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1.
Cupressus chenggiana S. Y. Hu (Cupressaceae) is an endemic and endangered conifer species in southwest China. In order to study the population
genetics and design the effective conservation methods, we aimed to develop microsatellite primers for this species in the
present study. We developed eight new microsatellite loci for this species through biotin capture method. Polymorphism of
each locus was further assessed in 18 individuals from three geographically distant populations. The number of alleles per
locus ranged from 6 to 11 with an average of 8.13. The observed and expected heterozygosities ranged from 0.219 to 0.296 and
from 0.374 to 0.470, with averages of 0.254 and 0.417, respectively. We further found that three of nine microsatellite loci
developed previously for another congeneric species showed polymorphic banding patters. We performed primer-crossing tests
of these loci in the other two congeneric species which are closely related to C. chenggiana (C. gigantea and C. duclouxiana). These microsatellite markers would be effective for analyzing genetic diversity and population genetic structure of this
species and its morphological differentiation with the close relatives. 相似文献
2.
Queule (Gomortega keule) is an endangered, Chilean tree in the endemic, monotypic family Gomortegaceae. Eight polymorphic microsatellite loci were developed and characterized for this species. The number of alleles per locus ranged from four to 12 and the observed heterozygosity ranged from 0.2 to 1.0. These microsatellite markers will be valuable tools for population genetics studies and conservation planning. 相似文献
3.
Phaedranassa tunguraguae is an endangered species endemic to Ecuador. Eight highly polymorphic microsatellite loci were isolated from an enriched genomic library for this species. Levels of polymorphism were evaluated using a total of 31 individuals from a single natural population. An average of 14.1 alleles per locus was detected, and observed heterozygosity ranged from 0.387 to 0.903. All but one locus depart significantly from Hardy–Weinberg equilibrium. These loci are the first microsatellite primers isolated for Amaryllidaceae and will be utilized to investigate patterns of genetic variation of P. tunguraguae, which will contribute data relevant to the conservation of the species. 相似文献
4.
Development and characterization of 24 microsatellite markers in Primula tosaensis,an endangered primrose,using MiSeq
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Masaya Yamamoto Yoshihiro Handa Hiroki Aihara Hiroaki Setoguchi 《Plant Species Biology》2018,33(1):77-80
Primula tosaensis (Primulaceae) is an endangered primrose endemic to Japan. In this study, 24 novel microsatellite markers were developed using Illumina MiSeq sequencing to facilitate conservation of this endangered species. The genetic diversity and polymorphisms of these novel markers were measured in 32 individuals from a wild P. tosaensis population. The number of alleles and expected heterozygosities ranged from 2 to 5 (mean = 2.8) and from 0.119 to 0.724 (mean = 0.395), respectively. All loci were in Hardy–Weinberg equilibrium. The markers developed in this study will provide a powerful and practical tool for investigating the population structure and genetic diversity of P. tosaensis. 相似文献
5.
N. Mesquita C. Cunha B. Hnfling G. R. Carvalho L. Z‐Z R. Tenreiro M. M. Coelho 《Molecular ecology resources》2003,3(4):572-574
Here we describe the isolation and characterization of six polymorphic microsatellite loci for Squalius aradensis. The number of observed alleles per locus ranged from three to 16 and the observed heterozygosity from 0.22 to 0.95. These primers will be useful in determining the population structure of S. aradensis and for conservation genetics of this endangered and endemic species. Furthermore, successful cross‐species amplification in S. alburnoides and Chondrostoma lusitanicum suggests that a wider amplification of these markers is possible. 相似文献
6.
J. KHUDAMRONGSAWAT L. S. HEATH H. E. HEATH P. M. HARRIS 《Molecular ecology resources》2007,7(5):811-813
The endangered vermilion darter (Etheostoma chermocki) is endemic to the Black Warrior River system in the Mobile Basin in Alabama. Restoration and conservation of this species require an understanding of its population genetic structure, which can be characterized using microsatellite DNA. Nine microsatellite loci were developed; eight loci were polymorphic. Although observed heterozygosity was lower than expected heterozygosity in most polymorphic loci, only one locus showed significant deviation from Hardy–Weinberg equilibrium. These nine markers were tested in an additional 24 species of Etheostoma and appear to have sufficient allelic variation to be useful in studies of population genetic structure. 相似文献
7.
INGA ZEISSET THOMAS DAMM ALS JOSEF SETTELE JACOBUS J. BOOMSMA 《Molecular ecology resources》2005,5(1):165-168
We developed microsatellite markers for Maculinea nausithous and Maculinea alcon, two of five species of endangered large blue butterflies found in Europe. Two separate microsatellite libraries were constructed. Eleven markers were developed for M. nausithous and one for M. alcon. The primers were tested on both species as well as on the three other European Maculinea species. The number of alleles per locus ranged from two to 14. These markers will be useful tools for population genetic studies of Maculinea species. 相似文献
8.
