A sustainable sensitive spectrofluorimetric approach for the determination of the multipotent protein lactoferrin in different pharmaceuticals and infant milk formula: Compliance with greenness metrics

The current study presents the first spectrofluorimetric approach for the estimation of lactoferrin, depending on the measurement of its native fluorescence at 337 nm after excitation at 230 nm, without the need for any hazardous chemicals or reagents. It was found that the fluorescence intensity versus concentration calibration plot was linear over the concentration range of 0.1–10.0 μg/mL with quantitation and detection limits of 0.082 and 0.027 μg/mL, respectively. The method was accordingly validated according to the ICH recommendations. The developed method was applied for the estimation of lactoferrin in different dosage forms, including capsules and sachets with high percent recoveries (97.84–102.53) and low %RSD values (<1.95). Lactoferrin is one of the key nutrients in milk powder and a significant nutritional fortifier. In order to assess the quality of milk powder, it is essential to rapidly and accurately quantify the lactoferrin content of the product. Therefore, the presented study was successfully applied for the selective estimation of lactoferrin in milk powder with acceptable percent recoveries (96.45–104.92) and %RSD values (≤3.607). Finally, the green profile of the method was estimated using two assessment tools: Green Analytical Procedure Index (GAPI) and Analytical GREEnness (AGREE), which demonstrated its excellent greenness.     

A novel sustainable second-derivative synchronous spectrofluorimetric method for the simultaneous determination of olmesartan medoxomil and rosuvastatin calcium in bulk and formulations: Integrated greenness and whiteness evaluation

The present research has established a quick and highly sensitive second-derivative synchronous fluorometric technique for the simultaneous quantification of a binary mixture of olmesartan medoxomil and rosuvastatin calcium. Simultaneously, the suggested approach was used to detect the synchronous fluorescence intensity of the cited drugs at Δ λ = 80 nm in ethanol to determine the concentrations of olmesartan medoxomil and rosuvastatin calcium at 265 and 240 nm, respectively. Various experimental conditions were tested, and each variable was analyzed and optimized. The calibration graphs were shown to be linear within ranges of 0.1–2.0 and 0.5–6.0 μg ml⁻¹ for each drug concentration, respectively. The newly developed Green Solvents Selecting Tool (GSST) was utilized to assess the solvent's sustainability. Furthermore, the proposed method was found to be environmentally friendly after being evaluated with three different tools [the Green Analytical Procedure Index (GAPI), the Analytical Greenness Metric (AGREE), and the Analytical Eco-Scale with Eco-score equal to 95]. The whiteness qualities were also studied using the Red–Green–Blue (RGB12) model, which was recently designed and showed a high score equal to 92.9. The proposed method’s good findings, as well as its ongoing sustainability, simplicity, and economy, stimulate its application in QC laboratories.     

Spider diagram with greenness evaluation metrics for assessing the new synchronous spectrofluorimetric determination of bicalutamide and resveratrol in human plasma

A simple, selective, and eco-friendly synchronous fluorescence approach was introduced for the first time for the concurrent estimation of the anticancer combination therapy of bicalutamide and resveratrol. The method relies on measuring the synchronous fluorescence spectra of bicalutamide and resveratrol at 269 and 320 nm, respectively, using Δλ of 60 nm with ethanol as a green diluting solvent. The procedure was optimized, and the method was then fully validated. Excellent linearity (R² > 0.999) with very low detection limits (0.044 and 2.001 ng/ml) were obtained for both drugs, allowing for their analysis in human plasma. The green profile of the suggested approach was evaluated using the green solvents selecting tool (GSST), spider diagram for greenness index assessment, green analytical process index (GAPI), and Analytical GREEnness (AGREE) metric tools. These assessment metrics confirmed that the developed approach met the maximum number of green requirements, recommending its application as a green substitute for the regular analysis of the concerned drugs in human plasma. The simplicity of sample measurement enables and substantially accelerates the analysis, resulting in lower costs, enhanced procedure accuracy, and lower environmental effect.     

