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1.
Direct measurements of deep-brain and body-core temperature were performed on rats to determine the influence of cerebral blood flow (CBF) on brain temperature regulation under static and dynamic conditions. Static changes of CBF were achieved using different anesthetics (chloral hydrate, CH; α-chloralose, αCS; and isoflurane, IF) with αCS causing larger decreases in CBF than CH and IF; dynamic changes were achieved by inducing transient hypercapnia (5% CO2 in 40% O2 and 55% N2). Initial deep-brain/body-core temperature differentials were anesthetic-type dependent with the largest differential observed with rats under αCS anesthesia (ca. 2°C). Hypercapnia induction raised rat brain temperature under all three anesthesia regimes, but by different anesthetic-dependent amounts correlated with the initial differentials—αCS anesthesia resulted in the largest brain temperature increase (0.32 ± 0.08°C), while CH and IF anesthesia lead to smaller increases (0.12 ± 0.03 and 0.16 ± 0.05°C, respectively). The characteristic temperature transition time for the hypercapnia-induced temperature increase was 2–3 min under CH and IF anesthesia and ~4 min under αCS anesthesia. We conclude that both, the deep-brain/body-core temperature differential and the characteristic temperature transition time correlate with CBF: a lower CBF promotes higher deep-brain/body-core temperature differentials and, upon hypercapnia challenge, longer characteristic transition times to increased temperatures.  相似文献   

2.
The enthalpy of unfolding (ΔuH) of carbonic anhydrase II was determined by titrating the protein with acid and measuring the heat using isothermal titration calorimetry (ITC) in the temperature range of 5 to 59 °C. By combining the ITC results with our previous findings by differential scanning calorimetry (DSC) in the temperature range of 39 to 72 °C, the ΔuH dependence over a wide temperature range was obtained. The temperature dependence of the enthalpy displays significant curvature indicating that the heat capacity of unfolding (ΔuCp) is dependent on temperature. The T-derivative of ΔuCp was equal to 100 ± 30 J/(mol × K2), with the result that the ΔuCp is equal to 15.8 kJ/(mol × K) at 5 °C, 19.0 kJ/(mol × K) at 37 °C and 21.8 kJ/(mol × K) at 64 °C. The enthalpy of unfolding is zero at 17 °C. At lower temperatures, the ΔuH becomes exothermic.

This method of determining protein unfolding thermodynamics using acid-ITC, significantly widens the accessible T-range, provides direct estimate of the thermodynamic parameters at physiological temperature, and gives further insight into the third T-derivative of the Gibbs free energy of unfolding.  相似文献   


3.
Heat liberation in the brain was utilized as a direct signature of functional activation. We hypothesize that both temporal and spatial uncoupling between local cerebral blood flow (lCBF) and metabolic temperature components can be explored through the imaging of brain thermal gradients evoked by functional stimulation.

Surface cortical infrared (IR) images were obtained from 34 patients undergoing surgery for brain lesions under baseline conditions following peripheral nerve stimulation and, in some patients, during active behavioral tasks such as finger apposition and repetitive hand movements. An IR camera (0.02 °C sensitivity, 3–5 μm wavelength) was used to image local thermal gradients across the cerebral cortex by passively detecting IR emission.

Neural activation elicited reproducible temperature changes (0.04–0.09 °C) within the primary somatosensory cortex during median nerve stimulation and in the sensorimotor cortex during repetitive hand movements and finger tapping. The initial temperature responses were detected as early as 100–200 ms, the peak IR response occurred 5–7 s after stimulus onset.

Models of the relationship between evoked thermal gradients, lCBF and metabolic heat are proposed. Since the latencies of local metabolic and lCBF responses to stimulation vary by more than an order of magnitude, we are able to separate vascular-dependent and metabolic-dependent temperature components and thus create two discrete brain images, each reflecting distinct physiological mechanisms of functional activation. The resultant temperature profile reflects the balance between metabolism and lCBF, and therefore the degree of their functional uncoupling for the exposed and (possibly) for the intact normal human brain.  相似文献   


4.
1 Metabolic rates (Vo2), body temperature (Tb), and thermal conductance (C) were first determined in newly captured Maximowiczi's voles (Microtus maximowiczii) and Djungarian hamsters (Phodopus campbelli) from the Inner Mongolian grasslands at a temperature range from 5 to 35 °C.

2 The thermal neutral zone (TNZ) was between 25 and 32.5 °C for Maximowiczi's voles and between 25 and 30 °C for Djungarian hamsters. Mean Tb was 37.0±0.1 °C for voles and 36.2±0.1 °C for hamsters. Minimum thermal conductance was 0.172±0.004 ml O2/g h °C for voles and 0.148±0.003 ml O2/g h °C for hamsters.

