橘皮素与乳清蛋白的合用对短跑运动员冬训期间血清睾酮、皮质醇水平的影响*

目的:探讨补充橘皮素对短跑运动员冬训期间血清睾酮、皮质醇水平的影响。方法:将24名短跑运动员配对、随机分为试验组和对照组。冬训期间,试验组补充橘皮素补剂(含橘皮素200 mg),对照组补充安慰剂,为期4周。分别在每周第一天(T1、T2、T3、T4)及干预结束次日(T5)采集血液样本,检测血清睾酮、皮质醇、超氧化物歧化酶(SOD)、促肾上腺皮质激素(ACTH)等指标,并在干预开始和结束时测试身体成分。结果:干预4周后,①对照组的血清皮质醇水平上升,血清睾酮和SOD活性水平显著下降(P＜0.05);②试验组的血清皮质醇(P＜0.05)和ACTH(P＜0.01)均显著下降,血清睾酮维持稳定,SOD活性水平小幅上升;③试验组和对照组的肌肉量均有增加,但前者的增加量显著大于后者。结论:补充4周橘皮素能减少冬训期间高强度运动引起的机体氧化应激反应,维持机体血清睾酮水平稳定,抑制皮质醇过度分泌,促进肌肉合成。     

加味八珍汤联合PFNA术治疗老年股骨转子间骨折患者的临床效果及对血液流变学指标的影响

目的:研究加味八珍汤联合股骨近端防旋髓内钉(Proximal Femoral Nail Antirotation,PFNA)对老年股骨转子间骨折患者的临床效果及对术后隐性失血和血液流变学的影响。方法:选择2014年3月至2017年3月我院收治的68例接受PFNA治疗的转子间骨折者,将其随机平均分为观察组(34例,术后口服加味八珍汤颗粒7天)、对照组(34例)。对比两组总失血量、隐性失血量、隐性失血比例、血液流变学指标(血浆粘度及全血黏度低、中、高切)、不良反应等。结果:⑴两组各剔除2例,94.12%(64/68)的受试者完成研究。⑵两组总失血量相当,差异无统计学意义(P0.05);与对照组相比,观察组隐性失血量及隐性失血发生率均显著降低(P0.05)。⑶治疗前,两组血浆粘度及低、中、高切全血黏度比较差异无统计学意义(P0.05);治疗后,两组血浆粘度及低、中、高切全血黏度均显著低于本组治疗前(P0.05),且观察组以上指标均明显低于对照组(P0.05)。⑷观察组发生肌间静脉血栓1例,无药物过敏及毒副反应;对照组发生1例深静脉血栓、4例肌间静脉血栓。观察组不良反应率为3.125%,显著低于对照组(15.625%,P0.05)。结论:加味八珍汤既可显著减少老年股骨转子间骨折患者PFNA术后隐性失血,又能改善血流动力学、减少并发症。     

酪氨酸对男子血清促性腺激素和睾酮浓度的影响

给成年男子(n=5)口服酪氨酸300mg/日,共6天,第四天肌注 LRH-A 150μg/人,用RIA 法测定其血清 LH、FSH 和睾酮浓度。与对照组(n=5)相比,酪氨酸对 LH 和 FSH 的基础水平和 LRH-A 诱发的 LH 峰和 FSH 峰均无明显影响。睾酮的基础水平稍有升高,而酪氨酸对 LRH-A 引起的睾酮水平升高有明显的抑制作用。结果提示,酪氨酸可不通过垂体而直接作用于睾丸以影响睾酮的分泌。     

女子足球运动员比赛时心率和赛后血乳酸浓度的研究

在比赛条件下观察了12名女子足球运动员的心率和血乳酸的变化。上、下半场比赛时,心率在170次/分以上者分别占总记录次数的20.8％和13.0％,心率小于150次/分者则分别为37.5％和48.2％。上、下半场赛后血乳酸分别为33.9±8.29mg％和21.64±6.96mg％(P相似文献   

