共查询到20条相似文献,搜索用时 15 毫秒
1.
Perugini LD Murphy JP Marshall D Brown-Guedira G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(3):417-425
Powdery mildew is an important foliar disease in wheat, especially in areas with a cool or maritime climate. A dominant powdery
mildew resistance gene transferred to the hexaploid germplasm line NC99BGTAG11 from T. timopheevii subsp. armeniacum was mapped distally on the long arm of chromosome 7A. Differential reactions were observed between the resistance gene in
NC99BGTAG11 and the alleles of the Pm1 locus that is also located on chromosome arm 7AL. Observed segregation in F2:3 lines from the cross NC99BGTAG11 × Axminster (Pm1a) demonstrate that germplasm line NC99BGTAG11 carries a novel powdery mildew resistance gene, which is now designated as Pm37. This new gene is highly effective against all powdery mildew isolates tested so far. Analyses of the population with molecular
markers indicate that Pm37 is located 16 cM proximal to the Pm1 complex. Simple sequence repeat (SSR) markers Xgwm332 and Xwmc790 were located 0.5 cM proximal and distal, respectively, to Pm37. In order to identify new markers in the region, wheat expressed sequence tags (ESTs) located in the distal 10% of 7AL that
were orthologous to sequences from chromosome 6 of rice were targeted. The two new EST-derived STS markers were located distal
to Pm37 and one marker was closely linked to the Pm1a region. These new markers can be used in marker-assisted selection schemes to develop wheat cultivars with pyramids of powdery
mildew resistance genes, including combinations of Pm37 in coupling linkage with alleles of the Pm1 locus. 相似文献
2.
Huang Z Chen Y Yi B Xiao L Ma C Tu J Fu T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(1):113-118
The Brassica napus oilseed rape line, 7-7365AB, is a recessive epistatic genic male sterile (RGMS) two-type line system. The sterility is controlled
by two pairs of recessive duplicate genes (Bnms3 and Bnms4) and one pair of recessive epistatic inhibitor gene (Bnrf). Homozygosity at the Bnrf locus (Bnrfrf) inhibits the expression of the two recessive male sterility genes in homozygous Bnms3ms3ms4ms4 plants and produces a male fertile phenotype. This line has a good potential for heterosis utilization but it is difficult
to breed heterotic hybrids without molecular markers. To develop markers linked to the BnMs3 gene, amplified fragment length polymorphism (AFLP) technology was applied to screen the bulks of sterile and fertile individuals
selected randomly from a population of near-isogenic lines (NIL) consisting of 2,000 plants. From a survey of 1,024 primer
combinations, we identified 17 AFLP markers linked to the BnMs3 gene. By integrating the previous markers linked to the BnMs3 gene into the genetic map of the NIL population, two markers, EA01MC12 and EA09P06, were located on either side of the BnMs3 gene at a distance of 0.1 and 0.3 cM, respectively. In order to use the markers for male sterile line breeding, five AFLP
markers, P05MG05, P03MG04, P11MG02, P05MC11250, and EA09P06, were successfully converted into sequence characterized amplified region (SCAR) markers. Two of these, P06MG04
and sR12384, were subsequently mapped on to linkage group N19 using two doubled-haploid mapping populations available at our
laboratory derived from the crosses Tapidor × Ningyou7 and Quantum × No2127-17. The markers found in the present study should
improve our knowledge of recessive genic male sterility (RGMS), and accelerate the development of male sterile line breeding
and map-based cloning. 相似文献
3.
Prashant G. Golegaonkar Haydar Karaoglu Robert F. Park 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(7):1281-1288
An incompletely dominant gene conferring resistance to Puccinia hordei, Rph14, identified previously in an accession of Hordeum vulgare, confers resistance to all known pathotypes of P. hordei in Australia. Knowledge of the chromosomal location of Rph14 and the identification of DNA markers closely linked to it will facilitate combining it with other important leaf rust resistance
genes to achieve long lasting resistance. The inheritance of Rph14 was confirmed using 146 and 106 F3 lines derived from the crosses ‘Baudin’/‘PI 584760’ (Rph14) and ‘Ricardo’/‘PI 584760’ (Rph14), respectively. Bulk segregant analysis on DNA from the parental genotypes and resistant and susceptible DNA bulks using
DArT markers located Rph14 to the short arm of chromosome 2H. DArT marker bPb-1664 was identified as having the closest genetic association with Rph14. PCR based marker analysis identified a single SSR marker, Bmag692, linked closely to Rph14 at a map distance of 2.1 and 3.8 cm in the ‘Baudin’/‘PI 584760’and ‘Ricardo’/‘PI 584760’ populations, respectively. 相似文献
4.
