Phylogeny of ground beetles subgenus Nialoe (s. lat.) Tanaka (Coleoptera: Carabidae; genus Pterostichus): A molecular phylogenetic approach

We constructed a phylogeny of the ground beetle subgenus Nialoe ( s. lat. ), genus Pterostichus (Coleoptera: Carabidae) based on two mitochondrial (cytochrome oxidase I and 16S ribosomal DNA) and one nuclear (28S ribosomal DNA) gene sequences. Thirty-three representative species of the group and three outgroup species were analyzed. The resultant trees (maximum parsimonious, maximum likelihood and Bayesian trees of the combined data of the three gene sequences) indicated that there are two large and three small lineages in the group, some of which were supported by a previous morphology-based phylogeny. In all the analyses, the small lineage composed of two Korean species is sister to the rest of the subgenus, but relationships of other four lineages differed among the analyses and remained unresolved. The implications of the present results are discussed in terms of taxonomy and biogeography of the group.     

Phylogenetic studies of the subgenus Nialoe (s. lat.) (Coleoptera, Carabidae, Genus Pterostichus), part 2: The Asymmetricus species group

Pterostichus (Nialoe) asymmetricus Bates and its allies are revised based on the membranous parts of their genitalia. Four new taxa, P. (N.) ovaliphallus sp. nov., P. (N.) fujimurai ibukiyamanus ssp. nov., P. (N.) shotaroi kiimontanus ssp. nov., and P. (N.) basilobatus sp. nov. are described. A cladistic analysis based on 31 morphological characters reveals that Daisenilaoe (s. str.) Nakane and Straneo is included as one clade within Nialoe (s. str.). The speciation and dispersal process of this species group are also discussed.     

A new classification for the subgenera of the genus Acanthophthirius Perkins,with descriptions of twelve new taxa (Acarina,Trombidiformes, Myobiidae)

The myobiid genus Acanthophthirius Perkins, to date comprising four subgenera, is reviewed and divided into just two subgenera, the nominate subgenus and Myotimyobia Fain. The male genital shield and female opisthogastric sclerites, which are here considered to be part of female genitalia, are adopted as the criteria for dividing the subgenera. These structures are essentially the same in form and position in the subgenus Acanthopthirius in its revised sense, while they are both more heterogeneous in species of the redefined subgenus Myotimyobia. The subgenera Acanthophthirius Fain and Chiromyobia Fain are thought to represent species-groups in the nominate subgenus, named respectively the etheldredae and miniopteri groups. The following one new subspecies and 11 new species are described: A. (A.) womersleyi eptesicus, A. (A.) glauconycteris, A. (A.) mauritaniensis, A. (A.) philetoris, A. (A.) otonycteris, A. (A.) steatocaudatus, A. (A.) nyctophilis, A. (M.) vagus, A. (M.) longus A. (M.) baueris, A. (A.) hesperopteris (female only) and A. (M.) pixonixeos (female only). A. (M.) hanensis is synonymised with A. (M.) namurensis, and A. (M.) capacini is emended to A. (M.) capaccinii and relegated to a subspecies of A. (M.) myotis. All the known and new species are assigned to their respective subgenera and shown in a table. Incongruent host-relationships of some of the mites are clarified, although not completely solved, by the introduction of the new classification for the subgenera.     

Two new species of Pterostichus Bonelli subgenus Pseudoferonina Ball (Coleoptera, Carabidae, Pterostichini) from the mountains of central Idaho, U.S.A

Two new species of Pterostichus Bonelli subgenus Pseudoferonina Ball, are described from the mountains of central Idaho: Pterostichus bousqueti Bergdahl [type locality = small tributaries of South Fork of Payette River watershed, ca. 1170 m (3840 ft), 44.0675°; -115.6822°, near Lowman, Salmon River Mountains, Boise County, Idaho, U.S.A.] and Pterostichus lolo Bergdahl [type locality = Cottonwood/Orogrande Creek, ca. 870 m (2850 ft), 46.5528°; -115.5522°, North Fork of Clearwater River watershed, Clearwater Mountains, near Bungalow, Clearwater County, Idaho, U.S.A.]. Males of Pterostichus bousqueti and Pterostichus lolo are easily distinguished from each other and the seven previously described Pseudoferonina species by the form of the median lobe of the aedeagus, and from most individuals of the other species of Pseudoferonina in Idaho by features of pronotal shape and macrosculpture. Both species appear to be obligate ripicolous hygrophiles, restricted in distribution primarily to the margins of small montane streams in forested areas. Widespread intensive stream surveys for Pseudoferonina over many years indicate the geographic ranges of both species are highly localized, and additional undescribed species may occur in Idaho.     

