共查询到20条相似文献,搜索用时 31 毫秒
1.
Release rates of recently fixed $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ from non-exchangeable interlayer sites in 2:1 silicate minerals were determined for decomposed granite (DG) saprolites from three locations in California, USA. Recently-fixed $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ release from the DG substrate was quantified by extracting diffused $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ with H-resin, as well as a native, annual grass Vulpia microstachys. The $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ release data varied with via the method of extraction, which included H-resin pre-treatments (Na+ or H+) and V. microstachys uptake (mycorrhizal inoculated or uninoculated). After 6 weeks (1008 h), more $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ was recovered from fixed interlayer positions by the H-resins as compared to uptake by V. microstachys. The H+ treated H-resins recovered more released $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ (≈94 mg ${\text{NH}}^{{\text{ + }}}_{{\text{4}}} - {\text{N}}\;{\text{kg}}^{1} $ or (12%) of total fixed $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ ) in two of the three DG samples as compared to the Na+ treated resins, (which recovered ≈70–78 mg ${\text{NH}}^{{\text{ + }}}_{{\text{4}}} - {\text{N}}\;{\text{kg}}^{{{\text{ - 1}}}} $ (or 9–10%) of the total fixed $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ ). The V. microstachys assimilated 8–9% of the total fixed $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ with mycorrhizal inoculum as compared to only 2% without a mycorrhizal inoculum, over the same time period. The fixed $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ release kinetics from the H-resin experiments were most accurately described by first order and power function models, and can be characterized as biphasic using a heterogeneous diffusion model. Uptake of both the 15N and ambient, unlabelled N from the soils was closely related to plant biomass. There was no significant difference in percent of N per unit of biomass between the control and mycorrhizal treatments. The findings presented here indicate that observed, long-term $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ release rates from DG in studies utilizing resins, may overestimate the levels of fixed $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ made available to plants and microorganisms. Additionally, the study suggested that mycorrhizae facilitate the acquisition and plant uptake of fixed $ {\text{NH}}^{{\text{ + }}}_{{\text{4}}} $ , resulting in markedly increased plant biomass production. 相似文献
2.
Levins’s asymmetrical α index quantifies between species overlap over resources more realistically than similar-purpose single-value indices. The associated community-wide \(\bar \alpha\) index expresses the degree of “species packing”. Both indices were formulated upon competing animal (i.e., mobile) organisms and are independent of population densities. However, overlap over resources for nonmobile organisms such as plants may have an impact even below carrying capacity. The proposed \(\hat \alpha\) index, based on Levins’s α index, quantifies spatial overlap for plants integrating information on species spatial distribution and crowding conditions. The \(\hat \alpha\) index is specifically designed for plant distribution data collected in discrete plots with density expressed as percent coverage (%cover) of substratum. We also propose a community-wide \({\hat \alpha_{\text{c}}}\) index, conceptually analogous to \(\bar \alpha\) , but furnished with a measure of dispersion (se \({\hat \alpha_{\text{c}}}\) ). Species importance within the community is inferred from comparisons of pairwise \(\hat \alpha\) ’s with \({\hat \alpha_{\text{c}}}\) . The \(\hat \alpha\) and \({\hat \alpha_{\text{c}}}\) indices correlate closely and exponentially with plant density, and correct apparent over- and underestimations of interaction intensity at low and very high crowding by Levins’s α and \(\bar \alpha\) , respectively. Index application to aquatic plant communities gave results consistent with within-community and general ecological patterns, suggesting a high potential of the proposed \(\hat \alpha\) and \({\hat \alpha_{\text{c}}}\) indices in basic and applied macrophyte ecological studies and management. 相似文献
3.
Anthony P. Farrell Erika J. Eliason Timothy D. Clark Maria F. Steinhausen 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2014,184(7):855-864
While it is well known that O2 is directly removed from the water by skin and gill tissues of fish, the mismatch between O2 removal from water (O2 uptake; \(\dot{V}{\text{O}}_{ 2}\) ) and the O2 delivered to tissues by the primary circulation (O2 consumption; \(\dot{V}{\text{aO}}_{ 2}\) ) has never been measured directly. Using data from four recent studies that simultaneously measured \(\dot{V}{\text{O}}_{ 2}\) and \(\dot{V}{\text{aO}}_{ 2}\) in 2–5 kg Pacific salmon, our analysis revealed that sockeye salmon can remove an additional 12–48 % more O2 from the water than the primary circulation delivers to the systemic tissues. This percentage did not change significantly during swimming activity, a result that contradicts an earlier prediction that the difference should decrease when \(\dot{V}{\text{O}}_{ 2}\) increases during exercise. In resting Chinook salmon, a similar percentage difference in simultaneously measured \(\dot{V}{\text{O}}_{ 2}\) and \(\dot{V}{\text{O}}_{ 2}\) was observed, yet the difference tended to disappear during acute heat stress to a near lethal temperature. These results emphasize that caution should be exercised when using the Fick equation to estimate cardiac output because the overestimate of cardiac output that results from using the Fick equation in Pacific salmon is not small, may not be fixed and may exist in other teleosts. 相似文献
4.
