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1.
Montane temperate forests of the Cordillera de Piuchué Ecosystem Study, Isla Chiloé, Chile, are unaffected by air pollution, timber exploitation and agricultural clearing, and the current floristic assemblage has been relatively stable for the past 7500 years. The apparent absence of major perturbation at this location makes it an appropriate baseline site for ecosystem analysis. We measured soil bulk density, pH, soil organic C (SOC), total N, and NH4Cl–exchangeable cations (Ca+2, Mg+2, K+, Na+, Al+3) in 0–10 and 10–40 cm depth samples from 72 soil profiles representing three vegetation zones: Fitzroya cupressoides Forest, Pilgerodendron uvifera–Tepualia stipularis Forest, and Magellanic Moorland. Fitzroya and Pilgerodendron–Tepualia Forests were indistinguishable for all measured soil characteristics (P > 0.05, Dunn's multiple comparison test on ranked data); these included very high median SOC concentrations (0–10 cm = 49.6%) and correspondingly low bulk density values (0–10 cm = 0.07). Moorland soil median values (0-10 cm) were significantly higher for bulk density (0.12) and lower for SOC (28.5%), but not for total N (Forests = 0.99%, Moorland = 0.95%), resulting in lower median C:N ratios for the moorland (Forests = 44.7; Moorland = 30.3). Across both depths and all three vegetation zones regression analysis indicated that SOC was an excellent predictor (R2; = 0.93, P < 0.001) of (exchangeable Ca+2 + Mg+2 + K+ + Na+). Comparison with other old growth montane environments indicates that the Fitzroya and Pilgerodendron–Tepualia soil profiles are characterized by C:N ratios typical of other relatively unpolluted conifer forest soils (33.0–49.3). Soil profiles of representative polluted montane conifer forests have lower C:N ratios (16.2–23.5). Organic horizons from representative polluted montane conifer forests also retain fewer exchangeable base cations per unit SOC than are retained by organic horizons from the Cordillera de Piuchué forests.  相似文献   

2.
We compared hydrology, soils, and water quality of an agricultural field (AG), a two-year-old restored wetland (RW), and two reference ecosystems (a non-riverine swamp forest (NRSF) and a high pocosin forest (POC)) located at the Barra Farms Regional Wetland Mitigation Bank, a Carolina bay complex in Cumberland County, North Carolina. Our main objectives were to: 1) determine if the RW exhibited hydrology comparable to a reference ecosystem, 2) characterize the soils of the AG, RW, and reference ecosystems, and 3) assess differences in water quality in the surface outflow from the AG, RW, and reference ecosystems. Water table data indicated that the hydrology of the RW has been successfully reestablished as the hydroperiod of the RW closely matched that of the NRSF in 1998 and 1999. Jurisdictional hydrologic success criterion was also met by the RW in both years. To characterize soil properties, soil cores from each ecosystem were analyzed for bulk density (Db), total carbon (Ct), nitrogen (Nt), and phosphorus (Pt), extractable phosphate (PO4w), nitrogen (Nex), and cations (Caex, Mgex, Kex, Naex), as well as pH. Bulk density, Pt, Caex, Mgex, and pH were greatly elevated in the AG and RW compared to the reference ecosystems. Water quality monitoring consisted of measuring soluble reactive phosphorus (SRP), total phosphorus (TP), nitrate + nitrite (NOX), and total nitrogen (TN) concentrations in surface water from the AG, RW, and reference outflows. Outflow concentrations of SRP, TP, and NOX were highest and most variable in the AG, while TN was highest in the reference. This study suggested that while restoration of wetland hydrology has been successful in the short term, alteration of wetland soil properties by agriculture was so intense, that changes due to restoration were not apparent for most soil parameters. Restoration also appeared to provide water quality benefits, as outflow concentrations of SRP, TP, NOX, and TN were lower in the RW than the AG.  相似文献   

