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1.
The relative toxicity of someacaricides to the predatory mite, Phytoseiulus persimilis and the twospottedspider mite, Tetranychus urticae (Acari: Phytoseiidae, Tetranychidae) wasevaluated in laboratory. Five of theacaricides tested, including bifenazate,acequinocyl, chlorfenapyr, flufenoxuron andfenbutatin oxide, were much less toxic to adultfemales and immatures of P. persimilisthan to those of T. urticae, and adultfemale predators treated with these fiveacaricides produced 84±96% as many eggs as didcontrol females. Etoxazole did not seriouslyaffect the survival and reproduction of adultfemale predators but caused high mortalityrates in eggs and larvae of P.persimilis. Milbemectin and fenazaquin werevery toxic to adult females and immatures ofP. persimilis. Adult female predatorssurvived on a diet of spider mites treated withbifenazate, acequinocyl, chlorfenapyr,flufenoxuron and fenbutatin oxide, and theirfecundity, prey consumption and the sex ratioof the progeny were not substantially affected. Based on the results, bifenazate, acequinocyl,chlorfenapyr, flufenoxuron and fenbutatin oxideappeared to be the promising candidates for usein integrated mite management programs whereP. persimilis is the major naturalenemy.  相似文献   

2.
The effect of either untreated or treated adults of the spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) by Beauveria bassiana (Bals.) Vuillemin (Ascomycota: Hypocreales) DEBI008 at 1×106 (conidia/ml) was investigated on developmental stages and life table parameters of Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae) under laboratory conditions. Four time intervals (0, 24, 48 and 72 h post-inoculation of spider mites) were considered for studying the predator characteristics as different treatments. Duration of each life stage, longevity, reproduction rate, intrinsic rate of natural increase (r m ), net reproductive rate (R 0), mean generation time (T) and finite rate of increase (λ) of the P. persimilis were calculated on both untreated and B. bassiana treated spider mite adults. Data analysis showed that longevity and fecundity of predatory mites fed on untreated and treated mites (time interval 0) were higher in comparison with other time intervals after inoculation. The entomopathogenic fungus adversely affected longevity and fecundity of the predatory mite. Fertility life table parameters of predatory mites fed on T. urticae treated by B. bassiana at different time intervals showed that T, R 0, λ and r m are strongly affected by the fungus presence and these parameters had significant differences among time treatments. The least r m value was observed in the time interval of 72 h post-inoculation. The fitness of T. urticae was affected by B. bassiana 24, 48 and 72 h post-inoculation of mite adults, and consequently it caused decreased longevity of P. persimilis and accordingly a decrease in the intrinsic rate of natural increase of the predator.  相似文献   

3.
The effectiveness of the predatory mite Phytoseiulus persimilis Athias‐Henriot (Acari: Phytoseidae) in controlling the two‐spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) was evaluated in commercial strawberry greenhouses in two areas of Korea. Optimum release times for P. persimilis were investigated by case studies. Control effects of the two‐spotted spider mite by release of the predatory mite were shown. There was mostly good control by the release of P. persimilis when there were fewer than 0.9 T. urticae per leaf. We recommend it would be ideal to release P. persimilis twice in December and once in February for very early strawberry harvest, and three times from February to March (after overwintering) for early harvest.  相似文献   

4.
From 1995 to 1999, four experiments were conducted on greenhouse roses to assess the effectiveness of the nC24 petroleum spray oil (PSO), D-C-Tron Plus, against two-spotted mite, Tetranychus urticae Koch (Acarina: Tetranychidae), and to determine how the oil could be most efficiently and effectively used in combination with the predatory mite Phytoseiulus persimilis Athias-Henriot (Acarina: Phytoseiidae) in an integrated pest management program. The results showed that 0.5% PSO applied fortnightly to roses gave excellent protection from T. urticae infestation when the mite population was not already established. However, PSO applied after roses were infested with T. urticae above the economic threshold only stabilised populations without reducing them below that threshold. Populations of P. persimilis in the upper and lower canopies were unchanged after two sprays of PSO at 7-day intervals, and application of PSO to the upper canopy was as effective in controlling T. urticae in the presence of P. persimilis as spraying the entire plant. Combining PSO with P. persimilis gave better control of T. urticae than using P. persimilis alone. The most cost-effective use of PSO in the presence of P. persimilis is, therefore, to apply spray only to the upper canopy. This will not affect control of powdery mildew with PSO. Comparison of a control program for T. urticae based on the monitored use of synthetic miticides with that based on calendar application of PSO revealed that both gave equally effective control. The benefits of combining PSO and P. persimilis in an integrated pest management program for T. urticae on roses over a program based on synthetic fungicides are discussed.  相似文献   

5.

