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1.
以我国363份栽培和野生大豆资源为材料, 对大豆胞囊线虫抗性候选基因(rhg1和Rhg4)的SNP位点(8个)进行遗传变异分析, 以期阐明野生和栽培大豆间遗传多样性及连锁不平衡水平差异。结果表明, 与野生大豆相比, 代表我国栽培大豆总体资源多样性的微核心种质及其补充材料的连锁不平衡水平较高(R2值为0.216)。在栽培大豆群体内, 基因内和基因间分别有100%和16.6%的SNP位点对连锁不平衡显著, 形成两个基因特异的连锁不平衡区间(Block)。在所有供试材料中共检测到单倍型46个, 野生大豆的单倍型数目(27)少于栽培大豆(31), 但单倍型多样性(0.916)稍高于栽培大豆(0.816)。单倍型大多数(67.4%)为群体所特有(31个), 其中15个为野生大豆特有单倍型。野生大豆的两个主要优势单倍型(Hap_10和Hap_11)在栽培大豆中的发生频率也明显下降, 推测野生大豆向栽培大豆进化过程中, 一方面形成了新的单倍型, 另一方面因为瓶颈效应部分单倍型的频率降低甚至消失。 相似文献
2.
Background
Soybean cyst nematode (SCN) is the most economically devastating pathogen of soybean. Two resistance loci, Rhg1 and Rhg4 primarily contribute resistance to SCN race 3 in soybean. Peking and PI 88788 are the two major sources of SCN resistance with Peking requiring both Rhg1 and Rhg4 alleles and PI 88788 only the Rhg1 allele. Although simple sequence repeat (SSR) markers have been reported for both loci, they are linked markers and limited to be applied in breeding programs due to accuracy, throughput and cost of detection methods. The objectives of this study were to develop robust functional marker assays for high-throughput selection of SCN resistance and to differentiate the sources of resistance.Results
Based on the genomic DNA sequences of 27 soybean lines with known SCN phenotypes, we have developed Kompetitive Allele Specific PCR (KASP) assays for two Single nucleotide polymorphisms (SNPs) from Glyma08g11490 for the selection of the Rhg4 resistance allele. Moreover, the genomic DNA of Glyma18g02590 at the Rhg1 locus from 11 soybean lines and cDNA of Forrest, Essex, Williams 82 and PI 88788 were fully sequenced. Pairwise sequence alignment revealed seven SNPs/insertion/deletions (InDels), five in the 6th exon and two in the last exon. Using the same 27 soybean lines, we identified one SNP that can be used to select the Rhg1 resistance allele and another SNP that can be employed to differentiate Peking and PI 88788-type resistance. These SNP markers have been validated and a strong correlation was observed between the SNP genotypes and reactions to SCN race 3 using a panel of 153 soybean lines, as well as a bi-parental population, F5–derived recombinant inbred lines (RILs) from G00-3213 x LG04-6000.Conclusions
Three functional SNP markers (two for Rhg1 locus and one for Rhg4 locus) were identified that could provide genotype information for the selection of SCN resistance and differentiate Peking from PI 88788 source for most germplasm lines. The robust KASP SNP marker assays were developed. In most contexts, use of one or two of these markers is sufficient for high-throughput marker-assisted selection of plants that will exhibit SCN resistance.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1531-3) contains supplementary material, which is available to authorized users. 相似文献3.
P. B. Cregan J. Mudge E. W. Fickus D. Danesh R. Denny N. D. Young 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(5):811-818
The soybean cyst nematode (SCN) (Heterodera glycines Inchinoe) is the most economically significant soybean pest. The principal strategy to reduce or eliminate damage from this
pest is the use of resistant cultivars. Identifying resistant segregants in a breeding program is a difficult and expensive
process which is complicated by the oligogenic nature of the resistance and genetic variability in the pathogen. Fortunately,
resistance at one SCN-resistance locus, rhg1, is generally accepted as a necessity for the development of resistant genotypes using any source of resistance and when challenged
by any SCN race. Thus, the development of SCN resistant cultivars would be expedited if an effective and rapid system were
available to identify breeding lines carrying a resistance allele at the rhg1 locus. In this study we report two simple sequence repeat (SSR) or microsatellite loci that cosegregate and map 0.4 cM from
rhg1. Allelic variation at the first of these loci, BARC-Satt309, distinguished most, if not all, SCN-susceptible genotypes from
those carrying resistance at rhg1 derived from the important SCN-resistance sources ’Peking’, PI 437654, and PI 90763. BARC-Satt309 was also effective in distinguishing
SCN resistance sources PI 88788 and PI 209332 from many, but not all, susceptible genotypes. BARC-Satt309 cannot be used in
marker-assisted selection in populations developed from typical southern US cultivars crossed with the important resistance
sources PI 88788 or PI 209332 because these genotypes all carry the identical allele at the BARC-Satt309 locus. A second SSR
locus, BARC-Sat_168, was developed from a bacterial artificial chromosome (BAC) clone that was identified using the primers
to BARC-Satt309. BARC-Sat_168 distinguished PI 88788 and PI 209332 from southern US cultivars such as ’Lee’, ’Bragg’ and ’Essex’.
