be ascribed to water quality, but was often associated with both hydrology and water source.     

A direct immunoassay for detecting diatoms in groundwater as an indicator of the direct influence of surface water

Groundwater under the direct influence of surface water (GWUDISW) is of concern in communities where growing public demand on groundwater resources has resulted in increased withdrawals and hydraulic stress near surface water bodies. Under these conditions, contaminants such as methyl-tert butyl ether (MTBE) and biological materials have been detected in domestic wells. Other contaminants and pathogens associated with surface water are not routinely tested for in groundwater-supplied systems. To address the need for methods to easily identify potentially vulnerable supplies, a direct immunoassay for the quantitative detection of diatoms in raw water samples was developed as a measure of surface water influence on groundwater. Cell wall preparations from Nitzschia palea Kützing, a freshwater diatom found throughout North America, were used to produce a polyclonal antibody that was applied in a direct enzyme-linked immunosorbent assay (ELISA) developed to detect the presence of N. palea cell wall components. The direct immunoassay allows detection at 500 cells L⁻¹, a level similar to diatom concentrations observed in samples of groundwater collected near the test site. This investigation was the first attempt to utilize an ELISA as an indicator of surface water influence on groundwater. Further research is needed to develop more specific diatom-based monoclonal antibodies, determine cross-reactivity, and optimize sample processing and ELISA procedures for development of a standardized method.     

Use of benthic diatom communities to evaluate water quality in rivers of southern Poland

Biological and chemical data were processed to estimate trophic stage and degree of pollution in several streams and rivers in southern Poland. The majority were eutrophic and some of them heavily polluted; only a few were oligo-mesotrophic. The differences in the water quality of the rivers were reflected by different types of diatom community and also by the values for some diatom indices, which were calculated using the latest version of the 'Omnidia' database software. Except for the Sládeček's index, all diatom indices correlated significantly with organic load (COD), oxygen concentration, conductivity and most of the measured ions. Some indices showed a significant negative correlation with trophic level (expressed by NH₄-N and PO₄-P). In general, IPS (Specific Pollution Sensitivity Index) and GDI (Generic Diatom Index) indices gave the best results. Among the investigated diatom communities, only a few taxa indicated oligo-mesotrophy and oligo-β-mesosaprobity. Most of the sites were characterised by a greater relative contribution of eutraphent and tolerant ones as well as α-mesosaprobic and polysaprobic diatoms. This study suggests that the structure of benthic diatom communities and diatom indices, especially GDI, can be applied for monitoring rivers in Poland. This revised version was published online in June 2006 with corrections to the Cover Date.     

The use of fish in ecological assessments

Abstract Fish provide powerful tools for assessing aquatic environments. Three attributes are especially significant: the sensitivity of fish to most forms of human disturbance, their usefulness at all levels of biological organization and the favourable benefit-to-cost ratio offish assessment programmes. Fish can be used as indicators over wide temporal and spatial ranges. Because they cover all trophic levels of consumer ecology, fish can effectively integrate the whole range of ecological processes in waterways. Fish have been used in many different roles for assessing river health and monitoring responses to remedial management. Three of these applications appear to have particular value for management of Australian rivers: (i) automated systems monitoring fish ventilation can provide sensitive, broad-spectrum and continuous sensing of water quality to protect receiving waters or water-supply intakes; (ii) programmes collecting routine data on commercial or recreational fisheries can be designed and analysed so as to isolate confounding effects due to fishery-specific factors and, hence, used to detect and monitor environmental change on large scales; (iii) the Index of Biotic Integrity (IBI) can be modified to suit Australian conditions and fish communities to meet the important need for a predictive model of aquatic environmental quality. The IBI is a quantitative biological tool with a strong ecological foundation that integrates attributes from several levels of ecosystem organization. Examples of the use of IBI elsewhere suggest its robustness, flexibility and sensitivity can cope effectively with the low diversity of the Australian fish fauna and the dominance of ecological generalists. A provisional structure is suggested for a test of the IBI in four riverine regions of New South Wales.     

Effects of water quality and season on diatom community structure in the Damour River,Lebanon

The diatom flora in the lower Damour River, a small coastal river in Lebanon, was studied during 1981. The study area included the Damour estuary and a tributary, the el-Hamam River. Achnanthes minutissima was the most abundant diatom in the flora. Other dominant taxa included Nitzschia dissipata and Navicula cryptocephala var. veneta in the spring and Anomoeoneis vitrea in the fall. The diatom community structure was characterized by 1 or 2 dominant taxa, a few common and many rare taxa, but the taxa in these categories varied according to season and locality. The freshwater flora had a group of 20 taxa which were widely distributed in the study area throughout the study period. These taxa, in addition to a few taxa of more restricted seasonal occurrence, were the major contributers to the flora and are characteristic of this river system. Certain aspects of the flora were resistant to change. Others were more sensitive to environmental conditions. The estuarine flora reflected brackish conditions beginning in June and extending through October.     