Gurung S Mamidi S Bonman JM Jackson EW del Río LE Acevedo M Mergoum M Adhikari TB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(6):1029-1041
Tan spot, caused by Pyrenophora tritici-repentis, is a major foliar disease of wheat worldwide. Host plant resistance is the best strategy to manage this disease. Traditionally,
bi-parental mapping populations have been used to identify and map quantitative trait loci (QTL) affecting tan spot resistance
in wheat. The association mapping (AM) could be an alternative approach to identify QTL based on linkage disequilibrium (LD)
within a diverse germplasm set. In this study, we assessed resistance to P. tritici-repentis races 1 and 5 in 567 spring wheat landraces from the USDA-ARS National Small Grains Collection (NSGC). Using 832 diversity
array technology (DArT) markers, QTL for resistance to P. tritici-repentis races 1 and 5 were identified. A linear model with principal components suggests that at least seven and three DArT markers
were significantly associated with resistance to P. tritici-repentis races 1 and 5, respectively. The DArT markers associated with resistance to race 1 were detected on chromosomes 1D, 2A, 2B,
2D, 4A, 5B, and 7D and explained 1.3–3.1% of the phenotypic variance, while markers associated with resistance to race 5 were
distributed on 2D, 6A and 7D, and explained 2.2–5.9% of the phenotypic variance. Some of the genomic regions identified in
this study correspond to previously identified loci responsible for resistance to P. tritici-repentis, offering validation for our AM approach. Other regions identified were novel and could possess genes useful for resistance
breeding. Some DArT markers associated with resistance to race 1 also were localized in the same regions of wheat chromosomes
where QTL for resistance to yellow rust, leaf rust and powdery mildew, have been mapped previously. This study demonstrates
that AM can be a useful approach to identify and map novel genomic regions involved in resistance to P. tritici-repentis. 相似文献
5.
Burt C Nicholson P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(8):1387-1400
Introgressions into wheat from related species have been widely used as a source of agronomically beneficial traits. One such
example is the introduction of the potent eyespot resistance gene Pch1 from the wild relative Aegilops ventricosa onto chromosome 7DL of wheat. In common with genes carried on many other such introgressions, the use of Pch1 in commercial wheat varieties has been hindered by linkage drag with yield-limiting traits. Attempts to break this linkage
have been frustrated by a lack of co-dominant PCR markers suitable for identifying heterozygotes in F2 populations. We developed conserved orthologous sequence (COS) markers, utilising the Brachypodium distachyon (Brachypodium) genome sequence, to provide co-dominant markers in the Pch1 region. These were supplemented with previously developed sequence-tagged site (STS) markers and simple sequence repeat (SSR)
markers. Markers were applied to a panel of varieties and to a BC6 F2 population, segregating between wheat and Ae. ventricosa over the distal portion of 7DL, to identify recombinants in the region of Pch1. By exploiting co-linearity between wheat chromosome 7D, Brachypodium chromosome 1, rice chromosome 6 and sorghum chromosome
10, Pch1 was located to an interval between the flanking markers Xwg7S and Xcos7-9. Furthermore candidate gene regions were identified in Brachypodium (364 Kb), rice (178 Kb) and sorghum (315 Kb) as a prelude
to the map-based cloning of the gene. In addition, using homoeologue transferable markers, we obtained evidence that the eyespot
resistances Pch1 and Pch2 on chromosomes 7D and 7A, respectively, are potentially homoeoloci. It is anticipated that the COS marker methodology could
be used for the identification of recombinants in other introgressions into wheat from wild relatives. This would assist the
mapping of genes of interest and the breaking of deleterious linkages to enable greater use of these introgressions in commercial
varieties. 相似文献
6.
