共查询到20条相似文献,搜索用时 31 毫秒
1.
Changhua Hu Yi Zou Wenting Zhao 《World journal of microbiology & biotechnology》2009,25(10):1705-1711
Effect of soybean oil on mycelial biomass and pleuromutilin biosynthesis by Pleurotus mutilis-04 was investigated in shake flask culture. The maximum pleuromutilin production and mycelial biomass were 8.32 ± 0.02 g l−1 and 49.10 ± 1.00 g l−1 when 20 g l−1 soybean oil was fed at 24 and 96 h respectively. A repeated fed-batch fermentation strategy with feeding 3 g l−1 soybean oil from 96 to 144 h at 24 h intervals was developed successfully to maintain mycelial growth and provide abundant
fatty acids for pleuromutilin biosynthesis. Compared with glucose as the sole carbon source, soybean oil was obviously beneficial
for the production of pleuromutilin. The results suggested that manipulation of metabolic regulation by soybean oil was an
effective way to enhance the production pleuromutilin. 相似文献
2.
Eliza Malinowska Wojciech Krzyczkowski Grzegorz Łapienis Franciszek Herold 《Journal of industrial microbiology & biotechnology》2009,36(12):1513-1527
The aim of this study was to optimize the culture medium used for the mycelial growth and production of intracellular polysaccharides
(IPS) and exopolysaccharides (EPS) in a submerged culture of Hericium erinaceum. Of the various factors examined, including carbon and nitrogen sources, vitamins, mineral elements, and initial pH, those
that proved to have a significant effect were then tested using a 24 central composite rotatable design (CCRD). Under the optimal culture conditions, the maximal yield of biomass reached 14.24 ± 0.45 g l−1 and was 1.85-fold higher than in the basal medium. The kinetics of EPS biosynthesis in a bioreactor showed that although
the highest yield of EPS (2.75 ± 0.27 g l−1) could be obtained on day 8, the process of biosynthesizing high molecular weight polysaccharides proceeded until the depletion
of the carbon source in the medium (after 14 days of cultivation). Our results could be very helpful in the large-scale production
of bioactive polysaccharides from H. erinaceum. 相似文献
3.
Hwang HS Lee SH Baek YM Kim SW Jeong YK Yun JW 《Applied microbiology and biotechnology》2008,78(3):419-429
In the present study, optimum culture conditions for the production of extracellular polysaccharides (EPS) in submerged culture
of an edible mushroom, Laetiporus sulphureus var. miniatus and their stimulatory effects on insulinoma cell (RINm5F) proliferation and insulin secretion were investigated. The maximum
mycelial growth (4.1 g l−1) and EPS production (0.6 g l−1) in submerged flask culture were achieved in a medium containing 30 g l−1 maltose, 2 g l−1 soy peptone, and 2 mM MnSO4·5H2O at an initial pH 2.0 and temperature 25°C. In the stirred-tank fermenter under optimized medium, the concentrations of mycelial
biomass and EPS reached a maximum level of 8.1 and 3.9 g l−1, respectively. Interestingly, supplementation of deep sea water (DSW) into the culture medium significantly increased both
mycelial biomass and EPS production by 4- and 6.7-fold at 70% (v/v) DSW medium, respectively. The EPS were proved to be glucose-rich polysaccharides and were able to increase proliferation
and insulin secretary function of rat insulinoma RINm5F cells, in a dose-dependent manner. In addition, EPS also strikingly
reduced the streptozotocin-induced apoptosis in RINm5F cells indicating the mode of the cytoprotective role of EPS on RINm5F
cells. 相似文献
4.
This study investigated the effects of surfactant additives and medium pH on mycelial morphology and exopolysaccharide (EPS)
production in liquid culture of a valuable medicinal fungus Cordyceps sinensis Cs-HK1. In the medium containing 20 g l−1 glucose and 6 g l−1 peptone as the sole nitrogen source, the Cs-HK1 fungal mycelia formed smooth and spherical pellets about 1.8-mm mean diameter.
The mycelial pellets became less uniform at pH (4.0–5.0) lower than the optimum (pH 6.0) or turned to filamentous form at
higher pH (8–9). Surfactants added to the medium inhibited pellet formation, resulting in smaller and looser pellets. Tween
80 exhibited a remarkable promoting effect on EPS production, increasing the EPS yield more than twofold at 1.5% (w/v), which
was most probably attributed to the stimulation of EPS biosynthesis and release from the fungal cells by Tween 80. 相似文献
5.
