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1.
1. The effects of heavy metals on lipid peroxidation in the gills and digestive gland of mussels exposed for six days to Cu2+, Cd2+ or Zn2+ (40 μg/l/animal) were investigated.2. In the tissues of Cu-exposed mussels a significant increase of the level of malondialdehyde (MDA), which is indicative of the peroxidative process, and a decrease of the concentration of glutathione were observed.3. Moreover, in the digestive gland of mussels, copper exposure results in an increase of other carbonyl compounds and in the lysosomal accumulation of lipofuscin granules.4. The exposure of mussels to Zn or to Cd did not elicit any of the above effects.5. The results are discussed in relation to the possible role that Cu-induced lysosomal lipofuscin accumulation may play in heavy metal detoxification. 相似文献
2.
Sexually mature male and female frogs, Rana temporaria L., taken from their natural habitat at the end of the hibernation period, were subjected to treatment with ultrasound of various duration. The ultrasound (frequency, 24 kHz, intensity 0.35 W/cm2) was applied over the whole body in a suitable water bath. Neurons of the nucleus infundibularis dorsalis, medulla oblongata, and the subfornical organ were studied. A considerable increase in the amount of autofluorescent lipofuscin pigment was observed in nerve cells of various regions of the brain of the treated animals. The largest amounts of the lipofuscin pigment were found in those treated for one hour and killed on the fourteenth day following sonication. 相似文献
3.
We have observed pigmented cytoplasmic granules, with the characteristic staining properties of lipofuscin (ceroid, "wear-and-tear") pigment, in newborn human liver. The pigment is found at the periphery of the lobule in hepatocytes and some bile ductular cells. It is acid-fast, PAS-positive after diastase digestion, slightly argyophilic and sudanophilic, and markedly Schmorl's- and peroxidase positive in paraffin sections. Difficult to see in sections stained with hematoxylin and eosin, the pigment can be detected in unstained sections. The granules also resemble lipofuscin found in adult tissues, in their ultra-structural and enzymatic properties. They are polymorphic, contain granular material of moderate and high electron opacity, and are delimited by a single membrane. Acid phosphatase and β-glucuronidase activities are visualized in the newborn granules, identifying them as lysosomes. The granules also contain copper and, to a much lesser extent, iron. The accumulation of lipofuscin pigment in lysosomes in many tissues correlates well with aging, and this process has been interpreted as a reflection of cellular degeneration or wear-and-tear. However, the presence of lipofuscin granules as a constant component of neonatal liver suggests that they are not a measure of cellular senescence. 相似文献
4.
A quantitative study of the lipofuscin content was carried out by image analysis in brains of known-age, pond-reared Penaeus japonicus (Crustacea, Decapoda) with the aim of assessing the applicability of the lipofuscin technique as an estimator of the physiological age in penaeids. With this purpose, three distinct measurements of lipofuscin levels (% area fraction, granule density and mean granule size) were recorded in ten sections of the olfactory lobe cell mass (OLCM) per animal. The image analysis was based on the autofluorescence emitted by the pigment, which accentuates the contrast between the lipofuscin granules and the background tissue. The concentration of lipofuscin increased significantly with age and was independent of sex. The relationship between age and lipofuscin concentration (area fraction and granule density) was best described by a seasonalized von Bertalanffy function, since the accumulation rate of the pigment dramatically slowed down in fall-winter, probably as a result of reduced seasonal metabolism. The present results confirm the potential of the lipofuscin method in the estimation of physiological age in penaeids and suggest that the application of this methodology can be useful in studies of age structure in wild populations and in the assessment of natural resources. J. Exp. Zool. 286:120-130, 2000. 相似文献
5.
