Effectiveness of monoterpenes and phenylpropenes on Sitophilus oryzae L. (Coleoptera: Curculionidae) in stored wheat

Six monoterpenes, ((?)-citronellal, p-cymene, (?)-menthone, α-pinene, α-terpinene and (?)-terpinen-4-ol) and two phenylpropenes, (trans-cinnamaldehyde and eugenol) were tested for their insecticidal activity against the rice weevil, Sitophilus oryzae under laboratory conditions. The bioassays were carried out on wheat at the concentrations of 0.5, 1.0 and 5.0?g/kg. Adult mortality was assessed after 14?days of exposure. After this interval, the treated wheat was retained for an additional period of 90?days, in order to evaluate progeny production and wheat loss. At the concentrations of 0.5 and 1.0?g/kg, trans-cinnamaldehyde and eugenol achieved highest adult mortality. At 5.0?g/kg, all compounds except for p-cymene and α-pinene caused complete (100%) adult mortality. Generally, trans-cinnamaldehyde was the most potent compound causing complete inhibition of progeny at the three concentrations. Similarly, no progeny were observed in the wheat treated with (?)-citronellal, eugenol, p-cymene, (?)-menthone, and (?)-terpinen-4-ol at 5.0?g/kg after 45 and 90?days. Similar trends were noted for wheat weight loss and damage as the treatment with monoterpenes and phenylpropenes at the highest rate preserved the wheat intact and free from damage by S. oryzae for 90?days. Our findings suggest the tested compounds except p-cymene and α-pinene could be recommended for use as part of an integrated pest management program for S. oryzae control in stored wheat.     

21种植物提取物对玉米象的生物活性

为了筛选对粮库安全的植物源杀虫剂,研究了21种植物的4种溶剂（无水乙醇、丙酮、乙酸乙酯和石油醚）提取物对玉米象Sitophilus zeamais Motschulsky的驱避和触杀作用。结果表明,高良姜Alpinia officinarum石油醚提取物、黄姜Curcuma longa丙酮提取物和山鸡椒Litsea cubeba石油醚提取物对玉米象成虫的驱避作用显著,处理60 h平均驱避等级均达到Ⅴ级,平均驱避率分别为80.02%、80.60%和81.70%。在21种植物中,石菖蒲Acorus gramineus和巴豆Croton tiglium的4种溶剂提取物均对玉米象成虫的触杀作用明显,7.86 mg/cm²的剂量处理4天后的校正死亡率均达到90%以上。     

Distribution of the related weevil species Sitophilus oryzae and S. zeamais in Brazil

The genus Sitophilus （Coleoptera： Curculionidae） encompasses species of great economic importance as stored grain pests worldwide. Among these species, the maize and the rice weevils （Sitophilus zeamais and Sitophilus oryzae, respectively） are partic- ularly important in warmer climates. These two weevils exhibit closely morphological and ecological resemblance making difficult their proper identification and recognition of their distribution in grain-producing regions. Both species are recorded in South America and particularly in Brazil, but their respective distribution and prevalence were not yet assessed in the region. Therefore, several insect samples throughout Brazil were collected and subjected to morphological identification using male genitalia and also using molec- ular identification with species-specific primers designed for clear recognition of both the species. The primers were designed for the specific amplification of a gene fragment of the cytochrome oxidase subunit I, which exhibited high specificity during our prelimi- nary experiments with insects from six populations of known species （either S. zeamais or S. oryzae）. Both identification strategies provided the same results indicating preva- lence of the maize weevil S. zeamais throughout the country. Two hypotheses may explain such prevalence： （i） the likely host preference ofS. zeamais for maize because this is the most cultivated cereal in Brazil, and （ii） the prevalence ofS. zeamais in tropical regions as compared with S. oryzae, which is more disseminated in subtropical and temperate regions.     

