共查询到20条相似文献,搜索用时 31 毫秒
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Nakayama Y Nakajima Y Kato N Takai H Kim DS Arai M Mezawa M Araki S Sodek J Ogata Y 《Journal of cellular physiology》2006,208(2):326-335
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Samoto H Shimizu E Matsuda-Honjyo Y Saito R Nakao S Yamazaki M Furuyama S Sugiya H Sodek J Ogata Y 《The Journal of biological chemistry》2003,278(31):28659-28667
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Tumor necrosis factor-alpha (TNF-alpha) is a major mediator of inflammatory response in many diseases. It inhibits bone formation and stimulates bone resorption. To determine the molecular mechanisms involved in the regulation of gene expression of osteoblast-like cells, we analyzed the effects of TNF-alpha on the human osteosarcoma cell line Saos2. We used RT-PCR to examine the effects of TNF-alpha on bone sialoprotein (BSP), core binding factor a1 (Cbfa1), osterix, alpha 1 (I) collagen, cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), cathepsin B, cathepsin L and tissue inhibitors of metalloproteinase-1 (TIMP-1). TNF-alpha (10ng/ml) increased BSP, IL-6 and COX-2 mRNA levels after 3h, reaching maximal levels at 12 h. Cbfa1 mRNA levels increased after 3 h, but decreased by 24 h. Osterix, cathepsin B, cathepsin L and TIMP-1 mRNA levels did not change after stimulation with TNF-alpha. On the other hand, alpha 1 (I) collagen mRNA expression was suppressed by TNF-alpha at 24 h. Transient transfection analyses were performed using chimeric constructs of the rat BSP gene promoter linked to a luciferase reporter gene. TNF-alpha (10 ng/ml) had no effect on the promoter activities of BSP transfected into Saos2 cells. The results of gel mobility shift assays using radiolabeled double-stranded cAMP response element (CRE) and FGF2 response element (FRE) oligonucleotides in the proximal promoter of the rat BSP gene showed increased binding of nuclear proteins at 6 h. Gel mobility shift assays with radiolabelled COX-2-CRE and COX-2-NF kappa B oligonucleotides revealed an increase in the binding of nuclear proteins from TNF-alpha-stimulated Saos2 cells. These studies, therefore, showed that TNF-alpha indirectly increased BSP expression, and that it could be mediated through COX-2 and Cbfa1 expression in Saos2 osteoblast-like cells. 相似文献
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Sporophytic and gametophytic functions of the cell cycle-associated Mob1 gene in Arabidopsis thaliana L 总被引:1,自引:0,他引:1
Galla G Zenoni S Marconi G Marino G Botton A Pinosa F Citterio S Ruperti B Palme K Albertini E Pezzotti M Mau M Sharbel TF De Storme N Geelen D Barcaccia G 《Gene》2011,471(1-2):1-12
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Shimizu-Sasaki E Yamazaki M Furuyama S Sugiya H Sodek J Ogata Y 《The Journal of biological chemistry》2001,276(8):5459-5466
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Takai H Nakayama Y Kim DS Arai M Araki S Mezawa M Nakajima Y Kato N Masunaga H Ogata Y 《Journal of cellular biochemistry》2007,102(1):240-251