共查询到20条相似文献,搜索用时 62 毫秒
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Carme Gubern Olivia Hurtado Rocío Rodríguez Jesús R Morales Víctor G Romera María A Moro Ignacio Lizasoain Joaquín Serena Judith Mallolas 《BMC molecular biology》2009,10(1):57
Background
Studies of gene expression in experimental cerebral ischaemia models can contribute to understanding the pathophysiology of brain ischaemia and to identifying prognostic markers and potential therapeutic targets. The normalization of relative qRT-PCR data using a suitable reference gene is a crucial prerequisite for obtaining reliable conclusions. No validated housekeeping genes have been reported for the relative quantification of the mRNA expression profile activated in in-vitro ischaemic conditions, whereas for the in-vivo model different reference genes have been used. 相似文献3.
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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes 总被引:45,自引:0,他引:45
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Vandesompele J De Preter K Pattyn F Poppe B Van Roy N De Paepe A Speleman F 《Genome biology》2002,3(7):research0034.1-research003411
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Matthias B Van Hiel Pieter Van Wielendaele Liesbet Temmerman Sofie Van Soest Kristel Vuerinckx Roger Huybrechts Vanden Jozef Broeck Gert Simonet 《BMC molecular biology》2009,10(1):56
Background
To obtain reliable quantitative RT-PCR data, normalization relative to stable housekeeping genes is required. However, in practice, expression levels of 'typical' housekeeping genes have been found to vary between tissues and under different experimental conditions. To date, validation studies of reference genes in insects are extremely rare and have never been performed in locusts. In this study, putative housekeeping genes were identified in the desert locust, Schistocerca gregaria and two different software programs (geNorm and Normfinder) were applied to assess the stability of thesegenes. 相似文献7.
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Background
Normalization of gene expression data refers to the comparison of expression values using reference standards that are consistent across all conditions of an experiment. In PCR studies, genes designated as "housekeeping genes" have been used as internal reference genes under the assumption that their expression is stable and independent of experimental conditions. However, verification of this assumption is rarely performed. Here we assess the use of gene microarray analysis to facilitate selection of internal reference sequences with higher expression stability across experimental conditions than can be expected using traditional selection methods. 相似文献10.
Manuel Pombo-Suarez Manuel Calaza Juan J Gomez-Reino Antonio Gonzalez 《BMC molecular biology》2008,9(1):17
Background
Assessment of gene expression is an important component of osteoarthritis (OA) research, greatly improved by the development of quantitative real-time PCR (qPCR). This technique requires normalization for precise results, yet no suitable reference genes have been identified in human articular cartilage. We have examined ten well-known reference genes to determine the most adequate for this application. 相似文献11.
Heleen Nailis Tom Coenye Filip Van Nieuwerburgh Dieter Deforce Hans J Nelis 《BMC molecular biology》2006,7(1):25-9
Background
Candida albicans biofilms are commonly found on indwelling medical devices. However, the molecular basis of biofilm formation and development is not completely understood. Expression analysis of genes potentially involved in these processes, such as the ALS (Agglutinine Like Sequence) gene family can be performed using quantitative PCR (qPCR). In the present study, we investigated the expression stability of eight housekeeping genes potentially useful as reference genes to study gene expression in Candida albicans (C. albicans) biofilms, using the geNorm Visual Basic Application (VBA) for Microsoft Excel. To validate our normalization strategies we determined differences in ALS1 and ALS3 expression levels between C. albicans biofilm cells and their planktonic counterparts. 相似文献12.
Selection of reference genes for gene expression studies in human neutrophils by real-time PCR 总被引:1,自引:0,他引:1
Background
Reference genes, which are often referred to housekeeping genes, are frequently used to normalize mRNA levels between different samples. However the expression level of these genes may vary among tissues or cells, and may change under certain circumstances. Thus the selection of reference gene(s) is critical for gene expression studies. For this purpose, 10 commonly used housekeeping genes were investigated in isolated human neutrophils. 相似文献13.
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Rohini Mehta Aybike Birerdinc Noreen Hossain Arian Afendy Vikas Chandhoke Zobair Younossi Ancha Baranova 《BMC molecular biology》2010,11(1):39
Background
Given the epidemic proportions of obesity worldwide and the concurrent prevalence of metabolic syndrome, there is an urgent need for better understanding the underlying mechanisms of metabolic syndrome, in particular, the gene expression differences which may participate in obesity, insulin resistance and the associated series of chronic liver conditions. Real-time PCR (qRT-PCR) is the standard method for studying changes in relative gene expression in different tissues and experimental conditions. However, variations in amount of starting material, enzymatic efficiency and presence of inhibitors can lead to quantification errors. Hence the need for accurate data normalization is vital. Among several known strategies for data normalization, the use of reference genes as an internal control is the most common approach. Recent studies have shown that both obesity and presence of insulin resistance influence an expression of commonly used reference genes in omental fat. In this study we validated candidate reference genes suitable for qRT-PCR profiling experiments using visceral adipose samples from obese and lean individuals. 相似文献18.
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