沙棘汁对大鼠骨骼肌自由基代谢和血液血红蛋白、肌酸激酶、睾酮的影响

目的:探讨沙棘汁对大鼠骨骼肌自由基代谢和血液部分生化指标的影响。方法:30只SD大鼠随机分为安静组、训练组、沙棘训练组,6周训练和补充沙棘汁后,测定大鼠骨骼肌和血液的有关指标。结果:沙棘训练组与训练组相比,运动至力竭的时间明显延长;骨骼肌抗氧化酶活性明显升高,丙二醛(MDA)含量极为显著降低;血睾酮(T)和血红蛋白(Hb)均明显升高;肌酸激酶(CK)显著降低。结论:沙棘汁能增强大鼠骨骼肌抗氧化能力,提高血睾酮和血红蛋白水平,延缓疲劳出现,提高其有氧耐力运动的能力。     

理气调补汤对运动性疲劳大鼠血清皮质醇和白细胞介素2影响的研究

目的:通过观察理气调补汤对运动性疲劳大鼠血清皮质醇(C)和白细胞介素2(IL-2)水平的影响,以期揭示中药理气调补汤促进运动性疲劳恢复的作用机理.方法:健康雄性SD大鼠30只,随机分为对照组(Ⅰ组)8只、单纯运动组(Ⅱ组)11只、中药运动组(Ⅲ组)11只.实验时Ⅲ组每天定时定量中药灌胃1ml/100g体重,Ⅰ组、Ⅱ组灌胃相同剂量的生理盐水.实验采用游泳运动训练方式.6周末力竭训练后24h分离血清测定C和IL-2含量.结果:Ⅲ组大鼠游泳至力竭的时间较Ⅱ组显著延长;Ⅱ组和Ⅲ组大鼠血清IL-2浓度均显著低于Ⅰ组,但Ⅲ组大鼠血清IL-2浓度显著高于Ⅱ组;血清C浓度Ⅱ组显著高于Ⅰ组,Ⅲ组较Ⅱ组显著降低.结论:大强度运动训练可能导致HPA轴释放过量皮质醇,从而使免疫功能受到抑制.理气调补汤能够调节疲劳大鼠的HPA轴适应性,降低C的过量释放,维持免疫功能稳定,延缓疲劳的发生.     

基于网络药理学探究苗药“四大血”对类风湿性关节炎自噬的影响

本研究皆在探究苗药“四大血”(Sidaxue, SX)调控类风湿性关节炎(rheumatoid arthritis, RA)中细胞自噬的分子机制。首先,构建胶原诱导型关节炎(collagen-induced arthritis, CIA)大鼠模型,SX给药21 d后计算大鼠胸腺指数和脾脏指数,观察大鼠膝关节滑膜组织病理改变,并检测大鼠血清白细胞介素-6(interleukin-6, IL-6)和白细胞介素-37(interleukin-37, IL-37)水平。进一步利用网络药理学分析SX调控RA中细胞自噬的可能分子机制,并借助Autodock vina软件进行分子对接验证,同时通过在线数据库预测SX主要活性成分的药学性质,结合分子对接评分初筛效应成分。最后,利用RT-qPCR和Western blot分别检测SX对CIA大鼠滑膜组织中磷脂酰肌醇3-激酶(phosphoinositide-3 kinase,PI3K)、蛋白激酶B(protein kinase B,AKT)、哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)及微管相关蛋白1轻链...     

