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1.
The bacterial strain M1T8B10 T was isolated from cow dung in Suwon, Republic of Korea. The strain was a Gram stain-positive rod, nonmotile, and non-spore-forming.
According to 16S rRNA gene sequence analysis, the strain fell within the clade of the genus Leucobacter, showing the highest sequence similarities with Leucobacter aridicollis L-9 T (98.7%), Leucobacter iarius 40 T (98.4%), and Leucobacter komagatae JCM 9414 T (98.2%). Cell-wall peptidoglycan contained the diagnostic diamino acid 2,4-diaminobutyric acid of the genus Leucobacter, showing B-type cross-linked peptidoglycans. The major fatty acids were anteiso-C 15:0, iso-C 16:0, and anteiso-C 17:0. The quinone system consisted of the menaquinones MK-11 (78%) and MK-10 (22%). The polar lipid profiles contained diphosphatidylglycerol,
phosphatidylglycerol, and an unidentified glycolipid. Differences in several physiological features including nitrate reduction
enabled the isolate to be differentiated from all recognized Leucobacter species. Based on these phylogenetic, chemotaxonomic, and phenotypic results, the isolate represents a novel species, for
which the name Leucobacter denitrificans sp. nov. is proposed. The type strain is M1T8B10 T (=KACC 14055 T =NBRC 106309 T). 相似文献
2.
A Gram-positive aerobic rod-shaped non-motile bacterium designated A23 T was isolated from bamboo extract that had been used to remove odor and was characterized to determine its taxonomic position.
16S rRNA gene sequence analysis revealed that strain A23 T belongs to the phylum Actinobacteria. The highest degree of sequence similarities was determined to be with Leucobacter salsicius M1-8 T (96.7%), Leucobacter exalbidus K-540B T (96.4%), Leucobacter chromiireducens subsp. chromiireducens L-1 T (96.4%), Leucobacter komagatae IFO 15245 T (96.4%) and Leucobacter aerolatus Sj10 T (96.4%). Chemotaxonomic data revealed that strain A23 T possesses menaquinone MK11, and its cell wall peptidoglycan contained 2,4-diaminobutyric acid, alanine, glycine, glutamic
acid and γ-aminobutyric acid. The polar lipid profile of strain A23 T contained diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The predominant fatty acids were iso-C 16:0 (31.5%), anteiso-C 15:0 (43.2%) and anteiso-C 17:0 (13.9%), all of which corroborated the assignment of the strain to the genus Leucobacter. Based on these data, A23 T (=KEMC 551-022 T = JCM 17538 T) should be classified as the type strain for a novel Leucobacter species, for which the name Leucobacter margaritiformis sp. nov. is proposed. 相似文献
3.
A phosphate-solubilizing bacterial strain designated PS38 T was isolated from farm soil. The isolate was a Gram-positive, motile, endospore-forming, rod-shaped bacterium. It grew optimally
at 37°C and pH 7.5. The predominant cellular fatty acids were anteiso-C 15:0, anteiso-C 17:0, and iso-C 16:0. The DNA G+C content was 49.5 mol% and the predominant menaquinone was MK-7. Phylogenese analyses based on 16S rRNA gene
sequences showed that the strain PS38 T belonged to the genus Paenibacillus and was most closely related to Paenibacillus chibensis JCM 9905 T, P. barengoltzii SAFN-016 T, P. timonensis 2301032 T, and P. motobuensis MC10 T with 96.3%, 96.0%, 95.9%, and 95.5% 16S rRNA gene sequence similarity, respectively. On the basis of morphological, chemotaxonomic,
physiological, and phylogenetic properties, strain PS38 T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus telluris sp. nov. is proposed. The type strain is PS38 T (=KCTC 13946 T =CGMCC 1.10695 T). 相似文献
4.