Method validation for the determination of ochratoxin A in green and soluble coffee by immunoaffinity column cleanup and liquid chromatography

A method was validated for the determination of ochratoxin A (OTA) in soluble and green coffee. Performance parameters evaluated included selectivity, accuracy, intermediate precision, linearity, limit of detection, limit of quantitation, and ruggedness. The method was found to be selective for OTA in both matrices tested. Recovery rates from soluble coffee samples ranged from 73.5 to 91.2%, and from green coffee samples from 68.7 to 84.5%. The intermediate precision (RSDr) was between 9.1 and 9.4% for soluble coffee and between 14.3 and 15.5% for green coffee analysis. The linearity of the standard calibration curve (r²) was <0.999 for OTA levels of 1.0–20.0 μg/kg in coffee samples. The limit of detection was determined to be 0.01 ng of OTA on column, while the limit of quantitation was found to be 0.03 ng on column. The limit of quantitation is equivalent to 0.6 μg/kg in soluble coffee samples and 0.3 μg/kg in green coffee samples. The results of the ruggedness trial showed two factors are critical for soluble coffee analysis: the extraction method, and the flow rate of the mobile phase. For green coffee analysis two critical factors detected were the extraction method and the storage temperature of the immunoaffinity column. Five samples of soluble coffee and 42 of green coffee were analysed using the validated method. All soluble coffee samples contained OTA at levels that ranged from 8.4 to 13.9 μg/kg. Six of the 42 green coffee samples analysed (14.3%) contained OTA at levels ranging from 0.9 to 19.4 μg/kg. The validated method can be used to monitor OTA levels in Colombian coffee for export or for local consumption.     

A diagnostic model for green productivity assessment of manufacturing processes

Goal, Scope and Background  Green Productivity (GP) is a new paradigm in sustainable manufacturing where resource conservation and waste minimization constitute the strategy in simultaneously enhancing environmental performance and productivity. This productivity approach to the sustainability of industries requires the adoption of clean production technology and the development of appropriate indicators and instruments to measure environmental performance in a continuous improvement strategy that focuses on the manufacturing stage of the product life cycle. The analysis may be expanded to include the entire life cycle with increasing details on impacts, improvement strategies and indicators. Methods  The study proposes a methodology for GP assessment that integrates the essential components of life cycle assessment (LCA) and multicriteria decision analysis specifically the analytic hierarchy process (AHP). LCA provides a systematic and holistic perspective for GP analysis that spans inventory, impact and improvement assessment. The AHP is utilized as a decision framework and valuation tool for impact and improvement assessment to come up with priority weights. Indicators are derived and measured from a streamlined LCA focused on a number of parameters within the gate-to-gate analysis to demonstrate the GP concept in relation to resource utilization and waste minimization. An input-output approach using a suitable material balance in a scenario analysis provides the basis of GP performance measurement. Results and Conclusion  The diagnostic model is applied on a semiconductor assembly/packaging operation. From the streamlined life cycle inventory, impact factors were derived for water resource depletion (WRD), energy resource depletion (ERD), human toxicity-air (HTA), human toxicity-land (HTL), human toxicity-water (HTW), aquatic ecotoxicity (ETA) and terrestrial ecotoxicity (ETT). Valuation of impact factors using the AHP showed the high significance of ETT, HTL, WRD and ERD. This especially reflects the impact of the industry on the solid waste problem as a result of emissions to land associated with human toxicity and ecotoxicity effects and the intensive use of water and energy resources. Using scenario analysis, the effect of implementing a process-based improvement technique on a product-specific operation was determined and the highest values in GP are for energy utilization, water utilization and terrestrial ecotoxicity. Recommendation and Perspective  Expert system technology was explored in developing a diagnostic prototype that emulates how human experts diagnose green productivity of manufacturing processes. The aim was to investigate how such a diagnosis could be performed in an intelligent fashion that it is also easily accessible as a decision support for industries. The expert system model will provide flexibility in testing the relationships of environmental performance and productivity parameters as well as in preserving and disseminating valuable human expertise in GP program implementation. This is a continuing research effort that is building the knowledge base for GP assessment. It will include case studies over a wider range or level of detail regarding the impacts and improvement techniques and the other stages of the product life cycle.     