Praveen Nagella Hosakatte Niranjana Murthy 《Plant Cell, Tissue and Organ Culture》2011,104(1):119-124
Withania somnifera is an important medicinal plant that contains withanolides and withaferins, both bioactive compounds. We have tested the
effects of macroelements and nitrogen source in W. somnifera cell suspension cultures with the aim of optimizing the production of biomass and withanolide A. The effects of the macroelements
NH4NO3, KNO3, CaCl2, MgSO4 and KH2PO4 at concentrations of 0.0, 0.5, 1.0, 1.5 and 2.0× strength and of the nitrogen source [NH4
+/NO3
− (mM/mM) ratio of: 0.00/18.80, 7.19/18.80, 14.38/18.80, 21.57/18.80, 28.75/18.80, 14.38/0.00, 14.38/9.40, 14.38/18.80, 14.38/28.20,
and 14.38/37.60 (mM)] in Murashige and Skoog medium were tested for biomass and withanolide A production. The highest accumulation
of biomass [147.81 g l−1 fresh weight (FW) and 14.02 g l−1 (dry weight (DW)] was recorded in the medium containing a 0.5× concentration of NH4NO3, and the highest production of withanolide A content was recorded in the medium with 2.0× KNO3 (4.36 mg g−1 DW). The NH4
+/NO3
− ratio also influenced cell growth and withanolide A production, with both parameters being larger when the NO3
− concentration was higher than that of NH4
+. Maximum biomass growth (110.45 g l−1 FW and 9.29 g l−1 DW) was achieved at an NH4
+/NO3
− ratio of 7.19/18.80, while withanolide A production was greatest (3.96 mg g−1 DW) when the NH4
+/NO3
− ratio was 14.38/37.60 mM. 相似文献
6.
To screen stimulators from Chinese medicinal insects for mycelial growth and polysaccharides production of Ganoderma lucidum, G. lucidum was inoculated into the media with and without supplementation of medicinal insect extracts. The ethyl acetate extract of
Eupolyphaga sinensis at 55 mg l−1 lead to significant increase in both biomass and intracellular polysaccharides (IPS) concentration from 8.53 ± 0.41 to 14.16 ± 0.43
and 1.28 ± 0.09 to 2.13 ± 0.11 g l−1, respectively. In addition, the ethyl acetate extract of Catharsius molossus at 55 mg l−1 significantly enhanced extracellular polysaccharides (EPS) production; the EPS yield increased from 350.9 ± 14.1 to 475.1 ± 15.3 mg
l−1. There were no new components in the two types of polysaccharides obtained by the addition of the insect extracts. 相似文献
7.
Withanolides are biologically active secondary metabolites present in roots and leaves of Withania somnifera. In the present study, we have induced adventitious roots from leaf explants of W. somnifera for the production of withanolide-A, which is having pharmacological activities. Adventitious roots were induced directly
from leaf segments of W. somnifera on half strength Murashige and Skoog (MS) semisolid medium (0.8% agar) with 0.5 mg l−1 indole-3-butyric acid (IBA) and 30 g l−1 sucrose. Adventitious roots cultured in flasks using half strength MS liquid medium with 0.5 mg l−1 IBA and 30 g l−1 showed higher accumulation of biomass (108.48 g l−1FW and 10.76 g l−1 DW) and withanolide-A content (8.8 ± 0.20 mg g−1 DW) within five weeks. Nearly 11-fold increment of fresh biomass was evident in suspension cultures and adventitious root
biomass produced in suspension cultures possessed 21-fold higher withanolide-A content when compared with the leaves of natural
plants. An inoculum size of 10 g l−1 FW favoured the biomass accumulation and withanolide-A production in the tested range of 2.5, 5.0, 10.0 and 20.0 g l−1 FW. Among different media tested [Murashige and Skoog (MS), Gamborg’s (B5), Nitsch and Nitsch (NN) and Chu’s (N6)], MS medium
favoured both biomass accumulation and withanolide-A production. Half strength MS medium favoured the biomass accumulation
and withanolide-A production among the different strength MS medium tested (0.25, 0.5, 0.75, 1.0, 1.5 and 2.0). The current
results showed great potentiality of adventitious roots cultures for the production of withanolide-A. 相似文献
8.
