共查询到20条相似文献,搜索用时 31 毫秒
1.
Ralf Uptmoor Tobias Schrag Hartmut Stützel Elisabeth Esch 《Molecular breeding : new strategies in plant improvement》2008,21(2):205-216
Studying quantitative traits is complicated due to genotype by environment interactions. One strategy to overcome these difficulties
is to combine quantitative trait loci (QTL) and ecophysiological models, e.g. by identifying QTLs for the response curves
of adaptive traits to influential environmental factors. A B. oleracea DH-population segregating for time to flowering was cultivated at different temperature regimes. Composite interval mapping
was carried out on the three parameters of a model describing time to flowering as a function of temperature, i.e. on the
intercept and slope of the response of time to floral induction to temperature and on the duration from transition to flowering.
The additive effects of QTLs detected for the parameters have been used to estimate time to floral induction and flowering
in the B. oleracea DH-population. The combined QTL and crop model explained 66% of the phenotypic variation for time to floral induction and
56% of the phenotypic variation for time to flowering. Estimation of time to floral induction and flowering based on environment
specific QTLs explained 61 and 41% of the phenotypic variation. Results suggest that flowering time can be predicted effectively
by coupling QTL and crop models and that using crop modelling tools for QTL analysis increases the power of QTL detection. 相似文献
2.
A longitudinal approach is proposed to map QTL affecting function-valued traits and to estimate their effect over time. The method is based on fitting mixed random regression models. The QTL allelic effects are modelled with random coefficient parametric curves and using a gametic relationship matrix. A simulation study was conducted in order to assess the ability of the approach to fit different patterns of QTL over time. It was found that this longitudinal approach was able to adequately fit the simulated variance functions and considerably improved the power of detection of time-varying QTL effects compared to the traditional univariate model. This was confirmed by an analysis of protein yield data in dairy cattle, where the model was able to detect QTL with high effect either at the beginning or the end of the lactation, that were not detected with a simple 305 day model. 相似文献
3.
An interval quantitative trait locus (QTL) mapping method for complex polygenic diseases (as binary traits) showing QTL by environment interactions (QEI) was developed for outbred populations on a within-family basis. The main objectives, within the above context, were to investigate selection of genetic models and to compare liability or generalized interval mapping (GIM) and linear regression interval mapping (RIM) methods. Two different genetic models were used: one with main QTL and QEI effects (QEI model) and the other with only a main QTL effect (QTL model). Over 30 types of binary disease data as well as six types of continuous data were simulated and analysed by RIM and GIM. Using table values for significance testing, results show that RIM had an increased false detection rate (FDR) for testing interactions which was attributable to scale effects on the binary scale. GIM did not suffer from a high FDR for testing interactions. The use of empirical thresholds, which effectively means higher thresholds for RIM for testing interactions, could repair this increased FDR for RIM, but such empirical thresholds would have to be derived for each case because the amount of FDR depends on the incidence on the binary scale. RIM still suffered from higher biases (15-100% over- or under-estimation of true values) and high standard errors in QTL variance and location estimates than GIM for QEI models. Hence GIM is recommended for disease QTL mapping with QEI. In the presence of QEI, the model including QEI has more power (20-80% increase) to detect the QTL when the average QTL effect is small (in a situation where the model with a main QTL only is not too powerful). Top-down model selection is proposed in which a full test for QEI is conducted first and then the model is subsequently simplified. Methods and results will be applicable to human, plant and animal QTL mapping experiments. 相似文献
4.
A mixed-model approach to mapping quantitative trait loci in barley on the basis of multiple environment data 总被引:16,自引:0,他引:16
Piepho HP 《Genetics》2000,156(4):2043-2050
In this article, I propose a mixed-model method to detect QTL with significant mean effect across environments and to characterize the stability of effects across multiple environments. I demonstrate the method using the barley dataset by the North American Barley Genome Mapping Project. The analysis raises the need for mixed modeling in two different ways. First, it is reasonable to regard environments as a random sample from a population of target environments. Thus, environmental main effects and QTL-by-environment interaction effects are regarded as random. Second, I expect a genetic correlation among pairs of environments caused by undetected QTL. I show how random QTL-by-environment effects as well as genetic correlations are straightforwardly handled in a mixed-model framework. The main advantage of this method is the ability to assess the stability of QTL effects. Moreover, the method allows valid statistical inferences regarding average QTL effects. 相似文献
5.