Ken-ichi Tsubota Shigeo Wada Hao Liu 《Biomechanics and modeling in mechanobiology》2014,13(4):735-746
Direct numerical simulations of the mechanics of a single red blood cell (RBC) were performed by considering the nonuniform natural state of the elastic membrane. A RBC was modeled as an incompressible viscous fluid encapsulated by an elastic membrane. The in-plane shear and area dilatation deformations of the membrane were modeled by Skalak constitutive equation, while out-of-plane bending deformation was formulated by the spring model. The natural state of the membrane with respect to in-plane shear deformation was modeled as a sphere ( \(\alpha =0\) ), biconcave disk shape ( \(\alpha =1\) ) and their intermediate shapes ( \(0<\alpha <1\) ) with the nonuniformity parameter \(\alpha \) , while the natural state with respect to out-of-plane bending deformation was modeled as a flat plane. According to the numerical simulations, at an experimentally measured in-plane shear modulus of \(2.5\times 10^{-6}\,\hbox {N}/\hbox {m}\) and an out-of-plane bending rigidity of \(2.0\times 10^{-19}\,\hbox {N}\cdot \hbox {m}\) of the cell membrane, the following results were obtained. (i) The RBC shape at equilibrium was biconcave discoid for \(\alpha >0.22\) and cupped otherwise; (ii) the experimentally measured fluid shear stress at the transition between tumbling and tank-treading motions under shear flow was reproduced for \(0.05<\alpha <0.34\) ; (iii) the elongation deformation of the RBC during tank-treading motion from the simulation was consistent with that from in vitro experiments, irrespective of the \(\alpha \) value. Based on our RBC modeling, the three phenomena (i), (ii), and (iii) were mechanically consistent for \(0.22<\alpha <0.34\) . The condition \(0.05<\alpha <0.22\) precludes a biconcave discoid shape at equilibrium (i); however, it gives appropriate fluid shear stress at the motion transition under shear flow (ii), suggesting that a combined effect of \(\alpha \) and the natural state with respect to out-of-plane bending deformation is necessary for understanding details of the RBC mechanics at equilibrium. Our numerical results demonstrate that moderate nonuniformity in a membrane’s natural state with respect to in-plane shear deformation plays a key role in RBC mechanics. 相似文献
5.
Augmentation of the mechanical properties of connective tissue using ultraviolet (UV) radiation—by targeting collagen cross-linking in the tissue at predetermined UV exposure time \((t)\) and wavelength \((\lambda )\) —has been proposed as a therapeutic method for supporting the treatment for structural-related injuries and pathologies. However, the effects of \(\lambda \) and \(t\) on the tissue elasticity, namely elastic modulus \((E)\) and modulus of resilience \((u_\mathrm{Y})\) , are not entirely clear. We present a thermomechanical framework to reconcile the \(t\) - and \(\lambda \) -related effects on \(E\) and \(u_\mathrm{Y}\) . The framework addresses (1) an energy transfer model to describe the dependence of the absorbed UV photon energy, \(\xi \) , per unit mass of the tissue on \(t\) and \(\lambda \) , (2) an intervening thermodynamic shear-related parameter, \(G\) , to quantify the extent of UV-induced cross-linking in the tissue, (3) a threshold model for the \(G\) versus \(\xi \) relationship, characterized by \(t_\mathrm{C}\) —the critical \(t\) underpinning the association of \(\xi \) with \(G\) —and (4) the role of \(G\) in the tissue elasticity. We hypothesized that \(G\) regulates \(E\) (UV-stiffening hypothesis) and \(u_\mathrm{Y}\) (UV-resilience hypothesis). The framework was evaluated with the support from data derived from tensile testing on isolated ligament fascicles, treated with two levels of \(\lambda \) (365 and 254 nm) and three levels of \(t\) (15, 30 and 60 min). Predictions from the energy transfer model corroborated the findings from a two-factor analysis of variance of the effects of \(t\) and \(\lambda \) treatments. Student’s t test revealed positive change in \(E\) and \(u_\mathrm{Y}\) with increases in \(G\) —the findings lend support to the hypotheses, implicating the implicit dependence of UV-induced cross-links on \(t\) and \(\lambda \) for directing tissue stiffness and resilience. From a practical perspective, the study is a step in the direction to establish a UV irradiation treatment protocol for effective control of exogenous cross-linking in connective tissues. 相似文献
6.