3.
Atmospheric N deposition is predicted to increase four times over its current status in tropical forests by 2030. Our ability to understand the effects of N enrichment on C and N cycles is being challenged by the large heterogeneity of the tropical forest biome. The specific response will depend on the forest’s nutrient status; however, few studies of N addition appear to incorporate the nutrient status in tropical forests, possibly due to difficulties in explaining how this status is maintained. We used a meta-analysis to explore the consequences of the N enrichment on C and N cycles in tropical montane and lowland forests. We tracked changes in aboveground and belowground plant C and N and in mineral soil in response to N addition. We found an increasing trend of plant biomass in montane forests, but not in lowland forests, as well as a greater increase in NO emission in montane forest compared with lowland forest. The N2O and NO emission increase in both forest; however, the N2O increase in lowland forest was significantly even at first time N addition. The NO emission increase showed be greater at first term compared with long term N addition. Moreover, the increase in total soil N, ammonium, microbial N, and dissolved N concentration under N enrichment indicates a rich N status of lowland forests. The available evidence of N addition experiments shows that the lowland forest is richer in N than montane forests. Finally, the greater increase in N leaching and N gas emission highlights the importance of study the N deposition effect on the global climate change.  相似文献   

4.
Background: Leaf traits are important in determining the capacity for a plant to acquire carbon, but few data are available for montane cloud forests in the Andes.

Aims: To investigate the changes in leaf traits along a large altitudinal transect (220–3600 m) from lowland to montane cloud forest in Peru.

Methods: We determined leaf mass per area (LMA, g m?2), leaf tissue density (L d, g cm?3), and foliar nitrogen (N) and phosphorus (P) content, both on a mass (N m and P m, %) and area (N a and P a, g m?2) basis for the most abundant species locally.

Results: LMA increased with altitude (62.8–169.4 g m?2), though overall, LMA was lower than in comparable tropical elevation gradients. N m declined significantly with altitude (2.39–1.25 %, P < 0.05), but N m contents were higher than in comparable studies. The relatively high N m and low LMA values are consistent with published global leaf trait datasets. No altitudinal trend for P m was found; rather, our data highlighted the spatial variability in P m (and P a) within and among sites at different elevations. Foliar N:P ratios did not show a trend with altitude and did not indicate N limitation except at 3000 m altitude.

Conclusions: Though leaf traits showed altitudinal trends similar to other studies, contrary to the general hypothesis, our data suggest that the tropical montane forests presented here are not N limited.  相似文献   

5.
Knowledge of nutrient storage and partitioning in forests is imperative for ecosystem models and ecological theory. Whether the nutrients (N, P, K, Ca, and Mg) stored in forest biomass and their partitioning patterns vary systematically across climatic gradients remains unknown. Here, we explored the global‐scale patterns of nutrient density and partitioning using a newly compiled dataset including 372 forest stands. We found that temperature and precipitation were key factors driving the nutrients stored in living biomass of forests at global scale. The N, K, and Mg stored in living biomass tended to be greater in increasingly warm climates. The mean biomass N density was 577.0, 530.4, 513.2, and 336.7 kg/ha for tropical, subtropical, temperate, and boreal forests, respectively. Around 76% of the variation in biomass N density could be accounted by the empirical model combining biomass density, phylogeny (i.e., angiosperm, gymnosperm), and the interaction of mean annual temperature and precipitation. Climate, stand age, and biomass density significantly affected nutrients partitioning at forest community level. The fractional distribution of nutrients to roots decreased significantly with temperature, suggesting that forests in cold climates allocate greater nutrients to roots. Gymnosperm forests tended to allocate more nutrients to leaves as compared with angiosperm forests, whereas the angiosperm forests distributed more nutrients in stems. The nutrient‐based Root:Shoot ratios (R:S), averaged 0.30 for R:SN, 0.36 for R:SP, 0.32 for R:SK, 0.27 for R:SCa, and 0.35 for R:SMg, respectively. The scaling exponents of the relationships describing root nutrients as a function of shoot nutrients were more than 1.0, suggesting that as nutrient allocated to shoot increases, nutrient allocated to roots increases faster than linearly with nutrient in shoot. Soil type significantly affected the total N, P, K, Ca, and Mg stored in living biomass of forests, and the Acrisols group displayed the lowest P, K, Ca, and Mg.  相似文献   

