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1.
Cytology of infection,development and expression of resistance to Plasmodiophora brassicae in canola
The timing and expression of resistance to four isolates of Plasmodiophora brassicae, collected from research sites where pathotypes 2, 3, 5 and 6 (Williams' system) had been dominant when characterised in 2006, were assessed in four new commercial cultivars of canola (Brassica napus) with resistance to clubroot. Each of the resistant cultivars was highly resistant to all four of the isolates, and there was no difference in their response to infection. Root hair infection occurred at high levels, but pathogen development occurred more slowly than in a susceptible cultivar (control). Secondary infection and development in cortical cells was severely inhibited in each of the resistant cultivars; only a few bi‐nucleated plasmodia were observed at 12 days after inoculation (DAI), and plasmodia were rarely observed at 18 and 24 DAI. In contrast, development in the susceptible cultivar had progressed to resting spores by 24 DAI. A dense ring of accumulated reactive oxygen species (ROS) was observed in the endodermis, pericycle and vascular cambium of non‐inoculated controls and inoculated plants of the resistant cultivars. However, the ROS ring disappeared rapidly in infected plants of the susceptible cultivar. Plasmodia invaded the stele of susceptible roots by preferentially colonising the xylem parenchyma cells. Expansion and enlargement of lignified xylem cells was observed by 35 DAI. The absence of any specific points of ROS accumulation or lignification of epidermal or cortical cells in the resistant cultivars indicates that a hypersensitive response is not the main mechanism of resistance in these lines. The uniform response of these resistant cultivars to the four isolates of P. brassicae indicates that the resistance in each cultivar may be conditioned by a gene(s) from a single source that confers broad resistance, because most sources of resistance to P. brassicae are pathotype specific. 相似文献
2.
The distribution and appearance of Ralstonia solanacearum in the upper hypocotyl tissues of root‐inoculated tomato seedlings of resistant rootstock cultivar LS‐89 (a selection from Hawaii 7998) and susceptible cultivar Ponderosa were compared to clarify the mechanism that limits the movement of the bacterial pathogen in resistant tomato tissues. In stems of wilted Ponderosa plants, bacteria colonized both the primary and the secondary xylem tissues. Bacteria were abundant in vessels, of which the pit membranes were often degenerated. All parenchyma cells adjacent to vessels with bacteria were necrotic and some of them were colonized with bacteria. In stems of LS‐89 plants showing no discernible wilting symptoms, bacteria were observed in the primary xylem tissues but not in the secondary xylem tissues. Necrosis of parenchyma cells adjacent to vessels with bacteria was observed occasionally. The pit membranes were often thicker with high electron density. The inner electron‐dense layer of cell wall of parenchyma cells and vessels was thicker and more conspicuous in xylem tissues of infected LS‐89 than in xylem of infected Ponderosa or mock‐inoculated plants. Electron‐dense materials accumulated in or around pit cavities in parenchyma cells next to vessels with bacteria, and in vessels with bacteria. Many bacterial cells appeared normal in vessels, except for those in contact with the pit membranes. These results indicate that R. solanacearum moves from vessel to vessel in infected tissues through degenerated pit membranes and that restricted movement in xylem tissues was the characteristic feature in LS‐89. The limitation in bacterial movement may be related to the thickening of the pit membranes and/or the accumulations of electron‐dense materials in vessels and parenchyma cells. 相似文献
3.
Growth and anatomical responses of plants during latent and pathogenic infection by fungal pathogens are not well understood. The interactions between soybean (Glycine max) and two types of the pathogen Phialophora gregata were investigated to determine how plants respond during latent and pathogenic infection. Stems of soybean cultivars with different or no genes for resistance to infection by P. gregata were inoculated with wildtype or GFP and RFP-labeled strains of types A or B of P. gregata. Plants were sectioned during latent and pathogenic infection, examined with transmitted light or fluorescent microscopy, and quantitative differences in vessels and qualitative differences in infection were assessed using captured images. During latent infection, the number of vessels was similar in resistant and susceptible plants infected with type A or B compared to the control, and fungal infection was rarely observed in vessels. During pathogenic infection, the resistant cultivars had 20 to 25% more vessels than the uninfected plants, and fungal hyphae were readily observed in the vessels. Furthermore, during the pathogenic phase in a resistant cultivar, P.gregata type A-GFP was limited to outside of the primary xylem, while P.gregata type B-RFP was observed in the primary xylem. The opposite occurred with the susceptible cultivar, where PgA-GFP was observed in the primary xylem and PgB-RFP was limited to the interfascicular region. In summary, soybean cultivars with resistance to BSR produced more vessels and can restrict or exclude P. gregata from the vascular system compared to susceptible cultivars. Structural resistance mechanisms potentially compensate for loss of vessel function and disrupted water movement. 相似文献
4.