A novel Gram-positive, halotolerant, non-sporulating, non-motile, catalase-positive, oxidase-negative and aerobic bacterium,
designated strain JSM 078085 T, was isolated from sea water collected from the South China Sea. Strain JSM 078085 T exhibited a rod-coccus growth cycle and produced a yellow pigment. The strain was able to grow in the presence of 0–12% (w/v)
NaCl and at pH 6.0–9.5 and 4–35°C; optimum growth was observed at pH 7.0 and 25–30°C in the absence of NaCl. The peptidoglycan
type was A4 α ( l-Lys– l-Ala– l-Glu). Cell-wall sugars contained galactose and glucose. Strain JSM 078085 T contained menaquinone MK-9(H 2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major
polar lipids. The major cellular fatty acids were anteiso-C 15:0, iso-C 15:0 and anteiso-C 17:0 and the DNA G + C content was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that
strain JSM 078085 T should be assigned to the genus Arthrobacter, being most closely related to the type strain of Arthrobacter rhombi (sequence similarity 97.1%), and the two strains formed a distinct lineage in the phylogenetic tree. The level of DNA–DNA
relatedness between strain JSM 078085 T and the type strain of Arthrobacter rhombi was 10.6%. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data
supported the view that strain JSM 078085 T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter halodurans sp. nov. is proposed. The type strain is JSM 078085 T (=DSM 21081 T=KCTC 19430 T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 078085 T is EU583729. 相似文献
5.
A Gram-positive, aerobic, non-motile actinobacterial strain, designated YIM C01235 T, was isolated from a soil sample collected from the Swallow Cave, Yunnan province, south-west China. The isolate grew at 10–30 °C, pH 6.0–9.0 and 0–8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest similarity to Saccharopolyspora gloriosae YIM 60513 T (96.8 %), and lower 16S rRNA gene sequence similarities (95.1–96.7 %) with the other species of the genus Saccharopolyspora. The whole-cell hydrolysates contained meso-diaminopimelic acid ( meso-DAP), arabinose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unknown glycolipids, two unknown phospholipids and one polar lipid. MK-9(H 4) was the predominant menaquinone. The major fatty acids were iso-C 15:0, iso-C 16:0, anteiso-C 15:0 and anteiso-C 17:0. Mycolic acids were not detected. The genomic DNA G+C content was 69.1 mol%. These chemotaxonomic data, together with its morphological properties, were consistent with the assignment of strain YIM C01235 T to the genus Saccharopolyspora. The results of physiological and biochemical tests allowed strain YIM C01235 T to be differentiated phenotypically from all the recognized Saccharopolyspora species. On the basis of evidence from this polyphasic study, the novel species Saccharopolyspora cavernae sp. nov. is proposed. The type strain is YIM C01235 T (=DSM 45825 T = CCTCC AA 2012022 T). 相似文献
6.
A Gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated SC-N012 T, was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. The strain exhibited
phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Sequence analysis of the 16S rRNA gene of SC-N012 T revealed the closest match (98.9% pair wise similarity) with Bacillus clausii DSM 8716 T. However, DNA–DNA hybridization experiments indicated low levels of genomic relatedness (32%) with this strain. The major
components of the fatty acid profile are iso-C 15:0, anteiso-C 15:0, iso-C 17:0 and anteiso-C 17:0. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The G+C content of the genomic DNA is 43.0 mol%. The lipids present in strain SC-N012 T are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and two unknown phospholipids. Their predominant
respiratory quinone was MK-7. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and
phylogenetic data based on 16S rRNA gene sequencing allowed strain SC-N012 T to be described as members of novel species of the genus Bacillus, for which the name Bacillus rhizosphaerae sp. nov. is proposed. The type strain is SC-N012 T (=DSM 21911 T = NCCB 100267 T). 相似文献
7.