When three traits make a line: evolution of phenotypic plasticity and genetic assimilation through linear reaction norms in stochastic environments
下载免费PDF全文
![点击此处可从《Journal of evolutionary biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Genetic assimilation emerges from selection on phenotypic plasticity. Yet, commonly used quantitative genetics models of linear reaction norms considering intercept and slope as traits do not mimic the full process of genetic assimilation. We argue that intercept–slope reaction norm models are insufficient representations of genetic effects on linear reaction norms and that considering reaction norm intercept as a trait is unfortunate because the definition of this trait relates to a specific environmental value (zero) and confounds genetic effects on reaction norm elevation with genetic effects on environmental perception. Instead, we suggest a model with three traits representing genetic effects that, respectively, (i) are independent of the environment, (ii) alter the sensitivity of the phenotype to the environment and (iii) determine how the organism perceives the environment. The model predicts that, given sufficient additive genetic variation in environmental perception, the environmental value at which reaction norms tend to cross will respond rapidly to selection after an abrupt environmental change, and eventually becomes equal to the new mean environment. This readjustment of the zone of canalization becomes completed without changes in genetic correlations, genetic drift or imposing any fitness costs of maintaining plasticity. The asymptotic evolutionary outcome of this three‐trait linear reaction norm generally entails a lower degree of phenotypic plasticity than the two‐trait model, and maximum expected fitness does not occur at the mean trait values in the population. 相似文献
6.
Variance component models are commonly used to detect quantitative trait loci (QTL) in general pedigrees. The variance-covariance structure of the random QTL effect is given by the identity by descent (IBD) between genotypes. Epistatic effects have previously been modeled, both for unlinked and linked loci, as a random effect with a variance-covariance structure given by the Hadamard product between the IBD matrices of the direct QTL effects. In the original papers, the model was given but not derived. Here, we identify the underlying assumptions of this previously proposed model. It assumes that either an unlinked QTL or a fully informative marker (i.e., all marker alleles are unique in the base generation) is located between the loci. We discuss the need of developing a general algorithm to estimate the variance-covariance structure of the random epistatic effect for linked loci. 相似文献
7.
Vargas M van Eeuwijk FA Crossa J Ribaut JM 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(6):1009-1023
The study of QTL × environment interaction (QEI) is important for understanding genotype × environment interaction (GEI) in
many quantitative traits. For modeling GEI and QEI, factorial regression (FR) models form a powerful class of models. In FR
models, covariables (contrasts) defined on the levels of the genotypic and/or environmental factor(s) are used to describe
main effects and interactions. In FR models for QTL expression, considerable numbers of genotypic covariables can occur as
for each putative QTL an additional covariable needs to be introduced. For large numbers of genotypic and/or environmental
covariables, least square estimation breaks down and partial least squares (PLS) estimation procedures become an attractive
alternative. In this paper we develop methodology for analyzing QEI by FR for estimating effects and locations of QTLs and
QEI and interpreting QEI in terms of environmental variables. A randomization test for the main effects of QTLs and QEI is
presented. A population of F2 derived F3 families was evaluated in eight environments differing in drought stress and soil nitrogen content and the traits yield and
anthesis silking interval (ASI) were measured. For grain yield, chromosomes 1 and 10 showed significant QEI, whereas in chromosomes
3 and 8 only main effect QTLs were observed. For ASI, QTL main effects were observed on chromosomes 1, 2, 6, 8, and 10, whereas
QEI was observed only on chromosome 8. The assessment of the QEI at chromosome 1 for grain yield showed that the QTL main
effect explained 35.8% of the QTL + QEI variability, while QEI explained 64.2%. Minimum temperature during flowering time
explained 77.6% of the QEI. The QEI analysis at chromosome 10 showed that the QTL main effect explained 59.8% of the QTL + QEI
variability, while QEI explained 40.2%. Maximum temperature during flowering time explained 23.8% of the QEI. Results of this
study show the possibilities of using FR for mapping QTL and for dissecting QEI in terms of environmental variables. PLS regression
is efficient in accounting for background noise produced by other QTLs. 相似文献
8.
Nora Falconieri Ben Van Calster Dirk Timmerman Laure Wynants 《Biometrical journal. Biometrische Zeitschrift》2020,62(4):932-944
Although multicenter data are common, many prediction model studies ignore this during model development. The objective of this study is to evaluate the predictive performance of regression methods for developing clinical risk prediction models using multicenter data, and provide guidelines for practice. We compared the predictive performance of standard logistic regression, generalized estimating equations, random intercept logistic regression, and fixed effects logistic regression. First, we presented a case study on the diagnosis of ovarian cancer. Subsequently, a simulation study investigated the performance of the different models as a function of the amount of clustering, development sample size, distribution of center-specific intercepts, the presence of a center-predictor interaction, and the presence of a dependency between center effects and predictors. The results showed that when sample sizes were sufficiently large, conditional models yielded calibrated predictions, whereas marginal models yielded miscalibrated predictions. Small sample sizes led to overfitting and unreliable predictions. This miscalibration was worse with more heavily clustered data. Calibration of random intercept logistic regression was better than that of standard logistic regression even when center-specific intercepts were not normally distributed, a center-predictor interaction was present, center effects and predictors were dependent, or when the model was applied in a new center. Therefore, to make reliable predictions in a specific center, we recommend random intercept logistic regression. 相似文献
9.