3 The mean resting metabolic rate within TNZ was 2.21±0.05 ml O2/g h in voles and 2.01±0.07 ml O2/g h in hamsters. Nonshivering thermogenesis was 5.36±0.30 ml O2/g h for voles and 6.30±0.18 ml O2/g h for hamsters.

4 All these thermal physiological properties are adaptive for each species and are shaped by both macroenvironmental and microenvironmental conditions, food habits, phylogeny and other factors.

Keywords: Basal metabolic rate; Body temperature; Djungarian hamster (Phodopus campbelli); Maximowiczi's vole (Microtus maximowiczii); Nonshivering thermogenesis; Minimum thermal conductance  相似文献   


5.
Critical thermal maximum (CTmax) and body water losses were measured in first instar larvae of Gnorimus nobilis, Osmoderma eremita (Trichiinae) and Cetonischema aeruginosa (Cetoniinae) when air temperature was increased gradually (0.5 °C/min) from 20 °C to the critical thermal maximum (CTmax), in dry air (near 0% R.H.).

The CTmax was significantly lower in O. eremita (45.6±0.7 °C) than in G. nobilis (48.5±0.6) and C. aeruginosa (51.4±0.9 °C).

An increase of 10 °C (30–40 °C) induced a 2-fold increase of the water loss in C. aeruginosa and O. eremita (Q10=2.10±0.12 and 2.13±0.20, respectively). In the range from 40 to 45 °C to CTmax a strong increase of the water loss was observed in O. eremita and C. aeruginosa, respectively. Body water losses were significantly lower in C. aeruginosa than in O. eremita and G. nobilis over the range 20 °C—CTmax; no significant difference occurred between G. nobilis and O. eremita.  相似文献   


6.
A multi-segmental mathematical model of human thermoregulation was tested for its capability to predict individualized physiological responses. We compared the model predictions obtained for an average person with measured individual responses of subjects exposed to mild cold. Secondly, body composition (BC) data, the resting metabolic rate (MR), and the actual measured MR during the test were used as input into the model.

The data was obtained from 20 subjects (age: 19–36 years; BMI: 17–32 kg/m2). BC, MR, rectal and skin temperatures were measured for 1 h at 22 °C, followed by 3 h at 15 °C.

A mean bias of 1.8 °C, with a standard error of 0.7 °C, resulted for the mean skin temperature of an average person at 15 °C. When subjective BC and measured MR were incorporated the bias was −0.2±0.9 °C. For the hand-back skin temperature the bias ± standard error fell from 5.3±2.8 °C for an average person to 2.0±2.5 °C, when using individualized characteristics. Trunk skin temperatures were not significantly affected by the adjustments.

In conclusion, this study shows that on a group level predictions of skin temperatures can be improved when adopting individualized body characteristics and measured MR, but the predictions on an individual level were not improved.  相似文献   


7.
Intake of endophyte-infected tall fescue by cattle results in fescue toxicosis, which is characterized by increased hyperthermia during heat stress and concomitant reductions in feed intake and growth. Rats were monitored at 21 or 31 °C for short- or long-term periods to determine temporal changes associated with the intake of endophyte-infected (E+) or uninfected (E−) fescue seed diets. Core temperature only changed in rats fed E+ diet at 31 °C. Intake of E+ diet reduced feed intake, daily gain, and serum prolactin. There were temporal and thermal differences in the response to endophytic toxins, with short-term changes diminishing over time at 21 °C, but increasing for certain parameters at 31 °C.  相似文献   

8.
9.
Safflower (Charthamus tinctorius L.) seed press cake was pyrolysed in a fixed-bed reactor. The effects of pyrolysis temperature, heating rate and sweep gas flow rates on the yields of the products were investigated. Pyrolysis runs were performed using pyrolysis temperatures between 400 and 600 °C with heating rates of 10, 30 and 50 °C min−1. The obtained bio-char, gas and bio-oil yields ranged between 25 and 34 wt%, 19 and 25 wt%, and 28 and 36 wt%, respectively, at different pyrolysis conditions. The highest liquid yield was obtained at 500 °C pyrolysis temperature with a heating rate of 50 °C min−1 under the sweep gas of N2 with a flow rate of 100 cm3 min−1. Employing the higher heating rate of 50 °C min−1 results in maximum bio-oil yield, probably due to the decrease in mass transfer limitations. According to the results obtained under the conditions of this study, the effects of pyrolysis temperature and sweep gas flow rate are more significant than the effect of heating rate on the yields.  相似文献   