A simple method using the O2 electrode that allows examination of the response of respiration and photosynthesis in leaf slices or algae to anoxia and high light under different temperatures useful for the examination of the interactions among photosynthesis, photorespiration, and respiration is described. The method provides a quantifiable assessment of stress tolerance that also permits us to examine fundamental biochemically and genetically related responses involved in stress tolerance and the cooperation among organelles. Additionally, we demonstrated a role for compounds, such as $ {\text{NO}}^{{\text{ - }}}_{{\text{3}}} $ and oxaloacetate, as protective agents against photoinhibition, and we examined the role of dark adaptation in the activation of photosynthesis and $ {\text{NO}}^{{\text{ - }}}_{{\text{3}}} $ -dependent O2 oxygen evolution. A physiological and ecological role of a dark period (night) in stress tolerance is presented. Utilizing the method to follow changes in such metabolic activities as protein synthesis, protein conformation states, enzymes activity, carbon metabolism, and gene expression at different points during the treatments will be educational. 相似文献
7.
Sandy Adriaenssens Jeroen Staelens Karen Wuyts Roeland Samson Kris Verheyen Pascal Boeckx 《Ecosystems》2012,15(7):1093-1107
Nitrogen (N) retention by tree canopies is believed to be an important process for tree nutrient uptake, and its quantification is a key issue in determining the impact of atmospheric N deposition on forest ecosystems. Due to dry deposition and retention by other canopy elements, the actual uptake and assimilation by the tree canopy is often obscured in throughfall studies. In this study, 15N-labeled solutions ( $ ^{15} {\text{NH}}_{4}^{ + } $ and $ ^{15} {\text{NO}}_{3}^{ - } $ ) were used to assess dissolved inorganic N retention by leaves/needles and twigs of European beech, pedunculate oak, silver birch, and Scots pine saplings. The effects of N form, tree species, leaf phenology, and applied $ {\text{NO}}_{3}^{ - } $ to $ {\text{NH}}_{4}^{ + } $ ratio on the N retention were assessed. Retention patterns were mainly determined by foliar uptake, except for Scots pine. In twigs, a small but significant 15N enrichment was detected for $ {\text{NH}}_{4}^{ + } $ , which was found to be mainly due to physicochemical adsorption to the woody plant surface. The mean $ {{^{15} {\text{NH}}_{4}^{ + } } \mathord{\left/ {\vphantom {{^{15} {\text{NH}}_{4}^{ + } } {^{15} {\text{NO}}_{3}^{ - } }}} \right. \kern-0em} {^{15} {\text{NO}}_{3}^{ - } }} $ retention ratio varied considerably among species and phenological stadia, which indicates that the use of a fixed ratio in the canopy budget model could lead to an over- or underestimation of the total N retention. In addition, throughfall water under each branch was collected and analyzed for $ ^{15} {\text{NH}}_{4}^{ + } $ , $ ^{15} {\text{NO}}_{3}^{ - } $ , and all major ions. Net throughfall of $ ^{15} {\text{NH}}_{4}^{ + } $ was, on average, 20 times higher than the actual retention of $ ^{15} {\text{NH}}_{4}^{ + } $ by the plant material. This difference in $ ^{15} {\text{NH}}_{4}^{ + } $ retention could not be attributed to pools and fluxes measured in this study. The retention of $ ^{15} {\text{NH}}_{4}^{ + } $ was correlated with the net throughfall of K+, Mg2+, Ca2+, and weak acids during leaf development and the fully leafed period, while no significant relationships were found for $ ^{15} {\text{NO}}_{3}^{ - } $ retention. This suggests that the main driving factors for $ {\text{NH}}_{4}^{ + } $ retention might be ion exchange processes during the start and middle of the growing season and passive diffusion at leaf senescence. Actual assimilation or abiotic uptake of N through leaves and twigs was small in this study, for example, 1–5% of the applied dissolved 15N, indicating that the impact of canopy N retention from wet deposition on forest productivity and carbon sequestration is likely limited. 相似文献
8.