Sensitive spectrofluorimetric determination of the prokinetic drug prucalopride succinate based on supramolecular aggregation approach with evaluation of method greenness: application to content uniformity test

The prokinetic drug, prucalopride (PCP) succinate, was determined using a new spectrofluorimetric approach with a highly sensitive, rapid, and simple procedure. The method exploited the enhancement of the inherent native fluorescence of PCP by micellar aggregation with sodium lauryl sulfate (SLS) as an anionic surfactant. Different factors that could affect the fluorescence intensity were carefully studied in order to achieve the maximal fluorescence signal. Measurement of the enhanced fluorescence was done at 354 nm after the excitation at 276 nm. The fluorescence intensity–concentration plot was rectilinear in the concentration range of 50–600 ng/ml with detection and quantitation limits of 13.9 and 42.1 ng/ml, respectively. The method underwent validation according to the International Council for Harmonisation criteria in order to assess its analytical performance, and promising results were achieved that proved the validity and reliability of the method. Furthermore, the method was employed effectively for the analysis of the cited drug in commercial pharmaceutical tablets.     

Integrating cost-benefits analysis and life cycle assessment of green roofs: a case study in Florida

Abstract

Multiple environmental benefits can be achieved by using a green roof instead of conventional roofs. To better understand the LCA and cost-effectiveness of a green roof, a case study was performed. Two energy models, one with conventional white roof and the other with green roof, were created using eQUEST software to compare the influence of green roof on building energy consumption. The results indicated that the application of a green roof reduced annual space heating and cooling electricity consumption by 9500 kWh (2.2 kWh per square meter). The LCA shows that by using an extensive green roof in lieu of a conventional white roof the LCA measures at the product, construction, and end-of-life stages increased due to the use of additional layers. However, these increases are offset by the reduction of LCA measures at the use stage such that the overall environmental impacts of green roof is less than that of conventional roof. To find out the cost-effectiveness of green roof, a 50-year cost-benefits analysis was conducted. The analysis showed that the net savings of the green roof is negative compared to the white roof it replaced due to its higher initial cost and follow on maintenance cost.     

醴陵市绿色屋顶生态系统服务潜力评估及规划

近年来,生态系统服务评估逐渐地被运用到城市绿色基础设施的规划建设当中,发掘能提供高生态效益的绿色屋顶潜力区域是增加城市生态空间有效途径.以提供城市空气净化、雨水截留、生物栖息地、热环境调节四个方面的生态服务为目标,借助GIS平台确定醴陵市绿色屋顶生态空间潜力区域并提出规划策略,为醴陵市以绿色屋顶形式完善城市生态空间结构...     

Metabolic profiling in validation of plasma biomarkers for green tea polyphenols

Green tea polyphenols (GTP) effectively protect against chronic diseases in various animal models but human studies have been inconclusive. GTP components and metabolites in body fluids have been suggested as potential biomarkers, but validation of these biomarkers has rarely been done in human populations. A randomized, double-blinded, and placebo-controlled phase IIa chemoprevention study with GTP was conducted in 120 human subjects for 3 months. To validate GTP biomarker profiles, plasma samples were collected at baseline, 1-month, and 3-month and were analyzed by HPLC-Coularray electrochemical detection (ECD) for specific GTP components as well as for non-targeted metabolites. The levels of 2 GTP components, epigallocatechin-3-gallate (EGCG) and epicatechin-3-gallate (ECG), were homogenous at baseline (p > 0.45) but were significantly elevated (p < 0.01) by GTP treatment. Metabolic profiling identified 106 metabolites, and 56 of them were chosen to construct discriminant functions (DFs) based on the data at 3 months. The DFs clearly separated the placebo, 500 mg GTP, and 1000 mg GTP groups with an accuracy rate of 97.3%. When the DFs were applied to the combined baseline and 1-month data, the accuracy rate was 62.9% in classifying subjects into the 3 intervention groups. DFs derived from 1-month data showed similar results. Overall, this study validated plasma EGCG and ECG as reliable biomarkers for GTP consumption, and found metabolic profiles effective in discriminating different GTP dosages.     