Notes on two species of the subgenus Lyrothorax Chaudoir (Coleoptera: Carabidae; genus Pterostichus), Pterostichus amagisanus Tanaka and Ishida and Pterostichus fujitai Tanaka and Ishida

Two species of the subgenus Lyrothorax Chaudoir (Coleoptera: Carabidae; genus Pterostichus ), Pterostichus amagisanus Tanaka and Ishida and Pterostichus fujitai Tanaka and Ishida, were revised based on the male endophallus (inner sac everted from aedeagus). P. amagisanus was newly recorded based on a single male from Kyushu, southwest Japan, far from its known distribution (Honshu; the Fuji-Hakone-Izu volcano area), although additional materials are necessary to confirm this record. Despite a highly disjunct distribution, no conspicuous difference was recognized in either external or genital characters between the materials from Honshu and Kyushu. The nominal species P. fujitai was separated into two species, P. fujitai (Honshu) and Pterostichus eoyoritomus sp. nov. (Shikoku; type locality: Mount Jingayama); these two species have some significant differences in the endophallic structures. Character states in male genitalia suggest a sister relationship between P. eoyoritomus sp. nov. and Pterostichus yoritomus Bates.     

An SEM study of the haptoral sclerites of the genus Gyrodactylus Nordmann, 1832 (Monogenea) following extraction by digestion and sonication techniques

Techniques for the preparation of haptoral sclerites of Gyrodactylus species for scanning electron microscopy are described. Enzymatic digestion using pepsin allows fresh, frozen and alcohol-fixed material to be examined, but some sclerites, such as the dorsal and ventral bars, are lost. Sonication of fresh and frozen material retained these accessory bars, including the ventral bar membrane, the guard or sickle filament loop on the marginal hooks and even on some occasions ligaments associated with the sclerites. The sclerites of Gyrodactylus spp. from Gasterosteus aculeatus (L.) (three-spined stickleback), Oncorhynchus mykiss (Walbaum) (rainbow trout) and Salvelinus alpinus (L.) (charr) are described from SEM observations following the use of these preparation techniques, and the articulation between the hamuli and bars is commented upon.     

中国冠果蝇属一新种和二新记录种及DNA条形码信息(双翅目:果蝇科)

基于形态学和分子遗传学证据,描述了冠果蝇属1新种Stegana(Stegana)helvippecta sp.nov.和2中国新记录种:S.(S.)furta(Linnaeus,1767)和 S.(S.)taba Okada,1971;利用41 条 DNA 条形码信息,分析了18种冠果蝇的遗传距离,并提供了中国产全部1...     

Cryptic species of the subgenus Morphnosoma Lutshnik (Coleoptera: Carabidae; genus Pterostichus) from Japan

Pterostichus thunbergi Morawitz and its related species of Japan are revised based on the structure of membranous parts of their genitalia for the first time, under the name of the thunbergi species group. This species group is monophyletic and is placed in the subgenus Morphnosoma Lutshnik. The monotypic subgenus Moritapterus Berlov, erected for P. thunbergi, is synonymized with Morphnosoma. Comparative study of the endophallus revealed that P. thunbergi michinoku Nakane is a junior synonym of P. habui and that four species should be recognized in this group. Three of them are sympatric with P. thunbergi and one of them, Pterostichus (Morphnosoma) robustistylis, is described as a new species. Molecular phylogeny based on the COI gene (mitochondrial [mt]DNA) supported its independence as a distinct species. The resultant trees of mtDNA and comparative study of morphology revealed that P. thunbergi is probably non‐monophyletic. In the present study we suggest the presence of further cryptic species of Morphnosoma in Japan.     