Background

The two-spotted spider mite, Tetranychus urticae, is infected with Wolbachia, which have the ability to manipulate host reproduction and fitness. MicroRNAs (miRNAs) are small non-coding RNAs that are involved in many biological processes such as development, reproduction and host-pathogen interactions. Although miRNA was observed to involve in Wolbachia-host interactions in the other insect systems, its roles have not been fully deciphered in the two-spotted spider mite.

Results

Small RNA libraries of infected and uninfected T. urticae for both sexes (in total four libraries) were constructed. By integrating the mRNA data originated from the same samples, the target genes of the differentially expressed miRNAs were predicted. Then, GO and pathway analyses were performed for the target genes. Comparison of libraries showed that Wolbachia infection significantly regulated 91 miRNAs in females and 20 miRNAs in males, with an overall suppression of miRNAs in Wolbachia-infected libraries. A comparison of the miRNA and mRNA data predicted that the differentially expressed miRNAs negatively regulated 90 mRNAs in females and 9 mRNAs in males. An analysis of target genes showed that Wolbachia-responsive miRNAs regulated genes with function in sphingolipid metabolism, lysosome function, apoptosis and lipid transporting in both sexes, as well as reproduction in females.

Conclusion

Comparisons of the miRNA and mRNA data can help to identify miRNAs and miRNA target genes involving in Wolbachia-host interactions. The molecular targets identified in this study should be useful in further functional studies.

Electronic supplementary material

The online version of this article (doi:10.1186/1471-2164-15-1122) contains supplementary material, which is available to authorized users.  相似文献   

6.
The carmine spider mite, Tetranychus cinnabarinus (Boisduval) and the twospotted spider mite, Tetranychus urticae Koch, are serious pests of strawberries and many other horticultural crops. Control of these pests has been heavily dependent upon chemical acaricides. Objectives of this study were to determine the resistance status of these two pest species to commonly used acaricides on strawberries in a year‐round intensive horticultural production region. LC90 of abamectin for adult carmine spider mites was 4% whereas that for adult twospotted spider mites was 24% of the top label rate. LC90s of spiromesifen, etoxazole, hexythiazox and bifenazate were 0.5%, 0.5%, 1.4% and 83% of their respective highest label rates for carmine spider mite eggs, 0.7%, 2.7%, 12.1% and 347% of their respective highest label rates for the nymphs. LC90s of spiromesifen, etoxazole, hexythiazox and bifenazate were 4.6%, 11.1%, 310% and 62% of their respective highest label rates for twospotted spider mite eggs, 3%, 13%, 432,214% and 15% of their respective highest label rates for the nymphs. Our results suggest that T. cinnabarinus have developed resistance to bifenazate and that the T. urticae have developed resistance to hexythiazox. These results strongly emphasize the need to develop resistance management strategies in the region.  相似文献   

7.
Tetranychus urticae distribution on spatial scale both within leaf and within plant was assessed by the index of dispersion (Id), Lloyd's mean crowding index (Imc), Lloyd's patchiness index (Ip) and Morisita's index (I δ). Id values in all leaves and leaf parts promised aggregated distribution regardless of leaf position and leaf areas. The values of Imc estimated were all larger than the mean. The value of Ip and I δ  also indicated increased degree of aggregation and clumping in all leaves and leaf areas. While pattern of distribution is same in all the leaves and leaf parts, population estimated is high in young fully opened top leaves than the grown-up middle and bottom leaves. Within the young leaf, top area of upper side of the leaf housed relatively increased number of T. urticae than bottom area of upper side of the leaf. Because young fully opened leaves consistently contained major proportion of mites (48.33%), particularly the top leaf area (79.62%), these leaves and leaf areas can be used as sampling unit for population estimation to minimise the time spent on sampling. However, it warrants future research for predictive models to associate a number of mites of top leaf to other leaves and top area to other areas. Further, releasing predators in young fully opened leaves may increase predator efficiency if supplementary studies on predator–prey relationship on spatial scale are triggered.  相似文献   