Both BARC-Satt309 and BARC-Sat_168 were used to assay lines from SCN-susceptible×SCN-resistant crosses and proved to be highly
effective in identifying lines carrying rhg1 resistance from those carrying the allele for SCN susceptibility at the rhg1 locus.
Received: 5 November 1998 / Accepted: 3 February 1999 相似文献
4.
Genomic analysis of the rhg1 locus: candidate genes that underlie soybean resistance to the cyst nematode 总被引:1,自引:0,他引:1
Ruben E Jamai A Afzal J Njiti VN Triwitayakorn K Iqbal MJ Yaegashi S Bashir R Kazi S Arelli P Town CD Ishihara H Meksem K Lightfoot DA 《Molecular genetics and genomics : MGG》2006,276(6):503-516
The rhg1 gene or genes lie at a recessive or co-dominant locus, necessary for resistance to all Hg types of the soybean (Glycine max (L.) Merr.) cyst nematode (Heterodera glycines I.). The aim here was to identify nucleotide changes within a candidate gene found at the rhg1 locus that were capable of altering resistance to Hg types 0 (race 3). A 1.5 ± 0.25 cM region of chromosome 18 (linkage group G) was shown to encompass rhg1 using recombination events from four near isogenic line populations and nine DNA markers. The DNA markers anchored two bacterial artificial chromosome (BAC) clones 21d9 and 73p6. A single receptor like kinase (RLK; leucine rich repeat-transmembrane-protein kinase) candidate resistance gene was amplified from both BACs using redundant primers. The DNA sequence showed nine alleles of the RLK at Rhg1 in the soybean germplasm. Markers designed to detect alleles showed perfect association between allele 1 and resistance to soybean cyst nematode Hg types 0 in three segregating populations, fifteen additional selected recombination events and twenty-two Plant Introductions. A quantitative trait nucleotide in the RLK at rhg1 was inferred that alters A47 to V47 in the context of H297 rather than N297. Contiguous DNA sequence of 315 kbp of chromosome 18 (about 2 cM) contained additional gene candidates that may modulate resistance to other Hg-types including a variant laccase, a hydrogen-sodium ion antiport and two proteins of unknown function. A molecular basis for recessive and co-dominant resistance that involves interactions among paralagous disease-resistance genes was inferred that would improve methods for developing new nematode-resistant soybean cultivars.Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. 相似文献
5.
6.
Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode 总被引:1,自引:0,他引:1
Jingyu Lin Mitra Mazarei Nan Zhao Junwei J. Zhu Xiaofeng Zhuang Wusheng Liu Vincent R. Pantalone Prakash R. Arelli Charles N. Stewart Jr Feng Chen 《Plant biotechnology journal》2013,11(9):1135-1145
Salicylic acid plays a critical role in activating plant defence responses after pathogen attack. Salicylic acid methyltransferase (SAMT) modulates the level of salicylic acid by converting salicylic acid to methyl salicylate. Here, we report that a SAMT gene from soybean (GmSAMT1) plays a role in soybean defence against soybean cyst nematode (Heterodera glycines Ichinohe, SCN). GmSAMT1 was identified as a candidate SCN defence‐related gene in our previous analysis of soybean defence against SCN using GeneChip microarray experiments. The current study started with the isolation of the full‐length cDNAs of GmSAMT1 from a SCN‐resistant soybean line and from a SCN‐susceptible soybean line. The two cDNAs encode proteins of identical sequences. The GmSAMT1 cDNA was expressed in Escherichia coli. Using in vitro enzyme assays, E. coli‐expressed GmSAMT1 was confirmed to function as salicylic acid methyltransferase. The apparent Km value of GmSAMT1 for salicylic acid was approximately 46 μm . To determine the role of GmSAMT1 in soybean defence against SCN, transgenic hairy roots overexpressing GmSAMT1 were produced and tested for SCN resistance. Overexpression of GmSAMT1 in SCN‐susceptible backgrounds significantly reduced the development of SCN, indicating that overexpression of GmSAMT1 in the transgenic hairy root system could confer resistance to SCN. Overexpression of GmSAMT1 in transgenic hairy roots was also found to affect the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction. 相似文献
7.