Yoko Yatabe Chie Tsutsumi Yumiko Hirayama Keigo Mori Noriaki Murakami Masahiro Kato 《Journal of plant research》2009,122(6):585-595
Rheophilous Osmunda lancea often hybridizes with a dryland ally, Osmunda japonica, to produce O. × intermedia, forming zonation in riverbanks and the adjacent dryland along flooding frequency clines. This study examined the genetic
structure of populations consisting of O. × intermedia and the two parental species by analyzing ten nuclear DNA markers [six cleaved amplified polymorphic sequence (CAPS) markers
and three simple sequence repeat (SSR) markers developed from an expressed sequence tag (EST) library, and the sequence of
the glyceraldehyde-3-phosphate dehydrogenase gene GapCp] and chloroplast DNA sequences. The results suggest that the nuclear genes of O. japonica and O. lancea are genetically differentiated despite shared polymorphism in their chloroplast DNA sequences. This discrepancy may be attributable
to natural selection and recent introgression, although it is not evident if introgression occurs between O. japonica and O. lancea in the examined populations. Our findings of putative F2 hybrids in O. × intermedia support its partial reproducibility, and also suggest that formation of later-generation hybrids generates morphological
variation in O. × intermedia. O. lancea plants collected from geographically distant localities were genetically very similar, and it is suggested that O. lancea originated monotopically. 相似文献
7.
Jerzy K. Kulski Atsuko Shigenari Takashi Shiina Kazuyoshi Hosomichi Makoto Yawata Hidetoshi Inoko 《Immunogenetics》2009,61(4):257-270
The study of the association of the Human Leukocyte Antigen (HLA) alleles and polymorphic retrotransposons such as Alu, HERV, and LTR at various loci within the Major Histocompatibility Complex allows for a better identification and stratification of disease associations and the origins of HLA haplotypes in different populations. This paper provides sequence and association data on two structurally polymorphic MER9-LTR retrotransposons that are located 54 kb apart and in close proximity to the multiallelic HLA-A gene involved in the regulation of the human immune system. Direct DNA sequencing and analysis of the PCR products identified DNA nucleotide variations between the MER9-LTR sequences at the two loci and their associations with HLA-A alleles as potential haplotype and evolutionary markers. All MER9-LTR sequences were haplotypic when associated with common HLA-A alleles. The number of SNP loci was 2.5 times greater for the solo LTR at the AK locus, which is located closer to the HLA-A gene than the solo or 3′ LTR at the HG locus. Our study shows that the nucleotide variations of the MER9-LTR DNA sequences are additional informative markers in fine mapping HLA-A genomic haplotypes for future population, evolutionary, and disease studies. 相似文献
8.
Xifeng Chen Jianwei Pan Jing Cheng Guanghuai Jiang Yang Jin Zhimin Gu Qian Qian Wenxue Zhai Bojun Ma 《Molecular breeding : new strategies in plant improvement》2009,24(4):387-395
Spotted leaf 5 (spl5), a lesion mimic mutant, was first identified in rice (Oryza sativa L.) japonica cv. Norin8 in 1978. This mutant exhibits spontaneous disease-like lesions in the absence of any pathogens and resistance
to rice blast and bacterial blight; however, the target gene has not yet been isolated. In the present study, we employed
a map-based cloning strategy to finely map the spl5 gene. In an initial mapping with 100 F2 individuals (spl5/spl5) derived from a cross between the spl5 mutant and indica cv. 93-11, the spl5 gene was located in a 3.3-cM region on chromosome 7 using six simple sequence repeat (SSR) markers. In a high-resolution
genetic mapping, two F2 populations with 3,149 individuals (spl5/spl5) were derived from two crosses between spl5 mutant and two indica cvs. 93-11 and Zhefu802 and six sequence-tagged site (STS) markers were newly developed. Finally, the spl5 gene was mapped to a region of 0.048 cM between two markers SSR7 and RM7121. One BAC/PAC contig map covering these markers’
loci and the spl5 gene was constructed through Pairwise BLAST analysis. Our bioinformatics analysis shows that the spl5 gene is located in the 80-kb region between two markers SSR7 and RM7121 with a high average ratio of physical to genetic
distance (1.67 Mb/cM) and eighteen candidate genes. The analysis of these candidate genes indicates that the spl5 gene represents a novel class of regulators controlling cell death and resistance response in plants. 相似文献
9.