6.
Vast areas of southern Chile are now covered by second-growth forests because of fire and logging. To study successional patterns after moderate-intensity, anthropogenic fire disturbance, we assessed differences in soil properties and N fluxes across a chronosequence of seven successional stands (2–130 years old). We examined current predictions of successional theory concerning changes in the N cycle in forest ecosystems. Seasonal fluctuations of net N mineralization (Nmin) in surface soil and N availability (Na; Na=NH 4 + –N+NO 3 –N) in upper and deep soil horizons were positively correlated with monthly precipitation. In accordance with theoretical predictions, stand age was positively, but weakly related to both Na (r 2=0.282, P<0.001) and total N (Ntot; r 2=0.192, P<0.01), and negatively related to soil C/N ratios (r 2=0.187, P<0.01) in surface soils. A weak linear increase in soil Nmin (upper plus deep soil horizons) was found across the chronosequence (r 2=0.124, P<0.022). Nmin occurred at modest rates in early successional stands, suggesting that soil disturbance did not impair microbial processes. The relationship between N fixation (Nfix) in the litter layer and stand age best fitted a quadratic model (r 2=0.228, P<0.01). In contrast to documented successional trends for most temperate, tropical and Mediterranean forests, non-symbiotic Nfix in the litter layer is a steady N input to unpolluted southern temperate forests during mid and late succession, which may compensate for hydrological losses of organic N from old-growth ecosystems.  相似文献   

7.
Converting deciduous forests to coniferous plantations and vice versa causes environmental changes, but till now insight into the overall effect is lacking. This review, based on 38 case studies, aims to find out how coniferous and deciduous forests differ in terms of throughfall (+stemflow) deposition and seepage flux to groundwater. From the comparison of coniferous and deciduous stands at comparable sites, it can be inferred that deciduous forests receive less N and S via throughfall (+stemflow) deposition on the forest floor. In regions with relatively low open field deposition of atmospheric N (<10 kg N ha−1 year−1), lower NH4+ mean throughfall (+stemflow) deposition was, however, reported under conifers compared to deciduous forest, while in regions with high atmospheric N pollution (>10 kg N ha−1 year−1), the opposite could be concluded. The higher the open field deposition of NH4+, the bigger the difference between the coniferous and deciduous throughfall (+stemflow) deposition. Furthermore, it can be concluded that canopy exchange of K+, Ca2+ and Mg2+ is on average higher in deciduous stands. The significantly higher stand deposition flux of N and S in coniferous forests is reflected in a higher soil seepage flux of NO3, SO42−, K+, Ca2+, Mg2+ and Al(III). Considering a subset of papers for which all necessary data were available, a close relationship between throughfall (+stemflow) deposition and seepage was found for N, irrespective of the forest type, while this was not the case for S. This review shows that the higher input flux of N and S in coniferous forests clearly involves a higher seepage of NO3 and SO42− and accompanying cations K+, Ca2+, Mg2+ and Al(III) into the groundwater, making this forest type more vulnerable to acidification and eutrophication compared to the deciduous forest type.  相似文献   

8.
The Yungas, a system of tropical and subtropical montane forests on the eastern slopes of the Andes, are extremely diverse and severely threatened by anthropogenic pressure and climate change. Previous mycological works focused on macrofungi (e.g. agarics, polypores) and mycorrhizae in Alnus acuminata forests, while fungal diversity in other parts of the Yungas has remained mostly unexplored. We carried out Ion Torrent sequencing of ITS2 rDNA from soil samples taken at 24 sites along the entire latitudinal extent of the Yungas in Argentina. The sampled sites represent the three altitudinal forest types: the piedmont (400–700 m a.s.l.), montane (700–1500 m a.s.l.) and montane cloud (1500–3000 m a.s.l.) forests. The deep sequence data presented here (i.e. 4 108 126 quality‐filtered sequences) indicate that fungal community composition correlates most strongly with elevation, with many fungi showing preference for a certain altitudinal forest type. For example, ectomycorrhizal and root endophytic fungi were most diverse in the montane cloud forests, particularly at sites dominated by Alnus acuminata, while the diversity values of various saprobic groups were highest at lower elevations. Despite the strong altitudinal community turnover, fungal diversity was comparable across the different zonal forest types. Besides elevation, soil pH, N, P, and organic matter contents correlated with fungal community structure as well, although most of these variables were co‐correlated with elevation. Our data provide an unprecedented insight into the high diversity and spatial distribution of fungi in the Yungas forests.  相似文献   

