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Gene expression of TPA induced differentiation in HL-60 cells by DNA microarray analysis 总被引:2,自引:0,他引:2
Zheng X Ravatn R Lin Y Shih WC Rabson A Strair R Huberman E Conney A Chin KV 《Nucleic acids research》2002,30(20):4489-4499
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S H Bernstein S M Kharbanda M L Sherman V P Sukhatme D W Kufe 《Cell growth & differentiation》1991,2(6):273-278
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Change in the level of CuZn-superoxide dismutase (SOD) mRNA was examined using a molecular probe during differentiation of human monocytic leukemia U937 cells or promyelotic leukemia HL-60 cells induced by either 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or dimethylsulfoxide (DMSO). CuZn-SOD mRNA levels were found to decrease during the course of differentiation, and this response is specific for differentiation, since the treatment of human B cell leukemia cells or normal diploid fibroblasts with TPA failed to have any effect on the level of CuZn-SOD mRNA. The activity of CuZn-SOD in U937 cells also decreased during differentiation, but following that of the CuZn-SOD mRNA level. The expression of the CuZn-SOD gene is thus concluded to diminish during the differentiation of HL-60 and U937 cells. 相似文献
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