A2E, an important constituent of lipofuscin in human retinal pigment epithelium (RPE), is thought to mediate light-induced oxidative damage associated with aging and other ocular disorders. Ocular carotenoids in overlying retinal tissues were measured by HPLC and mass spectrometry and were correlated with levels of RPE A2E. We observed a statistically significant increase in total A2E levels in human RPE/choroid with age, and A2E levels in macular regions were approximately 1/3 lower than in peripheral retinal regions of the same size. There was a statistically significant inverse correlation between peripheral retina carotenoids and peripheral RPE/choroid A2E. Prospective carotenoid supplementation studies in Japanese quail demonstrated nearly complete inhibition of A2E formation and oxidation. These findings support current recommendations to increase dietary intake of xanthophyll carotenoids in individuals at risk for macular degeneration and highlight a new potential mechanism for their protective effects—inhibition of A2E formation and oxidation in the eye. 相似文献
6.
Occurrence of the autofluorescent pigment, lipofuscin, in polar crustaceans and its potential as an age marker 总被引:2,自引:0,他引:2
In crustaceans, the lack of reliable methods often prevents the determination of individual age. The quantification of the autofluorescent age pigment, lipofuscin, has revealed promising results in boreal and tropical species. We studied the presence of morphological lipofuscin and its possible application as an age marker in five Arctic and five Antarctic species, comprising decapods, amphipods and a euphausiid. Lipofuscin granules were located in the brain, using confocal fluorescence microscopy, and quantified from digital images. The pigment was found in 94 of 100 individuals and in all 10 species, and granules occurred in easily detectable amounts in 5 species. Two scavenging amphipod species, the Antarctic Waldeckia obesa and the Arctic Eurythenes gryllus, revealed the most conspicuous and numerous granules. There was a broad, though weak, correlation of lipofuscin concentration with individual body size within a species, but not with absolute body size of one species compared to another. In larvae of the decapod Chorismus antarcticus, lipofuscin accumulation was quantified over the 1st 4 months after larval release. Morphological lipofuscin is a potential index of age in those investigated species with a sufficient accumulation rate of the pigment. 相似文献
7.
Action spectra for the photoconsumption of oxygen by human ocular lipofuscin and lipofuscin extracts
Pawlak A Rózanowska M Zareba M Lamb LE Simon JD Sarna T 《Archives of biochemistry and biophysics》2002,403(1):59-62
The action spectra for the photoconsumption of oxygen by lipofuscin isolated from human retinal pigment epithelium cells and liposomal suspensions containing extracts of lipofuscin are reported. The lipofuscin and lipofuscin extract action spectra are similar, demonstrating the phototoxic constituents of lipofuscin are present in the lipofuscin solvent extract. 2-[2,6-Dimethyl-8-(2,6,6-trimethyl-1-cyclohexen-1-yl)-1E,3E,5E,7E-octatetraenyl]-1-(2-hydroxyethyl)-4-[4-methyl-6-(2,6,6-trimethyl-1-cyclohexen-1-yl)-1E,3E,5E-hexatrienyl]-pyridinium (A2E), present in both intact granules and the solvent extract, has been invoked as an important contributor to the phototoxicity of lipofuscin. The action spectrum for oxygen photoconsumption by A2E follows its absorption spectrum but does not resemble the action spectrum for photoconsumption of oxygen by lipofuscin granules or lipofuscin extract. These results combined with recently reported experimental studies on the aerobic photoreactivity of A2E indicate that it is not a major contributor to the phototoxicity of lipofuscin. 相似文献
8.
In vivo effects of diethylhydroxylamine (DEHA) on lipid peroxidation and lipofuscin formation in the nervous tissues of rat have been investigated. Rats were fed DEHA for 30, 60 and 90 days and lipid peroxidation levels and lipofuscin concentration measured in cerebellum, brain stem and spinal cord. Lipofuscin contents were also assessed histochemically. The results showed that the drug caused a significant reduction in lipid peroxidation level and lipofuscin concentration related to ageing. 相似文献
9.