Molecular phylogenetics reveals Leontodon (Asteraceae, Lactuceae) to be diphyletic

The plastid matK gene, trnL/F spacer, and nuclear rDNA ITS were sequenced for 36 species of Leontodon and 29 taxa of related genera of tribe Lactuceae. Phylogenetic relationships inferred from the independent and combined data are largely congruent and reveal that Leontodon sensu lato (s.l.) as presently defined is diphyletic: L. subgenus Leontodon forms a clade with Helminthotheca, Picris and Hypochaeris as sister genera, whereas L. subgenus Oporinia appears as a separate clade with strong bootstrap support and is thus better treated as a separate genus. Previous sectional classifications of Leontodon s.l. are considered in the light of DNA and additional morphological and karyological data. Support is presented for a core group of Hypochaeridinae sensu stricto (s.s.) with the two clades of Leontodon s.l., Helminthotheca, Picris, and Hypochaeris, whereas Urospermum, Hyoseris, Aposeris, and Rhagadiolus appear to be positioned more distantly.     

A new species of Ascocotyle (Trematoda: Heterophyidae) from the South American sea lion, Otaria flavescens, off Patagonia, Argentina

We describe a new heterophyid species, Ascocotyle (Ascocotyle) patagoniensis n. sp., based on specimens collected from the intestines of the South American sea lion Otaria flavescens from Patagonia (Argentina). Ascocotyle (A.) patagoniensis n. sp. is distinguished from the other species of the subgenus by the number of circumoral spines, which are arranged in 2 rows of 18 to 23. The new species also differs from the other species in having a gonotyl without papillae. The specimens exhibited the widest seminal receptacle described for a species of this subgenus. Species of the subgenus Ascocotyle usually infect fish-eating birds or mammals in freshwater or brackish habitats. Ascocotyle (A.) patagoniensis n. sp. is the first species of the subgenus described from a marine mammal. However, no metacercariae of Ascocotyle spp. were found in 542 marine teleosts from 20 species collected in the same locality. The life cycle of the marine species from the Ascocotyle -complex infecting pinnipeds remains elusive.     

Description of Pintomyia limafalcaoae and Pintomyia antioquiensis,two new species of phlebotomine sand fly (Diptera,Psychodidae) from the Colombian Andes

Two new species of phlebotomine sand fly from Colombian Andes are described, belonging to the subgenus Pifanomyia of the genus Pintomyia. P. (P.) limafalcaoae sp. nov. for which both sexes are described, is assigned to the series pia while P. (P.) antioquiensis sp. nov., known only from the male, is included in the series verrucarum. The subgenus Pifanomyia is characterized and identification keys presented for the two new species.     

中国四川伪巨须隐翅虫亚属四新种(鞘翅目,隐翅虫科,巨须隐翅虫亚科)

记述中国四川巨须隐翅虫属Oxyporus Fablicius,伪巨须隐翅虫亚属subgenus Pseudoxyporus 4新种,其中,半黑伪巨须隐翅虫Oxyporus(Pseudoxyporus)heminigritus sp.nov.采自巴塘县竹笆笼自然保护区;九龙伪巨须隐翅虫O.(P.)jiulongus sp.nov.采自九龙县瓦灰山和木里县;端节伪巨须隐翅虫O.(P.)parajiulongus sp.nov.采自青川县唐家河自然保护区;肖氏伪巨须隐翅虫O.(P.)xiaoae sp.nov.采自冕宁县冶勒自然保护区。新种附有彩色整体外形图,其中的2个种配有特征图。编制了中国种的检索表。     

A plastid DNA phylogeny of the genus Acacia Miller (Acacieae, Leguminoseae)

Past classifications of the tribe Acacieae Rchb. are outlined and the confusion concerning the relationships of the three subgenera of Acacia Mill. are highlighted. A plastid DNA analysis of Acacieae shows that the genus Acacia is not monophyletic. Furthermore subgenera Acacia Vassal and Aculeiferum Vassal are sister taxa and neither appear closely related to subgenus Phyllodineae (DC.) Ser. Subgenera Acacia and Aculeiferum form a clade that is basal to a well-supported clade consisting of tribe Ingeae Benth. taxa, Faidherbia albida (Del.) A. Chev. and subgenus Phyllodineae. The series of relationships suggested by the cpDNA data contradicts previous investigations of the tribe. Possible explanations of this conflict are explored, and the taxonomic implications of the plastid DNA data set are considered.     