A bacterial strain, designated AETb3-4T was isolated from the rhizosphere of lily. Comparison of 16S rRNA gene sequences showed that the sequence from strain AETb3-4T exhibits high sequence similarity with those of Arthrobacter silviterrae KIS14-16T (97.9%), Arthrobacter livingstonensis LI2T (97.2%) and Arthrobacter stackebrandtii CCM 2783T (97.0%). Whole genome average nucleotide identity (ANI) and the digital DNA-DNA hybridization (dDDH) values between strain AETb3-4T and the reference strains A. silviterrae DSM 27180T, A. livingstonensis L12T and A. stackebrandtii DSM 16005T were below 83.6% and 27.7%, respectively, values which are considerably below the proposed thresholds for the species delineation, consistent with the proposal that strain AETb3-4T represents a novel species. The genome size of strain AETb3-4T is 4.33 Mb and the genomic DNA G?+?C content is 67.3%. The main polar lipids were identified as phosphatidylglycerol, diphosphatidylglycero, phosphatidylinositol and an unidentified glycolipid. The major fatty acids (>?10%) were identified as anteiso-C15: 0 and anteiso-C17: 0. The predominant menaquinone was found to be menaquinone 9 (MK-9) (H2) (82.2%). Phenotypic tests allowed the strain to be differentiated from its close phylogenetic neighbors. Based on the results obtained, it is proposed that the strain AETb3-4T (=?CFCC 16390T?=?LMG 31708T) represents a novel species in the genus Arthrobacter, for which the names Arthrobacter wenxiniae sp. nov. is proposed. In addition, the novel strain AETb3-4T has multiple plant growth-promoting characters including ACC-deaminase activity and production of IAA. Furthermore, the genome contains secondary metabolite biosynthesis gene clusters, including a carotenoid biosynthetic gene cluster, suggesting potential capacities for secondary metabolite synthesis. These data suggest that strain AETb3-4T may have potential applications both in medicine and sustainable agriculture. 相似文献
8.
A moderate halophile, strain X50 T, was isolated from mustard kimchi, a traditional Korean fermented food. The organism grew under conditions ranging from 0–15.0%
(w/v) NaCl (optimum: 3.0%), pH 7.0–10.0 (optimum: pH 9.0) and 15–45°C (optimum: 37°C). The morphological, physiological, and
biochemical features and the 16S rRNA gene sequences of strain X50 T were characterized. Colonies of the isolate were creamcolored and the cells were rod-shaped. Phylogenetic analysis based
on the 16S rRNA gene sequence indicated that strain X50 T belongs to the genus Oceanobacillus and is closely related phylogenetically to the type strain O. iheyensis HTE831 T (98.9%) and O. oncorhynchi subsp. oncorhynchi R-2 T (97.0%). The cellular fatty acid profiles predominately included anteiso-C 15:0 and iso-C 15:0. The G+C content of the genomic DNA of the isolate was 37.9 mol% and the major isoprenoid quinone was MK-7. Analysis of the
16S rRNA gene sequences, DNA-DNA relatedness and physiological and biochemical tests indicated genotypic and phenotypic differences
among strain X50 T and reference species in the genus Oceanobacillus. Therefore, strain X50 T was proposed as a novel species and named Oceanobacillus kimchii. The type strain of the new species is X50 T (=JCM 16803 T =KACC 14914 T =DSM 23341 T). 相似文献
9.