Resistance of Maize Landraces to the Maize Weevil Sitophilus zeamais Motsch. (Coleoptera: Curculionidae)

The maize weevil Sitophilus zeamais Motsch. is an important pest of maize that attacks the grain both in the field and during storage. The damage caused by the maize weevil S. zeamais on maize landraces, Amarelo Antigo, Asteca, Caiano, Carioca, and Ferrinho, was evaluated by no-choice tests under laboratory conditions. The commercial varieties Sol da Manh?, BR 106, BR 451, and the synthetics PC 0203 and PC 9903 were evaluated for comparisons with the maize landraces. The parameters evaluated were susceptibility index, number of weevil progeny, development time, weevil progeny dry weight, and grain dry weight loss. The landraces were more susceptible to the maize weevil as compared to the commercial varieties. Based on the cluster analysis, two groups of susceptibility to the maize weevil were observed: one of more susceptible populations formed by local landraces and BR 451, and another less susceptible, with commercial varieties, synthetics, and the landrace Amarelo.     

Microstructure of mandibulate mouthparts in the greater rice weevil,Sitophilus zeamais (Coleoptera: Curculionidae)

The greater rice weevil, Sitophilus zeamais, is a serious pest of stored grain. It chews a hole in the grain and deposits an egg inside. For the purpose of excavating a tunnel, these weevils are equipped with effective mandibulate mouthparts for penetrating and boring holes. The mouthparts of the weevil are a long slender snout, which consist of a labrum, a pair of mandibles, a pair of maxillae and a labium. Mothparts exhibit typical morphology of phytophagous coleopteran beetles and have characteristics of chewing mouthparts. Mandibles are connected to the head capsule through dicondyous articulation, which allows movement along one single rotating axis. Both labrum and labium are fused to the snout and form the upper and lower lips, respectively. Along the depressed surface of the snout, a number of short sensory hairs are sparsely arranged. The distal apexes of the maxillary and labial palpi are deeply depressed into a sensillar field, and only one type of the basiconic sensilla, which function as chemo‐ or gustatory receptors, occurs in both sexes.     

Comparative composition of free,peptide and protein amino acids in symbiotic and aposymbiotic Sitophilus oryzae (coleoptera,curculionidae)

Free, peptide-bound and total amino acids were determined in symbiotic and aposymbiotic Sitophilus oryzae. The concentrations of free and peptide amino acids were higher, that of protein amino acids lower in aposymbiotic larvae (+30, +66 and −35%, respectively). In the free fraction, tyrosine concentration was the only one to be lower in aposymbiotic larvae (−41%) and could be a limiting factor for protein synthesis.In nymphs and adults, the compositions in symbiotic and aposymbiotic insects were very similar. A deficiency of tyrosine occurred however in aposymbiotic nymphs and of aspartate, glutamate, arginine, lysine and tyrosine in aposymbiotic adults. On the other hand, the composition in ovaries was unchanged.It is suggested that symbiotes could favour the synthesis of proteins in the larvae by increasing the efficiency of the conversion of phenylalanine in tyrosine.     

Pectin methylesterase from the rice weevil,Sitophilus oryzae (L.) (Coleoptera: Curculionidae): Purification and characterization

A pectin methylesterase was purified to apparent homogeneity from the adult rice weevil, Sitophilus oryzae (L.), by Q-Sepharose and S-Sepharose chromatographies followed by high-performance anion-exchange chromatography. The resulting preparation is the first pectin methylesterase which has been purified from any animal species, although at this point we cannot rule out the possibility that the enzyme is produced by a symbiotic microorganism. The molecular mass of the enzyme was estimated as 38 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This mass is similar to those of pectin methylesterases previously isolated from bacteria, fungi, and plants. The purified enzyme had a broad pH optimum between 6 and 7, which appears consistent with the enzyme's probable site of action, the gut.     

Repellent Efficacy of Caraway and Grapefruit Oils for Sitophilus oryzae (Coleoptera: Curculionidae)

This study examined the repellent efficacy of six essential oils extracted from caraway, clary sage, grapefruit, strawberry, thyme white, ylangylang, and their related volatile constituents against the adult rice weevil, Sitophilus oryzae using an olfactometer. The caraway and grapefruit oil showed the highest repellent efficacy against the rice weevil at a dose of 10μl. Gas Chromatography-Mass Spectrophotometer analysis revealed caraway oil to be rich in carvone and limonene, and grapefruit oil to be rich in limonene, β-myrcene and α-pinene. When the monoterpene was mixed in equal parts with the caraway and grapefruit essential oils, carvone with limonene in caraway oil demonstrated the highest repellent efficacy (96.7%). Limonene with α-pinene and β-myrcene in grapefruit showed strong repellent efficacy (86.4%) with synergistic effects on the S. oryzae.A mixture of caraway and grapefruit oils, as well as carvone and limonene, can be potent repellents that may be useful for controlling S. oryzae.     