A review of methods for characterizing the environmental consequences of actions in life cycle assessment

Understanding the environmental consequences of actions is becoming increasingly important in the field of industrial ecology in general, and in life cycle assessment (LCA) more specifically. However, a consensus on how to operationalize this idea has not been reached. A variety of methods have been proposed and applied to case studies that cover various aspects of consequential life cycle assessment (CLCA). Previous reviews of the topic have focused on the broad agenda of CLCA and how different modeling frameworks fit into its goals. However, explicit examination of the spectrum of methods and their application to the different facets of CLCA are lacking. Here, we provide a detailed review of methods that have been used to construct models of the environmental consequences of actions in CLCA. First, we cover the following structural modeling approaches: (a) economic equilibrium models, (b) system dynamics models, (c) technology choice models, and (d) agent‐based models. We provide a detailed review of particular applications of each model in the CLCA domain. The advantages and disadvantages of each are discussed, and their relationships with CLCA are clarified. From this, we are able to map these models onto the established aspects of CLCA. We learn that structural models alone are not sufficient to quantify the uncertainty distributions of underlying parameters in CLCA, which are essential components of a robust analysis of consequences. To address this, we provide a brief introduction to a counterfactual‐based causal inference approach to parameter identification and uncertainty analysis that is emerging in the CLCA literature. We recommend that one potential research path forward is the establishment of feedback loops between empirical estimates and structural models.     

Green land: Multiple perspectives on green algal evolution and the earliest land plants

Green plants, broadly defined as green algae and the land plants (together, Viridiplantae), constitute the primary eukaryotic lineage that successfully colonized Earth's emergent landscape. Members of various clades of green plants have independently made the transition from fully aquatic to subaerial habitats many times throughout Earth's history. The transition, from unicells or simple filaments to complex multicellular plant bodies with functionally differentiated tissues and organs, was accompanied by innovations built upon a genetic and phenotypic toolkit that have served aquatic green phototrophs successfully for at least a billion years. These innovations opened an enormous array of new, drier places to live on the planet and resulted in a huge diversity of land plants that have dominated terrestrial ecosystems over the past 500 million years. This review examines the greening of the land from several perspectives, from paleontology to phylogenomics, to water stress responses and the genetic toolkit shared by green algae and plants, to the genomic evolution of the sporophyte generation. We summarize advances on disparate fronts in elucidating this important event in the evolution of the biosphere and the lacunae in our understanding of it. We present the process not as a step-by-step advancement from primitive green cells to an inevitable success of embryophytes, but rather as a process of adaptations and exaptations that allowed multiple clades of green plants, with various combinations of morphological and physiological terrestrialized traits, to become diverse and successful inhabitants of the land habitats of Earth.     

Green batteries for clean skies: Sustainability assessment of lithium-sulfur all-solid-state batteries for electric aircraft

The use of novel battery technologies in short-haul electric aircraft can support the aviation sector in achieving its goals for a sustainable development. However, the production of the batteries is often associated with adverse environmental and socio-economic impacts, potentially leading to burden shifting. Therefore, this paper investigates alternative technologies for lithium–sulfur all-solid-state batteries (LiS-ASSBs) in terms of their contribution to the sustainable development goals (SDGs). We propose a new approach that builds on life cycle sustainability assessment and links the relevant impact categories to the related SDGs. The approach is applied to analyze four LiS-ASSB configurations with different solid electrolytes, designed for maximum specific energy using an electrochemical model. They are compared to a lithium–sulfur battery with a liquid electrolyte as a benchmark. The results of our cradle-to-gate analysis reveal that the new LiS-ASSB technologies generally have a positive contribution to SDG achievement. However, the battery configuration with the best technical characteristics is not the most promising in terms of SDG achievement. Especially variations from the technically optimal cathode thickness can improve the SDG contribution. A sensitivity analysis shows that the results are rather robust against the weighting factors within the SDG quantification method.     