8.
Savithiry Natarajan Mona Tavakolan Nadim W Alkharouf Benjamin F Matthews 《Bioinformation》2014,10(6):387-389
Soybean cyst nematode (Heterodera glycines, SCN) is the most destructive pathogen of soybean around the world. Crop rotation and
resistant cultivars are used to mitigate the damage of SCN, but these approaches are not completely successful because of the
varied SCN populations. Thus, the limitations of these practices with soybean dictate investigation of other avenues of protection
of soybean against SCN, perhaps through genetically engineering of broad resistance to SCN. For better understanding of the
consequences of genetic manipulation, elucidation of SCN protein composition at the subunit level is necessary. We have
conducted studies to determine the composition of SCN proteins using a proteomics approach in our laboratory using twodimensional
polyacrylamide gel electrophoresis (2D-PAGE) to separate SCN proteins and to characterize the proteins further using
mass spectrometry. Our analysis resulted in the identification of several hundred proteins. In this investigation, we developed a
web based database (SCNProDB) containing protein information obtained from our previous published studies. This database will
be useful to scientists who wish to develop SCN resistant soybean varieties through genetic manipulation and breeding efforts.
The database is freely accessible from:
http://bioinformatics.towson.edu/Soybean_SCN_proteins_2D_Gel_DB/Gel1.aspx 相似文献
9.
Loci underlying resistance to Race 3 of soybean cyst nematode in Glycine soja plant introduction 468916 总被引:6,自引:0,他引:6
D. Wang B. W. Diers P. R. Arelli R. C. Shoemaker 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):561-566
Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is an important soybean [Glycine max (L.) Merr.] pest in the U.S. and throughout the world. Genetic resistance is the primary method for controlling SCN and there
is a need to identify new resistance genes. Glycine soja Sieb. and Zucc. is the wild ancestor of domesticated soybean and is a potential source of new SCN resistance genes. The goal
of this research was to map quantitative trait loci (QTLs) that provide resistance to SCN Race 3 from the G. soja plant introduction (PI) 468916. Fifty seven F2-derived lines from a cross between the G. soja PI 468916 and the G. max experimental line A81-356022 were tested for resistance to an SCN population with a Race-3 phenotype. These lines were also
genotyped with 1,004 genetic markers and resistance genes were mapped by composite interval mapping with the computer program
QTL-Cartographer. In the F2 population, three significant (LOD > 3.0) QTLs were detected that explained from 5% to 27% of the variation for Race-3 resistance.
The two most significant QTLs identified in the F2 population were tested in a population of 100 BC1F2 plants developed by crossing A81-356022 to a line from the F2 population that carried the two resistance QTLs from G. soja. In the backcross population, both Race-3 resistance QTLs were significant, which confirms the existence of these QTLs. The
QTLs identified in this experiment map to positions where SCN resistance genes have not been previously identified, suggesting
that these are novel genes that could be useful for diversifying the resistance genes currently used in cultivar development.
Received: 7 August 2000 / Accepted: 4 December 2000 相似文献
10.
大豆抗胞囊线虫病种质rhg1和Rhg4位点的单核苷酸多态性(SNPs) 总被引:8,自引:0,他引:8
本研究以57份中美大豆抗胞囊线虫病种质资源为实验材料,利用基于检测微珠的单碱基延伸方法,对与大豆胞囊线虫病(SCN)抗性基因rhg1和Rhg4紧密连锁的SNPs进行分析,目的是阐明我国大豆抗性种质在这两个位点的SNPs等位变异分布频率,为中国大豆种质抗SCN资源的利用奠定基础。分析结果表明,SNPs的抗性等位基因与中国大豆种质综合抗性的关系比不同生理小种的抗性关系更为密切。在rhg1和Rhg4位点,美国的9份抗性种质中,有7份抗性种质的SNPs均为纯合抗病基因型,而中国48份抗性种质中有32份。分别占鏊定总数的77.8%和66.7%,推测大豆抗SCN种质中,以rhg1和Rhg4这两个基因协同作用表现出的抗性可能占多数.但还存在其他的抗性机制。 相似文献
11.