加味八珍汤联合mFOLFOX6方案对晚期结直肠癌患者肿瘤标志物、免疫功能和外周血PI3K-Akt信号通路的影响

摘要 目的：探讨加味八珍汤联合mFOLFOX6方案对晚期结直肠癌患者肿瘤标志物、免疫功能和外周血磷脂酰肌醇3酶（PI3K）-丝苏氨酸蛋白激酶（Akt）信号通路的影响。方法：根据随机数字表法将广州中医药大学附属北碚中医院2020年9月至2022年1月期间收治的晚期结直肠癌患者96例分为对照组（48例,mFOLFOX6方案治疗）和研究组（48例,加味八珍汤联合mFOLFOX6方案治疗）。对比两组疗效、中医证候积分、卡式评分（KPS）、肿瘤标志物、免疫功能和外周血PI3K-Akt信号通路相关指标。结果：与对照组相比,研究组的临床总有效率升高（P<0.05）。研究组治疗后的中医证候总积分低于对照组,KPS评分高于对照组（P<0.05）。研究组治疗后的CD4⁺、CD4⁺/CD8⁺、免疫球蛋白（Ig）M、IgA高于对照组,CD8⁺低于对照组（P<0.05）。研究组治疗后的血清癌胚抗原（CEA）、糖类抗原199（CA199）、糖类抗原72-4（CA72-4）低于对照组（P<0.05）。研究组治疗后的外周血PI3K、Akt mRNA表达水平低于对照组（P<0.05）。两组不良反应发生率对比无差异（P>0.05）。结论：加味八珍汤联合mFOLFOX6方案治疗晚期结直肠癌的疗效显著,可有效阻止晚期结直肠癌的疾病进展,增强免疫力,降低血清肿瘤标志物水平,其疗效机制可能与调节外周血PI3K-Akt信号通路有关。     

不同支持物对“艾西丝”南瓜试管苗生根影响的研究

本试验以脱脂棉、琼脂、蛭石、珍珠岩和河沙为支持物配制的“艾西丝”南瓜试管苗生根培养基,观察到脱脂棉培养基具有诱导生根率高,生根质量好,移栽成活率高的效果,且重复利用率高,是优质、廉价的培养基支持物。     

“716”中药复方对BALB/c小鼠NK细胞活性影响的研究

临床观察表明,“716”中药复方对儿童易患的多种病毒性疾病具有较好的疗效。本文研究了“716”中药复方对BALB/c小鼠NK细胞活性的影响,以探讨其作用机制。结果显示:1)体外,当药物浓度≥1.25mg/ml时,NK细胞对L929细胞的溶活解性有较大的提高;2)体内,实验组动物NK细胞对L929细胞溶解性活显著增加(P<0.01)。提示,“716”中药复方对NK细胞活性的促进作用可能是其重要的作用机制之一。     

Relationship between the 2D:4D and prenatal testosterone,adult level testosterone,and testosterone change: Meta-analysis of 54 studies

The ratio between the hands' second to the fourth finger (2D:4D) is commonly hypothesized to result from prenatal testosterone. The 2D:4D has also been hypothesized to relate to adult-level testosterone and, more recently, to the testosterone response to a challenging situation. In the present work, we tested these core assumptions. Drawing from, in total, 54 studies and 8077 participants, we investigated whether the 2D:4D is related to adult level testosterone (44 studies), testosterone change (6 studies), and prenatal testosterone (10 studies). We found no evidence of the relationship between the above testosterone types and digit ratios. Furthermore, there was no relationship between testosterone and the right and left 2D:4D, male and female 2D:4D, and the 2D:4D and testosterone measurement (i.e., measured in blood or saliva). However, we found some evidence that prenatal testosterone measured in amniotic fluid (but not cord blood) might be related to the digit ratios—further studies are necessary to validate this observation. In summary, considering the current state of knowledge, any conclusions drawn from the assumption of the digit ratios as the proxy for testosterone (prenatal, adult level, or testosterone change under a challenging situation) warrant great caution.     

The influence of some sample handling factors on progesterone and testosterone analysis in goats

This study validated the use of commercially available radioimmunoassay kits for measuring the circulating progesterone and testosterone levels of goats. Progesterone and testosterone levels were then assayed in plasma which was collected from 23 does and 8 bucks. Collections from each animal were divided into three sodium fluoride-potassium oxalate (F/OX), one heparin, and one EDTA tubes and also into a tube without anticoagulant. Plasma from an F/OX tube was separated immediately from the blood cells by centrifugation. Serum or plasma was also separated after storage for 24 hours with F/OX, heparin or EDTA anticoagulant at 22 degrees C or with F/OX at 5 degrees C. A significant decline in assayable progesterone occurred in samples stored at 22 degrees C with each anticoagulant used and in the serum sample. Samples stored at 5 degrees C for 24 hours with F/OX anticoagulant contained concentrations of progesterone which did not differ significantly from those in samples where plasma was removed immediately. Assayable testosterone did not change with the anticoagulant used or vary with the storage temperature when F/OX tubes were stored at 5 degrees C and 22 degrees C for 24 hours. Results indicate that sample storage does influence levels of measured progesterone but not testosterone in goats. Progesterone assay is best done on plasma which is immediately separated from blood cells or on samples which are stored at 5 degrees C.     