Osvaldo A. Martin Myriam E. Villegas Jorge A. Vila Harold A. Scheraga 《Journal of biomolecular NMR》2010,46(3):217-225
Cysteines possess a unique property among the 20 naturally occurring amino acids: it can be present in proteins in either the reduced or oxidized form, and can regulate the activity of some proteins. Consequently, to augment our previous treatment of the other types of residues, the $ {}^{13}{\text{C}}^{{{\upalpha}}} $ and $ {}^{13}{\text{C}}^{{{\upbeta}}} $ chemical shifts of 837 cysteines in disulfide-bonded cystine from a set of seven non-redundant proteins, determined by X-ray crystallography and NMR spectroscopy, were computed at the DFT level of theory. Our results indicate that the errors between observed and computed $ {}^{13}{\text{C}}^{{{\upalpha}}} $ chemical shifts of such oxidized cysteines can be attributed to several effects such as: (a) the quality of the NMR-determined models, as evaluated by the conformational-average (ca) rmsd value; (b) the existence of high B-factor or crystal-packing effects for the X-ray-determined structures; (c) the dynamics of the disulfide bonds in solution; and (d) the differences in the experimental conditions under which the observed $ {}^{13}{\text{C}}^{{{\upalpha}}} $ chemical shifts and the protein models were determined by either X-ray crystallography or NMR-spectroscopy. These quantum-chemical-based calculations indicate the existence of two, almost non-overlapped, basins for the oxidized and reduced ?SH $ {}^{13}{\text{C}}^{{{\upbeta}}} $ , but not for the $ {}^{13}{\text{C}}^{{{\upalpha}}} $ , chemical shifts, in good agreement with the observation of 375 $ {}^{13}{\text{C}}^{{{\upalpha}}} $ and 337 $ {}^{13}{\text{C}}^{{{\upbeta}}} $ resonances from 132 proteins by Sharma and Rajarathnam (2000). Overall, our results indicate that explicit consideration of the disulfide bonds is a necessary condition for an accurate prediction of $ {}^{13}{\text{C}}^{{{\upalpha}}} $ and $ {}^{13}{\text{C}}^{{{\upbeta}}} $ chemical shifts of cysteines in cystines. 相似文献
9.
Pascal R. Buenzli Peter Pivonka David W. Smith 《Biomechanics and modeling in mechanobiology》2014,13(1):185-203
Bone remodelling is carried out by ‘bone multicellular units’ ( $\text{ BMU }$ s) in which active osteoclasts and active osteoblasts are spatially and temporally coupled. The refilling of new bone by osteoblasts towards the back of the $\text{ BMU }$ occurs at a rate that depends both on the number of osteoblasts and on their secretory activity. In cortical bone, a linear phenomenological relationship between matrix apposition rate and $\text{ BMU }$ cavity radius is found experimentally. How this relationship emerges from the combination of complex, nonlinear regulations of osteoblast number and secretory activity is unknown. Here, we extend our previous mathematical model of cell development within a single cortical $\text{ BMU }$ to investigate how osteoblast number and osteoblast secretory activity vary along the $\text{ BMU }$ ’s closing cone. The mathematical model is based on biochemical coupling between osteoclasts and osteoblasts of various maturity and includes the differentiation of osteoblasts into osteocytes and bone lining cells, as well as the influence of $\text{ BMU }$ cavity shrinkage on osteoblast development and activity. Matrix apposition rates predicted by the model are compared with data from tetracycline double labelling experiments. We find that the linear phenomenological relationship observed in these experiments between matrix apposition rate and $\text{ BMU }$ cavity radius holds for most of the refilling phase simulated by our model, but not near the start and end of refilling. This suggests that at a particular bone site undergoing remodelling, bone formation starts and ends rapidly, supporting the hypothesis that osteoblasts behave synchronously. Our model also suggests that part of the observed cross-sectional variability in tetracycline data may be due to different bone sites being refilled by $\text{ BMU }$ s at different stages of their lifetime. The different stages of a $\text{ BMU }$ ’s lifetime (such as initiation stage, progression stage, and termination stage) depend on whether the cell populations within the $\text{ BMU }$ are still developing or have reached a quasi-steady state whilst travelling through bone. We find that due to their longer lifespan, active osteoblasts reach a quasi-steady distribution more slowly than active osteoclasts. We suggest that this fact may locally enlarge the Haversian canal diameter (due to a local lack of osteoblasts compared to osteoclasts) near the $\text{ BMU }$ ’s point of origin. 相似文献
10.