9.
Global changes in nutrient deposition rates are likely to have profound effects on plant communities, particularly in the nutrient‐limited systems of the tropics. We studied the effects of increased nutrient availability on the seedlings of six tree species in montane forests of southern Ecuador in situ. After five years of continued N, P, or N+P addition, naturally grown seedlings of each of the two most common species at each elevation (1000, 2000, and 3000 m asl) were harvested for analyses of leaf morphology, nutrient content, herbivory, and tissue biomass allocation. Most species showed increased foliar N and P concentrations after addition of each respective element. Leaf tissue N:P ratios of >20 in the control plants of all species suggest that P is more growth‐limiting in these forests than N. Leaf morphological responses to nutrient addition were species and nutrient specific, with some species (Hedyosmum purparescens, Graffenrieda emarginata) exhibiting increased specific leaf area (SLA), and others (Graffenrieda harlingii) increased leaf area ratios (LAR). Pouteria torta (1000 m) had lower SLA and LAR after P addition. Increased herbivory was only evident in G. emarginata (after N and N+P addition). Only the species from 3000 m asl modified biomass allocation after nutrient addition. In general, N and N+P addition more strongly affected the species studied at the upper elevations, whereas P addition had a similar range of effects on the species at all elevations. We conclude that the responses of the studied tropical montane forest tree seedlings to chronic N and P addition are highly species‐specific and that successful adaptation to increased nutrient availability will depend on species‐specific morphological and physiological plasticity.  相似文献   

10.
Primary tropical forests generally exhibit large gaseous nitrogen (N) losses, occurring as nitric oxide (NO), nitrous oxide (N2O) or elemental nitrogen (N2). The release of N2O is of particular concern due to its high global warming potential and destruction of stratospheric ozone. Tropical forest soils are predicted to be among the largest natural sources of N2O; however, despite being the world’s second-largest rainforest, measurements of gaseous N-losses from forest soils of the Congo Basin are scarce. In addition, long-term studies investigating N2O fluxes from different forest ecosystem types (lowland and montane forests) are scarce. In this study we show that fluxes measured in the Congo Basin were lower than fluxes measured in the Neotropics, and in the tropical forests of Australia and South East Asia. In addition, we show that despite different climatic conditions, average annual N2O fluxes in the Congo Basin’s lowland forests (0.97 ± 0.53 kg N ha−1 year−1) were comparable to those in its montane forest (0.88 ± 0.97 kg N ha−1 year−1). Measurements of soil pore air N2O isotope data at multiple depths suggests that a microbial reduction of N2O to N2 within the soil may account for the observed low surface N2O fluxes and low soil pore N2O concentrations. The potential for microbial reduction is corroborated by a significant abundance and expression of the gene nosZ in soil samples from both study sites. Although isotopic and functional gene analyses indicate an enzymatic potential for complete denitrification, combined gaseous N-losses (N2O, N2) are unlikely to account for the missing N-sink in these forests. Other N-losses such as NO, N2 via Feammox or hydrological particulate organic nitrogen export could play an important role in soils of the Congo Basin and should be the focus of future research.Subject terms: Microbiology, Biogeochemistry  相似文献   

11.
Barron AR  Purves DW  Hedin LO 《Oecologia》2011,165(2):511-520
Symbiotic dinitrogen (N2) fixation is often invoked to explain the N richness of tropical forests as ostensibly N2-fixing trees can be a major component of the community. Such arguments assume N2 fixers are fixing N when present. However, in laboratory experiments, legumes consistently reduce N2 fixation in response to increased soil N availability. These contrasting views of N2 fixation as either obligate or facultative have drastically different implications for the N cycle of tropical forests. We tested these models by directly measuring N2-fixing root nodules and nitrogenase activity of individual canopy-dominant legume trees (Inga sp.) across several lowland forest types. Fixation was substantial in disturbed forests and some gaps but near zero in the high N soils of mature forest. Our findings suggest that canopy legumes closely regulate N2 fixation, leading to large variations in N inputs across the landscape, and low symbiotic fixation in mature forests despite abundant legumes.  相似文献   