Bacteria in the oral mucosa and its effects on the measurement of cortisol, dehydroepiandrosterone, and testosterone in saliva

Bacteria load in saliva was experimentally manipulated, and the consequences for the measurement of salivary testosterone (T), dehydroepiandrosterone (DHEA), and cortisol (C) were examined. Healthy adults (n = 19) donated the first saliva sample upon rising after which they rinsed their mouths with water, waited 10 min, and donated a second sample. Samples were either left untreated or passed through a 0.22-microm filter and then frozen at -80 degrees C or incubated at room temperature (RT) for 10 days. Aliquots of each sample were cultured on agar to determine baseline and post-incubation (or freezing) bacteria load. Bacteria counts were not significantly influenced by rinsing (with water), were substantially reduced by filtration, and increased by incubation at RT. Average levels of salivary T and C, but not DHEA, were significantly lower in samples stored at RT than samples frozen the day of collection. The change in bacteria count induced by storing samples at RT was associated with a change in testosterone but not cortisol or DHEA. When samples were passed through a 0.22-microm filter bacteria counts were reduced, and the association between bacteria and testosterone was reduced to non-significant. These findings contribute to a growing body of literature revealing that the process of sample collection, storage, and handling can dramatically influence the accuracy of information generated when salivary biomarkers are integrated into research and clinical diagnostics.     

Energetic reserves, leptin and testosterone: a refinement of the immunocompetence handicap hypothesis

The immunocompetence handicap hypothesis (ICHH) assumes that testosterone (T), required for the expression of sexual traits, can also incur a cost due to its immunosuppressive properties. However, T-dependent immunosuppression could also arise as an indirect consequence of energy reallocation from the immune system to other metabolic demands. Leptin is mostly produced in lipogenic tissues and its circulating level is positively correlated with the amount of lipid reserves. Leptin also has an important role as immunoenhancer and we suggest that this hormone could play a role as a mediator of the immunosuppressive effect of testosterone. In particular, we propose that only the individuals able to maintain large lipid reserves (with high leptin levels), while sustaining high testosterone levels, might be able to develop sexual displays without an impairment of their immune defences. Here, we tested one of the assumptions underlying this extension of the ICHH: leptin administration should attenuate testosterone-induced immunosuppression. T-implanted and control male zebra finches (Taeniopygia guttata) received daily injections of leptin or phosphate buffered saline. T-implants initially depressed the phytohaemagglutinin-induced immune response. However, T-birds injected with leptin enhanced their immune response to the level of control birds. These results open a new perspective on the study of the ICHH.     

Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits

Pannon White (n=12) male rabbits (weight: 4050 to 4500 g, age: 9 months) received 2 ml of a suspension containing purified T-2 toxin by gavage for 3 days. The daily toxin intake was 4 mg/animal (0.78 to 0.99 mg/kg body weight (BW)). Control animals (n=12) received toxin-free suspension for 3 days. Since a feed-refusal effect was observed on the second day after T-2 administration, a group of bucks (n=10) were kept as controls (no toxin treatment) but on a restricted feeding schedule, that is, the same amount of feed was provided to them as was consumed by the exposed animals. On day 51 of the experiment (i.e. 48 days after the 3-day toxin treatment), semen was collected, and pH, concentration, motility and morphology of the spermatozoa, as well as concentration of citric acid, zinc and fructose in the seminal plasma, were measured. After gonadotropin-releasing hormone (GnRH) analogue treatment, the testosterone level was examined. One day of T-2 toxin treatment dramatically decreased voluntary feed intake (by 27% compared to control, P<0.05) and remained lower (P<0.05) during the first 2 weeks after the withdrawal of the toxin. BW of the contaminated rabbits decreased by 88% on days 17 and 29 compared to controls (P<0.05). No effect of toxin treatment was detected on pH and quantity of the semen or concentration of spermatozoa. The ratio of spermatozoa showing progressive forward motility decreased from 65% to 53% in the semen samples of toxin-treated animals compared to controls (P>0.05). The ratio of spermatozoa with abnormal morphology increased (P<0.05) in the ejaculates collected from the toxin-treated animals. T-2 toxin applied in high doses decreased the concentration of citric acid in seminal plasma (P<0.05). No effect of T-2 toxin on the concentrations of the other seminal plasma parameters (fructose and zinc) was observed. T-2 toxin decreased the basic testosterone level by 45% compared to control (P<0.01) and resulted in lower (P<0.05) GnRH-induced testosterone concentration. Feed restriction, that is, less nutrient intake, resulted in more morphologically abnormal spermatozoa in the semen, but it did not cause significant loss in BW, motility of the spermatozoa, composition of the seminal plasma or testosterone concentration--its effect needs further examination.     