Summary Massive infusion of conidia ofVerticillium albo-atrum into the xylem of tomato induces a cell wall coating response in resistant and susceptible near-isolines. In the early stages two types of coating material develop in the xylem vessels. The first, designated type A, is formed in association with xylem parenchyma cells that lack secondary walls; the localized accumulation of type A coating in the in the adjacent intercellular spaces, primary walls (i.e., pit membranes) and vessels occurs in conjunction with localized development of apposition wall layers within the parenchyma cells. Type B coating is initially formed in association with xylem parenchyma cells with secondary walls; the localized accumulation of typeB coating in the adjacent intercellular spaces, primary walls (i.e., pit membranes) and vessels occurs in conjunction with development of protective layers within the parenchyma cells. Most vessels are surrounded by a number of parenchyma cells including both cell types; therefore, in most vessels the coatings are mixed in later stages of development (i.e.,> 48 hours). The formation of both types of coating is stopped by the application of L--aminooxy--phenylpropionate, a specific inhibitor of phenylpropanoid synthesis. Histochemically, type A coating resembles lignin and type B, suberin. The data suggest that the coating response is due, wholly or in part to hypersecretion and/or chemical modification of normal cell wall components, induced by the pathogen. 相似文献
5.
Summary From root exudates of three cultivars of chilli (Capsicum annuum L.) 12 amino acids and 7 sugars were detected. Methionine, d-1- phenylalanine, citrulline and d-xylose were detected only from the root exudates of resdistant cultivars. The root exudates of resistant variety inhibited spore germination of the pathogen (Fusarium oxysporum f. sp.capsici), but that of susceptible variety enhanced spore germiantion of the same. Spore germiantion of antagonistic fungi (Trichderma viride andAspergillus sydowi) was also influenced by the root exudates of resistant and susceptible varieties, but the influence was different.Spore germiantion of a number of rhizosphere fungi was studied and in general root exudate of susceptible cultivar enhanced spore germiantion of majority of fungi, but spore germination of antagonistic fungi against the pathogen was inhibited. However, root exudate of resistant cultivar stimulated spore germination of antagonistic fungi. 相似文献
6.
It has been suggested that oligogalacturonides (OGAs) released by bacterial pectinases can induce plant defence responses. To test this hypothesis, resistant tomato cultivar LS-89 and susceptible cultivar Ponderosa were inoculated with either wild-type Ralstonia solanacearum strain K60 or a pectinase-deficient triple mutant K60-509, which lacks endo-polygalacturonase PehA, exo-poly-alpha- d -galacturonosidase PehB, and pectin methylesterase Pme. K60 induced structural defence responses, including electron-dense materials (EDMs) in vessels and apposition layers (ALs) in parenchyma cells adjacent to xylem vessels colonized by bacteria in LS-89 stems. In contrast, LS-89 infected with K60-509 did not have any EDMs in vessels at 4 days after inoculation (DAI), and had them only rarely at 7 DAI. In LS-89 infected with K60-509, ALs were rarely observed in parenchyma cells adjacent to vessels at 4 DAI, and while they were present at 7 DAI, they were thinner than ALs induced by K60. The bacterial density in LS-89 stems infected with K60-509 was lower than in stems infected with K60 at 4 DAI, but the strains reached similar population sizes by 7 DAI, showing the pectinase-deficient mutant colonized resistant stems more slowly than did the wild-type strain. Vessels infected with K60-509 contained fewer EDMs at 7 DAI than were observed at either 4 or 7 DAI in vessels colonized by K60, although bacterial density in the xylem tissues containing K60-509 at 7 DAI was about the same as in the xylem tissues containing K60 at 4 DAI. Neither the wild-type strain nor the pectinase-deficient mutant induced these histopathological changes on susceptible cultivar Ponderosa. These results indicate that R. solanacearum pectinases play some role in eliciting histopathological changes in LS-89, likely by releasing OGAs that trigger plant structural defences. 相似文献
7.