12.
Microbial oxidation in aerobic soils is the primary biotic sink for atmospheric methane (CH4), a powerful greenhouse gas. Although tropical forest soils are estimated to globally account for about 28% of annual soil CH4 consumption (6.2 Tg CH4 year?1), limited data are available on CH4 exchange from tropical montane forests. We present the results of an extensive study on CH4 exchange from tropical montane forest soils along an elevation gradient (1,000, 2,000, 3,000 m) at different topographic positions (lower slope, mid-slope, ridge position) in southern Ecuador. All soils were net atmospheric CH4 sinks, with decreasing annual uptake rates from 5.9 kg CH4–C ha?1 year?1 at 1,000 m to 0.6 kg CH4–C ha?1 year?1 at 3,000 m. Topography had no effect on soil atmospheric CH4 uptake. We detected some unexpected factors controlling net methane fluxes: positive correlations between CH4 uptake rates, mineral nitrogen content of the mineral soil and with CO2 emissions indicated that the largest CH4 uptake corresponded with favorable conditions for microbial activity. Furthermore, we found indications that CH4 uptake was N limited instead of inhibited by NH4 +. Finally, we showed that in contrast to temperate regions, substantial high affinity methane oxidation occurred in the thick organic layers which can influence the CH4 budget of these tropical montane forest soils. Inclusion of elevation as a co-variable will improve regional estimates of methane exchange in these tropical montane forests.  相似文献   

13.
Litter production and N and P return were determined at bimonthly intervals for two years in 10×5 m plots, amended with a complete factorial fertilizer addition of N as NH4NO3(Na), P as Ca3(PO4)2(Pa) and a mixture of all essential nutrients excluding N and P (Ma) in a 4–7-year-old post-fire sand-plain lowland fynbos ecosystem, South Africa. Litter production increased with vegetation age, was highly seasonal and peaked from late spring to mid-summer (November to January). No significant differences in annual litter production and N return were found in response to the nutrient treatments, although both tended to increase during the second year in response to Na and Ma. Phosphorus return increased significantly with Pa, and to a lesser extend, N3, during the first year, whereas it increased in response to Na and Ma and decreased in the Pa amended plots during the second year. The nutrient treatments did not result in a change in the timing of the annual peak litter production period or in the plant growth form composition of the litter. The litter layer dry mass and N and P contents increased in response to Na and Ma, while Pa resulted in an increased P content. The evidence from this study indicates that the vegetative growth of the evergreen sclerophyllous shrubs and hemicryptophytes of sand-plain lowland fynbos is not only limited by N, as shown by other studies on shoot growth and vegetation cover, but also by one or more other nutrients excluding P.  相似文献   

14.
Paradoxically, symbiotic dinitrogen (N2) fixers are abundant in nitrogen (N)‐rich, phosphorus (P)‐poor lowland tropical rain forests. One hypothesis to explain this pattern states that N2 fixers have an advantage in acquiring soil P by producing more N‐rich enzymes (phosphatases) that mineralise organic P than non‐N2 fixers. We assessed soil and root phosphatase activity between fixers and non‐fixers in two lowland tropical rain forest sites, but also addressed the hypothesis that arbuscular mycorrhizal (AM) colonisation (another P acquisition strategy) is greater on fixers than non‐fixers. Root phosphatase activity and AM colonisation were higher for fixers than non‐fixers, and strong correlations between AM colonisation and N2 fixation at both sites suggest that the N–P interactions mediated by fixers may generally apply across tropical forests. We suggest that phosphatase enzymes and AM fungi enhance the capacity of N2 fixers to acquire soil P, thus contributing to their high abundance in tropical forests.  相似文献   

15.
Understanding how evolutionary constraints shape the elevational distributions of tree lineages provides valuable insight into the future of tropical montane forests under global change. With narrow elevational ranges, high taxonomic turnover, frequent habitat specialization, and exceptional levels of endemism, tropical montane forests and trees are predicted to be highly sensitive to environmental change. Using plot census data from a gradient traversing > 3,000 m in elevation on the Amazonian flank of the Peruvian Andes, we employ phylogenetic approaches to assess the influence of evolutionary heritage on distribution trends of trees at the genus‐level. We find that closely related lineages tend to occur at similar mean elevations, with sister genera pairs occurring a mean 254 m in elevation closer to each other than the mean elevational difference between non‐sister genera pairs. We also demonstrate phylogenetic clustering both above and below 1,750 m a.s.l, corresponding roughly to the cloud‐base ecotone. Belying these general trends, some lineages occur across many different elevations. However, these highly plastic lineages are not phylogenetically clustered. Overall, our findings suggest that tropical montane forests are home to unique tree lineage diversity, constrained by their evolutionary heritage and vulnerable to substantial losses under environmental changes, such as rising temperatures or an upward shift of the cloud‐base.  相似文献   