Tropical Plant Extracts as Sources of Grain-Protectant Compounds Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae)

The toxicity of organic plant extracts to Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) was assessed for three tropical plant species: branches, leaves, and seeds of Annona montana (Annonaceae), branches of Aristolochia paulistana (Aristolochiaceae), and leaves and branches of Casearia sylvestris (Salicaceae). The screening assay resulted that the extracts of A. montana seeds obtained with hexane (LC₅₀?=?534.75 mg kg^?1 and LT₅₀?=?6.10 days) and with dichloromethane (LC₅₀?=?424.67 mg kg^?1 and LT₅₀?=?5.03 days) were the most promising treatments, followed by the extract prepared from A. montana leaves with hexane (LC₅₀?=?837.70 mg kg^?1 and LT₅₀?=?4.90 days). Moreover, extracts (at 1,500 mg kg^?1) prepared from C. sylvestris branches with dichloromethane and A. paulistana with hexane caused significant mortality (37% and 41.5%, respectively) beyond sublethal effects on S. zeamais. Therefore, based on the biological assays, extraction yield, and evaluation of the chromatographic profile of the crude extracts by TLC, the hexane extract of A. montana seeds was selected and fractioned using liquid-liquid partitioning. The hydroalcoholic fraction caused mortality of 55.5%, significantly superior to dichloromethane fraction, which caused 35.5% of mortality. Chemical analyses (¹H NMR, HPLC, and TLC) were performed, and the results showed the presence of alkaloids and acetogenins in both active fractions, which have been associated with bioactivity. Therefore, extracts prepared from A. montana seeds (especially) is an interesting source of new compounds with promising grain-protectant properties.     

Bioactivities of methyl allyl disulfide and diallyl trisulfide from essential oil of garlic to two species of stored-product pests, Sitophilus zeamais (Coleoptera: Curculionidae) and Tribolium castaneum (Coleoptera: Tenebrionidae)

Two of the major constituents of the essential oil of garlic, Allium sativum L., methyl allyl disulfide and diallyl trisulfide, were tested against Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst) for contact toxicity, fumigant toxicity, and antifeedant activity. The contact and fumigant toxicities of diallyl trisulfide were greater than that of methyl allyl disulfide to the adults of these two species of insects. These two compounds were also more toxic to T. castaneum adults than to S. zeamais adults. Older T. castaneum larvae were more susceptible to the contact toxicity of the two compound, whereas younger larvae were more susceptible to the fumigant toxicity of these compounds. Both compounds reduced egg hatching of T. castaneum and subsequent emergence of progeny. Diallyl trisulfide totally suppressed egg hatching at 0.32 mg/cm2, and larval and adult emergence at 0.08 mg/cm2. Methyl allyl disulfide significantly decreased the growth rate, food consumption, and food utilization of adults of both insect species, with feeding deterrence indices of 44% at 6.08 mg/g food for S. zeamais and 1.52 mg/g food for T. castaneum. However, it did not affect any nutritional indices of T. castaneum larvae. Diallyl trisulfide significantly reduced all of the nutritional indices in all of the insects tested. Feeding deterrence indices of 27 and 51% were obtained in S. zeamais adults and T. castaneum larvae, respectively, at the concentration of 2.98 mg/g food, whereas feeding deterrence of 85% was achieved in T. castaneum adults at a much lower concentration of 0.75 mg/g food. Hence, diallyl trisulfide is a more potent contact toxicant, fumigant and feeding deterrent than methyl allyl disulfide.     