Pathogen‐induced conditioning of the primary xylem vessels – a prerequisite for the formation of bacterial emboli by Pectobacterium atrosepticum
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V. Y. Gorshkov A. G. Daminova P. V. Mikshina O. E. Petrova M. V. Ageeva V. V. Salnikov T. A. Gorshkova Y. V. Gogolev 《Plant biology (Stuttgart, Germany)》2016,18(4):609-617
Representatives of Pectobacterium genus are some of the most harmful phytopathogens in the world. In the present study, we have elucidated novel aspects of plant–Pectobacterium atrosepticum interactions. This bacterium was recently demonstrated to form specific ‘multicellular’ structures – bacterial emboli in the xylem vessels of infected plants. In our work, we showed that the process of formation of these structures includes the pathogen‐induced reactions of the plant. The colonisation of the plant by P. atrosepticum is coupled with the release of a pectic polysaccharide, rhamnogalacturonan I, into the vessel lumen from the plant cell wall. This polysaccharide gives rise to a gel that serves as a matrix for bacterial emboli. P. atrosepticum‐caused infection involves an increase of reactive oxygen species (ROS) levels in the vessels, creating the conditions for the scission of polysaccharides and modification of plant cell wall composition. Both the release of rhamnogalacturonan I and the increase in ROS precede colonisation of the vessels by bacteria and occur only in the primary xylem vessels, the same as the subsequent formation of bacterial emboli. Since the appearance of rhamnogalacturonan I and increase in ROS levels do not hamper the bacterial cells and form a basis for the assembly of bacterial emboli, these reactions may be regarded as part of the susceptible response of the plant. Bacterial emboli thus represent the products of host–pathogen integration, since the formation of these structures requires the action of both partners. 相似文献
8.
Graskova I. A. Romanenko A. S. Vladimirova S. V. Kolesnichenko A. V. 《Russian Journal of Plant Physiology》2004,51(4):476-479
The effects of the ring rot causal agent Clavibacter michiganensis subsp. sepedonicus (a virulent strain 5369) on the peroxidase activity of various tissues of potato plants grown under axenic conditions were studied. Root infection enhanced peroxidase activity in all plant tissues (roots, leaves, and stems). In the resistant cv. Lugovskoi, peroxidase activity was much higher than in the susceptible cv. Luk'yanovskii. Co-culturing of the suspension cells of these potato cultivars with the bacterial pathogen also activated peroxidase in the cells of the resistant cultivar; in the cells of the susceptible cultivar, peroxidase activation was less pronounced. Treating suspension cell with exopolysaccharides secreted by the pathogen enhanced the activity of extra- and intracellular peroxidases, and the degree of this enhancement differed in the two potato cultivars. 相似文献
9.
Romanenko A. S. Lomovatskaya L. A. Graskova I. A. 《Russian Journal of Plant Physiology》2002,49(5):690-695
Test-tube plants and suspension cell cultures of two cultivars of the potato (Solanum tuberosum L.) differing in their resistance to ring rot caused by Clavibacter michiganensis subsp. sepedonicus and six strains of this bacterium were used to test the relationship between the virulence, the leaf ability to adsorb bacteria, and the symptoms of the disease. In addition to chlorosis and drying, heavy inoculation with virulent strains caused unusual symptoms, such as leaf necrotic lesions. In the resistant cultivar, the necrotic lesions were predominantly local, whereas in the susceptible cultivar, they expanded. Unlike the susceptible cultivar, suspension cells of the resistant cultivar weakly adhered bacteria of the tested strains. Bacteria entered the plants through the leaf stomata. The sorption and penetration were much more pronounced in the susceptible cultivar. It was concluded that strain virulence varies depending on the conditions of inoculation, and uncharacteristic symptoms (necrotic lesions) arise. The local necrotic lesions are considered a hypersensitive response, and exopolysaccharides of the pathogen as the factors of virulence. 相似文献
10.