A simple differential pulse polarographic method for the determination of thymoquinone in black seed oil

A reliable and simple differential pulse polarographic method is described for the determination of thymoquinone in black seed oil. The polarographic behaviour of thymoquinone was examined in various buffer systems over the pH range 5.0-10.0. Thymoquinone is reduced in a single, reversible peak at the dropping mercury electrode. The differential pulse polarogram showed a distinct peak in S?rensen buffer:methanol (3:7, v/v; pH 8.5) at a peak potential of -0.095 V (vs. silver/silver chloride electrode), and a plot of peak height against concentration was found to be linear over the range 0.2-15.0 microg/mL (R = 0.9998). The limit of detection was calculated to be 0.054 microg/mL. The polarographic method has been applied to determine thymoquinone in two black seed oil preparations available on the Austrian pharmaceutical market.     

Cortisol in Poecilia latipinna: its identification and the validation of methods for its determination in plasma

In vitro studies in which head kidney of Poecilia latipinna was incubated with labelled precursors have shown that cortisol is the only corticosteroid that could be detected as being produced by this tissue. Cortisol levels have been measured in the plasma of Poecilia latipinna by three methods. The routine use of two rapid and comparatively simple methods, the competitive protein binding assay and the radioimmunoassay, have been validated in terms of the more rigorous double isotope dilution derivative assay.     

生态系统服务供需评估方法研究进展

生态系统服务供需评估可为识别地区均衡状况、生态权衡、生态管理、生态补偿提供依据,是生态系统服务研究的热点之一。本文收集与梳理国内外生态系统服务评估方法,将其归纳为生态模型法、价值法、参与法和经验统计模型法四大类,从供需评估角度、适用尺度范围和数据要求等方面,比较了各类方法在生态服务评估中的应用情况及优缺点。四类方法应用以评估服务供给为主,而在需求评估中的应用较少。生态模型法与价值法适用尺度范围广,涵盖从局地尺度到全球尺度,参与法与经验统计模型法则多用于中小尺度。生态模型法所需数据种类多,对数据要求高,评估精度也相对较高,在方法适用尺度、数据获取、推广应用等方面更具优势。未来生态系统服务供需评估方法研究需重点考虑以下三个方面:构建多样、标准供需指标体系,加强生态系统服务评估精确性研究,加强模型集成与开发。     

Finding fault with health risk assessment: A typology for risk assessment criticism

Although systematic, quantitative assessment of environmental health risks is a staple of regulatory decision-making, complaints regarding its perceived failures and shortcomings are an intrinsic feature of the policy landscape. In this article, we (a) catalog the classic criticisms of conventional health risk assessment, (b) create a typology that orders the critiques according to their focus on either input errors or output biases, and (c) identify selected allegations that fall within each category. We also note that the risk assessment–risk management paradigm has evolved over the past several decades, partially in accordance with the general direction and spirit of these classic critiques. The debate continues today along familiar lines invoking the traditional critiques and rebuttals outlined here.     

Development and validation of a fish-based index for the assessment of 'river health' in France

SUMMARY 1. In Europe, water policy is currently undergoing considerable change as emphasised by the recent European Water Framework Directive (WFD), which requires the restoration and maintenance of 'healthy' aquatic ecosystems by the assessment of their hydromorphological, chemical and biological characteristics. If the requirements of the WFD are to be met, effective biological tools are needed to measure the 'health' of rivers at scales large enough to be useful for management. These tools need to be ecologically based, efficient, rapid and applicable in different ecological regions. Among potential indicators, fish assemblages are of particular interest because of their ability to integrate environmental variability at different spatial scales. To meet the goals of the WFD, the French Water Agencies and the Ministry of the Environment initiated a research programme to develop a fish-based index that would be applicable nationwide.
2. A variety of metrics based on occurrence and abundance data and reflecting different aspects of the fish assemblage structure and function were selected from available literature and for their potential to indicate degradation.
3. Logistic and multiple linear regression procedures were applied, using an initial data set of 650 reference sites fairly evenly distributed across French rivers and defined by some easily measured regional and local characteristics, to elaborate the simplest possible response model that adequately explained the observed patterns of each metric for a given site.
4. Models obtained for each metric were validated using two independent data sets of 88 reference sites and 88 disturbed sites. These procedures allowed us to select the most effective metrics in discriminating between reference and disturbed sites.     

