共查询到20条相似文献,搜索用时 15 毫秒
1.
Grazyna?Kwapiszewska Markus?Meyer Ralf?Bogumil Rainer?M?Bohle Werner?Seeger Norbert?Weissmann Ludger?Fink
Background
Laser microdissection allows precise isolation of specific cell types and compartments from complex tissues. To analyse proteins from small cell numbers, we combine laser-microdissection and manipulation (LMM) with mass spectrometry techniques. 相似文献2.
Background
One frequent application of microarray experiments is in the study of monitoring gene activities in a cell during cell cycle or cell division. A new challenge for analyzing the microarray experiments is to identify genes that are statistically significantly periodically expressed during the cell cycle. Such a challenge occurs due to the large number of genes that are simultaneously measured, a moderate to small number of measurements per gene taken at different time points, and high levels of non-normal random noises inherited in the data. 相似文献3.
Background
Self-renewal of the epithelium of the small intestine is a highly regulated process involving cell proliferation and differentiation of stem cells or progenitor cells located at the bottom of the crypt, ending ultimately with extrusion of the terminally differentiated cells at the tip of villus. 相似文献4.
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Justin C Yarrow Zachary E Perlman Nicholas J Westwood Timothy J Mitchison 《BMC biotechnology》2004,4(1):21
Background
Cell migration is a complex phenomenon that requires the coordination of numerous cellular processes. Investigation of cell migration and its underlying biology is of interest to basic scientists and those in search of therapeutics. Current migration assays for screening small molecules, siRNAs, or other perturbations are difficult to perform in parallel at the scale required to screen large libraries. 相似文献6.
Cell membrane array fabrication and assay technology 总被引:1,自引:0,他引:1
Background
Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processes has been considerably more difficult. 相似文献7.
Neil R Smalheiser 《Biology direct》2007,2(1):35-15
Background
Many cell types have been reported to secrete small vesicles called exosomes, that are derived from multivesicular bodies and that can also form from endocytic-like lipid raft domains of the plasma membrane. Secretory exosomes contain a characteristic composition of proteins, and a recent report indicates that mast cell exosomes harbor a variety of mRNAs and microRNAs as well. Exosomes express cell recognition molecules on their surface that facilitate their selective targeting and uptake into recipient cells. 相似文献8.
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Thomas U Greiner Gokul Kesavan Anders Ståhlberg Henrik Semb 《BMC developmental biology》2009,9(1):2-13
Background
Pancreatic islets of Langerhans originate from endocrine progenitors within the pancreatic ductal epithelium. Concomitant with differentiation of these progenitors into hormone-producing cells such cells delaminate, aggregate and migrate away from the ductal epithelium. The cellular and molecular mechanisms regulating islet cell delamination and cell migration are poorly understood. Extensive biochemical and cell biological studies using cultured cells demonstrated that Rac1, a member of the Rho family of small GTPases, acts as a key regulator of cell migration. 相似文献11.
HIV-1 TAR element is processed by Dicer to yield a viral micro-RNA involved in chromatin remodeling of the viral LTR 总被引:1,自引:0,他引:1
Zachary Klase Prachee Kale Rafael Winograd Madhur V Gupta Mohammad Heydarian Reem Berro Timothy McCaffrey Fatah Kashanchi 《BMC molecular biology》2007,8(1):63
Background
RNA interference (RNAi) is a regulatory mechanism conserved in higher eukaryotes. The RNAi pathway generates small interfering RNA (siRNA) or micro RNA (miRNA) from either long double stranded stretches of RNA or RNA hairpins, respectively. The siRNA or miRNA then guides an effector complex to a homologous sequence of mRNA and regulates suppression of gene expression through one of several mechanisms. The suppression of gene expression through these mechanisms serves to regulate endogenous gene expression and protect the cell from foreign nucleic acids. There is growing evidence that many viruses have developed in the context of RNAi and express either a suppressor of RNAi or their own viral miRNA. 相似文献12.
Daniel J Morton Elizabeth J Turman Patrick D Hensley Timothy M VanWagoner Thomas W Seale Paul W Whitby Terrence L Stull 《BMC microbiology》2010,10(1):113
Background
Haemophilus influenzae has an absolute aerobic growth requirement for either heme, or iron in the presence of protoporphyrin IX. Both iron and heme in the mammalian host are strictly limited in their availability to invading microorganisms. Many bacterial species overcome iron limitation in their environment by the synthesis and secretion of small iron binding molecules termed siderophores, which bind iron and deliver it into the bacterial cell via specific siderophore receptor proteins on the bacterial cell surface. There are currently no reports of siderophore production or utilization by H. influenzae. 相似文献13.
Chun Liang Feng Sun Haiming Wang Junfeng Qu Robert M Freeman Jr Lee H Pratt Marie-Michèle Cordonnier-Pratt 《BMC bioinformatics》2006,7(1):115-15
Background
Processing raw DNA sequence data is an especially challenging task for relatively small laboratories and core facilities that produce as many as 5000 or more DNA sequences per week from multiple projects in widely differing species. To meet this challenge, we have developed the flexible, scalable, and automated sequence processing package described here. 相似文献14.
Alessandro P Burlina Katherine B Sims Juan M Politei Gary J Bennett Ralf Baron Claudia Sommer Anette Torvin M?ller Max J Hilz 《BMC neurology》2011,11(1):61
Background
Fabry disease is an inherited metabolic disorder characterized by progressive lysosomal accumulation of lipids in a variety of cell types, including neural cells. Small, unmyelinated nerve fibers are particularly affected and small fiber peripheral neuropathy often clinically manifests at young age. Peripheral pain can be chronic and/or occur as provoked attacks of excruciating pain. Manifestations of dysfunction of small autonomic fibers may include, among others, impaired sweating, gastrointestinal dysmotility, and abnormal pain perception. Patients with Fabry disease often remain undiagnosed until severe complications involving the kidney, heart, peripheral nerves and/or brain have arisen. 相似文献15.
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Background
To study the biological function of miRNAs, and to achieve sustained or conditional gene silencing with siRNAs, systems that allow controlled expression of these small RNAs are desirable. Methods for cell delivery of siRNAs include transient transfection of synthetic siRNAs and expression of siRNAs in the form of short hairpins using constitutive RNA polymerase III promoters. Systems employing constitutive RNA polymerase II promoters have been used to express miRNAs. However, for many experimental systems these methods do not offer sufficient control over expression. 相似文献17.
Axel Mosig Stefan Jäger Chaofeng Wang Sumit Nath Ilker Ersoy Kannap-pan Palaniappan Su-Shing Chen 《Algorithms for molecular biology : AMB》2009,4(1):10-9
Background
With the increasing availability of live cell imaging technology, tracking cells and other moving objects in live cell videos has become a major challenge for bioimage informatics. An inherent problem for most cell tracking algorithms is over- or under-segmentation of cells – many algorithms tend to recognize one cell as several cells or vice versa. 相似文献18.
Background
There is an increasing interest to model biochemical and cell biological networks, as well as to the computational analysis of these models. The development of analysis methodologies and related software is rapid in the field. However, the number of available models is still relatively small and the model sizes remain limited. The lack of kinetic information is usually the limiting factor for the construction of detailed simulation models. 相似文献19.
Debra J Taxman Laura R Livingstone Jinghua Zhang Brian J Conti Heather A Iocca Kristi L Williams John D Lich Jenny P-Y Ting William Reed 《BMC biotechnology》2006,6(1):7-16