CROWDY SH 《The Annals of applied biology》1949,36(4):483-495
The development of cankers caused by Nectria galligena on apple is described; the pathogen exploits all the tissues outside the xylem and will also penetrate the xylem to an appreciable depth, invading the xylem parenchyma, vessels and fibres.
Spread in the peripheral tissues is checked to some extent by successive barriers laid down by wound phellogens, but the barriers are eventually passed by the mycelium. There appear to be two distinct ways in which these barriers are passed: there may be direct penetration which appears to be associated with an aggregation of the mycelium into blocks, and symptoms suggestive of mechanical rupture; or there is an alternative route in which the pathogen grows in the lumen of the fibres and emerges behind the barrier.
In the xylem the spread of the pathogen is checked by tyloses and gumming in the vessels, and by gumming in the parenchymatous tissues, but in the fibres there appears to be no defence mechanism, and the spread beyond the gum barriers was usually recorded in the xylem fibres. It is suggested that the presence of the pathogen in the xylem fibres might provide the explanation of the formation of cankers on partially healed pruning cuts, and this type of canker is described in some detail.
The development of the canker depends on the balance between the development of the pathogen and the resistance of the host, and there is some discussion on the relation of this balance to the control of the disease. 相似文献
Spread in the peripheral tissues is checked to some extent by successive barriers laid down by wound phellogens, but the barriers are eventually passed by the mycelium. There appear to be two distinct ways in which these barriers are passed: there may be direct penetration which appears to be associated with an aggregation of the mycelium into blocks, and symptoms suggestive of mechanical rupture; or there is an alternative route in which the pathogen grows in the lumen of the fibres and emerges behind the barrier.
In the xylem the spread of the pathogen is checked by tyloses and gumming in the vessels, and by gumming in the parenchymatous tissues, but in the fibres there appears to be no defence mechanism, and the spread beyond the gum barriers was usually recorded in the xylem fibres. It is suggested that the presence of the pathogen in the xylem fibres might provide the explanation of the formation of cankers on partially healed pruning cuts, and this type of canker is described in some detail.
The development of the canker depends on the balance between the development of the pathogen and the resistance of the host, and there is some discussion on the relation of this balance to the control of the disease. 相似文献
11.
Hypersensitive response, cell death and release of hydrogen peroxide as measures of host and non‐host defense mechanisms upon inoculation with the downy mildew pathogen Sclerospora graminicola were studied histochemically at the light microscopy level. The materials consisted of coleoptile tissues of the highly susceptible (cv. HB3), highly resistant (cv. IP18293) and induced resistant pearl millet host seedlings and non‐host sorghum (cv. SGMN10/8) and cotyledon of french bean (cv. S9). Resistance up to 80% protection against the downy mildew pathogen was induced in the highly susceptible HB3 cultivar of pearl millet by treating the seeds with 2% aqueous leaf extract of Datura metel for 3 h. Time course study with the pathogen inoculated highly resistant pearl millet cultivar revealed the appearance of hypersensitive response in 20% of seedlings as necrotic spots as early as 2 h after inoculation. In contrast, a similar reaction was observed in the highly susceptible pearl millet cultivar only 8 h after inoculation with the pathogen. In induced resistant seedlings, appearance of hypersensitive response was recorded 4 h after inoculation. Delayed hypersensitive response was observed in both the non‐host species at 10 h after inoculation. Hypersensitive response in the seedlings of the highly resistant pearl millet cultivar 24 h after inoculation showed 100% hypersensitive response, which was not observed in susceptible and non‐host species, although the induced resistant seedlings showed 90% hypersensitive response after that period of time. Cell death in the tissues of the test seedlings was also observed to change with time. Statistical analysis revealed that the tissues of highly resistant pearl millet seedlings required 2.9 h to attain 50% cell death. Tissues of induced resistant and highly susceptible pearl millet seedlings required 4.65 and 6.50 h respectively. In non‐hosts, 50% cell death was not recorded. Quantification of hydrogen peroxide in the tissue periplasmic spaces of the test seedlings revealed 2.94 h as the time required for 50% hydrogen peroxide accumulation in the tissues of highly resistant pearl millet seedlings. Tissues of induced resistant and highly susceptible pearl millet seedlings needed 3.76 and 5.5 h respectively. Fifty percent hydrogen peroxide localisation in non‐hosts could not be recorded. These results suggested the involvement of hydrogen peroxide, cell death and hypersensitive response in pearl millet host defense against S. graminicola. 相似文献
12.