Sachin Kumar Surendra P. Singh Indra M. Mishra Dilip K. Adhikari 《Journal of industrial microbiology & biotechnology》2009,36(12):1483-1489
A yeast strain Kluyveromyces sp. IIPE453 (MTCC 5314), isolated from soil samples collected from dumping sites of crushed sugarcane bagasse in Sugar Mill,
showed growth and fermentation efficiency at high temperatures ranging from 45°C to 50°C. The yeast strain was able to use
a wide range of substrates, such as glucose, xylose, mannose, galactose, arabinose, sucrose, and cellobiose, either for growth
or fermentation to ethanol. The strain also showed xylitol production from xylose. In batch fermentation, the strain showed
maximum ethanol concentration of 82 ± 0.5 g l−1 (10.4% v/v) on initial glucose concentration of 200 g l−1, and ethanol concentration of 1.75 ± 0.05 g l−1 as well as xylitol concentration of 11.5 ± 0.4 g l−1 on initial xylose concentration of 20 g l−1 at 50°C. The strain was capable of simultaneously using glucose and xylose in a mixture of glucose concentration of 75 g l−1 and xylose concentration of 25 g l−1, achieving maximum ethanol concentration of 38 ± 0.5 g l−1 and xylitol concentration of 14.5 ± 0.2 g l−1 in batch fermentation. High stability of the strain was observed in a continuous fermentation by feeding the mixture of glucose
concentration of 75 g l−1 and xylose concentration of 25 g l−1 by recycling the cells, achieving maximum ethanol concentration of 30.8 ± 6.2 g l−1 and xylitol concentration of 7.35 ± 3.3 g l−1 with ethanol productivity of 3.1 ± 0.6 g l−1 h−1 and xylitol productivity of 0.75 ± 0.35 g l−1 h−1, respectively. 相似文献
9.
The optimisation of submerged culture conditions and nutritional requirements was studied for the production of exopolysaccharide (EPS) fromPleurotus nebrodensis. The optimal temperature and initial pH for both mycelial growth and EPS production in shake flask cultures were 25 °C and 8.0, respectively. Maltose was found the most suitable carbon source for both mycelial biomass and EPS production. Yeast extract was favourable nitrogen source for both mycelial biomass and EPS production. Optimum concentration of each medium component was determined using the orthogonal matrix method. The optimal combination of the media constituents for mycelial growth and EPS production was as follows: 200 g l?1 bran, 25 g l?1 maltose, 3 g l?1 yeast extract, 1 g l?1 KH2PO4, 1 g l?1 MgSO4 7H2O. Under the optimal conditions, the mycelial biomass (4.13 g l?1) and EPS content (2.40 g l?1) ofPleurotus nebrodensis was 2.3 and 3.6 times compared to the control with basal medium respectively. 相似文献
10.
Mashitha Pise Jaishree Rudra Sunita Bundale Deovrat Begde Nandita Nashikkar Avinash Upadhyay 《In vitro cellular & developmental biology. Plant》2012,48(1):85-91
Asparagus racemosus is an important monocot medicinal plant that is in great demand for its steroidal saponins called shatavarins. This study
was initiated to optimize the conditions for production of shatavarins in cell cultures of A. racemosus in a modified Murashige and Skoog (MS) medium supplemented with six different combinations of growth regulators. Biomass
accumulation was correlated with saponin production over a 30-d culture cycle. Biomass and saponin accumulation patterns were
dependent on combinations of growth regulators and the pH of the medium. Maximum levels of saponin and biomass accumulation
were recorded on day 25 of the culture cycle within a pH range of 3.4 to 5.6. Total saponin produced by the in vitro cultures was 20-fold higher than amounts produced by cultivated plants. Saponin accumulation was not a biomass-associated
phenomenon; cultures which showed the highest biomass accumulation were not the highest saponin accumulators. Maximum biomass
(28.30 ± 0.29 g l−1) and maximum levels of shatavarin IV(11.48 ± 0.61 mg g−1) accumulation was found using a medium containing 2.0 mg l−1 2,4-D, 2 g l−1 casein hydrolysate and 0.005% pectinase. The highest levels of sarsapogenin, secreted and intracellular (4.02 ± 0.09 mg g−1), accumulated using a medium containing 1.0 mg l−1 NAA, 1.0 mg l−1 2,4-D, 0.5 mg l−1 BAP, 2 g l−1 casein hydrolysate and 0.005% pectinase, after 25 d. Shatavarins were secreted into the medium and can be isolated easily
for further purification. 相似文献
11.