Satish K Gutema Z Grenier C Rich PJ Ejeta G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(6):989-1003
Striga is a devastating parasitic weed in Africa and parts of Asia. Low Striga germination stimulant activity, a well-known resistance mechanism in sorghum, is controlled by a single recessive gene (lgs). Molecular markers linked to the lgs gene can accelerate development of Striga-resistant cultivars. Using a high density linkage map constructed with 367 markers (DArT and SSRs) and an in vitro assay
for germination stimulant activity towards Striga asiatica in 354 recombinant inbred lines derived from SRN39 (low stimulant) × Shanqui Red (high stimulant), we precisely tagged and
mapped the lgs gene on SBI-05 between two tightly linked microsatellite markers SB3344 and SB3352 at a distance of 0.5 and 1.5 cM, respectively.
The fine-mapped lgs region was delimited to a 5.8 cM interval with the closest three markers SB3344, SB3346 and SB3343 positioned at 0.5, 0.7
and 0.9 cM, respectively. We validated tightly linked markers in a set of 23 diverse sorghum accessions, most of which were
known to be Striga resistant, by genotyping and phenotyping for germination stimulant activity towards both S. asiatica and S.
hermonthica. The markers co-segregated with Striga germination stimulant activity in 21 of the 23 tested lines. The lgs locus similarly affected germination stimulant activity for both Striga species. The identified markers would be useful in marker-assisted selection for introgressing this trait into susceptible
sorghum cultivars. Examination of the sorghum genome sequence and comparative analysis with the rice genome suggests some
candidate genes in the fine-mapped region (400 kb) that may affect strigolactone biosynthesis or exudation. This work should
form a foundation for map-based cloning of the lgs gene and aid in elucidation of an exact mechanism for resistance based on low Striga germination stimulant activity. 相似文献
10.
Raghvendra Kumar Mishra Anil Kumar Swati Chaudhary Sushil Kumar 《Journal of genetics》2009,88(2):227-232
The multifoliate pinna (mfp) mutation alters the leaf-blade architecture of pea, such that simple tendril pinnae of distal domain are replaced by compound
pinna blades of tendrilled leaflets in mfp homozygotes. The MFP locus was mapped with reference to DNA markers using F2 and F2:5 RIL as mapping populations. Among 205 RAPD, 27 ISSR and 35 SSR markers that demonstrated polymorphism between the parents
of mapping populations, three RAPD markers were found linked to the MFP locus by bulk segregant analyses on mfp/mfp and MFP/MFP bulks assembled from the F2:5 population. The segregational analysis of mfp and 267 DNA markers on 96 F2 plants allowed placement of 26 DNA markers with reference to MFP on a linkage group. The existence of common markers on reference genetic maps and MFP linkage group developed here showed that MFP is located on linkage group IV of the consensus genetic map of pea. 相似文献
11.
Previous research has demonstrated that the thermo-sensitive genic male-sterile (TGMS) gene in rice was regulated by temperature.
TGMS rice is important to hybrid rice production because the application of the TGMS system in two-line breeding is cost-effective,
simple, efficient and overcomes the limitations of the cytoplasmic male sterility (CMS) system. AnnongS is the first discovered
and deeply studied TGMS rice line in China. Previous studies have suggested that AnnongS-1 and Y58S, two derivative TGMS lines
of AnnongS, were both controlled by a single recessive gene named tms5, which was genetically mapped on chromosome 2. In the current study, three populations (AnnongS-1 × Nanjing11, Y58S × Q611,
and Y58S × Guanghui122) were developed to investigate the tms5 gene molecular map. Analysis of recombination events of sterile samples, utilizing 125 probes covering the tms5 region, suggested that the tms5 gene was physically mapped to a 19 kb DNA fragment between two markers, 4039-1 and 4039-2, located on the BAC clone AP004039.
Following the construction of a physical map between the two markers, ONAC023, a member of the NAC (NAM-ATAF-CUC-related) gene family, was identified as the candidate of the tms5 gene. 相似文献
12.