Gretchen R. Miles Myron J. Mitchell Bernhard Mayer Gene Likens Jeffrey Welker 《Biogeochemistry》2012,111(1-3):443-454
In response to decreasing atmospheric emissions of sulfur (S) since the 1970s there has been a concomitant decrease in S deposition to watersheds in the Northeastern U.S. Previous study at the Hubbard Brook Experimental Forest, NH (USA) using chemical and isotopic analyzes ( $ \delta^{34} {\text{S}}_{{{\text{SO}}_{4} }} $ ) combined with modeling has suggested that there is an internal source of S within these watersheds that results in a net loss of S via sulfate in drainage waters. The current study expands these previous investigations by the utilization of δ18O analyzes of precipitation sulfate and streamwater sulfate. Archived stream and bulk precipitation samples at the Hubbard Brook Experimental Forest from 1968–2004 were analyzed for stable oxygen isotope ratios of sulfate ( $ \delta^{18} {\text{O}}_{{{\text{SO}}_{4} }} $ ). Overall decreasing temporal trends and seasonally low winter values of $ \delta^{18} {\text{O}}_{{{\text{SO}}_{4} }} $ in bulk precipitation are most likely attributed to similar trends in precipitation $ \delta^{18} {\text{O}}_{{{\text{H}}_{2} {\text{O}}}} $ values. Regional climate trends and changes in temperature control precipitation $ \delta^{18} {\text{O}}_{{{\text{H}}_{2} {\text{O}}}} $ values that are reflected in the $ \delta^{18} {\text{O}}_{{{\text{SO}}_{4} }} $ values of precipitation. The significant relationship between ambient temperature and the $ \delta^{18} {\text{O}}_{{{\text{H}}_{2} {\text{O}}}} $ values of precipitation is shown from a nearby site in Ottawa, Ontario (Canada). Although streamwater $ \delta^{18} {\text{O}}_{{{\text{SO}}_{4} }} $ values did not reveal temporal trends, a large difference between precipitation and streamwater $ \delta^{18} {\text{O}}_{{{\text{SO}}_{4} }} $ values suggest the importance of internal cycling of S especially through the large organic S pool and the concomitant effect on the $ \delta^{18} {\text{O}}_{{{\text{SO}}_{4} }} $ values in drainage waters. 相似文献
11.
Cyclic AMP is important for the resolution of inflammation, as it promotes anti-inflammatory signaling in several immune cell lines. In this paper, we present an immune cell specific model of the cAMP signaling cascade, paying close attention to the specific isoforms of adenylyl cyclase (AC) and phosphodiesterase that control cAMP production and degradation, respectively, in these cells. The model describes the role that G protein subunits, including G \(\alpha _s\) , G \(\alpha _i\) , and G \(\beta \gamma \) , have in regulating cAMP production. Previously, G \(\alpha _i\) activation has been shown to increase the level of cAMP in certain immune cell types. This increase in cAMP is thought to be mediated by \(\beta \gamma \) subunits which are released upon G \(\alpha \) activation and can directly stimulate specific isoforms of AC. We conduct numerical experiments in order to explore the mechanisms through which G \(\alpha _i\) activation can increase cAMP production. An important conclusion of our analysis is that the relative abundance of different G protein subunits is an essential determinant of the cAMP profile in immune cells. In particular, our model predicts that limited availability of \(\beta \gamma \) subunits may both \((i)\) enable immune cells to link inflammatory G \(\alpha _i\) signaling to anti-inflammatory cAMP production thereby creating a balanced immune response to stimulation with low concentrations of PGE2, and \((ii)\) prohibit robust anti-inflammatory cAMP signaling in response to stimulation with high concentrations of PGE2. 相似文献
12.