16.
Tropical montane forests are commonly limited by N or co-limited by N and P. Projected increases in N deposition in tropical montane regions are thought to be insufficient for vegetation demand and are not therefore expected to affect soil N availability and N2O emissions. We established a factorial N- and P-addition experiment (i.e., N, P, N + P, and control) across an elevation gradient of montane forests in Ecuador to test these hypotheses: (1) moderate rates of N and P additions are able to stimulate soil-N cycling rates and N2O fluxes, and (2) the magnitude and timing of soil N2O-flux responses depend on the initial nutrient status of the forest soils. Moderate rates of nutrients were added: 50 kg N ha?1 year?1 (in the form of urea) and 10 kg P ha?1 year?1 (in the form of NaH2PO 4 . 2H2O) split in two equal applications. We tested the hypotheses by measuring changes in net rates of soil–N cycling and N2O fluxes during the first 2 years (2008–2009) of nutrient manipulation in an old-growth premontane forest at 1,000 m, growing on a Cambisol soil with no organic layer, in an old-growth lower montane forest at 2,000 m, growing on a Cambisol soil with an organic layer, and an old-growth upper montane rainforest at 3,000 m, growing on a Histosol soil with a thick organic layer. Among the control plots, net nitrification rates were largest at the 1,000-m site whereas net nitrification was not detectable at the 2,000- and 3,000-m sites. The already large net nitrification at the 1,000-m site was not affected by nutrient additions, but net nitrification became detectable at the 2,000- and 3000-m sites after the second year of N and N + P additions. N2O emissions increased rapidly following N and N + P additions at the 1,000-m site whereas only smaller increases occurred at the 2,000- and 3,000-m sites during the second year of N and N + P additions. Addition of P alone had no effect on net rates of soil N cycling and N2O fluxes at any elevation. Our results showed that the initial soil N status, which may also be influenced by presence or absence of organic layer, soil moisture and temperature as encompassed by the elevation gradient, is a good indicator of how soil N cycling and N2O fluxes may respond to future increases in nutrient additions.  相似文献   

17.
Tropical nitrogen (N) deposition is projected to increase substantially within the coming decades. Increases in soil emissions of the climate‐relevant trace gases NO and N2O are expected, but few studies address this possibility. We used N addition experiments to achieve N‐enriched conditions in contrasting montane and lowland forests and assessed changes in the timing and magnitude of soil N‐oxide emissions. We evaluated transitory effects, which occurred immediately after N addition, and long‐term effects measured at least 6 weeks after N addition. In the montane forest where stem growth was N limited, the first‐time N additions caused rapid increases in soil N‐oxide emissions. During the first 2 years of N addition, annual N‐oxide emissions were five times (transitory effect) and two times (long‐term effect) larger than controls. This contradicts the current assumption that N‐limited tropical montane forests will respond to N additions with only small and delayed increases in soil N‐oxide emissions. We attribute this fast and large response of soil N‐oxide emissions to the presence of an organic layer (a characteristic feature of this forest type) in which nitrification increased substantially following N addition. In the lowland forest where stem growth was neither N nor phosphorus (P) limited, the first‐time N additions caused only gradual and minimal increases in soil N‐oxide emissions. These first N additions were completed at the beginning of the wet season, and low soil water content may have limited nitrification. In contrast, the 9‐ and 10‐year N‐addition plots displayed instantaneous and large soil N‐oxide emissions. Annual N‐oxide emissions under chronic N addition were seven times (transitory effect) and four times (long‐term effect) larger than controls. Seasonal changes in soil water content also caused seasonal changes in soil N‐oxide emissions from the 9‐ and 10‐year N‐addition plots. This suggests that climate change scenarios, where rainfall quantity and seasonality change, will alter the relative importance of soil NO and N2O emissions from tropical forests exposed to elevated N deposition.  相似文献   