Women's intercollegiate volleyball and tennis: Effects of warm-up, competition, and practice on saliva levels of cortisol and testosterone

In virtually all sports, participants “warm-up” prior to formal competition. Women athletes from a highly ranked varsity college volleyball team and, in a second study, a highly ranked varsity college tennis team gave saliva samples before warm-up, at mid-warm-up (volleyball) or after warm-up (tennis), and immediately after intercollegiate competition. For volleyball and tennis, warm-up was associated with a substantial elevation in saliva levels of testosterone which was carried over through the period of actual competition. Cortisol levels were relatively unchanged during warm-up, but typically rose during competition. Thus, as women prepare for athletic competition by warming up, testosterone levels rise in apparent anticipation of the coming contest and then remain high through the period of play. In volleyball and tennis, after-practice testosterone level was significantly higher than before-practice level, and practice session increases in testosterone (but not cortisol) were positively correlated with increases in testosterone during intercollegiate competition. When practice and competitive play share as yet undetermined key elements, individual differences in this endocrine response to “competition” appear stable across practice and intercollegiate competition.     

Seasonal changes in epididymis and the effects of FSH and testosterone in the spiny-tailed lizard,Uromastix hardwicki

Seasonal changes in epididymal weight and histology were studied in relation to testicular function in the adult spiny-tailed lizard, Uromastix hardwicki, over a period of 1 year. The eipdidymal weights, tubular diameter, and epithelial height increased in March, reaching a peak in April. This peak coincided with sperm maturation, elevated plasma testosterone levels, and release of sperm into the epididymis. The epididymal weights decreased in May following a sudden regression of the testis early in the month. The epididymal weights decreased further during June and remained low until February. The diameter of the duct and the height of the epithelial cells also decreased in May and the epididymal epithelium maintained a low histological profile from June to February. The fall testicular recrudescence was not accompanied by a change either in the weight or the histological structure of the epididymis. Administration of oFSH (0.1 mg) daily for 7 days during the sexually quiescent period induced a significant increase in the weight of the epididymis and epithelial height of the duct. Administration of testosterone alone, (2.0 mg) daily for the same period and under identical conditions, did not induce a change in the weight of epididymis or its histology. A possible permissive role of gonadotrophin in the hormonal regulation of the lizard epididymis has been suggested.     

Genetics,ontogeny, and testosterone inducibility of aldehyde oxidase isozymes in the mouse: Evidence for two genetic loci (Aox-1 and Aox-2) closely linked on chromosome 1

Null-activity and low-activity variants for the liver supernatant isozymes of aldehyde oxidase (designated AOX-1 and AOX-2) were observed in inbred strains and in Harwell linkage testing stocks of Mus musculus. The genetic loci determining the activity of these isozymes (designated Aox-1 and Aox-2, respectively) are closely linked on chromosome 1 near Id-1 (encoding the soluble isozyme of isocitrate dehydrogenase). Linkage data of Aox-1 with Id-1 and Dip-1 (encoding a kidney peptidase) demonstrated that this gene coincides with or is closely linked to Aox (Watson et al., 1972). Ontogenetic analyses demonstrated that liver AOX-1 appeared just before birth and increased in activity during postnatal development, whereas liver AOX-2 was observed only during postnatal development. Adult male livers exhibited higher AOX-1 and AOX-2 activities than adult female livers. Both isozymes were significantly reduced in activity by castration of adult males and increased following testosterone administration to castrated males and normal female mice.