中国颊脊隐翅虫属二点与一点颊脊隐翅虫亚属三新种(鞘翅目,隐翅虫科,隐翅虫亚科)

记述中国颊脊隐翅虫属Quedius二点颊脊隐翅虫亚属Microsaurus和一点颊脊隐翅虫亚属Raphirus 3新种,李氏二点颊脊隐翅虫Quedius(Microsaurus)lii sp.nov.,肖氏二点颊脊隐翅虫Quedius(Microsaurus)xiaoae sp.nov.,和粪一点颊脊隐翅虫Quedius(Rphirus)coprobius sp.nov..李氏二点颊脊隐翅虫至今未归入任何种群,可能代表一个新群,肖氏二点颊脊隐翅虫隶属二点颊脊隐翅虫亚属的穆柯颊脊隐翅虫群mukuensis,粪一点颊脊隐翅虫为一点颊脊隐翅虫亚属的里颊脊隐翅虫群intricatus的成员.     

Revision of the subgenus Ceratitis (Pardalaspis) Bezzi, 1918 (Diptera, Tephritidae, Ceratitini)

Abstract. The fruit fly genus Ceratitis (Diptera: Tephritidae) comprises several important pest species attacking a wide range of unrelated fruits. In this paper the subgenus Ceratitis ( Pardalaspis ) Bezzi is revised. Ten species are recognized of which five are described as new: C.(P.)hamata sp.n., C.(P.)munroi sp.n., C.(P.)serrata sp.n., C.(P.)semipunctata sp.n. and C.(P.)zairensis sp.n. A key to both sexes is provided. All species are restricted to the Afrotropical Region and distributional and known host plant data are given.     

中国颊脊隐翅虫属附点颊脊隐翅虫亚属三新种(鞘翅目,隐翅虫科,隐翅虫亚科)

记述中国颊脊隐翅虫属Quedius附点颊脊隐翅虫亚属Distichalius 3新种,王氏附点颊脊隐翅虫Quedius(Distichalius)wangi sp.nov.,仙附点颊脊隐翅虫Quedius(D.)xiansp.nov.,和卧龙附点颊脊隐翅虫Quedius(D.)wolong sp.nov.。俯附点颊脊隐翅虫Q.(D.)lin Smetana和日本附点颊脊隐翅虫Q.(D.)pretiosus Sharp分别在中国大陆和重庆市是首次记录。3新种都隶属克什米尔附点颊脊隐翅虫群thekashmirensis group,该群为中国新纪录群。2新纪录种,俯附点颊脊隐翅虫和日本附点颊脊隐翅虫,分别是连附点颊脊隐翅虫群theannectens group和查特附点颊脊隐翅虫群thechaterjeei group的成员。     

Contributions to the karyological study of the genus Ranunculus L. subgenus Batrachium (DC.) A Gray from the Iberian Peninsula

DIOSDADO. J. C, PASTOR, J. E. & VALDÉS, B. 1993. Contributions to the karyological study of the genus Ranunculus L. subgenus Batrachium (DC.) A. Gray from the Iberian Peninsula. The somatic and gametic chromosome numbers and detailed chromosome morphology are presented for eight taxa of aquatic ranunculi from the Iberian Peninsula. Within the subgenus Batrachium diploid and tetraploid levels have been observed (2 n = 16, 32) the commoner of which is the diploid. From the karyological data relationships have been established between the subgenus Batrachium and subgenus Ranunculus.     

中国多刺蚁属驼背亚属系统分类研究（膜翅目：蚁科）

记述了在云南省南部西双版纳自然保护区热带雨林区域采集到的多刺蚁属Polyrhachis Smith驼背亚属Cyrtomyrma Forel 5新种：方肩多刺蚁P. (C.) cornihumera sp. Nov.,齿肩多刺蚁P. (C.) dentihumera sp. Nov.,圆肩多刺蚁P. (C.) orbihumera sp. Nov.,圆顶多刺蚁P. (C.) rotoccipita sp. Nov.,短胸多刺蚁P. (C.) brevicorpa sp. Nov.。目前为止在中国和中南半岛记载该亚属13种,其中中国已知9种。提供了中国和中南半岛已知13种的检索表。