Colonization processes of diatoms on artificial substrates in the River Danube near Budapest (Hungary)

Periphyton was collected from sand-blasted slides exposed in the main channel of River Danube at Gö (1669 rkm). Samples were taken from 21 May to 23 November in 1984. Three to four days after placing the substrates into the river the first traces of an observable coating appeared. The formation of the algal coating in the Danube presented certain periodical features. From time to time, an essential decrease in the number of individuals could be observed, which generally coincided with changes in the struc- ture of the community. This could be traced well, by grouping the algae according to morphological categories and following the temporal changes affecting these categories. The early phase of the colo- nization process is dominated by relatively large araphid and biraphid diatoms. In the second phase small bi- and monoraphid species are dominant. Finally, the medium size mono- and biraphid algae are dominating.     

基于遥感的官厅水库水质监测研究

遥感监测具有监测面积广、速度快、成本低等优势,常用于大面积水质监测。以北京官厅水库为研究对象,通过野外和实验室测量数据建立水质参数遥感反演的生物光学模型,对夏季官厅水库的非色素颗粒物浓度、叶绿素a浓度和有色可溶性有机物(CDOM)浓度进行了反演。该模型研究的目的就是通过建立反演模型,利用卫星数据进行水质参数反演,从而得到大面积水体的水质分布图。采用CHRIS/Proba高光谱数据反演官厅水库的水体组分浓度,对库区水质反演要素的空间分布规律进行了分析。结果表明,所采用的遥感反演模型基本适用于官厅水库水质监测,反演出的叶绿素a、总悬浮物和CDOM的空间分布与实际测量值的空间分布基本吻合。     

Assessment of ecological status in U.K. rivers using diatoms

1. The European Union's Water Framework Directive requires all water bodies to achieve ‘good ecological status’ by 2015 and this paper describes a rationale for defining ‘good ecological status’ based on diatoms, a significant component of the biological quality element ‘macrophyte and phytobenthos’. 2. A database of benthic diatom samples collected over the past 20 years was assembled. New sampling, specifically for this project, was undertaken during 2004 to supplement these data. In total 1051 samples were included in the database with matching environmental data. 3. ‘Reference sites’, relatively unimpacted by human activity, were selected from this database by a series of screening steps and these sites were used to develop a site‐specific reference typology. 4. Environmental variables not related to the pressure gradient were used to predict the ‘expected’ Trophic Diatom Index (TDI) values at each site. Site‐specific TDI predictions were used to generate ecological quality ratios (EQRs) ranging from ≥1, where the diatom assemblage showed no impact, to (theoretically) 0, when the diatom assemblage was indicative of major anthropogenic activities. 5. The boundary between ‘high’ and ‘good’ status was defined as the 25th percentile of EQRs of all reference sites. The boundary between ‘good’ and ‘moderate’ status was set at the point at which nutrient‐sensitive and nutrient‐tolerant taxa were present in equal relative abundance. An ecological rationale for this threshold is outlined in the paper.     

Diatom Communities and Water Quality Assessment in Mountain Rivers of the Upper Segre Basin (La Cerdanya,Oriental Pyrenees)

Epilithic diatoms of mountain rivers from the upper Segre catchment (Oriental Pyrenees) were studied in 1998, during three different seasons: March, July and September. Four rivers, the river Segre and its three most important tributaries, Duran, Molina and Querol, were sampled in upstream and downstream stretches. The diatom communities were comparable in all upstream stretches of these mountain rivers draining siliceous substrates. Dominant taxa were Achnanthidium subatomus, Diatoma mesodon, Encyonema cf. minutum, E. silesiacum, Fragilaria arcus, F. capucina, Gomphonema calcifugum, G. pumilum, Meridion circulare and Nitzschia pura. Changes in water quality in the downstream stretches lead to the appearance of pollution tolerant taxa, such as Eolimna minima, Gomphoneis minuta, Navicula gregaria, and Nitzschia inconspicua. As a result, the values obtained with the diatom water quality indices (IPS Specific Polluosensitivity Index, CEE and IBD Biological Diatom Index) decreased. The diatom community composition and the derived water quality values did not change in the upstream stretches over the year. In contrast, significant changes were observed in the downstream stretches with best water␣quality in July, during high flows due to melting snow, and worst values in September, during low␣discharge. The diatom indices, especially the IPS, showed a good performance in these mountain rivers.     

Perspectives for the use of diatom assemblages in the water management policy of Overijssel (The Netherlands)

Diatoms have been sampled in several types of water bodies in the province of Overijssel for a period of five years. Samples from 333 sites were examined both in spring and autumn. Extensive analysis of physico-chemical variables was carried out.Data were processed by multivariate analysis for detecting correlations between species and environmental factors. Both ordination and clustering techniques were used. Statistical tests were performed to evaluate environmental relationships.The main factors in this study appear to be pH, nutrient pollution (saprobity and trophic degree) and alkalinity. Within the group of waters which is not influenced by extreme conditions of pH or pollution, there is a discrepancy between waters with low and high alkalinity.The present study confirms that diatoms are useful indicators of pollution and pH, as well documented by several authors. Diatoms are also suitable as biological indicators for alkalinity at least on a regional scale. This offers possibilities for the use of diatoms in monitoring studies, especially in waters that are still under relatively low environmental stress. It also offers opportunities for setting ecological standards, based on the characteristics of non-polluted water bodies. This aspect will be of interest to the water management policy in the province of Overijssel.