Runli He Zhijian Chang Zujun Yang Zongying Yuan Haixian Zhan Xiaojun Zhang Jianxia Liu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(6):1173-1180
Powdery mildew resistance from Thinopyrum intermedium was introgressed into common wheat (Triticum aestivum L.). Genetic analysis of the F1, F2, F3 and BC1 populations from powdery mildew resistant line CH5025 revealed that resistance was controlled by a single dominant allele.
The gene responsible for powdery mildew resistance was mapped by the linkage analysis of a segregating F2 population. The resistance gene was linked to five co-dominant genomic SSR markers (Xcfd233, Xwmc41, Xbarc11, Xgwm539 and Xwmc175) and their most likely order was Xcfd233–Xwmc41–Pm43–Xbarc11–Xgwm539–Xwmc175 at 2.6, 2.3, 4.2, 3.5 and 7.0 cM, respectively. Using the Chinese Spring nullisomic-tetrasomic and ditelosomic lines, the
polymorphic markers and the resistance gene were assigned to chromosome 2DL. As no powdery mildew resistance gene was previously
assigned to chromosome 2DL, this new resistance gene was designated Pm43. Pm43, together with the identified closely linked markers, could be useful in marker-assisted selection for pyramiding powdery
mildew resistance genes.
Runli He and Zhijian Chang contributed equally to this work. 相似文献
13.
Kristin Simons Zewdie Abate Shiaoman Chao Wenjun Zhang Matt Rouse Yue Jin Elias Elias Jorge Dubcovsky 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(3):649-658
Wheat stem rust caused by Puccinia graminis f. sp. tritici, can cause significant yield losses. To combat the disease, breeders have deployed resistance genes both individually and
in combinations to increase resistance durability. A new race, TTKSK (Ug99), identified in Uganda in 1999 is virulent on most
of the resistance genes currently deployed, and is rapidly spreading to other regions of the world. It is therefore important
to identify, map, and deploy resistance genes that are still effective against TTKSK. One of these resistance genes, Sr13, was previously assigned to the long arm of chromosome 6A, but its precise map location was not known. In this study, the
genome location of Sr13 was determined in four tetraploid wheat (T. turgidum ssp. durum) mapping populations involving the TTKSK resistant varieties Kronos, Kofa, Medora and Sceptre. Our results showed that resistance
was linked to common molecular markers in all four populations, suggesting that these durum lines carry the same resistance
gene. Based on its chromosome location and infection types against different races of stem rust, this gene is postulated to
be Sr13. Sr13 was mapped within a 1.2–2.8 cM interval (depending on the mapping population) between EST markers CD926040 and BE471213, which corresponds to a 285-kb region in rice chromosome 2, and a 3.1-Mb region in Brachypodium chromosome 3. These maps will be the foundation for developing high-density maps, identifying diagnostic markers, and positional
cloning of Sr13. 相似文献
14.
15.
Hughes SL Hunter PJ Sharpe AG Kearsey MJ Lydiate DJ Walsh JA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(7):1169-1173
A new source of resistance to the pathotype 4 isolate of Turnip mosaic virus (TuMV) CDN 1 has been identified in Brassica napus (oilseed rape). Analysis of segregation of resistance to TuMV isolate CDN 1 in a backcross generation following a cross between a resistant and a susceptible B. napus line showed that the resistance was dominant and monogenic. Molecular markers linked to this dominant resistance were identified using amplified fragment length polymorphism (AFLP) and microsatellite bulk segregant analysis. Bulks consisted of individuals from a BC1 population with the resistant or the susceptible phenotype following challenge with CDN 1. One AFLP and six microsatellite markers were associated with the resistance locus, named TuRB03, and these mapped to the same region on chromosome N6 as a previously mapped TuMV resistance gene TuRB01. Further testing of TuRB03 with other TuMV isolates showed that it was not effective against all pathotype 4 isolates. It was effective against some, but not all pathotype 3 isolates tested. It provided further resolution of TuMV pathotypes by sub-dividing pathotypes 3 and 4. TuRB03 also provides a new source of resistance for combining with other resistances in our attempts to generate durable resistance to this virus. 相似文献
16.