V. D. Luedders 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1989,77(6):893-898
Summary Soybean (S, Glycine max (L.) Merr.) lines with relatively few cysts of soybean cyst nematode (CN, Heterodera glycines Ichinohe) populations are usually called CN-resistant. The phenotype of number of cysts per plant is of the CN-S (Cyst Nematode-Soybean) association and determined by the interactions of genes for avirulence-resistance. The acronym alins was proposed for these alleles for incompatibility, with xalin representing the interaction X of one microsymbiont malin with its host h-alin. These alins are dominant in the gene-for-gene model but may be mostly recessive with CN-S. Definitive genetic studies have been hindered by the heterogeneity of sexually reproducing CN populations and lack of the appropriate genetic models. Loegering's abstract interorganismal genetic model was modified so that one model represented all four possible interactions of dominant-recessive alins for an incompatible phenotype. This involved redefining the Boolean algebra symbol 1 to represent both the alins AND their frequencies. The model was used to derive the relationship: {ie893-01} where the expectation E of cysts (of any CN-S combination, as proportion of number of cysts on a check cultivar) is proportional to the product of CN genotypic frequencies expressed as functions of m-alin frequencies. Each m-alin is at a different locus, i.e., {ie893-02}. The number of terms multiplied for each CN-S is equal to the number of alins in the S line (or F2 plant). There are too many unknowns in the equation to solve for any of them. The relationship does explain the continuous distributions of phenotypes that were nearly always observed. Basic genetic principles were used to concurrently derive the models and to obtain discontinuous distributions of numbers of cyst phenotypes in segregating generations due to one recessive alin in a CN-susceptible soybean line.Contribution from the Missouri Agricultural Experiment Station, Journal Series No. 9739 相似文献
12.
R. A. Vierling J. Faghihi V. R. Ferris J. M. Ferris 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(1):83-86
Soybean cyst nematode (SCN) is a major soybean yield-limiting pest. The present study was conducted to map broad-based SCN resistance loci from the cultivar Hartwig. Two-hundred F23 lines derived from the cross Williams 82 x Hartwig were screened with a fourth-generation SCN inbred and 56 polymorphic molecular markers. Allele states and phenotypes were analyzed using stepwise regression and the model selection was made at P 0.01. Four unlinked RFLP markers (A006, A567, A487, A112) were associated with SCN resistance and the partial coefficient of determinations (R2) were 91%, 1%, 1%, and 1%. We have mapped a new, major SCN resistance locus (A006) and three minor loci (A567, A487, A112). This complete mapping will accelerate the transfer of broad-based resistance without linkage drag and aid in the determination of relationships among various SCN-resistant germplasm sources. 相似文献
13.
The National Academy of Agricultural Science (NAAS) has developed a web-based marker database to provide information about SNP markers in rice. The database consists of three major functional categories: map viewing, marker searching and gene annotation. It provides 12,829 SNP markers information including gene location information on 12 chromosomes in rice. The annotation of SNP marker provides information such as marker name, EST number, gene definition and general marker information. Users are assisted in tracing any new structures of the chromosomes and gene positional functions using specific SNP markers. AVAILABILITY: The database is available for free at http://nabic.niab.go.kr/SNP/ 相似文献
14.
B. F. Matthews M. H. MacDonald J. S. Gebhardt T. E. Devine 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1047-1052
The restriction fragment length polymorphism (RFLP) clone pBLT65 is a 450-nt soybean cDNA encoding a portion of the bifunctional
enzyme aspartokinase-homoserine dehydrogenase (AK-HSDH). pBLT65 maps within 3.5 cM of the i locus, conferring a pigmented seed coat, on linkage group A; hence, it is closely linked to the Rhg
4
locus conferring resistance to race 3 of the soybean cyst nematode. From this useful RFLP we developed a PCR reaction yielding
polymorphic bands for use in marker-assisted breeding programs to select progeny containing the Rhg
4
allele. The polymorphic bands were sequenced to determine the cause of the polymorphisms. Using primers 548 and 563, PCR
amplification of DNA from the soybean cultivar Peking (Rhg
4
) yielded three DNA fragments, 1a (1160 bp), 1b (1146 bp) and 3 (996 bp). Amplification of DNA from the cultivar Kent (rhg
4) yielded DNA fragments 2 (1020 bp), 3 (996 bp) and 4 (960 bp). Fragments 1a, 1b, 2 and 4 were also polymorphic between the
soybean lines PI 290136 and BARC-2(Rj
4
). A segregating population of 80 F2 and F3 plants derived from the cross PI 290136×BARC-2 (Rj
4
) was used to confirm the map position of the PCR polymorphisms near the i locus, and hence the Rhg
4
locus on linkage group A. The nucleotide sequences of fragments 1b, 3 and 4 were determined. Large and small deletions in
the intronic region were responsible for the size differences of the different fragments, whereas the exon was well conserved.