During the September‐December season of 1990, severe symptoms of Fusarium wilt were for the first time observed on a popular climbing bean (Phaseolus vulgaris L.) cultivar. G 2333. introduced within the previous 5 years. Seventy‐three bean genotypes were screened for resistance lo the disease, using artificial inoculation. The effect of inoculation density on the reaction of four selected genotypes was also investigated. Of the 29 climbing bean genotypes evaluated, 19 were resistant, including 11 of the 15 pre‐release or released cultivars. Of the 44 bush bean cultivars evaluated, 28 were resistant, five were intermediate and 11 were susceptible. All susceptible cultivars showed vascular discoloration. In both susceptible and resistant genotypes, the fungus spread almost equally from the entry points in inoculated roots to the base of the plants, but colonization and vertical spread within the vascular system were markedly less in resistant than in susceptible cultivars. At 20 and 30 cm above soil level, the fungus was only recovered from susceptible cultivars. Increasing inoculum density from 102 to 107 conidia/ml did not affect the resistance of cultivars RWR 950 and G 685 but. in the susceptible cultivars G 2333 and MLB‐48‐89 A. it resulted in early appearance, high incidence and severity of the disease. 相似文献
13.
Vascular defense responses in rice: peroxidase accumulation in xylem parenchyma cells and xylem wall thickening. 总被引:1,自引:0,他引:1
E Hilaire S A Young L H Willard J D McGee T Sweat J M Chittoor J A Guikema J E Leach 《Molecular plant-microbe interactions : MPMI》2001,14(12):1411-1419
The rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae is a vascular pathogen that elicits a defensive response through interaction with metabolically active rice cells. In leaves of 12-day-old rice seedlings, the exposed pit membrane separating the xylem lumen from the associated parenchyma cells allows contact with bacterial cells. During resistant responses, the xylem secondary walls thicken within 48 h and the pit diameter decreases, effectively reducing the area of pit membrane exposed for access by bacteria. In susceptible interactions and mock-inoculated controls, the xylem walls do not thicken within 48 h. Xylem secondary wall thickening is developmental and, in untreated 65-day-old rice plants, the size of the pit also is reduced. Activity and accumulation of a secreted cationic peroxidase, PO-C1, were previously shown to increase in xylem vessel walls and lumen. Peptide-specific antibodies and immunogold-labeling were used to demonstrate that PO-C1 is produced in the xylem parenchyma and secreted to the xylem lumen and walls. The timing of the accumulation is consistent with vessel secondary wall thickening. The PO-C1 gene is distinct but shares a high level of similarity with previously cloned pathogen-induced peroxidases in rice. PO-C1 gene expression was induced as early as 12 h during resistant interactions and peaked between 18 and 24 h after inoculation. Expression during susceptible interactions was lower than that observed in resistant interactions and was undetectable after infiltration with water, after mechanical wounding, or in mature leaves. These data are consistent with a role for vessel secondary wall thickening and peroxidase PO-C1 accumulation in the defense response in rice to X. oryzae pv. oryzae. 相似文献
14.
Pathogenicity test ofFusarium oxysporum on ten cultivars of soybean revealed Soymax and Punjab-1 to be most resistant while JS-2 and UPSM-19 were most susceptible.
