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H2O2 intensifies CN−-induced apoptosis in pea leaves 总被引:1,自引:0,他引:1
Samuilov VD Kiselevsky DB Sinitsyn SV Shestak AA Lagunova EM Nesov AV 《Biochemistry. Biokhimii?a》2006,71(4):384-394
H2O2 intensifies CN(-)-induced apoptosis in stoma guard cells and to lesser degree in basic epidermal cells in peels of the lower epidermis isolated from pea leaves. The maximum effect of H2O2 on guard cells was observed at 10(-4) M. By switching on non-cyclic electron transfer in chloroplasts menadione and methyl viologen intensified H2O2 generation in the light, but prevented the CN--induced apoptosis in guard cells. The light stimulation of CN- effect on guard cell apoptosis cannot be caused by disturbance of the ribulose-1,5-bisphosphate carboxylase function and associated OH* generation in chloroplasts with participation of free transition metals in the Fenton or Haber-Weiss type reactions as well as with participation of the FeS clusters of the electron acceptor side of Photosystem I. Menadione and methyl viologen did not suppress the CN(-)-induced apoptosis in epidermal cells that, unlike guard cells, contain mitochondria only, but not chloroplasts. Quinacrine and diphenylene iodonium, inhibitors of NAD(P)H oxidase of cell plasma membrane, had no effect on the respiration and photosynthetic O2 evolution by leaf slices, but prevented the CN(-)-induced guard cell death. The data suggest that NAD(P)H oxidase of guard cell plasma membrane is a source of reactive oxygen species (ROS) needed for execution of CN(-)-induced programmed cell death. Chloroplasts and mitochondria were inefficient as ROS sources in the programmed death of guard cells. When ROS generation is insufficient, exogenous H2O2 exhibits a stimulating effect on programmed cell death. H2O2 decreased the inhibitory effects of DCMU and DNP-INT on the CN(-)-induced apoptosis of guard cells. Quinacrine, DCMU, and DNP-INT had no effect on CN(-)-induced death of epidermal cells. 相似文献
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Fabian Klaus Jörg Laufer Kamil Czarkowski Nathalie Strutz-Seebohm Guiscard Seebohm Florian Lang 《Biochemical and biophysical research communications》2009,381(3):407-411
The widely expressed chloride channel ClC-2 is stimulated by the serum and glucocorticoid inducible kinase SGK1. The SGK1-dependent regulation of several carriers involves the mammalian phosphatidylinositol-3-phosphate-5-kinase PIKfyve (PIP5K3). The present experiments explored whether SGK1-dependent regulation of ClC-2 similarly involves PIKfyve. The conductance of Xenopus oocytes is increased more than eightfold by ClC-2 expression. In ClC-2-expressing oocytes, but not in water-injected oocytes, the current was further enhanced by coexpression of either, PIKfyve or constitutively active S422DSGK1. Coexpression of the inactive SGK1 mutant K127NSGK1 did not significantly alter the current in ClC-2-expressing oocytes and abrogated the stimulation of the current by PIKfyve-coexpression. The stimulating effect of PIKfyve was abolished by replacement of the serine with alanine in the SGK1 consensus sequence (S318APIKfyve). Coexpression of S318APIKfyve significantly blunted the stimulating effect of S422DSGK1 on ClC-2-activity. In conclusion, PIKfyve is a potent stimulator of ClC-2-activity and contributes to SGK1-dependent regulation of ClC-2. 相似文献
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Rudolf Mierau Peter J. Robinson Arnold R. Sanderson Ekkehard Genth Matthias Cramer 《Immunogenetics》1987,26(6):351-355
The specificity of the mouse class I-specific antibody COB6-3 was examined in detail. It was found to react with the mouse class I molecules H-2Db, Kd, and Qa-2, and with human HLA-A, –B, –C antigens. The specificity pattern of COB6-3, despite its different origin, was similar to that of the monomorphic HLA class I-specific antibody W6/32. Cross-inhibition studies show that on human cells the antigenic determinants recognized by the two antibodies are situated close together and may be identical. On mouse cells, reactivity of both antibodies was generated upon replacement of mouse beta-2 microglobulin (B2m) with its bovine counterpart, but differences in specificity were observed using human B2m.Abbreviations used in this paper B2m
beta-2 microglobulin
- BSA
bovine serum albumin
- FCS
fetal calf serum
- FITC
fluorescein isothiocyanate
- MHC
major histocompatibility complex
- PBL
peripheral blood lymphocytes
- PBS
phosphate-buffered saline
