In vitro organogenesis and plant regeneration from leaves of Solanum candidum Lindl,S. quitoense Lam. (naranjilla) and S. sessiliflorum Dunal

Adventitious shoots and roots were regenerated from leaf segments of 3 Solanum species: S. candidum Lindl., S. quitoense Lam. and S. sessiliflorum Dunal. Leaf explants differentiated shoots on modified MS medium supplemented with 23–163 M kinetin and 0–5.7 µM indoleacetic acid (IAA). Excised shoots were induced to form roots by transfer to media with benzyladenine (BA) and naphthaleneacetic acid (NAA) at 0.09 and 0.11 µM respectively for S. quitoense and 0.01 µM NAA for S. candidum and S. sessiliflorum. Adventitious roots were produced directly from leaf explants with 0–140 µM kinetin and 0–5.7 µM IAA in combination. Rooted plants were successfully established in the greenhouse.     

ORIGINS OF SOLANUM LASIOCARPUM AND S. REPANDUM

Solanum candidum of tropical America is very similar to S. lasiocarpum of southeastern Asia and S. sessiliflorum, a wild and domesticated plant of the Amazon basin, is very similar to S. repandum, a weed and domesticated plant of Oceania. Artificial hybrids between S. candidum and S. lasiocarpum are fertile, and it is postulated that S. lasiocarpum resulted from a Spanish introduction of S. candidum to Asia. Hybrids between S. repandum and S. sessiliflorum show considerable reduction in fertility and abnormalities at meiosis, whereas hybrids of both S. candidum and S. lasiocarpum with S. repandum are fertile. It is concluded that it is likely that S. repandum did not arise from S. sessiliflorum but from S. lasiocarpum or S. candidum. This would mean that the similarity of S. repandum to S. sessiliflorum results from convergence.     

Solanum malacoxylon, a New Natural Host of Stolbur Phytoplasma

Stolbur phytoplasma infection has been reported, for the first time, in a new host, Solanum malacoxylon, growing in the Botanical Garden of Milan University. This shrub, native of South America, synthesizes vitamin D compounds, important for biomedical and biotechnological purposes. Pathogen detection was performed by light and transmission electron microscopy, and confirmed by molecular diagnosis, based on PCR and restriction fragment length polymorphic (RFLP) analysis of the phytoplasmal 16S rRNA and tuf genes. By means of enzymatic restriction and phylogenetic analysis on these genes, it was found that the phytoplasma belongs to the Stolbur group, taxonomic subgroup 16SrXII‐A, thus indicating S. malacoxylon as an additional host for this pathogen. Solanum malacoxylon could be then involved in the natural Stolbur phytoplasma spreading throughout South American areas, where this wild plant grows endemically.     

短梗千金藤，越南防己科新记录种

报道了越南防己科(Menispermaceae)一新记录种:短梗千金藤(Stephania brevipes Craib)。据文献记载,该种仅分布于泰国,现首次在越南发现其分布。本种与粪箕笃(S. longa Lour.)形态相近,但叶宽三角状卵形至三角状扁圆形,雄花序小,腋生或生于无叶的茎上,总梗较短,花萼淡黄色,花瓣红紫色,内果皮外部沟数目较少而与后者不同。还提供了该种详细的形态学描述、图版、分布及生态学等信息。凭证标本保存在越南国立自然博物馆标本馆(VNMN)和中国科学院华南植物园标本馆(IBSC)。     

中国大陆鸢尾兰属(兰科)二新记录种

首次报道了中国大陆兰科(Orchidaceae)鸢尾兰属(Oberonia)二新记录种:密花鸢尾兰[O. seidenfadenii (H. J. Su) Ormerod]和齿唇鸢尾兰(O. segawae T. C. Hsu & S. W. Chung),并提供了它们的形态特征描述。     

中国锦葵科一新记录种——克氏梧桐

首次报道中国锦葵科一新记录种——克氏梧桐［Firmiana kerrii (Craib) Kosterm.］。该种原记载产自泰国和缅甸,2019年该种在中国云南西双版纳州勐腊县发现有分布。凭证标本保存在中国科学院西双版纳热带植物园标本馆(HITBC)。该文给出了中国已知的9种梧桐属植物的分种检索表。     

中国植物区系新资料

该文报道了发现于云南盈江的2个中国植物新分布属——木瓜桐属（Siphonodon Griff.）和羽脉百部属（Stichoneuron Hook. f.）,以及对应的2个新记录种木瓜桐（Siphonodon celastrineus Griff.）和羽脉百部（Stichoneuron membranaceum Hook.f.）,并结合原始文献及野外调查对其形态特征进行了详细描述。凭证标本存放于中国科学院西双版纳热带植物园标本馆（HITBC）。     

A new species of <Emphasis Type="Italic">Brasiliomyces</Emphasis> (Erysiphaceae) on <Emphasis Type="Italic">Dalbergia cultrata</Emphasis> var. <Emphasis Type="Italic">cultrata</Emphasis> from Thailand

A powdery mildew fungus on leaves of Dalbergia cultrata var. cultrata (Fabaceae) collected at the Queen Sirikit Botanical Garden in northern Thailand is proven to be a new species of the genus Brasiliomyces and is described as B. chiangmaiensis sp. nov. with light and SEM micrographs. Differences in known Brasiliomyces species are discussed, and a key to species of this genus is provided.     