Antigens were prepared from the roots of all the ten varieties of soybean and the mycelium ofF. oxysporum. Polyclonal antisera were raised against the mycelial suspension ofF. oxysporum and the root antigen of the susceptible cultivar UPSM-19. Cross reactive antigens shared by the host and the pathogen were
detected first by immunodiffusion. The immunoglobulin fraction of the antiserum was purified by ammonium sulfate precipitation
and DEAE-Sephadex column chromatography. The immunoglobulin fractions were used for detection of cross-reactive antigens by
enzyme-linked immunosorbent assay. In enzyme-linked immunosorbent assay, antigens of susceptible cultivars showed higher absorbance
values when tested against the purified anti-F. oxysporum antiserum. Antiserum produced against UPSM-19 showed cross-reactivity with the antigens of other cultivars. Indirect staining
of antibodies using fluorescein isothiocyanate indicated that in cross-sections of roots of susceptible cultivar (UPSM-19)
cross-reactive antigens were concentrated around xylem elements, endodermis and epidermal cells, while in the resistant variety,
fluorescence was concentrated mainly around epidermal cells and distributed in the cortical tissues. CRAs were also present
in microconidia, macroconidia and chlamydospores of the fungus. 相似文献
15.
Analysis of the distribution of copper amine oxidase in cell walls of legume seedlings 总被引:5,自引:0,他引:5
Maria Laurenzi Alex J. Tipping Susan E. Marcus Paul J. Knox Rodolfo Federico Riccardo Angelini Michael J. McPherson 《Planta》2001,214(1):37-45
Copper-containing amine oxidase (CuAO) has been proposed to play a role in H2O2 production in plant cell walls during cell development and in response to pathogen attack. We have compared the localisation of CuAO in pea (Pisum sativum L.), lentil (Lens culinaris M.) and chick pea (Cicer arietinum L.) grown under different light conditions, using both immuno- and histochemical techniques. The enzyme was detected by indirect immunofluorescence in the cell walls of parenchyma tissues of etiolated pea and lentil plants and was particularly abundant at intercellular spaces. Upon de-etiolation, CuAO largely disappeared from cortical cell walls except in the region of intercellular spaces. In the apical internode of light-grown seedlings, CuAO occurred mainly in cortical cell walls and, to some extent, in cell walls of xylem vessels. In both the elongation zone and mature regions of roots, CuAO was restricted to cortical cell walls and some cell junctions close to the meristem. Extensin epitopes co-localised to intercellular spaces of the cortex in de-etiolated pea, indicating that CuAO may have a role in cell wall strengthening at intercellular spaces. In chick pea, the localisation of the enzyme varied between different cultivars that have differing susceptibility to the fungus Ascochyta rabiei. In a susceptible cultivar Calia, immunogold labelling localised CuAO to cell walls of the cortex, as in lentil and pea, while in a resistant cultivar Sultano, it was most abundant in xylem vessels and, in light-grown plants, in the epidermis. These expression patterns are discussed with regard to the possible functions of amine oxidase in cell growth, cell differentiation and pathogen resistance. 相似文献
16.
Behrouz Ehsani-Moghaddam Marie Thérèse Charles Odile Carisse Shahrokh Khanizadeh 《Archives Of Phytopathology And Plant Protection》2013,46(7):492-500
Abstract Macroscopic symptoms were observed in two strawberry cultivars, with the degree of symptom intensity varying depending on the susceptibility of the cultivars, i.e. resistant or susceptible. The symptoms presented as red spots and were observed 30 d following leaf tissue inoculation with the Mycosphaerella fragariae pathogen. A comparison of the superoxide dismutase isoform profiles obtained by gel electrophoresis in all samples extracted from both resistant and susceptible cultivars indicated one constant sharp band, identified as Mn[sbnd]SOD with a molecular mass of 19 kDa. The intensity of this band was higher in all samples derived from the resistant cultivar than in those from the susceptible cultivar. Another superoxide dismutase (SOD) isoform, identified as CuZn[sbnd]SOD with a molecular mass of 16 kDa, was detected in all soluble proteins derived from the resistant cultivar. This isoform was not observed in the susceptible cultivar; however, following an incremental increase in the amount of loaded protein, it was illuminated as a faint band in a sample collected 3 d after inoculation, indicating insufficient production of the CuZn[sbnd]SOD isoform in the susceptible cultivar during an oxidative burst induced by the M. fragaria pathogen. Several bands were also characterized in both cultivars containing Fe and Mn as their co-factors (Fe, Mn[sbnd]SOD). Unlike in the resistant cultivar, where the activity of Fe, Mn[sbnd]SOD isoforms gradually and regularly increased and reached its highest level on the third day after inoculation, the activity of the isoforms changed irregularly over 20 days of study in the susceptible cultivar. 相似文献
17.