Jane S. Murray Zenaida Peralta-Inga Shields Pat Lane Laura Macaveiu Felipe A. Bulat 《Journal of molecular modeling》2013,19(7):2825-2833
In a continuing effort to further explore the use of the average local ionization energy $ \overline{\mathrm{I}}\left( \mathbf{r} \right) $ as a computational tool, we have investigated how well $ \overline{\mathrm{I}}\left( \mathbf{r} \right) $ computed on molecular surfaces serves as a predictive tool for identifying the sites of the more reactive electrons in several nonplanar defect-containing model graphene systems, each containing one or more pentagons. They include corannulene (C20H10), two inverse Stone-Thrower-Wales defect-containing structures C26H12 and C42H16, and a nanotube cap model C22H6, whose end is formed by three fused pentagons. Coronene (C24H12) has been included as a reference planar defect-free graphene model. We have optimized the structures of these systems as well as several monohydrogenated derivatives at the B3PW91/6-31G* level, and have computed their $ \overline{\mathrm{I}}\left( \mathbf{r} \right) $ on molecular surfaces corresponding to the 0.001 au, 0.003 au and 0.005 au contours of the electronic density. We find that (1) the convex sides of the interior carbons of the nonplanar models are more reactive than the concave sides, and (2) the magnitudes of the lowest $ \overline{\mathrm{I}}\left( \mathbf{r} \right) $ surface minima (the $ {{\overline{\mathrm{I}}}_{{\mathrm{S}\text{,}\min }}} $ ) correlate well with the interaction energies for hydrogenation at these sites. These $ {{\overline{\mathrm{I}}}_{{\mathrm{S}\text{,}\min }}} $ values decrease in magnitude as the nonplanarity of the site increases, consistent with earlier studies. A practical benefit of the use of $ \overline{\mathrm{I}}\left( \mathbf{r} \right) $ is that a single calculation suffices to characterize the numerous sites on a large molecular system, such as graphene and defect-containing graphene models. Figure
Convex 0.001 au molecular surface of hydrogenated inverse Stone-Thrower-Wales defect-containing model 4H, with the hydrogen attached to one of the central carbons fusing the two pentagons 相似文献
13.
14.
Xu Pang Xing-Zhong Yuan Zhen-Dong Cao Shi-Jian Fu 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2013,183(1):99-108
To investigate the effects of temperature and exercise training on swimming performance in juvenile qingbo (Spinibarbus sinensis), we measured the following: (1) the resting oxygen consumption rate $ \left( {{\dot{\text{M}}\text{O}}_{{ 2 {\text{rest}}}} } \right) $ , critical swimming speed (U crit) and active oxygen consumption rate $ \left( {{\dot{\text{M}}\text{O}}_{{ 2 {\text{active}}}} } \right) $ of fish at acclimation temperatures of 10, 15, 20, 25 and 30 °C and (2) the $ \dot{M}{\text{O}}_{{ 2 {\text{rest}}}} $ , U crit and $ \dot{M}{\text{O}}_{{ 2 {\text{active}}}} $ of both exercise-trained (exhaustive chasing training for 14 days) and control fish at both low and high acclimation temperatures (15 and 25 °C). The relationship between U crit and temperature (T) approximately followed a bell-shaped curve as temperature increased: U crit = 8.21/{1 + [(T ? 27.2)/17.0]2} (R 2 = 0.915, P < 0.001, N = 40). The optimal temperature for maximal U crit (8.21 BL s?1) in juvenile qingbo was 27.2 °C. Both the $ \dot{M}{\text{O}}_{{ 2 {\text{active}}}} $ and the metabolic scope (MS, $ \dot{M}{\text{O}}_{{ 2 {\text{active}}}} - \dot{M}{\text{O}}_{{ 2 {\text{rest}}}} $ ) of qingbo increased with temperature from 10 to 25 °C (P < 0.05), but there were no significant differences between fish acclimated to 25 and 30 °C. The relationships between $ \dot{M}{\text{O}}_{{ 2 {\text{active}}}} $ or MS and temperature were described as $ {\dot{\text{M}}\text{O}}_{{ 2 {\text{active}}}} = 1,214.29/\left\{ {1 + \left[ {\left( {T - 28.8} \right)/10.6} \right]^{2} } \right\}\;\left( {R^{2} = 0.911,\;P < 0.001,\;N = 40} \right) $ and MS = 972.67/{1 + [(T ? 28.0)/9.34]2} (R 2 = 0.878, P < 0.001, N = 40). The optimal temperatures for $ \dot{M}{\text{O}}_{{ 2 {\text{active}}}} $ and MS in juvenile qingbo were 28.8 and 28.0 °C, respectively. Exercise training resulted in significant increases in both U crit and $ \dot{M}{\text{O}}_{{ 2 {\text{active}}}} $ at a low temperature (P < 0.05), but training exhibited no significant effect on either U crit or $ \dot{M}{\text{O}}_{{ 2 {\text{active}}}} $ at a high temperature. These results suggest that exercise training had different effects on swimming performance at different temperatures. These differences may be related to changes in aerobic metabolic capability, arterial oxygen delivery, available dissolved oxygen, imbalances in ion fluxes and stimuli to remodel tissues with changes in temperature. 相似文献
15.