A novel Gram-stain-negative, motile by means of gliding, and short rod-shaped bacterium, designated HS916T, was isolated from soil polluted by sewer water in Cheonan-si, South Korea. Growth occurred at 10–35°C (optimum 30°C), pH 6.0–8.0 (optimum pH 7.0), and 0–1% sodium chloride (NaCl, w/v). Based on similarities of 16S rRNA gene sequences, strain HS916 T was closely related to members of the genus Flavobacterium, exhibiting the highest sequence similarities with Flavobacterium glycines Gm-149 T (96.4%), followed by F. granuli Kw05 T (96.3%), F. fluminis 3R17 T (96.3%), F. aquicola TMd3a3 T (96.2%), and F. nitratireducens N1 T (96.2%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HS916T was placed in a monophyletic cluster with F. nitratireducens N1 T and F. fluminis 3R17 T. The predominant fatty acids (> 5% of the total) of strain HS916 T were iso-C 15:0, anteiso-C 15:0, iso-C 15:0 3-OH, C 17:1ω6 с, C 16:0 3-OH, iso-C 17:0 3-OH, and summed feature 3 (C 16:1ω7 с and/or C 16:1ω6 с). The major polar lipids of the strain comprised phosphatidylethanolamine, unidentified aminolipids, and five unidentified lipids. The predominant respiratory quinone and the major polyamine were menaquinone-6 (MK-6) and symhomospermidine, respectively. The DNA G + C content of strain HS916 T was 34.9 mol%. Based on polyphasic analyses, strain HS916 T represents a novel species belonging to the genus Flavobacterium, for which the name Flavobacterium parvum sp. nov. is proposed. The type strain is HS916T (= KACC 19448 T = JCM 32368 T). 相似文献
10.
A strain of Streptomyces, MBRL 179 T, isolated from a sample from a Limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain formed a monophyletic clade with Streptomyces spinoverrucosus NBRC 14228 T (16S rRNA gene sequence similarity of 99.3 %) in the Neighbour-joining tree. DNA–DNA hybridization experiment gave a DNA–DNA relatedness value of 34.7 % between MBRL 179 T and S. spinoverrucosus NBRC 14228 T. Strain MBRL 179 T contained LL-diaminopimelic acid, xylose, glucose, and mannose in the whole cell-wall hydrolysates along with small amount of ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, with other unknown phospholipids and aminophospholipid. MK-9(H 6), MK-9(H 8) and MK-9(H 4) were the predominant menaquinones detected. The major fatty acids were anteiso-C 16:0 (28.1 %), iso-C 16:0 (20.3 %), C 16:0 (9.4 %) and anteiso-C 17:0 (8.3 %). The G+C content of the genomic DNA was 71.1 %. Based on the polyphasic experiment results, the strain MBRL 179 T merits recognition as a representative of a novel species of the genus Streptomyces for which the name Streptomyces muensis sp. nov. is proposed; the type strain is MBRL 179 T (=JCM 17576 T = KCTC 29124 T). 相似文献
11.
A novel actinomycete strain, designated YIM 45720 T, was isolated from a Cephalotaxus fortunei rhizophere soil sample collected from Yunnan Province, southwest China. The strain formed well-differentiated aerial and
substrate mycelia. Chemotaxonomically, it contained LL-diaminopimelic acid in the cell wall. The cell-wall sugars contained
ribose, mannose, and galactose with traces of glucose and xylose. Phospholipids were diphosphatidylglycerol, phosphatidylethanolamine,
phosphatidylcholine and phosphatidylinositol. MK-9 (H 8) was the predominant menaquinone. The major fatty acids (>10%) were iso-C 16:0, iso-C 15:1 and anteiso-C 15:0. The G + C content of the DNA was 70 mol%. Phylogenetic analysis data based on 16S rRNA gene sequence showed that strain YIM 45720 T formed a distinct branch with the type strain of Streptomyces scabrisporus JCM 11712 T within the genus Streptomyces. On the basis of the phenotypic and genotypic characteristics, strain YIM 45720 T (=DSM 41883 T = CCTCC AA 206006 T) is proposed as the type strain of a novel species, Streptomyces serianimatus sp. nov. 相似文献
12.