Lethal and sublethal effects of rice polishing process on Sitophilus oryzae (Coleoptera: Curculionidae)

The mechanical impact of rice polishingon the rice weevil, Sitophilus oryzae (L.), and the subsequent postpolishing effects associated with rice quality were studied. "Brown" rice (not polished), "White (infest then polish)" rice, and "White (polish then infest)" rice were used with different polishing process intensities and different initial weevil densities. Weevil progeny were more numerous in Brown than in White (infest then polish) or White (polish then infest) rice. Polishing corresponding to a weight loss of 11% in rice generated an immediate mortality of approximately 40%; the subsequent mortality caused by rice quality was also 40% (total >80%). A polishing process corresponding to 14 and 16% of rice weight loss generated a drastic mortality in the weevil population (>95%). The progeny per adult weevil was similar with high and low initial weevil densities. The development of weevil progeny was also delayed by about 1 wk in White (infest then polish) rice compared with Brown rice. Furthermore, weevil adults of the progeny were significantly heavier in Brown than in White (infest then polish) or White (polish then infest) rice.     

Insecticidal activities of some medicinal plants against Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) on stored maize

A study was carried out to evaluate the insecticidal activity of Zanthoxylum zanthoxyloides, Aristolochia ringens, Garcinia kola, Morinda lucida, Euphorbia hirta, Croton zambesicus, Colocasia esculenta, Ficus exasperata and Tetrapleura tetraptera on Sitophilus zeamais at ambient temperature of 28?±?2?°C and 70?±?5% relative humidity. The plants powder was applied at 5, 10 and 20% (wt/wt) of maize grain. Weevil mortality, adult emergence, percentage damage, percentage reduction and weevil perforation index were observed. The result obtained showed that only the powder of Z. zanthoxyloides and A. ringens effect mortality on the weevil as they both achieved more than 50% weevil mortality within 96?h of application. However, powder of Z. zanthoxyloides had the highest mortality effect on the weevil as it achieved 100% within 72?h at 5%(wt/wt) and its effect was significantly (p?<?0.05) different from other powders. Also, powder of Z. zanthoxyloides, A. ringens and M. lucida showed a reduction capacity on the emergence of the adult weevil at 20%(wt/wt). Moreover, Z. zanthoxyloides prevented the emergence of the adult weevil even at 5%(wt/wt) but effect was not significantly different from A. ringens and M. lucida at 20%(wt/wt) as they both achieved 0% and 0.67% adult emergence. The powders also reduced the damage of the treated seeds. Z. zanthoxyloides prevented the damage of the seeds at 5, 10 and 20%(wt/wt) while A. ringens prevented the seed damage at 20%(wt/wt). However, effect of all the powders increased with increase in the powder concentration.     

Efficacy of cashew kernel extracts in the control of the maize weevil,Sitophilus zeamais (Coleoptera: Curculionidae)

Six different solvents namely: methanol, ethanol, water, acetone, pet-ether or n-hexane was used to extract the essential oils from cashew kernel. The extracted oils were evaluated on Sitophilus zeamais (Motschulsky 1855) for mortality, oviposition and adult emergence effects. The long-term storage and water absorption capacity of the treated maize grains was also investigated. Results showed that the steam distillate was most lethal on S. zeamais. The least potent extracts on S. zeamais were those extracted with methanol and ethanol. The extracts drastically reduced oviposition in S. zeamais. Maize grains treated with steam distillate extract had the lowest water absorption capacity, while the highest water absorption capacity was obtained in grains treated with ethanol and methanol. Extracts of steam distillate, n-hexane, petroleum ether and acetone completely prevented infestation and subsequence damage of the treated maize grains for a period of three months. In the grains treated with the methanol and ethanol extracts, damage of 28.61% and 6.24%, respectively were obtained. The results obtained reveal that extracts of cashew kernel are effective in controlling S. zeamais and could serve as an alternative to synthetic insecticides.     

Thermal treatments to increase acoustic detectability of Sitophilus oryzae (Coleoptera: Curculionidae) in stored grain.