In this study, alteration in morphology of submergedly cultured Antrodia camphorata ATCC 200183 including arthroconidia, mycelia, external and internal structures of pellets was investigated. Two optimization
models namely response surface methodology (RSM) and artificial neural network (ANN) were built to optimize the inoculum size
and medium components for intracellular triterpenoid production from A. camphorata. Root mean squares error, R
2, and standard error of prediction given by ANN model were 0.31%, 0.99%, and 0.63%, respectively, while RSM model gave 1.02%,
0.98%, and 2.08%, which indicated that fitness and prediction accuracy of ANN model was higher when compared to RSM model.
Furthermore, using genetic algorithm (GA), the input space of ANN model was optimized, and maximum triterpenoid production
of 62.84 mg l−1 was obtained at the GA-optimized concentrations of arthroconidia (1.78 × 105 ml−1) and medium components (glucose, 25.25 g l−1; peptone, 4.48 g l−1; and soybean flour, 2.74 g l−1). The triterpenoid production experimentally obtained using the ANN–GA designed medium was 64.79 ± 2.32 mg l−1 which was in agreement with the predicted value. The same optimization process may be used to optimize many environmental
and genetic factors such as temperature and agitation that can also affect the triterpenoid production from A. camphorata and to improve the production of bioactive metabolites from potent medicinal fungi by changing the fermentation parameters. 相似文献
12.
Haijun Wu Qingbiao Li Rui Lu Yuanpeng Wang Xiaoling Zhuang Ning He 《Journal of industrial microbiology & biotechnology》2010,37(11):1203-1209
The constant-rate fed-batch production of the polygalacturonic acid bioflocculant REA-11 was studied. A controlled sucrose-feeding
strategy resulted in a slight improvement in biomass and a 7% reduction in flocculating activity compared with the batch process.
When fed with a 3 g l−1 urea solution, the flocculating activity was enhanced to 720 U ml−1 in 36 h. High cell density (2.12 g l−1) and flocculating activity (820 U ml−1) were obtained in a 10-l fermentor by feeding with a sucrose-urea solution, with values of nearly two times and 50% higher
than those of the batch process, respectively. Moreover, the residual sucrose declined to 2.4 g l−1, and residual urea decreased to 0.03 g l−1. Even higher flocculating activity of 920 U ml−1 and biomass of 3.26 g l−1 were obtained by feeding with a sucrose-urea solution in a pilot scale fermentation process, indicating the potential industrial
utility of this constant-rate feeding strategy in bioflocculant production by Corynebacterium glutamicum. 相似文献
13.
A medicinal mushroom, Phellinus linteus, was successfully cultivated using a cheese-processing waste, whey, and the optimal bioconversion conditions for the maximum
mycelial growth rate was also estimated through solid-state cultivation experiments. Response surface analysis with a face-centered
design (center point replication = 5) was applied to statistically approximate the simultaneous effects of the three variables,
i.e., substrate concentration (10–30 g lactose l−1), temperature (20–30°C), and pH (4–6), on the mycelial growth rate of P. linteus. The following is a partial cubic model where η is the mycelial growth rate (K
r
) and x
k
is the corresponding variable term (k = substrate concentration, temperature, and pH in order): η = −23.8 + 8.67 × 10−2
x
1 + 1.48x
2 + 1.77x
3 + 8.00 × 10−4
x
1
x
2 + 7.25 × 10−2
x
1
x
3 + 5.13 × 10−2
x
2
x
3 −1.28 × 10−2
x
12 –3.18 × 10−2
x
22. −2.64 × 10−1
x
32 −3.28 × 10−3
x
1
x
2
x
3 + 4.68 × 10−4
x
12
x
2. The produced response surface model proved to be significant (r
2 > 0.99, P-value <0.0001, coefficient of variation <5%) to describe the explored space. Temperature was found to be the most significant
factor of dominant effects on the mycelial growth rate, and other variables such as temperature2, pH, pH2, and (substrate concentration2 × temperature) also showed significant effects on the model output. The maximum mycelial growth rate was predicted to be
2.80 mm d−1 at 29.7 g lactose l−1, 26.2°C, and pH 5. Our results proved a good potential of whey to serve as an alternative growth medium for cultivating P. linteus mycelia. This may provide another potential for managing this nutrient-rich waste in a cost-effective way. 相似文献
14.