Qiushi Yu Dongyuan Zhang Dongshi Wan Haiyan Xu Changming Zhao 《Conservation Genetics》2008,9(4):1019-1022
Pugionium (Brassicaceae) is a small Central-Asian endemic genus with four tentative species described before. All of them grow in the
desert habitat and have been considered as important psammophytes for evolutionary studies and ecological restorations. However,
the wild resources of these species have been declined because of overexploitation. In this study, 12 polymorphic microsatellite
loci have been developed for P. dolabratum Maxim, and the performance of primer pairs amplifying these loci in related three species has also been tested. The number
of alleles in 18 individuals from 13 geographically distant populations ranged from three to seven per locus. The observed
and expected heterozygosities varied from 0.17 to 0.30 and from 0.32 to 0.48, respectively. We further performed cross-priming
tests in the remaining three species of this genus, P. cornutum, P. calcaratum and P. cristatum. These newly recovered microsatellite loci will be useful for studying the population genetics and adaptive evolution of
these endangered psammophytes in the desert. 相似文献
9.
Harue Abe Yoko Nishikawa Takashi Shimamura Ken Sato Yoshihisa Suyama 《American journal of botany》2012,99(12):e484-e486
• Premise of the study: Nuclear microsatellite primers were developed to analyze the clonal diversity and population genetic structure of the endemic polyploid herb Callianthemum miyabeanum. • Methods and Results: Using a protocol for constructing microsatellite-enriched libraries, 15 primer sets were developed for use in C. miyabeanum. The number of alleles found ranged from five to 22. The estimated range of expected heterozygosities was 0.574 to 0.907, and the Shannon–Weiner diversity index ranged from 1.061 to 2.733. Cross-amplification of all loci was also successful in the closely related endemic species C. kirigishiense and C. hondoense. • Conclusions: The development of these microsatellite loci will facilitate a deeper understanding of the genetic diversity, mode of reproduction, and population structure of not only C. miyabeanum, but also the other Callianthemum species endemic to Japan. 相似文献
10.
Abdul Muneer PM Gopalakrishnan A Musammilu KK Mohindra V Lal KK Basheer VS Lakra WS 《Molecular biology reports》2009,36(7):1779-1791
Random amplified polymorphic DNA (RAPD) and microsatellite markers were applied to evaluate the genetic variation in endemic
and endangered yellow catfish, Horabagrus
brachysoma sampled from three geographic locations of Western Ghat, South India river systems. In RAPD, of 32 10-mer RAPD primers screened
initially, 10 were chosen and used in a comparative analysis of H. brachysoma collected from Meenachil, Chalakkudy and Nethravathi River systems. Of the 124 total RAPD fragments amplified, 49 (39.51%)
were found to be shared by individuals of all 3 populations. The remaining 75 fragments were found to be polymorphic (60.48%).
In microsatellites, six polymorphic microsatellite loci were identified by using primers developed for Pangasius
hypophthalmus, Clarias
macrocephalus and Clarias
gariepinus. The identified loci were confirmed as microsatellite by sequencing after making a clone. The nucleotide sequences of 6 loci
were published in NCBI genbank. The number of alleles across the six loci ranged from 4 to 7 and heterozygosities ranged from
0.07 to 0.93. The mean number of alleles and effective number of alleles per locus were 5.00 and 3.314, respectively. The
average heterozygosity across all investigated samples was 0.72, indicating a significant deficiency of heterozygotes in this
species. RAPD and microsatellite methods reported a high degree of gene diversity and genetic distances depicted by UPGMA
dendrograms among the populations of H. brachysoma. 相似文献
11.
JESS W. JONES MELANIE CULVER VICTOR DAVID JENNIFER STRUTHERS NATHAN A. JOHNSON RICHARD J. NEVES STEPHEN J. O'BRIEN ERIC M. HALLERMAN 《Molecular ecology resources》2004,4(4):649-652
Primers for 10 polymorphic microsatellite loci were developed and characterized for the endangered oyster mussel Epioblasma capsaeformis from the Clinch River, Tennessee. Microsatellite loci also were tested in four other populations or species. Amplification was successful for most loci in these closely related endangered species or populations; therefore, a high level of flanking sequence similarity was inferred for this group of species and populations. Allelic diversity ranged from nine to 20 alleles/locus, and averaged 13.6/locus. This study demonstrated the feasibility of using polymerase chain reaction (PCR) primers to amplify microsatellite loci across freshwater mussel species to conduct population genetics studies. 相似文献
12.
Chinese alligator (Alligator sinensis) is a critically endangered species endemic to China. In this study, we developed 11 novel microsatellite loci for this rare
species and applied them to examine genetic variation of indigenous alligators from Changxing Nature Reserve and America-born
Chinese alligators. The 11 polymorphic microsatellites presented a total of 31 alleles among 57 individuals scored, yielding
an average of 2.82 alleles per locus. One allele was unique to the American population but four private alleles were detected
in the Changxing population. The average expected and observed heterozygosities were 0.400 and 0.482 for the Changxing alligators
and 0.520 and 0.621 for the America-born individuals, respectively. These microsatellite markers would be useful tools in
the genetic examination of this endangered species. 相似文献
13.