In statistical models, a quantitative trait locus (QTL) effect has been incorporated either as a fixed or as a random term, but, up to now, it has been mainly considered as a time-independent variable. However, for traits recorded repeatedly, it is very interesting to investigate the variation of QTL over time. The major goal of this study was to estimate the position and effect of QTL for milk, fat, protein yields and for somatic cell score based on test day records, while testing whether the effects are constant or variable throughout lactation. The analysed data consisted of 23 paternal half-sib families (716 daughters of 23 sires) of Chinese Holstein-Friesian cattle genotyped at 14 microsatellites located in the area of the casein loci on BTA6. A sequence of three models was used: (i) a lactation model, (ii) a random regression model with a QTL constant in time and (iii) a random regression model with a QTL variable in time. The results showed that, for each production trait, at least one significant QTL exists. For milk and protein yields, the QTL effect was variable in time, while for fat yield, each of the three models resulted in a significant QTL effect. When a QTL is incorporated into a model as a constant over time, its effect is averaged over lactation stages and may, thereby, be difficult or even impossible to be detected. Our results showed that, in such a situation, only a longitudinal model is able to identify loci significantly influencing trait variation. 相似文献
10.
Interval mapping by simple regression is a powerful method for the detection of quantitative trait loci (QTLs) in line crosses such as F2 populations. Due to the ease of computation of the regression approach, relatively complex models with multiple fixed effects, interactions between QTLs or between QTLs and fixed effects can easily be accommodated. However, polygenic effects, which are not targeted in QTL analysis, cannot be treated as random effects in a least squares analysis. In a cross between true inbred lines this is of no consequence, as the polygenic effect contributes just to the residual variance. In a cross between outbred lines, however, if a trait has high polygenic heritability, the additive polygenic effect has a large influence on variation in the population. Here we extend the fixed model for the regression interval mapping method to a mixed model using an animal model. This makes it possible to use not only the observations from progeny (e.g. F2), but also those from the parents (F1) to evaluate QTLs and polygenic effects. We show how the animal model using parental observations can be applied to an outbred cross and so increase the power and accuracy of QTL analysis. Three estimation methods, i.e. regression and an animal model either with or without parental observations, are applied to simulated data. The animal model using parental observations is shown to have advantages in estimating QTL position and additive genotypic value, especially when the polygenic heritability is large and the number of progeny per parent is small. 相似文献
11.
Evidence that a single gene with gender- and age-dependent effects influences systolic blood pressure determination in a population-based sample. 总被引:9,自引:4,他引:5
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A biometrical study was carried out to evaluate the role of genetic variation in determining interindividual differences in systolic blood pressure (SBP) in the population at large. SBP was measured in 1,266 Caucasian individuals in 278 pedigrees ascertained through children enrolled in the Rochester, MN, school system. The sample included 646 males and 620 females 550 years of age and not taking antihypertensive medication or oral contraceptives. Complex segregation analysis was first applied to these data by using a regression model for age, in which the intercept was gender and ousiotype specific but in which the slope was only gender specific. When the slope was independent of ousiotype, neither variation at a single gene combined with polygenic effects (mixed genetic model) nor variation in a single environmental factor combined with polygenetic effects (mixed environmental model) explained the distribution of SBP in this sample. However, when the regression model for age allowed both the intercept and slope to be gender and ousiotype specific, the mixed environmental model was rejected whereas the mixed genetic model was not. These results suggest that variability in SBP may be influenced by major effects of allelic variation at a single gene that are both gender and age dependent. This study (1) suggests that particular genotypes determined by a single gene are associated with a steeper increase of SBP with age among males and females 550 years of age in the general population and (2) illustrates the need to consider models that more realistically represent the relationship between genotypic variability and phenotypic variability, to understand the genetics of human quantitative traits. 相似文献
12.
Using probe genotypes to dissect QTL × environment interactions for grain yield components in winter wheat 总被引:1,自引:0,他引:1
Bing Song Zheng Jacques Le Gouis Martine Leflon Wen Ying Rong Anne Laperche Maryse Brancourt-Hulmel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(8):1501-1517
13.