Teresa Manso Carla Nunes Sara Raposo Maria Emília Lima-Costa 《Journal of industrial microbiology & biotechnology》2010,37(11):1145-1155
Large-scale production has been the major obstacle to the success of many biopesticides. The spreading of microbial biocontrol
agents against postharvest disease, as a safe and environmentally friendly alternative to synthetic fungicides, is quite dependent
on their industrial mass production from low-cost raw materials. Considerable interest has been shown in using agricultural
waste products and by-products from food industry as nitrogen and carbon sources. In this work, carob pulp aqueous extracts
were used as carbon source in the production of the biocontrol agent Pantoea agglomerans PBC-1. Optimal sugar extraction was achieved at a solid/liquid ratio of 1:10 (w/v), at 25°C, for 1 h. Batch experiments were
performed in shake flasks, at different concentrations and in stirred reactors at two initial inoculums concentrations, 106 and 107 cfu ml−1. The initial sugar concentration of 5 g l−1 allowed rapid growth (0.16 h−1) and high biomass productivity (0.28 g l−1 h−1) and was chosen as the value for use in stirred reactor experiments. After 22 and 32 h of fermentation the viable population
reached was 3.2 × 109 and 6.2 × 109 cfu ml−1 in the fermenter inoculated at 106 cfu ml−1 and 2.7 × 109 and 6.7 × 109 cfu ml−1 in the bioreactor inoculated at 107 cfu ml−1. A 78% reduction of the pathogen incidence was achieved with PBC-1 at 1 × 108 cfu ml−1, grown in medium with carob extracts, on artificially wounded apples stored after 7 days at 25°C against P. expansum. 相似文献
15.
Smita Srivastava Ashok Kumar Srivastava 《In vitro cellular & developmental biology. Plant》2012,48(1):73-84
Azadirachtin, a well-known biopesticide, is a secondary metabolite extracted from the seeds of Azadirachta indica. In the present study, azadirachtin was produced in hairy roots of A. indica, generated by Agrobacterium rhizogenes-mediated transformation of leaf explants. Liquid cultures of A. indica hairy roots were developed with a liquid-to-flask volume ratio of 0.15. The kinetics of growth and azadirachtin production
were established in a basal plant growth medium containing MS medium major and minor salts, Gamborg’s medium vitamins, and
30 g l−1 sucrose. The highest azadirachtin accumulation in the hairy roots (up to 3.3 mg g−1) and azadirachtin production (∼44 mg l−1) was obtained on Day 25 of the growth cycle, with a biomass production of 13.3 g l−1 dry weight. To enhance the production of azadirachtin, a Plackett–Burman experimental design protocol was used to identify
key medium nutrients and concentrations to support high root biomass production and azadirachtin accumulation in hairy roots.
The optimal nutrients and concentrations were as follows: 40 g l−1 sucrose, 0.19 g l−1 potassium dihydrogen phosphate, 3.1 g l−1 potassium nitrate, and 0.41 g l−1 magnesium sulfate. Concentrations were determined by a central composite design protocol and verified in shake-flask cultivation.
The optimized medium composition yielded a root biomass production of 14.2 g l−1 and azadirachtin accumulation of 5.2 mg g−1, which was equivalent to an overall azadirachtin production of 73.84 mg l−1, 68% more than that obtained under non-optimized conditions. 相似文献
16.
Maltose and yeast extract were the most favourable carbon and nitrogen sources for exopolysaccharide production by submerged
culture of Shiraia bambusicola WZ-003, and initial maltose and yeast extract concentrations were at 30 and 3 g l−1, respectively. Plant oils could increase the mycelial growth and exopolysaccharide production in tested concentration. K+ and Mg2+ could enhance the mycelial growth and exopolysaccharide biosynthesis. The optimal cultivation temperature and initial pH
were found to be 26°C and 6.0, respectively. Exopolysaccharide concentration reached 0.53 g l−1 in 15-l fermenter under optimal nutritional conditions. 相似文献
17.
The present study describes the potential of in vitro grown adventitious roots of Hypericum perforatum L. commonly known as St. John’s wort at low nutrient and auxin levels in the liquid medium for micropropagation. Roots were
regenerated from shoot-derived callus on MS medium containing 4.0 mg l−1 Indole-3 acetic acid (IAA). IAA and Indole-3 butyric acid (IBA) were equally effective for the induction of roots from shoot
cultures. Half strength MS medium containing 1.0 mg l−1 IAA was most found suitable for culturing roots in liquid medium. A total biomass of 4.13 ± 0.67 g comprising 226 ± 34.4
shoots and shoot buds along with roots was obtained per culture starting with 200 mg roots inoculum. Pretreatment with kinetin
(2.0 mg l−1) enhanced the shoot multiplication. Shoots proliferated profusely from excised roots in static liquid medium supported with
glass bead matrix. Growtek™ vessel was found suitable and cost effective system for high throughput plantlet production. In vitro grown roots regardless
of their source of origin were an excellent and easy to handle source of explant for aseptic production of plantlets without
loosing the morphogenetic potential over the generations. The plants exhibited 84–99% similarity among themselves through
RAPD. The in vitro shoots produced can either be multiplied or rooted perpetually, and alternatively they can also be explored
for the in vitro production of hypericin and hyperforin. 相似文献
18.