A novel yellow pigmented, Gram-positive, aerobic and heavy metal biosorptive bacterium designated SYP-B2667 T was isolated from rhizosphere soil of Epilobium hirsutum L. in Tongren, Guizhou province, China. Based on 16S rRNA gene sequence analyses, it was shown that strain SYP-B2667 T represents a novel species in the genus Leucobacter, with Leucobacter chromiireducens subsp. solipictus JCM 15573 T as a close phylogenetic neighbour (sequence similarity of 98.2%). Chemotaxonomic characteristics also supported the affiliation to the genus Leucobacter. Strain SYP-B2667 T was determined to have a DNA G+C content of 66.6 mol%; 2,4-diaminobutyric acid in the cell wall peptidoglycan amino acids; MK-11 as predominant menaquinone; an abundance of anteiso-C 15:0 and anteiso-C 17:0 fatty acids; and polar lipids including diphosphatidylglycerol, phosphatidylglycerol, glycolipids and unidentified phospholipids. The DNA–DNA hybridization value between strain SYP-B2667 T and L. chromiireducens subsp. solipictus JCM 15573 T was 19.7?±?2.8%. Based on these phylogenetic and phenotypic results, it can be concluded that strain SYP-B2667 T represents a novel species, for which the name Leucobacter epilobiisoli sp. nov. is proposed. The type strain is SYP-B2667 T (=DSM 105145 T=CPCC 204976 T). This strain can tolerate and adsorb five heavy metals and so may have potential to facilitate heavy metal removal and bioremediation. 相似文献
13.
A Streptomyces-like actinomycete strain, designated as YIM 78087 T, was isolated from a sediment sample collected from Hehua hot spring in Tengchong, Yunnan province, south-west China. The taxonomic position of strain YIM 78087 T was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 78087 T belongs to the genus Streptomyces and is closely related to Streptomyces fimbriatus DSM 40942 T, Streptomyces marinus DSM 41968 T and Streptomyces qinglanensis DSM 42035 T (97.18, 97.05 and 97.1 % similarity, respectively). Combined with the low values of DNA–DNA hybridization between strain YIM 78087 T and its closest neighbours, these analyses indicated that this new isolate represents a different genomic species in the genus Streptomyces. The predominant menaquinones of strain YIM 78087 T were identified as MK-9 (H 4) and MK-9 (H 6). The major fatty acids were identified as anteiso-C 15:0 (28.4 %), anteiso-C 17:0 (23.0 %) and iso-C 16:0 (15.1 %). The whole-cell hydrolysates found to contain glucose, mannose and ribose. The DNA G+C content was determined to be 73.0 mol%. Based on the comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 78087 T represents a novel species of the genus Streptomyces, for which the name Streptomyces calidiresistens sp. nov., is proposed. The type strain is YIM 78087 T (=BCRC 16955 T=DSM 42108 T=JCM 19629 T). 相似文献
14.
A Gram-positive and endospore-forming bacterial strain, designated S4 T, was isolated from the rhizosphere of ferns in Daejeon, Republic of Korea. This isolate is strictly aerobic, motile, and
rod-like in shape, and it is positive for catalase, oxidase, esterase lipase, and β-galactosidase activities. In addition,
this strain grows when cultured at temperatures between 15 and 37°C and at pH values ranging from 5.5 to 9.0. The DNA G+C
content was determined to be 53.2 mol%. Strain S4 T has meso-diaminopimelic acid in the cell-wall peptidoglycan; it also contains menaquinone 7 (MK-7) as the predominant isoprenoid quinone
and anteiso-C 15:0 (57.5%), iso-C 16:0 (11.3%), and C 16:0 (9.4%) as the major cellular fatty acids. Phylogenetic analysis based on alignments of the 16S rRNA gene sequence showed
that S4 T is affiliated with a cluster of strains within the genus Paenibacillaceae and is most closely related to Paenibacillus chinjuensis WN9 T, with 96.8% similarity. Based on the phylogenetic and phenotypic characteristics of strain S4 T, we believe that this isolate should be distinguished from all type species of the genus Paenibacillus and should thus represent a novel taxon within the genus Paenibacillus. We propose naming this type species Paenibacillus filicis sp. nov. for the rhizosphere isolate; the type strain will be known as S4 T (=KCTC 13693 T =KACC 14197 T =JCM 16417 T). 相似文献
15.