8.
Primary sex ratio of arrhenotokous tetranychid mites depends on the age-specific schedule of ratios of progeny produced by individual females. Since primary sex ratio is conditional upon female age and since the proportion of females of a given age in a population is influenced by the rate of increase, both the agespecific ratio of males to females and the overall ratio of males to females depends on the rate of increase. We given an expression for this dependence and compare it to experimental results for the two-spotted spiter mite. These results imply that there is no standard sex ratio of spider mites because of interpopulation variation in rates of increase.
Résumé Le taux sexuel des acariens tétranychidés arrhénotoques est défini comme le rapport de la somme des nombres de mâles sur la somme des nombres de femelles pour chaque âge de la distribution en âges. Comme le taux sexuel primaire dépend de l'âge de la femelle et comme la proportion de femelles d'un âge donné dans la population est influencée par le taux d'accroissement, alors le rapport mâle/femelle spécifique d'un âge déterminé et le rapport global mâle/femelle dépendent du taux d'accroissement. Nous avons cherché à exprimer cette dépendance et à la comparer aux résultats expérimentaux pour Tetranychus urticae. Ces résultats signifient qu'il n'y a pas de taux sexuel caractéristique de Tétranyques par suite des variations du taux d'accroissement suivant les populations.
  相似文献   

9.
Choosing a suitable habitat is a main step in the settlement process, particularly for species having weak movement abilities. Reliable cues are thus needed for habitat selection. In silk-spinning arthropods, silk can be used as a social cue to select an appropriate location. Silk can also provide information on the presence of related or non-related individuals. In this article, we compare the settlement behaviour of two strains of the two-spotted spider mite, Tetranychus urticae, in response to the presence of silk woven by an individual of its own or another strain. We then examined how individuals behaved when confronted with both types of silk (own/another strain) simultaneously. Both strains were sensitive to related silk. Settlement decision for both strains did not differ according to the origin of the silk. Mites used the silk as a communication cue for habitat selection and strain discrimination. Our results provide experimental evidence for the use of multiple social cues in the settlement decision by weaving mites.  相似文献   

10.
智利小植绥螨密度对朱砂叶螨产卵能力的影响   总被引:1,自引:0,他引:1       下载免费PDF全文
智利小植绥螨是朱砂叶螨的专性捕食者。本研究在室内,研究了智利小植绥螨密度对朱砂叶螨存活及生殖的影响。智利小植绥螨对朱砂叶螨存活干扰结果表明:干扰后24 h各处理朱砂叶螨的死亡率均显著高于对照,且死亡率随干扰密度增大而提高,10头捕食螨/叶碟处理高达76.67%,为对照处理的14.38倍。但是干扰48 h后对叶螨存活影响不大。对朱砂叶螨产卵干扰结果表明:干扰后各时间段朱砂叶螨单雌产卵量均随智利小植绥螨干扰密度的增加而降低。干扰24 h后,除1头捕食螨/叶碟处理与对照差异不显著外,其他各处理均与对照有显著差异。干扰48 h后,10头捕食螨/叶碟处理的叶螨单头产卵量是2.80粒,仅为对照的28.5%。但是干扰96h后,除10头捕食螨/叶碟处理与对照差异显著外,其余各处理与对照均差异不显著。本研究还分析了干扰对96 h内朱砂叶螨单雌总产卵量影响,处理5头/叶碟和10头/叶碟均显著低于对照,并且10头/叶碟显著低于5头/叶碟;1头/叶碟和3头/叶碟与对照相当,但是均显著高于10头/叶碟。结果显示,智利小植绥螨密度对朱砂叶螨有较强的生殖干扰作用,并且捕食螨密度越大,干扰作用越强,后代增殖潜能越小。  相似文献   

11.
ABSTRACT. The response of the predatory mite, Phytoseiulus persimilis Athias-Henriot, to steep gradients of a volatile kairomone emitted by its prey, Tetranychus urticue Koch, was studied in a vertical air flow chamber. The orientation to wind direction was eliminated by using an olfactometer that had an air stream approaching the predator from below a gauze screen upon which the predator walked. The steep gradient of odour was obtained by putting a cylinder filled with prey-infested leaves vertically below the screen. Starved predators were arrested in the odour patch by walking more slowly and tortuously than well-fed predators. The latter mites did not show a significant ortho- or klinokinetic response to the presence of odour. Both well-fed and starved predators showed a chemotactic response to steep gradients at the border of the circular odour patch. Predators that happened to walk out of the patch, frequently turned back to it. This response is presumably based on idiothetic information about the predator's immediately previous walking directions, because it occurred in the odour-free zone after passing the steep gradient of prey odour. Right-about turns can help the predator to stay in static odour plumes with steep gradients at the borders. This type of plume is present only close to the odour source. Further away from the source the odour plume tends to move to and fro due to variation in wind direction. For the predator to keep track of these snaking plumes the right-about turns are unlikely to be of any value because the response is of short duration and because the response to a moving plume appeared to be inadequate; by moving the cylinder below the screen (and consequently the odour patch) it was found that the predator turned back even if the odour gradient was made to pass the predator in the same direction as that of the predator's movement.  相似文献   