《Animal : an international journal of animal bioscience》2018,12(9):1807-1814
Longer-lived cows tend to be more profitable and the stayability trait is a selection criterion correlated to longevity. An alternative to the traditional approach to evaluate stayability is its definition based on consecutive calvings, whose main advantage is the more accurate evaluation of young bulls. However, no study using this alternative approach has been conducted for Zebu breeds. Therefore, the objective of this study was to compare linear random regression models to fit stayability to consecutive calvings of Guzerá, Nelore and Tabapuã cows and to estimate genetic parameters for this trait in the respective breeds. Data up to the eighth calving were used. The models included the fixed effects of age at first calving and year-season of birth of the cow and the random effects of contemporary group, additive genetic, permanent environmental and residual. Random regressions were modeled by orthogonal Legendre polynomials of order 1 to 4 (2 to 5 coefficients) for contemporary group, additive genetic and permanent environmental effects. Using Deviance Information Criterion as the selection criterion, the model with 4 regression coefficients for each effect was the most adequate for the Nelore and Tabapuã breeds and the model with 5 coefficients is recommended for the Guzerá breed. For Guzerá, heritabilities ranged from 0.05 to 0.08, showing a quadratic trend with a peak between the fourth and sixth calving. For the Nelore and Tabapuã breeds, the estimates ranged from 0.03 to 0.07 and from 0.03 to 0.08, respectively, and increased with increasing calving number. The additive genetic correlations exhibited a similar trend among breeds and were higher for stayability between closer calvings. Even between more distant calvings (second v. eighth), stayability showed a moderate to high genetic correlation, which was 0.77, 0.57 and 0.79 for the Guzerá, Nelore and Tabapuã breeds, respectively. For Guzerá, when the models with 4 or 5 regression coefficients were compared, the rank correlations between predicted breeding values for the intercept were always higher than 0.99, indicating the possibility of practical application of the least parameterized model. In conclusion, the model with 4 random regression coefficients is recommended for the genetic evaluation of stayability to consecutive calvings in Zebu cattle. 相似文献
14.
The effects of quantitative trait loci (QTL) on phenotypic development may depend on the environment (QTL x environment interaction), other QTL (genetic epistasis), or both. In this article, we present a new statistical model for characterizing specific QTL that display environment-dependent genetic expressions and genotype x environment interactions for developmental trajectories. Our model was derived within the maximum-likelihood-based mixture model framework, incorporated by biologically meaningful growth equations and environment-dependent genetic effects of QTL, and implemented with the EM algorithm. With this model, we can characterize the dynamic patterns of genetic effects of QTL governing growth curves and estimate the global effect of the underlying QTL during the course of growth and development. In a real example with rice, our model has successfully detected several QTL that produce differences in their genetic expression between two contrasting environments. These detected QTL cause significant genotype x environment interactions for some fundamental aspects of growth trajectories. The model provides the basis for deciphering the genetic architecture of trait expression adjusted to different biotic and abiotic environments and genetic relationships for growth rates and the timing of life-history events for any organism. 相似文献
15.
Power and precision of alternate methods for linkage disequilibrium mapping of quantitative trait loci 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Linkage disequilibrium (LD) analysis in outbred populations uses historical recombinations to detect and fine map quantitative trait loci (QTL). Our objective was to evaluate the effect of various factors on power and precision of QTL detection and to compare LD mapping methods on the basis of regression and identity by descent (IBD) in populations of limited effective population size (N(e)). An 11-cM region with 6-38 segregating single-nucleotide polymorphisms (SNPs) and a central QTL was simulated. After 100 generations of random mating with N(e) of 50, 100, or 200, SNP genotypes and phenotypes were generated on 200, 500, or 1000 individuals with the QTL explaining 2 or 5% of phenotypic variance. To detect and map the QTL, phenotypes were regressed on genotypes or (assumed known) haplotypes, in comparison with the IBD method. Power and precision to detect QTL increased with sample size, marker density, and QTL effect. Power decreased with N(e), but precision was affected little by N(e). Single-marker regression had similar or greater power and precision than other regression models, and was comparable to the IBD method. Thus, for rapid initial screening of samples of adequate size in populations in which drift is the primary force that has created LD, QTL can be detected and mapped by regression on SNP genotypes without recovering haplotypes. 相似文献
16.