10.
《Journal of thermal biology》1999,24(5-6):379-383
The exposure of Wistar male rats (200±20 g) to high ambient temperature (38°C) for 20 and 60 min induced an equal decrease in hypothalamic, brain stem and hippocampal monoamine oxidase activity when compared to controls. The interscapular brown adipose tissue monoamine oxidase activity, as well as oxygen consumption and rectal temperature were increased only after a 60 min heat exposure. The adrenal function, assessed by dopamine-beta-hydroxylase activity and cholesterol concentration, was enhanced both after 20 and 60 min. In conclusion, heat induced the increase in adrenal function and interscapular brown adipose tissue monoamine oxidase activity, but the decrease in that of the brain.  相似文献   

11.
Male rats (450 g, n=11/group) were heated at an ambient temperature of 42°C until a rectal temperature of 42.8°C was attained. Rats, then received either saline (30°C)+tail ice water immersion (F+I) or saline (30°C)+tail ice water immersion+Nifedipine, a peripheral vasodilator, (F+I+N) to determine cooling rate effectiveness and survivability. The time to reach a rectal temperature of 42.8°C averaged 172 min in both groups resulting in similar heating rates (0.029°C/min). The cooling rates in group F+I and F+I+N were not significantly different from each other. We conclude that since Nifedipine did not improve cooling rates when combined with fluid+tail ice water immersion, its use as a cooling adjunct does not seem warranted.  相似文献   

12.
Thermal preference of the salamander Desmognathus fuscus was measured in a linear thermal gradient with floor temperatures ranging from 10 to 30°C. Salamanders were acclimated to 21±1°C and a 12 : 12 photoperiod with photophase centered at 1200 h for 8 weeks prior to being placed in the gradient. Substrate temperatures were measured under the salamanders’ stomachs from 1200 to 2400 h at 2 h intervals immediately after feeding and after seven days fasting. We found no selection for temperature in fasting or postprandial D. fuscus. We compared the rate at which D. fuscus cooled and heated with that of a control and found no significant difference. We determined the desiccation rate of D. fuscus at 16 and 26°C and found a significantly more rapid desiccation at 26°C.  相似文献   

13.
1. Skin and rectal temperatures were recorded continuously in 70 measurements during typical tasks of infantry and artillery training at 0 to −29 °C. The duration of the measurements varied from 55 min to 9.5 h.

2. The distribution of finger skin temperatures was quite similar at ambient temperature ranges 0 to −10 °C and −10 to −20 °C, while at −20 to −30 °C the finger temperatures were clearly lower.

3. At different ambient temperature ranges, 20–69% of finger temperatures were low enough to cause cold thermal sensations.

4. Sensation of cold was experienced at a finger temperature of 11.6±3.7 °C (mean±SD).  相似文献   


14.
A thermosensitive wild-type strain (PP201) of Rhizobium sp. (Cajanus) and its 14 heat-resistant mutants were characterized biochemically with regard to their cell surface (exopolysaccharides (EPSs) and lipopolysaccharides (LPSs)) properties and protein profile. Differences were observed between the parent strain and the mutants in all these parameters under high temperature conditions. At normal temperature (30 °C), only half of the mutant strains produced higher amounts of EPSs than the parent strain, but at 43 °C, all the mutants produced higher quantities of EPS. The LPS electrophoretic pattern of the parent strain PP201 and the heat-resistant mutants was almost identical at 30 °C. At 43 °C, the parent strain did not produce LPS but the mutants produced both kinds of LPSs. The protein electrophoretic pattern showed that the parent strain PP201 formed very few proteins at high temperature, whereas the mutants formed additional new proteins. A heat shock protein (Hsp) of 63–74 kDa was overproduced in all mutant strains.  相似文献   

15.
Controlled laboratory culture of Alexandrium catenella was used to determine the effects of a range of temperatures between 10 and 16 °C on the growth and saxitoxin content of this dinoflagellate, using strain ACC02 isolated from seawater at Aysen, XI Region, Southern Chile. Cell cultures were made using L1 culture medium at 30‰ salinity, and a photon flux density of 59.53 μmol m2 s−1. The results showed that the duration of the exponential growth phase was determined by the experimental temperature, with maximum cell concentrations obtained at 12 °C; significantly lower cell concentrations and growth rates were obtained at 16 °C. Cell dry weight and chlorophyll a values followed cell growth trends. The toxicity of A. catenella was variable at all the experimental temperatures, with a tendency towards having an inverse relation to temperature, with the highest values occurring at 10 °C and the lowest at 16 °C. The optimal range of temperature for the growth of the Chilean strain of A. catenella differed from rates reported for this species isolated at other latitudes, and was correlated with natural temperature conditions predominant in the environment from which it was isolated. The inverse relation of toxicity with temperature in the laboratory was broadly reflected in observations on the toxicity of this dinoflagellate in the field, and coincided with results from the literature.  相似文献   

16.
1. Each half rete from five Boer goats was perfused with water at 38 °C and flow rate of 2 ml min−1 while simultaneously perfusing the cavernous sinus with water at different temperatures and flow combinations and recording temperatures across the rete.