Received: 8 January 1998 / Accepted: 15 July 1998 相似文献
15.
Meksem Khalid Ruben Eliza Hyten David L. Schmidt Michael E. Lightfoot David A. 《Molecular breeding : new strategies in plant improvement》2001,7(1):63-71
An individual soybean breeder can generate over one hundred thousand new genotypes each year. The efficiency of selection in these populations could be improved if these genotypes were effectively screened with one DNA marker that identified an important gene, and if laboratory throughput was high and costs were low. Our aim was to develop a rapid genotyping procedure for resistance to the soybean cyst nematode. A high-throughput genotyping method was developed with fluorogenic probes to distinguish between two insertion polymorphisms in alleles of an AFLP marker that is located about 50 kbp from the Rhg4 gene candidate. The assay uses the 5 exonuclease activity of Taq polymerase in conjunction with fluorogenic probes for each allele. The method can be used for scoring the polymorphism in a recombinant inbred line population and for screening parent lines in a breeding program. The TaqmanTM method of determining genotype was accurate in 90% of scores in the RIL population compared to 95% accuracy with electrophoresis. Among 94 cultivars that are parents in our breeding program allele 2 that is derived from the sources of resistance to SCN was common in resistant cultivars (30 of 56) but rare in susceptible cultivars (3 of 38). Therefore, this method can be applied to automated large-scale genotyping for soybean breeding programs. 相似文献
16.
Yuan CP Li YH Liu ZX Guan RX Chang RZ Qiu LJ 《Molecular breeding : new strategies in plant improvement》2012,30(2):1155-1162
Rhg4 is one of the major resistant genes conferring resistance to soybean cyst nematode races 1, 3 and 4. In order to better understand its sequence diversity among different Chinese soybean populations and the impact of human activities on it, we designed 5 primer sets based on its sequence deposited in Genbank (Genbank accession No. AF506518) to obtain the Rhg4 sequence from 104 Chinese cultivated and wild soybean genotypes, and then analyzed the DNA sequence polymorphism in different Chinese soybean populations. The alignment of Rhg4 sequence included 5,216 nucleotide base pairs. A total of 67 single nucleotide polymorphisms (SNPs) including 59 single base changes and 8 DNA insertion-deletions (InDels) were identified with a SNP frequency of 1/78. Except for a 14-base InDel, there were 29 SNPs in coding regions, and among them, 13 were non-synonymous (9 in functional domains with 1 in a leucine-rich repeats region, 2 in a transmembrane region and 6 in a Ser/Thr kinase domain). The probability of substitution at each site was not the same, there were two hot spots, one was in the 5'-untranslated region between positions 124 and 804, and the other was in the region between positions 2520 and 3733. Sequence diversity analysis among 104 soybean genotypes showed π?=?0.00102 and θ?=?0.00218 for Rhg4. A domestication bottleneck was found because of lower sequence diversity and 58% unique SNPs loss in landraces compared with Glycine soja. Intensive selection increased the sequence diversity of cultivars, which had higher diversity and more unique SNPs than landraces. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-012-9703-1) contains supplementary material, which is available to authorized users. 相似文献
17.