Pégolo NT Oliveira HN Albuquerque LG Bezerra LA Lôbo RB 《Genetics and molecular biology》2009,32(2):281-287
Genotype by environment interactions (GEI) have attracted increasing attention in tropical breeding programs because of the variety of production systems involved. In this work, we assessed GEI in 450-day adjusted weight (W450) Nelore cattle from 366 Brazilian herds by comparing traditional univariate single-environment model analysis (UM) and random regression first order reaction norm models for six environmental variables: standard deviations of herd-year (RRMw) and herd-year-season-management (RRMw-m) groups for mean W450, standard deviations of herd-year (RRMg) and herd-year-season-management (RRMg-m) groups adjusted for 365-450 days weight gain (G450) averages, and two iterative algorithms using herd-year-season-management group solution estimates from a first RRMw-m and RRMg-m analysis (RRMITw-m and RRMITg-m, respectively). The RRM results showed similar tendencies in the variance components and heritability estimates along environmental gradient. Some of the variation among RRM estimates may have been related to the precision of the predictor and to correlations between environmental variables and the likely components of the weight trait. GEI, which was assessed by estimating the genetic correlation surfaces, had values < 0.5 between extreme environments in all models. Regression analyses showed that the correlation between the expected progeny differences for UM and the corresponding differences estimated by RRM was higher in intermediate and favorable environments than in unfavorable environments (p < 0.0001). 相似文献
17.
利用双单倍体群体剖析水稻产量及其相关性状的遗传基础 总被引:23,自引:0,他引:23
主效QTL、上位性效应和它们与环境的互作(QE)都是数量性状的重要遗传因素。利用籼粳交珍汕97/武育粳2号F1植株上的花药进行组织培养得到的190个双单倍体群体和179个微卫星标记,通过两年两重复田间试验,采用混合线性模型方法分析了9个控制水稻产量及其相关性状的遗传效应,得到57个主效QTL,41对上位性互作,8对QTL与环境的互作和7对上位性效应与环境的互作。单个主效QTL解释这些性状1.3%~25.8%的表型方差。各性状QTL的累积表型贡献率达11.5%~66.8%。大多数性状之间具有显著的表型相关性,相关性较高的性状之间常具有较多共同或紧密连锁的QTL。结果表明,基因的多效性或紧密连锁可能是性状相关的重要遗传基础。 相似文献
18.
Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations 总被引:8,自引:0,他引:8
Li H Ribaut JM Li Z Wang J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(2):243-260
It has long been recognized that epistasis or interactions between non-allelic genes plays an important role in the genetic
control and evolution of quantitative traits. However, the detection of epistasis and estimation of epistatic effects are
difficult due to the complexity of epistatic patterns, insufficient sample size of mapping populations and lack of efficient
statistical methods. Under the assumption of additivity of QTL effects on the phenotype of a trait in interest, the additive
effect of a QTL can be completely absorbed by the flanking marker variables, and the epistatic effect between two QTL can
be completely absorbed by the four marker-pair multiplication variables between the two pairs of flanking markers. Based on
this property, we proposed an inclusive composite interval mapping (ICIM) by simultaneously considering marker variables and
marker-pair multiplications in a linear model. Stepwise regression was applied to identify the most significant markers and
marker-pair multiplications. Then a two-dimensional scanning (or interval mapping) was conducted to identify QTL with significant
digenic epistasis using adjusted phenotypic values based on the best multiple regression model. The adjusted values retain
the information of QTL on the two current mapping intervals but exclude the influence of QTL on other intervals and chromosomes.
Epistatic QTL can be identified by ICIM, no matter whether the two interacting QTL have any additive effects. Simulated populations
and one barley doubled haploids (DH) population were used to demonstrate the efficiency of ICIM in mapping both additive QTL
and digenic interactions. 相似文献
19.
20.
We have identified, using composite interval mapping, quantitative trait loci (QTL) affecting a variety of life history traits (LHTs) in the nematode Caenorhabditis elegans. Using recombinant inbred strains assayed on the surface of agar plates, we found QTL for survival, early fertility, age of onset of sexual maturity, and population growth rate. There was no overall correlation between survival on solid media and previous measures of survival in liquid media. Of the four survival QTL found in these two environments, two have genotype-environment interactions (GEIs). Epistatic interactions between markers were detected for four traits. A multiple regression approach was used to determine which single markers and epistatic interactions best explained the phenotypic variance for each trait. The amount of phenotypic variance accounted for by genetic effects ranged from 13% (for internal hatching) to 46% (for population growth). Epistatic effects accounted for 9-11% of the phenotypic variance for three traits. Two regions containing QTL that affected more than one fertility-related trait were found. This study serves as an example of the power of QTL mapping for dissecting the genetic architecture of a suite of LHTs and indicates the potential importance of environment and GEIs in the evolution of this architecture. 相似文献