Hiroyuki Shimasaki Takeshi Nozawa O.S. Privett W.R. Anderson 《Archives of biochemistry and biophysics》1977,183(2):443-451
Studies are reported on the detection of fluorescent substances and their relationship to the accumulation of the aging pigment and lactate dehydrogenase (LDH) isoenzyme composition in tissues of the rat. Comparison of fluorescent substances in nine tissues in 6- and 100-week-old rats by thin-layer chromatography showed that one substance in particular accumulated with age. This substance exhibited the typical excitation-fluorescence spectrum of lipofuscin pigments and its detection in tissues correlated closely with histologic analysis of the aging pigment. Accordingly, it was designated as an age-related fluorescent substance (ARFS). Because of the high intensity of its fluorescence and its relatively small mass, it appeared to be a constituent of aging pigment that was dissociated and extracted upon treatment of the tissue with chloroform-methanol. Histochemical analysis revealed the presence of both hemosiderin deposits and a golden brown pigment which was devoid of iron. These substances as well as the ARFS appeared to accumulate more in tissues which were classified generally as aerobic based on their LDH isoenzyme composition. 相似文献
10.
Neurons of Aplysia californica are naturally pigmented and the pigment accumulates with age. In the present study the pigment was examined in the same neuron from Aplysia of three postmetamorphic ages: young, sexually mature, and old. The large central neuron, R2, was examined by light and electron microscopy to determine if the pigment possessed properties similar to lipofuscin pigment seen in aging mammalian neurons. We used the same microscopic techniques that demonstrate the presence of lipofuscin in mammalian neurons. Light microscopic studies demonstrated a regional correlation between autofluorescence, staining with Sudan Black, and the naturally occurring pigment in old R2s. Electron microscopic studies revealed the presence of large vacuolated and lamellated membrane-bound bodies in the peripheral cytoplasm of old R2s, similar to those found in mammalian neurons. The bodies were located in the same region in which autofluorescence and Sudan Black staining were observed. Although the naturally occurring pigment accumulates with age, it acquires characteristics of lipofuscin pigment in the neurons of older sexually mature animals. The presence of these pigment characteristics can be used as an index of aging in Aplysia neurons as they are in mammalian neurons. 相似文献
11.
Zsolt Ablonczy Daniel Higbee Angus C. Grey Yiannis Koutalos Kevin L. Schey Rosalie K. Crouch 《Archives of biochemistry and biophysics》2013
The accumulation of lipofuscin in the retinal pigment epithelium (RPE) has been implicated in the development of age-related macular degeneration (AMD) in humans. The exact composition of lipofuscin is not known but its best characterized component is N-retinylidene-N-retinylethanolamine (A2E), a byproduct of the retinoid visual cycle. Utilizing our recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI–IMS)-based technique to determine the spatial distribution of A2E, this study compares the relationships of lipofuscin fluorescence and A2E in the murine and human RPE on representative normal tissue. To identify molecules with similar spatial patterns, the images of A2E and lipofuscin were correlated with all the individual images in the MALDI–IMS dataset. In the murine RPE, there was a remarkable correlation between A2E and lipofuscin. In the human RPE, however, minimal correlation was detected. These results were reflected in the marked distinctions between the molecules that spatially correlated with the images of lipofuscin and A2E in the human RPE. While the distribution of murine lipofuscin showed highest similarities with some of the known A2E-adducts, the composition of human lipofuscin was significantly different. These results indicate that A2E metabolism may be altered in the human compared to the murine RPE. 相似文献
12.
Majhi S Jena BS Patnaik BK 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2000,126(3):293-298
Oxidative damage was assessed through the estimation of lipid peroxides (LP) in the lungs of an ageing short-lived species of reptile, Calotes versicolor, commonly known as the garden lizard. Attempts were also made to trace its relationship with the age pigment, lipofuscin and the antioxidant ascorbic acid. While LP increased with advancing age the contents of both lipofuscin and ascorbic acid did not show appreciable change during maturation ( < 1-1 year old) but declined during senescence phase (1 to 2-4 year old). While the pattern of age associated changes in LP and ascorbic acid indicate similarity with the pattern observed in most of the mammals, the reduction of lipofuscin in older lizards is a significant departure from the common trend. 相似文献
13.