A Gram-negative, coccoid shaped bacterium isolated from the outer surface of the medicinal leech Hirudo medicinalis was characterized. The 16S rRNA gene sequence comparison revealed that the bacterium was closely related to species of the genus Luteolibacter. Luteolibacter pohnpeiensis was the most closely related species (94.6 % sequence similarity), followed by Luteolibacter luojiensis (93.4 %) and Luteolibacter algae (93.3 %). Chemotaxonomic data (major ubiquinone: MK-9; major polar lipids: phosphatidylethanolamine and phosphatidylglycerol; and major fatty acids: iso-C 14:0, C 16:0, iso-C 16:1, and anteiso-C 15:0) supported the affiliation of the isolate to the genus Luteolibacter. DNA–DNA hybridizations with the type strain of L. pohnpeiensis was 31 % (reciprocal value 30 %). A phenotypic differentiation of strain E100 T from L. pohnpeiensis and the other Luteolibacter species was possible by several physiological tests. We conclude Strain E100 T represents a novel species, for which we propose the name Luteolibacter cuticulihirudinis sp. nov. with the type strain E100 T (=CCM 8400 T = LMG 26924 T). 相似文献
16.
Strain AK12 T, an orange pigmented Gram-negative, rod shaped, non-motile bacterium, was isolated from a mud sample collected from a brackish
water pond at Rampur of West Bengal, India. The strain was positive for oxidase, catalase and phosphatase. The predominant
fatty acids were iso-C 15:0 (42.7%), iso-C 17:0 3OH (13.2%), C 16:1ω7c/C 16:1ω6c (summed feature 3) (8.0%), iso-C 17:1 I/anteiso-C 17:1 B (summed feature 4) (6.1%) and iso-C 17:1ω9c/C 16:0 10-methyl (summed feature 9) (9.4%). Strain AK12 T contained MK-7 as the major respiratory quinone and phosphatidylethanolamine, one unidentified aminophospholipid and six
unidentified lipids as the polar lipids. The G + C content of DNA of the strain AK12 T was 46.2 mol%. The 16S rRNA gene sequence analysis indicated that strain AK12 T was member of the genus Echinicola and closely related to Echinicola vietnamensis, Echinicola pacifica and Echinicola jeungdonensis with pair-wise sequence similarity of 96.8, 96.3 and 96.0% respectively. Phylogenetic analyses indicated that the strain
AK12 T clustered with E. vietnamensis and together with E. pacifica and E. jeungdonensis with a phylogenetic distance of 5.1, 6.3 and 6.6% (94.9, 93.7 and 93.4% similarity) respectively. Based on data from the
current polyphasic study, strain AK12 T is proposed as a novel species of the genus Echinicola, for which the name Echinicola shivajiensis sp. nov. is proposed. The type strain of E. shivajiensis is AK12 T (= MTCC 11083 T = JCM 17847 T). 相似文献
17.
A novel aerobic, Gram-positive, non-motile halotolerant actinobacterium, designated strain YIM 96448 T, was isolated from a saline soil sample collected from the south bank of Aiding Lake in Xinjiang Province, northwest China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 96448 T is closely related to Phytoactinopolyspora endophytica EGI 60009 T (98.0%) and Phytoactinopolyspora alkaliphila EGI 80629 T (97.0%). Optimum growth of the strain was observed at 28-37 °C, pH 7–9 and in the presence of 5–8% NaCl (w/v). The respiratory quinone of strain YIM 96448 T was determined to be MK-9(H 4). The major fatty acids (>10%) were identified as anteiso-C 15:0, iso-C 16:0, anteiso-C 17:0 and summed feature 4 (iso-C 17:1 I and/or anteiso-C 17:1B). The polar lipid profile was found to consist of diphosphatidylglycerol, an unidentified aminophospholipid, phosphatidylglycerol, two phosphatidylinositol mannosides, three unidentified phosphoglycolipids and four unidentified phospholipids. The DNA G+C content was determined to be 68.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain YIM 96448 T should be classified as a novel species in the genus Phytoactinopolyspora, for which the name Phytoactinopolyspora halotolerans sp. nov. is proposed. The type strain is YIM 96448 T (=CCTCC AB 2017055 T = KCTC 39924 T). 相似文献
18.