Hannes Uecke Johannes Müller Burkhard A. Hense 《Bulletin of mathematical biology》2014,76(7):1727-1746
Quorum sensing is a wide-spread mode of cell–cell communication among bacteria in which cells release a signalling substance at a low rate. The concentration of this substance allows the bacteria to gain information about population size or spatial confinement. We consider a model for \(N\) cells which communicate with each other via a signalling substance in a diffusive medium with a background flow. The model consists of an initial boundary value problem for a parabolic PDE describing the exterior concentration \(u\) of the signalling substance, coupled with \(N\) ODEs for the masses \(a_i\) of the substance within each cell. The cells are balls of radius \(R\) in \(\mathbb {R} ^3\) , and under some scaling assumptions we formally derive an effective system of \(N\) ODEs describing the behaviour of the cells. The reduced system is then used to study the effect of flow on communication in general, and in particular for a number of geometric configurations. 相似文献
16.
Christoph Wiedemann Peter Bellstedt Christian Herbst Matthias Görlach Ramadurai Ramachandran 《Journal of biomolecular NMR》2014,59(4):211-217
A procedure for the simultaneous acquisition of {HNCOCANH & HCCCONH} chemical shift correlation spectra employing sequential \(^{1}\hbox {H}\) data acquisition for moderately sized proteins is presented. The suitability of the approach for obtaining sequential resonance assignments, including complete \(^{15}\hbox {N}{},\, ^{1}\hbox {H}{}^{N},\, ^{13}\hbox {CO}{},\, ^{13}\hbox {C}^{\alpha }{},\, ^{13}\hbox {C}^{\beta }\) and \(^{1}\hbox {H}^{\alpha }\) chemical shift information, is demonstrated experimentally for a \(^{13}\hbox {C}\) and \(^{15}\hbox {N}\) labelled sample of the C-terminal winged helix (WH) domain of the minichromosome maintenance (MCM) complex of Sulfolobus solfataricus. The chemical shift information obtained was used to calculate the global fold of this winged helix domain via CS-Rosetta. This demonstrates that our procedure provides a reliable and straight-forward protocol for a quick global fold determination of moderately-sized proteins. 相似文献
17.
Mohamed Helal Erwan Hingant Laurent Pujo-Menjouet Glenn F. Webb 《Journal of mathematical biology》2014,69(5):1207-1235
We introduce a mathematical model of the in vivo progression of Alzheimer’s disease with focus on the role of prions in memory impairment. Our model consists of differential equations that describe the dynamic formation of \(\upbeta \) -amyloid plaques based on the concentrations of A \(\upbeta \) oligomers, PrPC proteins, and the A \(\upbeta \) - \(\times \) -PrPCcomplex, which are hypothesized to be responsible for synaptic toxicity. We prove the well-posedness of the model and provided stability results for its unique equilibrium, when the polymerization rate of \(\upbeta \) -amyloid is constant and also when it is described by a power law. 相似文献
18.
We describe a method that will reconstruct an unrooted binary phylogenetic level-1 network on \(n\) taxa from the set of all quartets containing a certain fixed taxon, in \(O(n^3)\) time. We also present a more general method which can handle more diverse quartet data, but which takes \(O(n^6)\) time. Both methods proceed by solving a certain system of linear equations over the two-element field \(\mathrm{GF}(2)\) . For a general dense quartet set, i.e. a set containing at least one quartet on every four taxa, our \(O(n^6)\) algorithm constructs a phylogenetic level-1 network consistent with the quartet set if such a network exists and returns an \(O(n^2)\) -sized certificate of inconsistency otherwise. This answers a question raised by Gambette, Berry and Paul regarding the complexity of reconstructing a level-1 network from a dense quartet set, and more particularly regarding the complexity of constructing a cyclic ordering of taxa consistent with a dense quartet set. 相似文献
19.