K M Liu C C Chang 《Proceedings of the National Science Council, Republic of China. Part B, Life sciences》1986,10(2):79-85
The ultrastructure of lipofuscin granules in neuroglial cells of the optic nerve of the Formosan Rock-Monkey was investigated by electron microscopy. In the cytoplasm of astroglial cells, numerous irregular lipofuscin granules were characterized by the presence of large lipid droplets, small electron-dense pigment granules, and some lamellar structures. The lipofuscin granules of the oligodendroglial cells were composed largely of dense, coarse pigment granules, multilinear structures, and a few small lipid droplets. The lipofuscin granules in microglial cells were characterized by numerous lipid droplets in various sizes, small electron-dense pigment granules, and prominent lamellar structures. It was reported that the lipofuscin granules are wear-and-tear materials and products from the cells in lower functional activity. However, our observations suggest that the presence of lipofuscin granules in the neuroglial cells of the optic nerve is likely a characteristic product of active phagocytosis. 相似文献
14.
O A Kutepova T A Voronina N N Zolotov 《Biulleten' eksperimental'no? biologii i meditsiny》1989,107(3):314-317
Molecular processes of biological aging could interact with molecular effects of ethanol, potentiating each other and culminating in accelerated aging process. According to this premise effect of antioxidant of 3-hydroxypyridine class (3-HP) on behaviour of 3-month-old mice after chronic alcoholisation was investigated. 5-month alcoholisation induced impairment of the process of learning in mice and accelerated accumulation of lipofuscin (age pigment) in the brain. 3-HP consumption (20-25 mg/kg/day in drinking water) during alcoholisation contributed to preservation of learning ability in mice and decreased lipofuscin accumulation in the brain in comparison with alcohol-treated mice. Effect of 3-HP may be due to its antioxidant properties and its ability to increase animals resistance to the extreme factors. 相似文献
15.
Summary Lipofuscin pigment formation and distribution in the Mes. N.5 neurons, trigeminal and spinal ganglia of male Wistar rats of 2, 14, 32 and 49 months as an indication of aging has been investigated. These intraneuronal pigment granules are found as early as 2 months in all the cells, and continue to accumulate in all the cells in varying amounts until the first year of life. The different rate at which lipofuscin accumulates probably shows the difference in the maturation of the functionally related cells. At later stages the obvious findings are complex pigment body formation and localization of the pigment bodies either at one pole as seen in the Mes. N. 5 neurons or arranged submembranously parallel to the long axis of the cells in the ganglia. The vacuolated lipofuscin pigment bodies are bound by a double limiting membrane and among the vacuoles are found tubular membranous structures resembling residual mitochondrial substructures. These findings suggest a mitochondrial origin of lipofuscin, rather than a lysosomal. The intracellular pigment bodies seen in the perineuronal satellite cells of peripheral ganglia appear to be signs of removal of lipofuscin from the ganglion cells.
Acknowledgements. We wish to thank Mr. J. Kirchhoff, Miss E. Heyder, Mr. W. Dresp and Mrs. M. del C. Weinrichter for the technical assistance, Mr. R. Dungan and Mrs. S. Ruelke for the photographic work. We are grateful to the DAAD and the Universitätsbund of the University of Göttingen for the financial assistance. This work was supported by the Deutsche Forschungsgemeinschaft, Grant No. G1 28, 16/17.DAAD fellow on leave from the Department of Anatomy, A.I.I.M.S., New Delhi 16, India. 相似文献
16.
