国产唇形科香茶菜属一新记录种——暗红香茶菜

报道了采自西藏错那县的唇形科香茶菜属的一个中国新记录种,即暗红香茶菜Isodon atroruber R. A. Clement。本研究对该种进行了描述,并应用扫描电子显微镜和光学显微镜对其叶表皮、花粉及小坚果的微形态特征进行了观察和测量。此外,还编制了西藏产19种香茶菜属植物的检索表。     

香茶菜属3种植物花粉形态的扫描电镜观察

应用扫描电子显微镜对香茶菜(Isodon amethystoides)、大萼香茶菜(I. macrocalyx)、显脉香茶菜(I. nervosa)8个居群的花粉进行了形态观察.结果表明,不同居群的香茶菜的花粉在形态上具有一定共同特性 ,但在外壁纹饰、大小、穴的形状上存在差异.香茶菜属不同种在花粉形态、大小、外壁纹饰、穴分布情况存在较明显的差异.这些花粉表面微观形态的差异可为品种鉴定提供依据.     

毛萼香茶菜二萜化合物的高效液相色谱定量分析

本文报道用HPLC分离测定毛萼香茶菜(Rabdosia eriocalyx)中的二萜化合物。总二萜提取物在zorbax ODS柱上,用甲醇-水(70:30)作流动相,毛萼晶乙、毛萼晶丙等五个化合物在18分钟内能很好分离。以odonicin为内标,用峰面积比测定各组份的含量。结果符合一般含量测定的要求,此法可作为香茶菜二萜化合物一类生物活性物质的分析及发现新二萜化合物的前导性有效筛选手段。     

国产唇形科香茶菜属一新记录种——暗红香茶菜（英文）

报道了采自西藏错那县的唇形科香茶菜属的一个中国新记录种,即暗红香茶菜Isodon atroruber R.A.Clement。本研究对该种进行了描述,并应用扫描电子显微镜和光学显微镜对其叶表皮、花粉及小坚果的微形态特征进行了观察和测量。此外,还编制了西藏产19种香茶菜属植物的检索表。     

甘肃香茶菜属药用植物资源分布及其利用对策

目的：调查统计甘肃省香茶菜属药用植物资源,为该属植物资源的合理开发利用提供科学依据。方法：野外调查、采集标本和查阅相关文献资料。结果：经初步研究,甘肃省有12种该属植物,记述了它们在省内的主要分布范围及药用价值。结论：甘肃东南部香茶菜属植物资源较为丰富,有待进一步研究,并提出了相应的开发保护措施。     

叶穗香茶菜的二萜成分

从云南省中甸县产叶穗香茶菜[Rabdosia phyllostachys(Diels)Hara]叶中分得1个新二萜化合物,命名为叶穗香茶菜乙素(phyllostachysin B),经各项波谱数据确定,叶穗香茶菜乙素为6β,11β,14β—三羟基-15α-乙酰氧基-对映-贝壳杉-16-烯-20 醛-7-酮。另一个为已知化合物叶穗香茶菜甲素(phyllostachysin A)。     

尾叶香茶菜不同部位挥发性成分比较分析北大核心CSCD

本文采用气流吹扫微萃取法对尾叶香茶菜根、茎、叶挥发性成分进行分析比较。采用GC-MS对挥发性成分进行分离检测,与标准谱库对比进行定性,采用面积归一化法计算各化合物的相对含量。尾叶香茶菜共检测到149个峰,与标准谱库比对,根、茎、叶分别鉴定出80、85、79种挥发性成分,占各部位挥发油总量的93. 66%、88. 05%和72. 62%。采用化学方法结合主成分分析法对尾叶香茶菜根、茎、叶的挥发性成分进行分析,结果显示根、茎、叶的挥发性成分在组成和含量上均存在一定差异,本研究为尾叶香茶菜的进一步开发利用提供参考依据。     

维西香茶菜的二萜成分

维西香茶菜Rabdosia weisiensis C. Y. Wu产云南西北部海拔2600米沟谷中,其化学成分的研究未见报道。从该植物叶的乙醚提取物中,分得两个二萜成分,一为已知成分trichorabdal A(2),一为新成分,命名为维西香茶菜甲素weisiensin A(1)。 维西香茶菜甲素weisiensin A(1),C_(26)H_(36)O_9,mp 298—300℃,其~(13)C NMR谱显示存在三个CH_3,三个CH_2,八个CH,三个四取代碳,三个Ac,二个烯碳和一个羰基     

香茶菜属一新种和一新变种

本文报道在广西发现的唇形科一新种和一新变种。     

A method for the selection of production media for actinomycete strains based on their metabolite HPLC profiles

The manipulation of growth conditions of microorganisms is a common strategy used by pharmaceutical companies to improve the quantities and spectra of secondary metabolites with potential therapeutic interest. In this work, the effects of fermentation media on secondary metabolite production from a set of Actinomycetes was statistically compared. For this purpose, we created an automated method for comparing the ability of microorganisms to produce different secondary metabolites. HPLC analyses guided the selection of those media in which a wider chemical diversity was obtained from microorganisms inoculated in a wide spectrum of production media. Fermented media yielding a better secondary metabolite profile were included in subsequent drug discovery screening.     