中国哥纳香属(番荔枝科)植物新资料

番荔枝科(Annonaceae)是基部被子植物木兰目(Magnoliales)中较进化且物种数最多的科。目前的系统发育研究将番荔枝科划分为4个亚科,即蒙蒿子亚科(Anaxagoreoideae)、澄光木亚科(Ambavioideae)、番荔枝亚科(Annonoideae)和排石木亚科(Malmeoideae),有107属,2 400多种,中国原产21 属约110 种。番荔枝科泛热带分布,是热带植物区系的优势类群,中国云南盈江位于云南省最西部边境,与缅甸东北部接壤,并与印度的东阿萨姆较近,植物区系处于东南亚(印度—马来西亚)热带生物区系向东亚亚热带-温带生物区系的过渡地带,属典型热带北缘性质,在植被地理和生物地理上十分重要,成为生物多样性保护的关键和热点地区。该区的热带雨林是印度阿萨姆和缅甸北部的热带雨林向东和向北扩散分布的边缘类型,是东南亚热带雨林在纬度和海拔分布上的极限类型。该文报道了采自中国云南省盈江县,引种保存于中国科学院西双版纳热带植物园的番荔枝科哥纳香属2个中国新记录种,即皱叶哥纳香 [Goniothalamus sesquipedalis(Colebr. ex Wall.)Hook. f. & Thomson]和长梗哥纳香(G. peduncularis King & Prain)。Flora of China将盈江哥纳香(G. lii X. L. Hou & Y. M. Shui)处理为云南哥纳香(G. yunnanensis W. T. Wang)的异名,基于活植物观察、馆藏标本和文献研究,该文对盈江哥纳香的分类地位进行了澄清,将其处理为长梗哥纳香的异名。皱叶哥纳香原记载产于印度、孟加拉国和缅甸等地,长梗哥纳香仅产于缅甸,该文对它们进行了补充描述,并提供彩色图版以便于鉴别。凭证标本存放于中国科学院西双版纳热带植物园标本馆(HITBC)。哥纳香属2个新记录的发现,丰富了中国番荔枝科植物多样性的认识,为中国云南热带植物区系属于热带亚洲(印度—马来西亚)植物区系,以及与缅甸北部、印度东北部植物区系的关系增加了例证。     

Ricerche Sulla Vegetazione Dell'etruria Marittima

Summary

The dates of foundation of the oldest university botanical gardens.

The dates of foundation of the oldest Botanical Gardens in Italy are not well known to the greatest part of the students of Botanical History.

Also today Ms. A. Guillaumin in his « Histoire de la Botanique en France (Paris, 1954) pag. 188» writes it was « Serenissima Repubblica Veneta » who founded the first Botanical Garden in 1533, whilst in that year only the chair of Bonafede had been constituded and the foundation of the Botanical Garden in Padoa happened later in 1545.

Really the most ancient Botanical Garden in the world is not that of Padoa, but that of Pisa.

Recently in the « Archivio di Stato » of Florence has been discovered by Fedeli a letter dated July 4 th 1545 written by Luca Ghini. From it it is clear that already a month before that date Luca Ghini was collecting plants to be coultivated in the academic garden of Pisa « for utility of students ».

The critical analysis of many papers by Luigi Anguillara allowe us to establish that Luca Ghini with the same Anguillara collected plants in the surreoundings of Pisa and on the Apuane Alps, from 1543 to 1544, and planted them in the academic garden of Pisa, which therefore must be considered the first Botanical Garden in the world, founded in the Summer 1543.

The second Botanical Garden in order of time is that of Padoa having been established by the « Senato Veneto » in 1545 and actually founded on July 7 th 1545 (de Visiani). The first director of Botanical Garden of Padoa was Luigi Anguillara after having left Pisa where he cooperated with Luca Ghini to found the Botanical Garden of Pisa.

Finally the third Botanical Garden is that of Florence (« Giardino dei Semplici ») founded as a dependance of that of Pisa by Luca Ghini, for school utility during the holyday, when the Florentine students remained at home. The date of its founding is December 1st 1545, as it appears from an an attorney document dated November 16th 1545 preserved in the « Archivio di Stato » of Florence (Volpi).

In conclusion the right chronology of the oldest University Botanical Gardens in the world is as follows:

1st: Pisa, Summer 1543

2nd: Padoa, July 7th 1545

3rd: Florence, December 1st 1545.     