Sybil A. Herrera-Foessel Evans S. Lagudah Julio Huerta-Espino Matthew J. Hayden Harbans S. Bariana Davinder Singh Ravi P. Singh 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(1):239-249
The common wheat genotype ‘RL6077’ was believed to carry the gene Lr34/Yr18 that confers slow-rusting adult plant resistance (APR) to leaf rust and stripe rust but located to a different chromosome
through inter-chromosomal reciprocal translocation. However, haplotyping using the cloned Lr34/Yr18 diagnostic marker and the complete sequencing of the gene indicated Lr34/Yr18 is absent in RL6077. We crossed RL6077 with the susceptible parent ‘Avocet’ and developed F3, F4 and F6 populations from photoperiod-insensitive F3 lines that were segregating for resistance to leaf rust and stripe rust. The populations were characterized for leaf rust
resistance at two Mexican sites, Cd. Obregon during the 2008–2009 and 2009–2010 crop seasons, and El Batan during 2009, and
for stripe rust resistance at Toluca, a third Mexican site, during 2009. The F3 population was also evaluated for stripe rust resistance at Cobbitty, Australia, during 2009. Most lines had correlated responses
to leaf rust and stripe rust, indicating that either the same gene, or closely linked genes, confers resistance to both diseases.
Molecular mapping using microsatellites led to the identification of five markers (Xgwm165, Xgwm192, Xcfd71, Xbarc98 and Xcfd23) on chromosome 4DL that are associated with this gene(s), with the closest markers being located at 0.4 cM. In a parallel
study in Canada using a Thatcher × RL6077 F3 population, the same leaf rust resistance gene was designated as Lr67 and mapped to the same chromosomal region. The pleiotropic, or closely linked, gene derived from RL6077 that conferred stripe
rust resistance in this study was designated as Yr46. The slow-rusting gene(s) Lr67/Yr46 can be utilized in combination with other slow-rusting genes to develop high levels of durable APR to leaf rust and stripe
rust in wheat. 相似文献
17.
Kang H Weng Y Yang Y Zhang Z Zhang S Mao Z Cheng G Gu X Huang S Xie B 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(4):795-803
Scab, caused by Cladosporium cucumerinum, is an important disease of cucumber, Cucumis sativus. In this study, we conducted fine genetic mapping of the single dominant scab resistance gene, Ccu, with 148 F9 recombinant inbred lines (RILs) and 1,944 F2 plants derived from the resistant cucumber inbred line 9110Gt and the susceptible line 9930, whose draft genome sequence
is now available. A framework linkage map was first constructed with simple sequence repeat markers placing Ccu into the terminal 670 kb region of cucumber Chromosome 2. The 9110Gt genome was sequenced at 5× genome coverage with the
Solexa next-generation sequencing technology. Sequence analysis of the assembled 9110Gt contigs and the Ccu region of the 9930 genome identified three insertion/deletion (Indel) markers, Indel01, Indel02, and Indel03 that were closely
linked with the Ccu locus. On the high-resolution map developed with the F2 population, the two closest flanking markers, Indel01 and Indel02, were 0.14 and 0.15 cM away from the target gene Ccu, respectively, and the physical distance between the two markers was approximately 140 kb. Detailed annotation of the 180 kb
region harboring the Ccu locus identified a cluster of six resistance gene analogs (RGAs) that belong to the nucleotide binding site (NBS) type R
genes. Four RGAs were in the region delimited by markers Indel01 and Indel02, and thus were possible candidates of Ccu. Comparative DNA analysis of this cucumber Ccu gene region with a melon (C. melo) bacterial artificial chromosome (BAC) clone revealed a high degree of micro-synteny and conservation of the RGA tandem repeats
in this region. 相似文献
18.