植物材料中尿囊素及尿囊酸的高效液相色谱法测定

尿囊酸性质极不稳定,需要有快速、准确的分离检测方法。本试验用高效液相色谱法分离检测尿囊素和尿囊酸,并认为该法是一种较好的测试方法。     

HPLC法测定红景天居群中红景天苷的分布式样

本文以HPLC法作为测定红景天苷的方法,调查红景天苷在红景天不同种、不同居群中的分布式样。实验中采集不同种、同种不同居群、同居群不同个体的红景天样品,进行红景天苷的含量测定。结果表明红景天苷在不同红景天中分布差异较大,同种不同居群间多型性、居群内及个体间多态性显著。HPLC比法简便、快速、准确、重复性好,适用于研究红景天苷在药用红景天居群中的分布。     

Biofilm formation by five species of Candida on three clinical materials

Most recalcitrant infections are associated with colonization and microbial biofilm development. These biofilms are difficult to eliminate by the immune response mechanisms and the current antimicrobial. Fungi can form biofilms on biomaterials commonly used in clinical practice (intravascular catheters, dentures, heart valves, implanted devices, contact lenses and other devices) and are associated with infections.A variety of in vitro models using different substrates/devices have been described. These models have been used to investigate the effect of different variables, including flow, growth time, nutrients and physiological conditions on fungal biofilm formation, morphology and architecture.The purpose of our study is to analyze biofilm formation capacity by 84 strains of Candida spp. (23 C. albicans, 23 C. parapsilosis, 16 C. tropicalis, 17 C. glabrata and 5 C. krusei) on three materials used in medical devices and its quantification using a method based on viable cell count.Under the conditions of our study, all assayed Candida strains have been able to form biofilms. All species showed greater biofilm formation capacity on Teflon™, with the exception of C. glabrata which displayed higher biofilm formation capacity on PVC. Biofilm formation by Candida spp. varies depending on the type of material on which it grows and on the species and strain of Candida.The method we propose could be of great use to deepen scientific knowledge on this subject of remarkable clinical significance, considering the absence of standard biofilm formation and quantification techniques on the catheters and the level of difficulty associated to those available.     

Identification of medicinal mushroom species based on nuclear large subunit rDNA sequences

The purpose of this study was to develop molecular identification method for medical mushrooms and their preparations based on the nucleotide sequences of nuclear large subunit (LSU) rDNA. Four specimens were collected of each of the three representative medicinal mushrooms used in Korea: Ganoderma lucidum, Coriolus versicolor, and Fomes fomentarius. Fungal material used in these experiments included two different mycelial cultures and two different fruiting bodies from wild or cultivated mushrooms. The genomic DNA of mushrooms were extracted and 3 nuclear LSU rDNA fragments were amplified: set 1 for the 1.1-kb DNA fragment in the upstream region, set 2 for the 1.2-kb fragment in the middle, and set 3 for the 1.3-kb fragment downstream. The amplified gene products of nuclear large subunit rDNA from 3 different mushrooms were cloned into E. coli vector and subjected to nucleotide sequence determination. The sequence thus determined revealed that the gene sequences of the same medicinal mushroom species were more than 99.48% homologous, and the consensus sequences of 3 different medicinal mushrooms were more than 97.80% homologous. Restriction analysis revealed no useful restriction sites for 6-bp recognition enzymes for distinguishing the 3 sequences from one another, but some distinctive restriction patterns were recognized by the 4-bp recognition enzymes AccII and HhaI. This analysis was also confirmed by PCR-RFLP experiments on medicinal mushrooms.     

Determination of aflatoxins in medicinal herbs by HPLC. An efficient method for routine analysis

A fast and easy to perform method for the routine determination of aflatoxins in medicinal herbs was developed. The described method involves a single-step extraction with a non-chlorinated solvent, an immunoaffinity clean-up and HPLC with fluorescence detection. Whilst assays with naturally contaminated and with spiked samples of several herbs showed that the recoveries were somewhat low and dependent on the kind of sample and the degree of grinding, the intra-batch reproducibility was good, allowing a reliable quantitation by the standard-addition method. Good linearity, repeatability and accuracy were demonstrated in assays involving several medicinal herbs. The limit of quantitation was of the order of 0.05-0.1 ng/g, being dependent of the species analysed, and the method required no tedious concentration or back-extraction steps.