18.
The effect of metal ions on human activated Factor X (Factor Xa) hydrolysis of the chromogenic substrate benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide (S2222) was studied utilizing initial rate enzyme kinetics. The divalent metal ions Ca2+, Mn2+, and Mg2+ enhanced Factor Xa amidolytic activity with Km values of 30 μm, 20 μm, and 1.4 mm, respectively. Na+ activation of Factor Xa amidolytic activity was also found. The Km for Na+ activation was 0.31 m. Both the divalent metal ions and Na+ increased the affinity of Factor Xa for S2222 and had no effect on the maximal velocity of the reaction. Other monovalent cations were unable to activate Factor Xa. However, K+ was a competitive inhibitor of the Na+ activation (Ki = 0.14 m). Lanthanide ions inhibited Factor Xa amidolytic activity. Gd3+ inhibition of Factor Xa hydrolysis of S2222 was noncompetitive and had a Ki of 3 μm. The lanthanide ion inhibition could not be reversed by Ca2+ even when Ca2+ was present in a 1000-fold excess over its Km indicating nonidentity of the Factor Xa lanthanide and Ca2+ binding sites. It is concluded that the Factor Xa Ca2+ binding sites have characteristics different from those previously described for the Factor X molecule and that Mg2+, Na+, and K+ may be physiological regulators of Factor Xa activity.  相似文献   

19.
Regulation of the cardiac ryanodine receptor (RyR2) by intracellular Ca2+ and Mg2+ plays a key role in determining cardiac contraction and rhythmicity, but their role in regulating the human RyR2 remains poorly defined. The Ca2+- and Mg2+-dependent regulation of human RyR2 was recorded in artificial lipid bilayers in the presence of 2 mM ATP and compared with that in two commonly used animal models for RyR2 function (rat and sheep). Human RyR2 displayed cytoplasmic Ca2+ activation (Ka = 4 µM) and inhibition by cytoplasmic Mg2+ (Ki = 10 µM at 100 nM Ca2+) that was similar to RyR2 from rat and sheep obtained under the same experimental conditions. However, in the presence of 0.1 mM Ca2+, RyR2s from human were 3.5-fold less sensitive to cytoplasmic Mg2+ inhibition than those from sheep and rat. The Ka values for luminal Ca2+ activation were similar in the three species (35 µM for human, 12 µM for sheep, and 10 µM for rat). From the relationship between open probability and luminal [Ca2+], the peak open probability for the human RyR2 was approximately the same as that for sheep, and both were ∼10-fold greater than that for rat RyR2. Human RyR2 also showed the same sensitivity to luminal Mg2+ as that from sheep, whereas rat RyR2 was 10-fold more sensitive. In all species, modulation of RyR2 gating by luminal Ca2+ and Mg2+ only occurred when cytoplasmic [Ca2+] was <3 µM. The activation response of RyR2 to luminal and cytoplasmic Ca2+ was strongly dependent on the Mg2+ concentration. Addition of physiological levels (1 mM) of Mg2+ raised the Ka for cytoplasmic Ca2+ to 30 µM (human and sheep) or 90 µM (rat) and raised the Ka for luminal Ca2+ to ∼1 mM in all species. This is the first report of the regulation by Ca2+ and Mg2+ of native RyR2 receptor activity from healthy human hearts.  相似文献   

20.
Tropical forests are a significant global source of the greenhouse gas nitrous oxide (N2O). Predicted environmental changes for this biome highlight the need to understand how simultaneous changes in precipitation and labile carbon (C) availability may affect soil N2O production. We conducted a small‐scale throughfall and leaf litter manipulation in a lowland tropical forest in southwestern Costa Rica to test how potential changes in both water and litter derived labile C inputs to soils may alter N2O emissions. Experimentally reducing throughfall in this wet tropical forest significantly increased soil emissions of N2O, and our data suggest that at least part of this response was driven by an increase in the concentration of dissolved organic carbon [DOC] inputs delivered from litter to soil under the drier conditions. Furthermore, [DOC] was significantly correlated with N2O emissions across both throughfall and litterfall manipulation plots, despite the fact that native NO3? pools in this site were generally small. Our results highlight the importance of understanding not only the potential direct effects of changing precipitation on soil biogeochemistry, but also the indirect effects resulting from interactions between the hydrologic, C and N cycles. Finally, over all sampling events we observed lower mean N2O emissions (<1 ng N2O‐N cm?2 h?1) than reported for many other lowland tropical forests, perhaps reflecting a more general pattern of increasing relative N constraints to biological activity as one moves from drier to wetter portions of the lowland tropical forest biome.  相似文献   

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