中国石豆兰属(兰科)植物二新记录种北大核心CSCD

报道了在西藏自治区林芝市墨脱县发现的中国兰科石豆兰属二新记录种,即金氏石豆兰(Bulbophyllum kingii Hook. f.)和五脉石豆兰(Bulbophyllum pentaneurum Seidenf.)。该文对二者进行了描述,介绍了物候和分布信息,并提供彩色图片便于鉴别。凭证标本存放于中国科学院西双版纳热带植物园标本馆(HITBC)。     

Plant regeneration from the protoplasts of Solanum tuberosum, S. nigrum and S. bulbocastanum

The mesophyll protoplasts were isolated from the Solanum tuberosum (S. tbr) clones of different ploidy level (4x Bzura cv., 2x H-8105, and 2x ZEL-1136) as well as from the wild species: S. bulbocastanum (S. blb, 2x) and two accessions of S. nigrum (S. ngr, 6x). Additionally, the protoplasts were isolated from the cell suspensions of Bzura cv. and H-8105 clone. The conditions of protoplast isolation as well as the media for their culturing and regeneration, were selected and optimized for the studied genotypes. For mesophyll protoplasts, the shooting calli were produced by all the cultured protoclones except that of S. bulbocastanum. The shoots excised from the protoplast-derived calli developed into whole plants in all the studied potato clones but only in one accession of S. nigrum, i.e. S. ngr var. gigantea. As for suspension-cell-derived protoplasts, only H-8105 clone produced the regenerative type of calli, though normal shoots could not be obtained. The regenerative capacity of the protoplasts isolated from leaves and cell suspensions is compared and discussed. We regret to report the death of M. Sc. Maria Borkowska after the completion of this work.     

灰岩山姜，中国山姜属(姜科)一新记录种

报道了中国姜科山姜属植物1新记录种:灰岩山姜(Alpinia calcicola),并提供形态特征描述、地理分布及彩色图片。凭证标本保存在中国科学院华南植物园标本馆(IBSC)。     

中国球兰属一新记录

报道了夹竹桃科萝藦亚科（Asclepiadoideae,Apocynaceae）球兰属（Hoya R.Brown）一新记录种——万荣球兰（H.vangviengiensis Rodda&Simonsson）,提供了该种的形态描述。并对球兰属中少数具有透明乳汁的种进行了概述。凭证标本存放于中国科学院华南植物园（IBSC）。     

中国被子植物2新记录种——团花密花藤和珠芽秋海棠

该文首次报道了发现于云南德宏州的2个中国新记录种—团花密花藤(Pycnarrhena pleniflora Miers ex Hook.f. Thomson)和珠芽秋海棠(Begonia gemmipara Hook.f. Thomson)。结合原始文献、模式标本及其野外调查对其形态特征进行补充描述,凭证标本保存在中国科学院西双版纳热带植物园标本馆(HITBC)。     

西藏兰科新记录

报道了兰科（Orchidaceae）高山铠兰［Corybas himalaicus (King & Pantl.) Schltr.］和山珊瑚（Galeola faberi Rolfe）在中国西藏自治区的分布新记录。高山铠兰形态上因唇瓣先端边缘齿蚀状,花瓣与侧萼片在基部贴生,长7～8 mm,距暗红色而区别于铠兰属其他种;山珊瑚形态上因萼片狭椭圆形,唇瓣上散生褶片状附属物,边缘不规则齿状而区别于山珊瑚属其他种。凭证标本存放于广西药用植物园标本馆（GXMG）。     

南方红豆杉迁地保护小种群适应性进化机制研究

为了解南方红豆杉迁地保护种群的适应性进化机制,以南京中山植物园内南方红豆杉迁地保护栽培种群和扩散的衍生种群为材料,以引种初始地江西野生种群为对照,对南方红豆杉群落的结构、遗传多样性及遗传结构进行分析和研究。结果表明,南方红豆杉野生种群和迁地保护种群均维持着较高水平的遗传多样性,其中野生种群遗传多样性稍高于迁地保护小种群,但迁地保护种群中南方红豆杉个体密度大于野生种群。同时,种群内的遗传变异所占比例大于种群间的。整体来看,南方红豆杉迁地保护种群还处于演替早期,存在不明显的奠基者效应,有进一步演替发展的可能。     

中国瓜馥木属(番荔枝科)一新记录种

报道了中国番荔枝科瓜馥木属(Annonaceae:Fissistigma)一新记录种——瘤果瓜馥木[F.thorelii(Pierre ex FinetGagnep.)Merr.]。该种在中国产于云南省红河县,凭证标本保存在西双版纳热带植物园标本馆(HITBC)。该种的主要特征是幼枝、花、果均被褐色粗短硬毛,且果实表面布满瘤状突起。     

Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid Solanum tuberosum and S. brevidens

Summary Many somatic fusion hybrids have been produced between a dihaploid potato Solanum tuberosum and the sexually-incompatible wild species S. brevidens using both chemical and electrical fusion techniques. S. brevidens was resistant to both potato leaf roll virus (PLRV) and potato virus Y (PVY), the viruses being either at low (PLRV) or undetectable (PVY) concentrations as determined by enzyme-linked immunosorbent assay (ELISA). The S. tuberosum parent was susceptible to both viruses. A wide range of resistance, expressed as a decrease in virus concentration to both viruses was found amongst fusion hybrids, four of which were especially resistant. The practicality of introducing virus resistance from S. brevidens into cultivated potatoes by somatic hybridisation is discussed.