The unusual ??-halogen bond interactions are investigated between $ \left( {\hbox{BNN}} \right)_3^{+} $ and X1X2 (X1, X2?=?F, Cl, Br) employing MP2 at 6-311?+?G(2d) and aug-cc-pVDZ levels according to the ??CP (counterpoise) corrected potential energy surface (PES)?? method. The order of the ??-halogen bond interactions and stabilities of the complexes are obtained to be $ \left( {\hbox{BNN}} \right)_3^{+} \ldots {{\hbox{F}}_2} < \left( {\hbox{BNN}} \right)_3^{+} \ldots {\hbox{ClF < }}\left( {\hbox{BNN}} \right)_3^{+} \ldots {\hbox{C}}{{\hbox{l}}_2} < \left( {\hbox{BNN}} \right)_3^{+} \ldots {\hbox{BrCl}}\quad { < }\quad \left( {\hbox{BNN}} \right)_3^{+} \ldots {\hbox{B}}{{\hbox{r}}_2}\quad { < }\quad \left( {\hbox{BNN}} \right)_3^{+} \ldots {\hbox{BrF}}{.} $ at MP2/aug-cc-pVDZ level. The analyses of the Mulliken charge transfer, natural bond orbital (NBO), atoms in molecules (AIM) theory and electron density shifts reveal that the nature of the ??-halogen bond interaction in the complexes of ClF, BrF and BrCl might partly be charge transfer from the delocalized ??-HOMO orbital of $ \left( {\hbox{BNN}} \right)_3^{+} $ to X1X2. This result suggests that the positive aromatic ring $ \left( {\hbox{BNN}} \right)_3^{+} $ might act as a ??-electron donor to form the ??-halogen bond. Figure
Shifts of electron density as a result of formation of the complex. The unusual ??-halogen interactions are found between (BNN)3 + and X1X2 (X1, X2=F, Cl, Br) employing MP2 method at 6-311+G(2d) and aug-cc-pVDZ levels according to the ??CP-corrected PES)?? method. The analyses of the Mulliken charge transfer, NBO, AIM and electron density shifts reveal that the nature of the ??-halogen bond interaction in the complexes of ClF, BrF and BrCl might partly be charge transfer from the delocalized ??-HOMO orbital of (BNN)3 + to X1X2. (BNN)3 + might be as ??-electron donor to form the ??-halogen bond. 相似文献
20.
Songbai Ji Wei Zhao Zhigang Li Thomas W. McAllister 《Biomechanics and modeling in mechanobiology》2014,13(5):1121-1136
Both linear \((\mathbf{a}_{\mathrm{lin}})\) and rotational \((\mathbf{a}_{\mathrm{rot}} )\) accelerations contribute to head impacts on the field in contact sports; however, they are often isolated in injury studies. It is critical to evaluate the feasibility of estimating brain responses using isolated instead of full degrees-of-freedom (DOFs) accelerations. In this study, we investigated the sensitivities of regional brain strain-related responses to resultant \(\mathbf{a}_{\mathrm{lin}}\) and \(\mathbf{a}_{\mathrm{rot}}\) as well as the relative contributions of these acceleration components to the responses via random sampling and linear regression using parameterized, triangulated head impacts with kinematic variable values based on on-field measurements. Two independently established and validated finite element models of the human head were employed to evaluate model-consistency and dependency in results: the Dartmouth Head Injury Model and Simulated Injury Monitor. For the majority of the brain, volume-weighted regional peak strain, strain rate, and von Mises stress accumulated from the simulation significantly correlated with the product of the magnitude and duration of \(\mathbf{a}_{\mathrm{rot}}\) , or effectively, the rotational velocity, but not to \(\mathbf{a}_{\mathrm{lin}}\) . Responses from \(\mathbf{a}_{\mathrm{rot}}\) -only were comparable to the full-DOF counterparts especially when normalized by injury-causing thresholds (e.g., volume fractions of large differences virtually diminished (i.e., \(<\) 1 %) at typical difference percentage levels of 1–4 % on average). These model-consistent results support the inclusion of both rotational acceleration magnitude and duration into kinematics-based injury metrics and demonstrate the feasibility of estimating strain-related responses from isolated \(\mathbf{a}_{\mathrm{rot}}\) for analyses of strain-induced injury relevant to contact sports without significant loss of accuracy, especially for the cerebrum. 相似文献