Fine mapping of a male sterility gene <Emphasis Type="Italic">MS-cd1</Emphasis> in <Emphasis Type="Italic">Brassica oleracea</Emphasis> 总被引:1,自引:0,他引:1
Zhang X Wu J Zhang H Ma Y Guo A Wang X 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(2):231-238
A dominant male sterility (DGMS) line 79-399-3, developed from a spontaneous mutation in Brassica oleracea var. capitata, has been widely used in production of hybrid cultivars in China. In this line, male sterility is controlled by a dominant
gene Ms-cd1. In the present study, fine mapping of Ms-cd1 was conducted by screening a segregating population Ms79-07 with 2,028 individuals developed by four times backcrossing using
a male sterile Brassica oleracea var. italica line harboring Ms-cd1 as donor and Brassica oleracea var. alboglabra as the recipient. Bulked segregation analysis (BSA) was performed for the BC4 population Ms79-07 using 26,417 SRAP primer SRAPs and 1,300 SSRs regarding of male sterility and fertility. A high-resolution
map surrounding Ms-cd1 was constructed with 14 SRAPs and one SSR. The SSR marker 8C0909 was closely linked to the MS-cd1 gene with a distance of 2.06 cM. Fourteen SRAPs closely linked to the target gene were identified; the closest ones on each
side were 0.18 cM and 2.16 cM from Ms-cd1. Three of these SRAPs were successfully converted to dominant SCAR markers with a distance to the Ms-cd1 gene of 0.18, 0.39 and 4.23 cM, respectively. BLAST analysis with these SCAR marker sequences identified a collinear genomic
region about 600 kb in scaffold 000010 on chromosomeA10 in B. rapa and on chromosome 5 in A. thaliana. These results provide additional information for map-based cloning of the Ms-cd1 gene and will be helpful for marker-assisted selection (MAS). 相似文献
19.
Xu J Wang B Wu Y Du P Wang J Wang M Yi C Gu M Liang G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(2):365-372
Photoperiod-thermo-sensitive genic male sterile (PTGMS) rice exhibits a number of desirable traits for hybrid rice production.
The cloning genes responsible for PTGMS and those elucidating male sterility mechanisms and reversibility to fertility would
be of great significance to provide a foundation to develop new male sterile lines. Guangzhan63S, a PTGMS line, is one of
the most widely used indica two-line hybrid rice breeding systems in China. In this study, genetic analysis based on F2 and BC1F2 populations derived from a cross between Guangzhan63S and 1587, determined a single recessive gene controls male sterility
in Guangzhan63S. Molecular marker techniques combined with bulked-segregant analysis (BSA) were used and located the target
gene (named ptgms2-1) between two SSR markers RM12521 and RM12823. Fine mapping of the ptgms2-1 locus was conducted with 45 new Insertion–Deletion (InDel) markers developed between the RM12521 and RM12823 region, using
634 sterile individuals from F2 and BC1F2 populations. Ptgms2-1 was further mapped to a 50.4 kb DNA fragment between two InDel markers, S2-40 and S2-44, with genetic distances of 0.08 and
0.16 cM, respectively, which cosegregated with S2-43 located on the AP004039 BAC clone. Ten genes were identified in this
region based on annotation results from the RiceGAAS system. A nuclear ribonuclease Z gene was identified as the candidate
for the ptgms2-1 gene. This result will facilitate cloning the ptgms2-1 gene. The tightly linked markers for the ptgms2-1 gene locus will further provide a useful tool for marker-assisted selection of this gene in rice breeding programs. 相似文献
20.
Ya Zhang Li Xu Deng-Feng Zhang Jing-Rui Dai Shou-Cai Wang 《Molecular breeding : new strategies in plant improvement》2010,25(3):433-439
Southern corn rust, caused by Puccinia polysora Underw., has destructive potential on the susceptible host. In this study, the resistance inheritance was investigated in
an F
2 and its F
2:3 populations derived from a cross from two inbred lines W2D (resistant) and W222 (susceptible). The 3:1 ratio of resistant
to susceptible plants indicated that the resistance is controlled by one dominant gene (named as RppD). The gene RppD was located by means of the F
2 population. Total of 11 markers, including five SSR markers, five sequence-tagged site markers and one cleaved-amplified
polymorphic sequence (CAPS) marker, were identified to narrow the gene RppD down to a smaller interval. The closest markers flanking RppD were SSR marker umc1291 and CAPS marker CAPS858, with genetic distances of 2.9 and 0.8 cM, respectively. Moreover, RppD might be a novel Rpp resistance gene or haplotype differing from RppQ and RppP25 according to an allelism test among the three crosses W2D × Qi319, W2D × P25 and Qi319 × P25. As a result, RppD haplotype might be helpful to maize germplasm enhancement and disease-resistant breeding. 相似文献