12.
We studied the role of methyl salicylate as an important part of volatile blends in searching behaviour of the predatory mite, Phytoseiulus persimilis (Athias-Henriot). We showed that Methyl Salicylate (MeSa) attracted the predatory mites (1 h starved) in a dose range of 0.02–20?μg. The highest attraction was recorded at the dose of 0.2?μg. The dose of 0.002?μg was considered under the detection level of the predator. P. persimilis could not detect the extra amount of MeSa (100 and 200?μg). The probable effect of starvation period on the predator response is discussed.  相似文献   

13.
This study characterizes the timing of feeding, moving and resting for the two-spotted spider mite, Tetranychus urticae Koch and a phytoseiid predator, Phytoseiulus persimilis Athias-Henriot. Feeding is the interaction between T. urticae and plants, and between P. persimilis and T. urticae. Movement plays a key role in locating new food resources. Both activities are closely related to survival and reproduction. We measured the time allocated to these behaviours at four ages of the spider mite (juveniles, adult females immediately after moult and adult females 1 and 3 days after moult) and two ages of the predatory mite (juveniles and adult females). We also examined the effect of previous spider mite-inflicted leaf damage on the spider mite behaviour. Juveniles of both the spider mite and the predatory mite moved around less than their adult counterparts. Newly emerged adult female spider mites spent most of their time moving, stopping only to feed. This represents the teneral phase, during which adult female spider mites are most likely to disperse. With the exception of this age group, spider mites moved more and fed less on previously damaged than on clean leaves. Because of this, the spider mite behaviour was initially more variable on damaged leaves. Phytoseiulus persimilis rested at all stages for a much larger percentage of the time and spent less time feeding than did T. urticae; the predators invariably rested in close proximity to the prey. Compared to adult predators, juveniles spent approximately four times as long handling a prey egg. The predator-prey interaction is dependent upon the local movement of both the predators and prey. These details of individual behaviours in a multispecies environment can provide an understanding of population dynamics.  相似文献   

14.
Abstract

The acaricidal potential of fourteen essential oils and fourteen of their major monoterpenoids were tested against the Two-spotted spider mite Tetranychus urticae. Two different time intervals of 24 and 48 hrs were used in evaluation. The assays after 24 hrs showed that mattercary, fennel, caraway, garlic, cinnamon, chenopodium and eucalyptus were highly potent. As for monoterpenoids, chlorothymol was found to be the most effective against T. urticae followed by thymol, carvacrol and cinnamaldehyde.  相似文献   

15.
Two female Phytoseiulus persimilis and their offspring eliminated two-spotted spider mite Tetranychus urticae from hop leaf discs faster than two female Neoseiulus californicus and their offspring at 25°C. A combination of one female of each species and their offspring eliminated spider mites faster than the N. californicus alone, but slower than P. persimilis alone. Air relative humidities of 55% and 93% had no effect on predation. Both predator species cannibalised eggs and juveniles when spider mite numbers were low. In field experiments in 1996, fewer spider mites were recorded where P. persimilis was released, irrespective of the presence of N. californicus. Pest numbers on cv. 'First Gold' were lower than on cv. 'Herald'. No differences were recorded between the numbers of spider mite eggs in predator release treatments on 'First Gold', but fewer active stages of spider mites were recorded on plots with P. persimilis than controls soon after the time of peak pest population densities. On 'Herald', fewer spider mite active stages and eggs were recorded where predators were released than on untreated controls.  相似文献   