Xiangling Fang John Kuo Ming Pei You Patrick M. Finnegan Martin John Barbetti 《Plant and Soil》2012,358(1-2):75-89
Background and aims
Strawberry (Fragaria x ananassa) is a high-value crop worldwide. Fusarium oxysporum f. sp. fragariae causes rapid wilting and death of strawberry plants and severe economic losses worldwide. To date, no studies have been conducted to determine colonisation of either susceptible or resistant strawberry plants by F. oxysporum f. sp. fragariae, or whether plant colonisation by F. oxysporum f. sp. fragariae differs between susceptible and resistant cultivars.Methods
Colonisation of strawberry plants by a pathogenic isolate of F. oxysporum f. sp. fragariae was examined both on the root surface and within root tissue of one resistant cv. Festival and one susceptible cv. Camarosa using light and scanning electron microscopy from 4?h to 7?d post inoculation (pi).Results
Resistant cv. Festival significantly impeded the spore germination and penetration from 4 to 12 hpi and subsequent growth and colonisation by this pathogen until 7 dpi compared with susceptible cv. Camarosa. At 7 dpi, fungal colonisation in resistant cv. Festival remained mainly confined to the epidermal layer of the root, while in susceptible cv. Camarosa, hyphae not only had heavily colonised the cortical tissue throughout but had also colonised vascular tissues.Conclusions
This study demonstrates for the first time that resistance of a strawberry cultivar to F. oxysporum f. sp. fragariae is a result of impedance of pathogen growth and colonisation both on the plant surface and within host tissues. Resistance mechanisms identified in this study will be of high value for breeding programmes in developing new disease-resistant cultivars to manage this serious strawberry disorder. 相似文献18.
19.
【背景】寄生帚梗柱孢霉是花生黑腐病的病原菌,被我国列为重要的进境植物检疫性有害生物。该病菌2009年已入侵我国广东,造成花生植株基部腐烂而死亡,严重威胁花生生产安全。筛选与种植抗病品种是防控该病害的重要措施。【方法】收集广东推广种植的15个主要花生品种,通过人工接种方法,鉴定这些品种对花生黑腐病菌的抗性水平。【结果】15个供试花生品种中,湛红2号、湛油62等2个品种表现为抗病;湛油75、湛油82、粤油390、粤油410、仲恺花44、仲恺花99、汕油诱1号等7个品种表现为中抗;花育33号、汕油523、汕油辐1号、粤油18、湛油53等5个品种表现为感病;仲恺花332表现为高感。【结论与意义】目前广东生产上推广种植的花生品种多数对黑腐病菌表现为抗病或中抗水平,部分品种表现为感病或高感。该结果可为我省花生品种的推广与布局提供依据。 相似文献
20.
A. S. Romanenko L. A. Lomovatskaya T. N. Shafikova G. B. Borovskii N. V. Krivolapova 《Journal of Phytopathology》2003,151(1):1-6
The interaction of extracellular polysaccharides (EPS) of the potato ring rot bacterial pathogen Clavibacter michiganensis ssp. sepedonicus (Spieck. et Kott.) Skaptason et Burkh. (Cms) with protoplasts isolated both from leaf cells of plants grown in vitro and microsomal membrane fractions obtained from cell suspension cultures of two potato (Solanum tuberosum L.) cultivars contrasted by their resistance to this pathogen was studied. The EPS intensively bind to protoplast surfaces and microsomal membranes of the susceptible cultivar but not to those of the resistant cultivar. Treatment with protease, excess of unlabelled EPS, and with dextran, did not lead to the binding of fluorochrome‐labelled EPS to protoplasts and microsomal membranes (from both cultivars). It is proposed that (a) a great number of receptors to EPS Cms are present in the plasma membranes of potato cells of susceptible cultivars, (b) these receptors contain proteinaceous sites exposed on the external side of the plasma membrane which participate in EPS binding, and (c) the plasma membranes of cells of resistant cultivars contain a small but sufficient quantity of receptors to EPS able to induce defensive responses in plants. 相似文献