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis 相似文献
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NO
2
–
efflux and its regulation have been studied in the cyanobacterium Nostoc MAC. 3-(3,4-Dichlorophenyl)-1,1-dimethylurea (DCMU), carbonyl cyanide-m-chlorophenyl hydrazone (CCCP), sodium azide, p-chloromercuribenzoate (PCMB), and dicyclohexylcarbodiimide (DCCD), a specific inhibitor of bacterial ATPase, inhibited the NO
2
–
efflux activity singificantly. No NO
2
–
efflux activity was observed under dark-aerobic as well as under dark-anaerobic conditions; however, the addition of ATP resulted in NO
2
–
efflux even under dark-aerobic condition. Maximum NO
2
–
efflux activity was observed when NO
3
–
served as the sole nitrogen source. However, NH
4
+
ions inhibited the NO
2
–
efflux activity when both NO
3
–
and NH
4
+
wer simulatneously available to the cells. The NO
2
–
efflux was freed from NH
4
+
repression by l-methionine-dl-sulfoximine (MSX), an irreversible inhibitor of glutamine synthetase (GS). Chloramphenicol, a protein synthesis inhibitor, inhibited the derepression of NO
2
–
efflux system when NH
4
+
-incubated cells were transferred to NO
3
–
medium. Tungstate-treated cells lacking functional NO
3
–
reductase but having NO
3
–
uptake activity also lacked NO
2
–
efflux activity. These results suggest that (i) NO
2
–
efflux in Nostoc MAC is NO
3
–
dependent and an energy-dependent process that can be regulated at the levels of NO
3
–
uptake and NO
3
–
reductase; (ii) NO
2
–
efflux system is NH
4
+
repressible; however, the product of NH
4
+
assimilation via GS is being required for repression to occur; (iii) de novo protein synthesis is required for derepression of the NO
2
–
efflux system; and (iv) the catalytic activity of NO
2
–
reductase also seems to play an important role in the regulation of NO
2
–
efflux system. 相似文献
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Grochala W 《Journal of molecular modeling》2007,13(6-7):757-767
The various spatial arrangements of frontier orbitals that may lead to facile reductive splitting of the H2 molecule at mono- or binuclear catalysts containing s, p, d or f-block metals, and on surfaces of solids are briefly reviewed. The postulation is also made that binuclear divalent titanium (Ti(II)) and mononuclear silicon (Si(II)) species might serve as active sites for the H2 attachment reaction for hydridoalanates doped with Ti salts and hydridoborates doped with SiO2, respectively. 相似文献
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《Inorganica chimica acta》1986,121(1):33-37
The known complex [Et4N]3[Fe(MoS4)2] has been shown by EPR and visible spectral studies to react with both thiophenol and selenophenol. The reaction results in a change in the characteristic S=3/2 EPR spectrum of this species from a complex rhombic pattern to one of a very simple axial appearance. Although this effect is similar to that observed for reaction of these species with the iron- molybdenum cofactor of nitrogenase, a moiety known to consist of a FeMoS cluster species, the large excesses of reagents and the long reaction times required for complete formation of product indicate that these reactions are of questionable direct relevance to the biological system. The reaction corresponding to the EPR spectral change from rhombic to axial in the [Fe(MoS4)2]3−/PhSeH system has also been partially characterized by product isolation which indicates that attack by selenol of the two terminal MoS2 moieties in the starting material has occurred. 相似文献
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《Inorganica chimica acta》1986,116(1):11-13
The emission spectra and excited state decay rates have been recorded for Cr(CN)6−n(H2O)nn−3 (n = 0-6) complexes. Both the transition energy and relaxation rates increase with n but the large changes in transition energies are not sufficient to account for the failure of the displaced coordinate to explain the relaxation rate results. 相似文献
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硒是哺乳动物不可缺少的微量元素,在人体内通过硒蛋白形式发挥多种生物学功能。早期的研究证实硒具有胰岛素样作用,补硒或硒蛋白可预防和治疗2型糖尿病(type 2 diabete mellitus, T2DM)。硒蛋白S (Selenoprotein S, SelS)参与机体内质网相关降解通路、氧化应激、炎症反应,并对血糖、血脂具有调控作用;同时,SelS异常表达于T2DM患者体内,参与胰岛素抵抗,并诱发血管病变。该文综述硒蛋白S基因的表达调控、生物学作用、代谢调节,以及与T2DM相关的研究,为T2DM的治疗提供理论依据。 相似文献
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<正>急性肺损伤是一种过度炎症反应,它是在感染、创伤等过程中导致的一种过度炎性反应,严重时则被称为急性呼吸窘迫综合征。多年来,急性肺损伤一直是临床常见的严重疾病,急性肺损伤的发病机制也未完全阐明。大量研究证明,继NO和CO之后的第3种信号分子H2S是重要的信使分子,并具有血管功能调节的作用。还有研究证明,H2S具有多方面的生物学作用,如参与低氧性肺动脉高压和感染性休克以及减轻兴奋性氨基酸对神经元的毒性作用等。内源性 相似文献