16.
Spin down in response to pesticides is a known phenomenon. This study aimed at investigating the effects of varying acaricide concentrations and acaricidal combinations on the rate of spin down and thread length, the fate of mites after spinning down and, thus, the implications of spin down in the context of mite control and the relationship between webbing and the efficacy of ovicides. In response to cyhexatin at a concentration of up to 100 mg dm-3 the rate of spin down did not change, but in response to higher concentrations it occurred earlier and ceased before the end of the experiment. The mean thread length did not vary with the concentration of acaricide and, whilst the proportion of mites spinning down increased with a cyhexatin concentration of up to 10 mg dm-3, it did not increase further with higher concentrations thereafter. It is therefore postulated that at any given time only a certain proportion of mites have a propensity to spin down. In combination with cyhexatin, only amitraz (at low concentrations) increased the proportion of mites spinning down, but amitraz (at higher concentrations), flubenzimine and permethrin all inhibited spin down, which may be a useful attribute in pesticide formulations. With regard to the fate of mites after spinning down, they were able to climb back up threads, but the proportion doing so was too small to be of practical importance. Mites which spin down and remain at the end of a thread die, but those which reached another leaf were able to spin down again and were more likely to do so if the leaf bore a cyhexatin residue. Mites reaching another leaf are also able to lay eggs, the number being greater if the leaf which they reach is untreated. Spin down is therefore likely to be of significant advantage to Tetranychus urticae in avoidance of the effects of acaricides. Finally, some eggs were observed to be laid on webbing and not to come into direct contact with clofentezine residues. The use of a fine water spray to simulate dew increased the ovitoxicity of clofentezine, probably by resuspension of the pesticide, but not of amitraz, which inhibits webbing production. The avoidance of acaricides should be added to the several known advantages of webbing production for spider mites. It is concluded that, all other factors being equal, pesticide formulations which inhibit spin down and webbing production are likely to be more effective in control programmes than those which stimulate such behaviour.  相似文献   

17.
Damage caused by two‐spotted spider mites (Tetranychus urticae) at harvest to yield, quality (measured in percentage α‐acids content) and cone infestation was assessed on hop cvs Hallertauer Magnum, Hallertauer Tradition and Perle. Acaricide‐untreated hop plants with known levels of T. urticae infestation were compared with neighbouring acaricide‐treated plants. Although in 24 of the 36 experimental harvests the untreated hop plants had spider mite infestations of > 100 mites leaf?1, yields and α‐acids content from the untreated plants were significantly lower than the treated plants in only four instances. However, although mite infestation of cones from untreated hops were significantly higher than acaricide‐treated plants in 27 of the 36 cases, in only one instance did that cause economic loss. Spider mite infestation levels of c. 90 mites leaf?1 are tolerable at harvest time with little or no risk of causing economic loss to hop growers.  相似文献   

18.
The dispersal behaviour of the predatory mite Phytoseiulus persimilis Athias-Henriot between bean plants was studied in a greenhouse. The aim of the study was to estimate the rate of predator emigration affected by different densities of Tetranychus urticae Koch and different numbers of between-plant connections (bridges). The results show that predators emigrate from a plant almost exclusively as a response to the local prey density whereas the loss rate (the per capita rate at which predators disappear from the system) also depends on the prey density on the surrounding plants, provided they are connected to the central plant by bridges. © Rapid Science Ltd. 1998  相似文献   

19.
Field data from 48 plots monitored during diverse weather conditions in two separate years were subjected to multiple regression analysis to determine which factors were related to spider-mite aerial dispersal. With the number of aerially dispersing mites as the dependent variable, partial regression coefficients (b) for mite population density and percent corn-leaf area infested with mites were positive, while those for percentNeozygites-infected mites and hours per week 90% r.h. were negative. When an aerial dispersal index (number of aerially dispersing mites/mites per plant) was used as the dependent variable, the partial regression coefficient for percent leaf area infested was positive, while coefficients for hours per week 90% r.h. and percentNeozygites-infected mites were negative. Mite aerial dispersal was greatest in predator-suppressed field plots under dry weather conditions. Mite aerial dispersal was substantially reduced in plots where moist weather conditions induced epizootics ofNeozygites floridana before corn plants became entirely infested with mites.  相似文献   

20.
The effects of triflumuron on the mortality, fecundity, and fertility of the two-spotted spider mite, Tetranychus urticae, were evaluated in the laboratory. No differences in toxicity for larvae, protonymphs and deutonymphs were observed, but immature stages were 3.8-times more susceptible than adults at the LC50. The compound exhibited a direct contact ovicidal activity influenced by eggs age. 48–72 hold eggs were significantly more sensitive than eggs of the other age classes. No hatch inhibition was observed in eggs laid by treated adult females using a sublethal dose at two physiological times (<12 and 48–72h old). However, fecundity decreased in younger treated females, but it increased in the older-ones. The toxicity for immatures and eggs, and the sublethal effects described suggest that triflumuron could be an interesting incorporation in integrated pest programs of T. urticae. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

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