Laura Pinilla Rodrigo Torres Claudia Ortiz 《World journal of microbiology & biotechnology》2011,27(11):2521-2528
Two wild strains of Zymomonas mobilis were isolated (named as ML1 and ML2) from sugar cane molasses obtained from different farms of Santander, Colombia. Initially,
selection of the best ethanol-producer strains was carried out using ethanol production parameters obtained with a commercial
strain Z. mobilis DSM 3580. Three isolated strains were cultivated in a culture medium containing yeast extract, peptone, glucose and salts,
at pH 6 and 32°C with stirring rate of 65 rpm during 62 h. The best results of ethanol production were obtained with the native
strain ML1, reaching a maximum ethanol concentration of 79.78 g l−1. ML1 and ML2 strains were identified as Z.
mobilis, according to the morphology, biochemical tests and molecular characterization by PCR of specific DNA sequences from Z. mobilis. Subsequently, the effect of different nitrogen sources on production of ethanol was evaluated. The best results were obtained
using urea at a 0.73 g/l. In this case, maximum concentration of ethanol was 83.81 g l−1, with kinetic parameters of yield of ethanol on biomass (YP/X) = 69.01(g g−1), maximum volumetric productivity of ethanol (Qpmax) = 2.28 (g l−1 h−1), specific productivity of ethanol (qP) = 3.54 (h−1) and specific growth rate (μ) = 0.12 h−1. Finally, we studied the effect of different culture conditions (pH, temperature, stirring, C/N ratio) with a Placket-Burman′s
experimental design. This optimization indicated that the most significant variables were temperature and stirring. In the
best culture conditions a significant increase in all variables of response was achieved, reaching a maximum ethanol concentration
of 93.55 g l−1. 相似文献
19.
Fermentation of biomass derived synthesis gas to ethanol is a sustainable approach that can provide more usable energy and
environmental benefits than food-based biofuels. The effects of various medium components on ethanol production by Clostridium ragsdalei utilizing syngas components (CO:CO2) were investigated, and corn steep liquor (CSL) was used as an inexpensive nutrient source for ethanol production by C. ragsdalei. Elimination of Mg2+, NH4
+ and PO4
3− decreased ethanol production from 38 to 3.7, 23 and 5.93 mM, respectively. Eliminating Na+, Ca2+, and K+ or increasing Ca2+, Mg2+, K+, NH4
+ and PO4
3− concentrations had no effect on ethanol production. However, increased Na+ concentration (171 mM) inhibited growth and ethanol production. Yeast extract (0.5 g l−1) and trace metals were necessary for growth of C. ragsdalei. CSL alone did not support growth and ethanol production. Nutrients limiting in CSL were trace metals, NH4
+ and reducing agent (Cys: cysteine sulfide). Supplementation of trace metals, NH4
+ and CyS to CSL (20 g l−1, wet weight basis) yielded better growth and similar ethanol production as compared to control medium. Using 10 g l−1, the nutritional limitation led to reduced ethanol production. Higher concentrations of CSL (50 and 100 g l−1) were inhibitory for cell growth and ethanol production. The CSL could replace yeast extract, vitamins and minerals (excluding
NH4
+). The optimized CSL medium produced 120 and 50 mM of ethanol and acetate, respectively. The CSL could provide as an inexpensive
source of most of the nutrients required for the syngas fermentation, and thus could improve the economics of ethanol production
from biomass derived synthesis gas by C. ragsdalei. 相似文献
20.
Mucor indicus can be used to produce ethanol from a variety of sugars, including pentose’s. An extract of it, produced by autolysis, could
replace yeast extract in culture medium with improved production of ethanol. At 10 g l−1, the extract gave a higher ethanol yield (0.47 g g−1) and productivity (0.71 g l−1 h−1) compared to medium containing yeast extract (yield 0.45 g g−1; productivity 0.67 g l−1 h−1). 相似文献