Thampi P Rao HV Mitter SK Cai J Mao H Li H Seo S Qi X Lewin AS Romano C Boulton ME 《PloS one》2012,7(4):e34468
Age-related macular degeneration (AMD), a major cause of blindness in the elderly, is associated with oxidative stress, lipofuscin accumulation and retinal degeneration. The aim of this study was to determine if a 5-HT(1A) receptor agonist can reduce lipofuscin accumulation, reduce oxidative damage and prevent retinal cell loss both in vitro and in vivo. Autophagy-derived and photoreceptor outer segment (POS)-derived lipofuscin formation was assessed using FACS analysis and confocal microscopy in cultured retinal pigment epithelial (RPE) cells in the presence or absence of the 5-HT(1A) receptor agonist, 8-OH DPAT. 8-OH DPAT treatment resulted in a dose-dependent reduction in both autophagy- and POS-derived lipofuscin compared to control. Reduction in autophagy-induced lipofuscin was sustained for 4 weeks following removal of the drug. The ability of 8-OH DPAT to reduce oxidative damage following exposure to 200 μM H(2)O(2) was assessed. 8-OH DPAT reduced superoxide generation and increased mitochondrial superoxide dismutase (MnSOD) levels and the ratio of reduced glutathione to the oxidized form of glutathione in H(2)O(2)-treated cells compared to controls and protected against H(2)O(2)-initiated lipid peroxidation, nitrotyrosine levels and mitochondrial damage. SOD2 knockdown mice, which have an AMD-like phenotype, received daily subcutaneous injections of either saline, 0.5 or 5.0 mg/kg 8-OH DPAT and were evaluated at monthly intervals. Systemic administration of 8-OH DPAT improved the electroretinogram response in SOD2 knockdown eyes of mice compared to knockdown eyes receiving vehicle control. There was a significant increase in the ONL thickness in mice treated with 8-OH DPAT at 4 months past the time of MnSOD knockdown compared to untreated controls together with a 60% reduction in RPE lipofuscin. The data indicate that 5-HT(1A) agonists can reduce lipofuscin accumulation and protect the retina from oxidative damage and mitochondrial dysfunction. 5-HT(1A) receptor agonists may have potential as therapeutic agents in the treatment of retinal degenerative disease. 相似文献
17.
Boyer NP Higbee D Currin MB Blakeley LR Chen C Ablonczy Z Crouch RK Koutalos Y 《The Journal of biological chemistry》2012,287(26):22276-22286
The age-dependent accumulation of lipofuscin in the retinal pigment epithelium (RPE) has been associated with the development of retinal diseases, particularly age-related macular degeneration and Stargardt disease. A major component of lipofuscin is the bis-retinoid N-retinylidene-N-retinylethanolamine (A2E). The current model for the formation of A2E requires photoactivation of rhodopsin and subsequent release of all-trans-retinal. To understand the role of light exposure in the accumulation of lipofuscin and A2E, we analyzed RPEs and isolated rod photoreceptors from mice of different ages and strains, reared either in darkness or cyclic light. Lipofuscin levels were determined by fluorescence imaging, whereas A2E levels were quantified by HPLC and UV-visible absorption spectroscopy. The identity of A2E was confirmed by tandem mass spectrometry. Lipofuscin and A2E levels in the RPE increased with age and more so in the Stargardt model Abca4(-/-) than in the wild type strains 129/sv and C57Bl/6. For each strain, the levels of lipofuscin precursor fluorophores in dark-adapted rods and the levels and rates of increase of RPE lipofuscin and A2E were not different between dark-reared and cyclic light-reared animals. Both 11-cis- and all-trans-retinal generated lipofuscin-like fluorophores when added to metabolically compromised rod outer segments; however, it was only 11-cis-retinal that generated such fluorophores when added to metabolically intact rods. The results suggest that lipofuscin originates from the free 11-cis-retinal that is continuously supplied to the rod for rhodopsin regeneration and outer segment renewal. The physiological role of Abca4 may include the translocation of 11-cis-retinal complexes across the disk membrane. 相似文献
18.