A Gram-negative, aerobic, rod-shaped, and red-pigmented bacterial strain, HMC5104 T, was isolated from a solar saltern, found in Jeungdo, Republic of Korea (34°59′47″N 126°10′02″E). The major fatty acids were
summed feature 4 (comprising iso-C 17:1 I and/or anteiso-C 17:1 B; 37.2%), iso-C 15:0 (20.4%), and iso-C 17.0 30H (15.3%). The DNA G+C content was 46.0 mol%. The major isoprenoid quinone was menaquinone-7 (MK-7). A phylogenetic tree
based on 16S rRNA gene sequences showed that strain HMC5104 T formed a lineage within the genus Pontibacter, and was closely related to Pontibacter korlensis (95.9%), P. roseus (94.9%), and P. actiniarum (94.3%). Similarities to all other Pontibacter species were between 95.9–93.9%. On the basis of the evidence presented in this study, strain HMC5104 T represents a novel species of the genus Pontibacter, for which the name Pontibacter salisaro sp. nov. is proposed. The type strain is HMC5104 T (=KCTC 22712 T = NBRC 105731 T). 相似文献
19.
A Gram-positive, moderately halophilic, endospore-forming, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium,
designated strain JSM 089168 T, was isolated from saline soil collected from Naozhou Island, Leizhou Bay, South China Sea. The organism was able to grow
with 2–25% (w/v) total salts (optimum, 5–10%), at pH 6.0–10.0 (optimum, pH 8.0) and 10–45°C (optimum, 30°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The strain contained MK-7 as the predominant menaquinone,
and diphosphatidylglycerol and phosphatidylglycerol as the major polar lipids. The major cellular fatty acids were anteiso-C 15:0, iso-C 15:0 and anteiso-C 17:0, and the DNA G + C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that
strain JSM 089168 T should be assigned to the genus Virgibacillus, being related most closely to the type strains of Virgibacillus carmonensis (sequence similarity 97.6%), Virgibacillus necropolis (97.3%) and Virgibacillus halodenitrificans (97.1%). Levels of DNA–DNA relatedness between strain JSM 089168 T and the type strains of V. carmonensis, V. necropolis and V. halodenitrificans were 20.4, 14.3 and 12.0%, respectively. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics
and chemotaxonomic data supported the view that strain JSM 089168 T represents a novel species of the genus Virgibacillus, for which the name Virgibacillus litoralis sp. nov. is proposed. The type strain is JSM 089168 T (=DSM 21085 T =KCTC 13228 T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 089168 T is FJ425909. 相似文献
20.
A novel actinomycete strain, designated TRM 40133 T, was isolated from a hypersaline habitat of Tarim basin in Xinjiang Province, north-west China. Its taxonomic status was
determined using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain
showed that it formed a well-seperated sub-branch within the radiation of the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity was found between the strain TRM 40133 T and Saccharopolyspora qijiaojingensis YIM 91168 T (96.5%). The chemotaxonomic characteristics of the isolate are typical for the genus Saccharopolyspora. It contained meso-DAP as the diagnostic diamino acid. Whole cell hydrolysate contained arabinose, xylose, ribose and glucose. The diagnostic
phospholipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. The main
menaquinone was MK-9(H 6) and MK-9(H 4). No mycolic acid was detected. The predominant cellular fatty acids were iso-C 16:0 and anteiso-C 17:0. The G+C content of the genomic DNA was 68.2 mol%. In addition, the strain TRM 40133 T had a phenotypic profile that readily distinguished it from the recognized representatives of the genus Saccharopolyspora. The strain TRM 40133 T therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora lacisalsi sp. nov. is proposed. The type strain is TRM 40133 T (=KCTC 19987 T =CCTCC AA 2010012 T). 相似文献
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