Hidden infestations of stored-product insect larvae are detected most rapidly by acoustic techniques when the larvae are highly active. Larval activity is periodic, however, and it tends to decrease after the larvae are disturbed or cooled. Because of the practical need for rapid inspection of grain at commercial elevators, several heat treatments were tested as potential methods of increasing larval activity and improving the speed and reliability of acoustic detection under adverse conditions. Samples of grain infested with 4th instars of Sitophilus oryzae (L.) were exposed to different radiant and convective heat treatments after they had been conditioned at 11 degrees C, 17 degrees C, or room temperature for 12-24 h. Relative activity levels were evaluated over periods of 0-12 h based on the mean levels in a 15-min interval, 2 h after the beginning of a trial. In comparisons among treatments with precooled larvae, relative activity levels 5-10 min after brief heat pulses were 2-30 times higher than activity levels in precooled controls exposed only to ambient temperatures (25 degrees C). After 15-25 min, the relative activity levels of these heated larvae remained 2-5 times higher than those of the ambient controls. Brief movement disturbances inhibited activity for approximately 20 min at any temperature. These results suggest that, in general, larval detectability is enhanced if cool grain samples are warmed and all samples are left undisturbed for 15-20 min before inspection.     

Susceptibility of Sitophilus zeamais (Motsch.) (Coleoptera: Curculionidae) and Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) to Entomopathogenic Fungi from Ethiopia

The efficacy of 13 isolates of entomopathogenic fungi belonging to Beauveria , Metarhizium or Paecilomyces spp. was assessed against Sitophilus zeamais (Coleoptera: Curculionidae) and Prostephanus truncatus (Coleoptera: Bostrichidae) using a total immersion bioassay technique in the laboratory. Fungi were applied at concentrations of 1 ×10 7 and 1 ×10 8 conidia mL -1 for P. truncatus and S. zeamais , respectively. All isolates tested were virulent to P. truncatus (98-100% mortality, and median survival time (MST) ranged from 2.85-4.05 days). Metarhizium anisopliae and B. bassiana were also virulent to S. zeamais (92-100% mortality, MST ranged from 3.58-6.28 days). The isolate of Paecilomyces sp. was found to be the least virulent against S. zeamais , causing only 26.32 ±4.29% mortality with MST of 10.38 ±0.29 days. P. truncatus proved more susceptible to the entomopathogenic fungi tested than S. zeamais . One M. anisopliae (PPRC-EE) and three B. bassiana isolates (PPRC-HH, PPRC-9609 and PPRC-9614) were selected for further study and dose-mortality relationships were assessed on S. zeamais . The tested concentrations ranged from 1 ×10 4 -1 ×10 7 conidia mL -1 . M. anisopliae (PPRC-EE) showed the lowest LC 50 (3.39 ×10 5 conidia mL -1 ) followed by B. bassiana PPRC-HH (2.04 ×10 6 conidia mL -1 ). PPRC-9609 and PPRC-9614 showed slight differences in LC 50 but not at LC 90 . The results revealed the higher potency of M. anisopliae as compared with the B. bassiana isolates tested. The study suggests that the use of entomopathogenic fungi may hold promise as an alternative method to control pests of stored-products in Ethiopia.     

Imaging and automated detection of Sitophilus oryzae (Coleoptera: Curculionidae) pupae in hard red winter wheat

Computed tomography, an imaging technique commonly used for diagnosing internal human health ailments, uses multiple x-rays and sophisticated software to recreate a cross-sectional representation of a subject. The use of this technique to image hard red winter wheat, Triticum aestivm L., samples infested with pupae of Sitophilus oryzae (L.) was investigated. A software program was developed to rapidly recognize and quantify the infested kernels. Samples were imaged in a 7.6-cm (o.d.) plastic tube containing 0, 50, or 100 infested kernels per kg of wheat. Interkernel spaces were filled with corn oil so as to increase the contrast between voids inside kernels and voids among kernels. Automated image processing, using a custom C language software program, was conducted separately on each 100 g portion of the prepared samples. The average detection accuracy in the five infested kernels per 100-g samples was 94.4 +/- 7.3% (mean +/- SD, n = 10), whereas the average detection accuracy in the 10 infested kernels per 100-g sample was 87.3 +/- 7.9% (n = 10). Detection accuracy in the 10 infested kernels per 100-g samples was slightly less than the five infested kernels per 100-g samples because of some infested kernels overlapping with each other or air bubbles in the oil. A mean of 1.2 +/- 0.9 (n = 10) bubbles (per tube) was incorrectly classed as infested kernels in replicates containing no infested kernels. In light of these positive results, future studies should be conducted using additional grains, insect species, and life stages.