Radu RA Hu J Yuan Q Welch DL Makshanoff J Lloyd M McMullen S Travis GH Bok D 《The Journal of biological chemistry》2011,286(21):18593-18601
Accumulation of vitamin A-derived lipofuscin fluorophores in the retinal pigment epithelium (RPE) is a pathologic feature of recessive Stargardt macular dystrophy, a blinding disease caused by dysfunction or loss of the ABCA4 transporter in rods and cones. Age-related macular degeneration, a prevalent blinding disease of the elderly, is strongly associated with mutations in the genes for complement regulatory proteins (CRP), causing chronic inflammation of the RPE. Here we explore the possible relationship between lipofuscin accumulation and complement activation in vivo. Using the abca4(-/-) mouse model for recessive Stargardt, we investigated the role of lipofuscin fluorophores (A2E-lipofuscin) on oxidative stress and complement activation. We observed higher expression of oxidative-stress genes and elevated products of lipid peroxidation in eyes from abca4(-/-) versus wild-type mice. We also observed higher levels of complement-activation products in abca4(-/-) RPE cells. Unexpectedly, expression of multiple CRPs, which protect cells from attack by the complement system, were lower in abca4(-/-) versus wild-type RPE. To test whether acute exposure of healthy RPE cells to A2E-lipofuscin affects oxidative stress and expression of CRPs, we fed cultured fetal-derived human RPE cells with rod outer segments from wild-type or abca4(-/-) retinas. In contrast to RPE cells in abca4(-/-) mice, human RPE cells exposed to abca4(-/-) rod outer segments adaptively increased expression of both oxidative-stress and CRP genes. These results suggest that A2E accumulation causes oxidative stress, complement activation, and down-regulation of protective CRP in the Stargardt mouse model. Thus, Stargardt disease and age-related macular degeneration may both be caused by chronic inflammation of the RPE. 相似文献
19.
The influence of lipofuscin granules and melanosomes from human retinal pigment epithelium on the light-induced photooxidation of cardiolipin liposomes and the generation of superoxide radicals was studied. Lipofuscin granules were able to stimulate, while melanosomes inhibited, the cardiolipin photooxidation. The visible light irradiation of both melanosomes and lipofuscin granules generated superoxide radicals with mean rates of 1.5 nmole/min/10(7) and 38 nmole/min/10(7) granules, accordingly. However, melanosomes but not lipofuscin granules reacted readily with superoxide radicals. Moreover, the rate constant of degradation of superoxide radicals in the presence of melanosomes was about five orders of magnitude higher than the rate constant of its photogeneration. Therefore, we propose that melanosomes in retinal pigment epithelium cells have a photoprotective role whereas lipofuscin granules may stimulate photodestructive reactions. 相似文献
20.
G Freund 《Life sciences》1979,24(2):145-151
Chronic ethanol consumption further accelerates age-related impairment of shuttle box avoidance learning in mice. The hypothesis was tested that the behavioral impairment is a result of brain lipofuscin pigment deposition, which may be accelerated by ethanol consumption and prevented by the antioxidant effects of pharmacological doses of vitamin E. Feeding an ethanol-containing liquid diet for 5 months did not increase the lipofuscin content when compared with mice pair-fed a liquid diet containing isocaloric amounts of sucrose or standard solid laboratory food containing nutritionally adequate amounts of vitamin E. Supplementation of diets with vitamin E decreased brain lipofuscin content in all groups but failed to prevent the age- or ethanol-induced learning deficit. There was no effect of chronic ethanol consumption on brain weights, DNA, RNA, or protein content.It is concluded that the age-related impairment of avoidance learning is accelerated by chronic alcohol consumption. At the molecular level this acceleration is not caused by an increased brain lipofuscin deposition nor is it prevented by the antioxidant effects of vitamin E. 相似文献