Developments of biobased plasticizers for compostable polymers in the green packaging applications: A review

The demand for biobased materials for various end-uses in the bioplastic industry is substantially growing due to increasing awareness of health and environmental concerns, along with the toxicity of synthetic plasticizers such as phthalates. This fact has stimulated new regulations requiring the replacement of synthetic conventional plasticizers, particularly for packaging applications. Biobased plasticizers have recently been considered as essential additives, which may be used during the processing of compostable polymers to enormously boost biobased packaging applications. The development and utilization of biobased plasticizers derived from epoxidized soybean oil, castor oil, cardanol, citrate, and isosorbide have been broadly investigated. The synthesis of biobased plasticizers derived from renewable feedstocks and their impact on packaging material performance have been emphasized. Moreover, the effect of biobased plasticizer concentration, interaction, and compatibility on the polymer properties has been examined. Recent developments have resulted in the replacement of synthetic plasticizers by biobased counterparts. Particularly, this has been the case for some biodegradable thermoplastics-based packaging applications.     

Identification of Key Sustainability Performance Indicators and related assessment methods for the carbon fiber recycling sector

The need for the extensive use of sustainability assessment as a standalone tool to evaluate the environmental, economic and social aspects of an activity be it at project, product, company or region level has resulted in the development of various methods and standards. There are several indicator issues to address each aspect of sustainability and it is not easy for decision makers to understand the result due to the use of multiple indicators. In this regard, the paper aims at the identification and combination of indicators allowing to assess the sustainability which is applicable to a carbon fiber recycling sector. Indicators selection were carried out by performing an extensive literature review on existing publications dealing with the different pillars of sustainability and setting a number of selection criteria to prioritize indicators that are relevant to the sector. For the environmental aspect global warming, acidification and human toxicity seem to be the most relevant. The social-economic aspect can be addressed through considering the resource impact assessment by considering the supply risk due to the geological scarcity of a resource and the potential supply disruption due to geopolitical and other social factors. The finding shows that three indicators have been identified enabling the assessment of the environmental pillar. Then the necessity to use extra resources indicators was shown and justified by the need of providing a shorter timeframe perspective as well as to consider the amount of fiber to be recycled in the future and also to determine the potential benefit provided by the creation of this sector to the resource strategy point of view. This will be made possible by using such method as the criticality assessment that enable the consideration of geological and geopolitical supply risk as well as the characterization of the system dependence to a specific resource.Finally, these results lead to the expression of the need to the development of a novel indicator assessing the criticality of carbon fibers as well as the expression of the necessity for further research on the socio-economic perspectives.     

Green Alternatives in Treatment of Liver Diseases: the Challenges of Traditional Medicine and Green Nanomedicine

Over the last decade, liver diseases have become a global problem, with approximately two million deaths per year. The high increase in the mortality rate of these diseases is mostly related to the limitations in the understanding of the evolutionary clinical cases of liver diseases, the low delivery of drugs in the liver, the non-specific administration of drugs, and the side effects generated at the systemic level by conventional therapeutic agents. Today it is common knowledge that phytochemicals have a high curative potential, even in the prevention and/or reversibility of liver disorders; however, even using these green molecules, researchers continue to deal with the same challenges implemented with conventional therapeutic agents, which limits the pharmacological potential of these friendly molecules. On the other hand, the latest advances in nanotechnology have proven that the use of nanocarriers as a delivery system for green active ingredients, as well as conventional ones, increases the pharmacological potential of these active ingredients due to their physicochemical characteristics (size, Zeta potential, etc.,) moldable depending on the therapeutic objective; in addition to the above, it should be noted that in recent years, nanoparticles have been developed for the specific delivery of drugs towards a specific target (stellar cells, hepatocytes, Kupffer cells), depending on the clinical state of the disease in the patient. The present review addresses the challenges of traditional medicine and green nanomedicine as alternatives in the treatment of liver diseases.