LINDA M. McINNES IAN R. DADOUR MEREDITH E. STEWART WILLIAM G. F. DITCHAM PETER MAWSON PETER B. S. SPENCER 《Molecular ecology resources》2005,5(3):504-506
Microsatellite loci were isolated from Carnaby's black cockatoo (Calyptorhynchus latirostris: Aves), a highly valued, endangered, and endemic species of bird from Western Australia. This study describes three dinucleotide and one tetranucleotide microsatellite loci for which the primers produced clear and polymorphic amplification patterns with between two and nine alleles and moderate levels of variability. Two additional dinucleotide markers which were monomorphic in the Carnaby's cockatoo were able to amplify and were polymorphic in two other species of black cockatoo, greatly increasing the utility of these markers. 相似文献
14.
Ceropegia thaithongiae sp. nov. (Apocynaceae,Asclepiadoideae) from northern Thailand and preliminary observations on impacts of climate change on its only known population
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A new pendulous herb, Ceropegia thaithongiae Kidyoo (Apocynaceae, Asclepiadoideae), is described from northern Thailand. This plant is strictly endemic to Chiang Dao Wildlife Sanctuary and critically endangered owing to it living in an ecosystem affected by frequent natural disturbances. It is here described, illustrated and compared to the similar species, C. mairei. Both species have fusiform rootstocks, 1–2‐flowered cymes, deeply bifid outer corona lobes and linear, hairy inner corona lobes. However, the new species can be distinguished by its glabrous stem, leaf covered with hairs on both surfaces and glabrous corolla tube and lobes. 相似文献
15.
Xin Zhou Yu Xu Jianghong Ran Bisong Yue Lusha Cao Jing Li 《European Journal of Wildlife Research》2009,55(1):81-83
Microsatellite loci for the buff-throated partridge (Tetraophasis szechenyii), an endemic pheasant species of China, are here described for the first time. Twenty-five microsatellite markers from chicken
and Japanese quail were tested on buff-throated partridge DNA by means of cross-amplification. Twenty (80%) primers yielded
specific products and polymorphisms were tested in a wild population of buff-throated partridge. Twelve (48%) proved to be
polymorphic with an average of two alleles per locus. Current results of buff-throated partridge microsatellites loci could
be employed in population genetic studies and on other endangered pheasant species. 相似文献
16.
A microsatellite library was developed using genomic DNA of the endangered dusky gopher frog, Rana sevosa. Polymerase chain reaction (PCR) primers and conditions are presented for R. sevosa (eight loci) and two sister taxa — other gopher frogs, Rana capito (seven loci) and crawfish frogs, Rana areolata (three loci). Polymorphism of each microsatellite locus was evaluated for each species. All loci have moderate to high genetic variation in terms of allelic richness (four to 10 alleles per locus), observed heterozygosity (0.595–0.946), and expected heterozygosity (0.531–0.856). 相似文献
17.
Metrosideros boninensis is an endangered endemic plant species from the Bonin Islands. We isolated and characterized nine microsatellite loci in
this species. The expected heterozygosities of these nine markers ranged from 0.127 to 0.768. These markers described here
will be useful for investigating the genetic diversity, genetic structure and gene flow, and planning for conservation of
M. boninensis. 相似文献
18.
The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species. 相似文献
19.
Min Zhang Zheng-Feng Wang Shu-Guang Jian Wan-Hui Ye Hong-Lin Cao Peng Zhu Ling Li 《Conservation Genetics》2009,10(4):1175-1176
Cycas hainanensis is an endangered cycad species endemic to Hainan Island, China. To enrich our scientific conservation for this species, we
developed eight microsatellite markers using repetitive DNA enriched libraries. The number of alleles per microsatellite locus
varied from 8 to 17. The expected (H
E
) and observed (H
O
) heterozygosities varied from 0.4754 to 0.8846 and from 0.3636 to 0.9600, respectively. These markers will be employed to
determine whether the ex situ C. hainanensis individuals in Sourh China Botanical Garden capture a representative portion of genetic diversity of the wild populations. 相似文献
20.
Andriamahery Razafindrakoto Erwan Quéméré Gary D. Shore Susie M. McGuire Edward E. LouisJr. Rick A. Brenneman 《Conservation Genetics》2008,9(4):1027-1031
The golden-crowned sifaka (Propithecus tattersalli) is one of the most critically endangered species of Propithecus endemic to a limited area in the Daraina region of north-eastern Madagascar. This species is endangered due to habitat loss
and fragmentation, a consequence of deforestation. Twenty nuclear microsatellite loci were isolated from genomic DNA derived
from a Propithecus tattersalli from the Daraina area in north-eastern Madagascar. Population genetic parameters were estimated on 20 individuals from two
Daraina forest fragments to determine the potential utility of this marker suite for future studies on the golden-crowned
sifaka. 相似文献