Identification of a new major QTL associated with resistance to soybean cyst nematode (Heterodera glycines) 总被引:4,自引:0,他引:4
I. Schuster R. V. Abdelnoor S. R. R. Marin V. P. Carvalho R. A. S. Kiihl J. F. V. Silva C. S. Sediyama E. G. Barros M. A. Moreira 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(1):91-96
Resistance of soybean [Glycine max (L.) Merr.] to cyst nematode (SCN) (Heterodera glycines Ichinohe), one of the most destructive pathogens affecting soybean, involves a complex genetic system. The identification
of QTLs associated with SCN resistance may contribute to the understanding of such system. The objective of this work was
to identify and map QTLs for resistance to SCN Race 14 with the aid of molecular markers. BC3F2:3 and F2:3 populations, both derived from an original cross between resistant cv. Hartwig and the susceptible line BR-92–31983 were
screened for resistance to SCN Race 14. Four microsatellite (Satt082, Sat_001, Satt574 and Satt301) and four RAPD markers
(OPAA-11795, OPAE-08837, OPR-07548 and OPY-072030) were identified in the BC3F2:3 population using the bulked segregant analysis (BSA) technique. These markers were amplified in 183 F2:3 families and mapped to a locus that accounts for more than 40% of the resistance to SCN Race 14. Selection efficiency based
on these markers was similar to that obtained with the conventional method. In the case of the microsalellite markers, which
identify homozygous resistant genotypes, the efficiency was even higher. This new QTL has been mapped to the soybean linkage
group D2 and, in conjunction with other QTLs already identified for SCN resistance, will certainly contribute to our understanding
of the genetic basis of resistance of this important disease in soybean.
Received: 12 October 1999 / Accepted: 14 April 2000 相似文献
18.
Fendri-Kriaa N Boujilbene S Kammoun F Mkaouar-Rebai E Ben Mahmoud A Hsairi I Rebai A Triki C Fakhfakh F 《Biochemical and biophysical research communications》2011,408(4):654-657
Dravet syndrome (DS), previously known as severe myoclonic epilepsy of infancy, is one of the most severe forms of childhood epilepsy. DS is caused by a mutation in the neuronal voltage-gated sodium-channel alpha-subunit gene (SCN1A). However, 25–30% of patients with DS are negative for the SCN1A mutation screening, suggesting that other molecular mechanisms may account for these disorders. Recently, the first case of DS caused by a mutation in the neuronal voltage-gated sodium-channel beta-subunit gene (SCN1B) was also reported. In this report we aim to make the molecular analysis of the SCN1A and SCN1B genes in two Tunisian patients affected with DS. The SCN1A and SCN1B genes were tested for mutations by direct sequencing. No mutation was revealed in the SCN1A and SCN1B genes by sequencing analyses. On the other hand, 11 known single nucleotide polymorphisms were identified in the SCN1A gene and composed a putative disease-associated haplotype in patients with DS phenotype. One of the two patients with putative disease-associated haplotype in SCN1A had also one known single nucleotide polymorphism in the SCN1B gene. The sequencing analyses of the SCN1A gene revealed the presence of a putative disease-associated haplotype in two patients affected with Dravet syndrome. 相似文献
19.
The soybean Rhg1 amino acid transporter gene alters glutamate homeostasis and jasmonic acid-induced resistance to soybean cyst nematode 总被引:1,自引:0,他引:1
Wei Guo Feng Zhang Aili Bao Qingbo You Zeyu Li Jingsheng Chen Yihui Cheng Wei Zhao Xinjie Shen Xinan Zhou Yongqing Jiao 《Molecular Plant Pathology》2019,20(2):270-286
20.
Pyramiding multiple genes for resistance to soybean mosaic virus in soybean using molecular markers 总被引:1,自引:0,他引:1
Ainong Shi Pengyin Chen Dexiao Li Cuiming Zheng Bo Zhang Anfu Hou 《Molecular breeding : new strategies in plant improvement》2009,23(1):113-124
Seven strains of Soybean mosaic virus (SMV) and three independent resistance loci (Rsv1, Rsv3, and Rsv4) have been identified in soybean. The objective of this research was to pyramid Rsv1, Rsv3, and Rsv4 for SMV resistance using molecular markers. J05 carrying Rsv1 and Rsv3 and V94-5152 carrying Rsv4 were used as the donor parents for gene pyramiding. A series of F2:3, F3:4, and F4:5 lines derived from J05 × V94-5152 were developed for selecting individuals carrying all three genes. Eight PCR-based markers
linked to the three SMV resistance genes were used for marker-assisted selection. Two SSR markers (Sat_154 and Satt510) and
one gene-specific marker (Rsv1-f/r) were used for selecting plants containing Rsv1; Satt560 and Satt063 for Rsv3; and Satt266, AI856415, and AI856415-g for Rsv4. Five F4:5 lines were homozygous for all eight marker alleles and presumably carry all three SMV resistance genes that would potentially
provide multiple and durable resistance to SMV. 相似文献