2. The minimum temperature difference across the rete was recorded at a cavernous sinus perfusion temperature of 37.8 °C and flow rate of 2 ml min−1.

3. Slopes of heat exchange increased threefold when the flow was increased four times.

4. These results support the idea that the rete is an obligate heat exchanger.

Keywords: Carotid rete; Selective brain cooling; Thermoregulation; Artiodactyls; Cavernous sinus  相似文献   


17.
Hsp70 content (ng Hsp70 μg total protein−1) in the liver and brain of control and adrenalectomized male rats was investigated by Western Blotting after heat stress (40°C) or endotoxin-induced fever (E. coli lipopolysaccharide injection). The increase in rectal temperature was higher after heat stress than after LPS injection. Heat stress affected Hsp70 content of the liver, but not of the brain; however adrenalectomy did not influence any parameter. These results suggest that, under these circumstances, there is no relationship between the hypothalamic–pituitary–adrenal axis and Hsp70 synthesis in liver and brain.  相似文献   

18.
1. Heat production (HP) and body core temperature (CT) where measured in 1- to 10-day old Muscovy ducklings and turkey chick, incubated during the last week before hatching at a lower (34.5 °C, LT-group) or at higher (38.5 °C, HT-group), than the normal temperature of 37.5 °C (control C-group).

2. In Muscovy ducklings, on the 1st day post-hatching HP was affected by exposure to low Ta of 10 °C Ta 28.2±3.9 W kg−1 in the LT-group vs. 18.1±2.4 W kg−1 in normal controls. On the same day, CT was higher (39.5±1.1 °C) in the HT- than in the CT-group (37.5±2.9 °C).

3. In turkeys, the relationships between Ta and HP could be described by parabola-like functions. Apart from the first day of life, the HP of the LT-group and the HT-group was higher than of the CT-group.

4. The low prenatal temperature of incubation resulted in a decrease of the preferred temperature in the LH-group and in an increase in the HT-group.

5. It is concluded that changes in incubation temperature at the end of embryonic development may induce an epigenetic temperature adaptation, which results in a long-lasting cold- and warm-adaptation in ducks but not in turkeys.

Keywords: Muscovy duck; Turkey; Epigenetic temperature adaptation; Imprinting; Determination; Heat production  相似文献   


19.
1. The present study examined the effect of the thermal state of the body (as reflected by rectal temperature) on cheek skin temperature and thermal resistance in active and inactive subjects.

2. Active subjects were exposed to a 30 min conditioning period (CP) (0 °C air with a 2 m/s wind), followed immediately by a 30 min experimental period (EP) (0 °C with a 5 m/s wind). Inactive subjects were exposed to a 30 min CP (22 °C air with no wind), followed immediately by a 45 min EP (0 °C air with a 4.5 m/s wind). The CP period was used to establish a core temperature difference between the active and inactive subjects prior to the start of EP. The 0 °C exposure was replaced with a −10 °C ambient air exposure and the experiment was repeated on a separate day. Subjects were comfortably dressed for each ambient condition.

3. Cheek skin temperature was not significantly higher in active subjects when compared to inactive subjects, but thermal resistance was higher in active subjects.

4. Cheek skin temperature and thermal resistance both decreased as ambient temperature decreased from 0 to −10 °C. The lower cheek thermal resistance at −10 °C may have been due to a greater cheek blood flow as a result of cold-induced vasodilation.

Keywords: Core temperature; Face skin temperature; Cheek thermal resistance; Cold exposure; Exercise  相似文献   


20.
1. Changes in MAO and antioxidative enzymes copper-zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD) and catalase (CAT) activities were examined in the hypothalamus and the hippocampus of Wistar rats exposed to cold stress (6 °C) for 180 min and heat stress (38 °C) for 60 min.

2. Extreme environmental temperatures caused stressor-specific changes in the hypothalamic and hippocampal MAO and antioxidative enzyme activities, being dependent on the stressor applied (cold or heat) but not on the brain region studied (the hypothalamus or hippocampus).

Keywords: Catalase; Cold stress; Heat stress; Hippocampus; Hypothalamus; Monoamine oxidase; Superoxide dismutase  相似文献   


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