共查询到20条相似文献,搜索用时 15 毫秒
1.
Mu‐Gil Kwon Deuk‐Su Kim Jeong‐Hwan Lee Se‐Ra Park Junsik Ahn Joo‐Sung Kim Kinarm Ko Sungsu Park Young‐Sun Kang Young‐Kug Choo Kisung Ko 《Entomological Research》2013,43(2):101-107
A male silkworm pupae extract with herbal mixtures such as Rubus coreanus Miquel, Chinese matrimony vine Acanthopanax senticosus and tocopherol can be effectively used to recover or boost stamina. In this study, the effect of the male silkworm pupae extract on the reproductive system of Sprague–Dawley male rats was investigated. No clinical symptoms, and no dying or dead animals were found among experimental male silkworm larvae extract, male silkworm pupae extract and control rat groups during the study. No significant differences were found in body weights, feed or water consumption, or macroscopic examination among the three groups. No lesion was found upon necropsy. However, sperm in the group treated with male silkworm pupae extract were significantly more active than sperm in the control group. Sperm counts in both male groups treated with male silkworm larvae extract and male silkworm pupae extract were significantly higher than sperm counts in the control group. Analysis of phospholipid–hydroperoxide glutathione peroxidase (PHGPx) gene expression showed that the male silkworm pupae extract increased expression in testes by 26.7%. This study shows that the male silkworm pupae extract has potential to be used to enhance the function of the human reproductive system. 相似文献
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This study describes morphological changes in the male reproductive system of Miroculis amazonicus (Savage & Peters) from mature nymphs to subimago stages. The sperm ultrastructure of Massartela brieni (Lestage), Farrodes carioca (Domínguez et al) and Miroculis mourei (Savage & Peters), as well as aspects of cell fragments observed in these species' subimagos deferent ducts were described. Sperm from the three species studied are aflagellated and immotile, while those from F. carioca and Ma. brieni are approximately spherical with a homogenous nucleus and acrosome. Sperm of F. carioca present two or three mitochondria located between the nucleus and the acrosome. In Ma. brieni, only one lateral mitochondria was found. Sperm from Mi. mourei are shaped as a number 'eight', with electron lucent spots inside the nucleus and two mitochondria above the acrosome. Large cell fragments containing degenerative vesicles and some sperm were observed in the deferent duct lumen of the three species. Testes of Mi. amazonicus are extremely reduced in the subimago stage, which suggests that these cell fragments originated from testes degeneration. 相似文献
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Somatostatin-14 (SRIF-14) has been shown to occur throughout the male rat reproductive system by SRIF radioimmunoassay, Sephadex G-50 exclusion chromatography, and parallel line analysis. SRIF-28 was found only in the epididymis. The highest concentrations of total SRIF-like immunoreactivity (SLI), representing the combined concentrations of SRIF-14 and SRIF-28, were measured in the prostates of 1 1/2- and 3-month-old Sprague-Dawley rats. The levels of SLI in prostates from 9-month-old males were about 10% that of the younger animals. Dilution curves for extracts of all reproductive tissues were parallel with synthetic SRIF-14. 相似文献
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S. D. Kholkute Rohini P. Patil Meena P. Desai C. P. Puri 《Primates; journal of primatology》1988,29(4):549-555
Intramuscular administration of metachlopromide (2.5, 5, and 10 mg) induced a dose-dependent increase in plasma prolactin
levels. The magnitude and duration of metachlopromide-induced hyperprolactinemia were also dose related. However, metachlopromide
treatment (5 mg/day) for 60 days failed to affect ovarian function in the common marmoset as evidenced by ovulatory plasma
estradiol and progesterone profiles. During the pretreatment cycle, there was no consistent pattern in plasma prolactin levels
depending on the stage of cycle. During lactation, higher levels of plasma prolactin were observed. 相似文献
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Sulfated glycoprotein-1 (saposin precursor) in the reproductive tract of the male rat 总被引:1,自引:0,他引:1
Sulfated glycoprotein-1 is one of the major protein secretion products of rat Sertoli cells in culture. This 70,000 Mr protein shares substantial sequence similarity with human prosaposin, the precursor of lysosomal saposins. Saposins are known to enhance the activity of lipid modifying enzymes presumably by solubilizing the lipids. We report here the immunolocalization of sulfated glycoprotein-1 in the cells and fluid of the male reproductive tract. The protein is present in secondary lysosomes of Sertoli cells and also in the luminal fluid of seminiferous tubules and epididymis. The highest concentrations of the protein are in seminiferous tubule fluid and rete testis fluid, while relatively low amounts are found in cauda epididymal fluid and serum. Sulfated glycoprotein-1 is believed to be involved in degradation of lipids in residual bodies and may also assist in modification of membrane lipids during sperm maturation. 相似文献
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The male genitalia of the rock hyrax (Procavia and Heterohyrax) are described. Features of the gross anatomy and histology of the testes and male accessory organs are presented, and comparison is made between the sexually active and sexually quiescent animal.
Microscopical observations have revealed the main area of storage of spermatozoa in this mammal and partly on these grounds, a new nomenclature for different parts of the reproductive system is proposed. Seasonal variations in male sexual activity in these mammals are also discussed, in addition to anatomical considerations. 相似文献
Microscopical observations have revealed the main area of storage of spermatozoa in this mammal and partly on these grounds, a new nomenclature for different parts of the reproductive system is proposed. Seasonal variations in male sexual activity in these mammals are also discussed, in addition to anatomical considerations. 相似文献
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Thyrotropin-releasing hormone and a homologous peptide in the male rat reproductive system 总被引:2,自引:0,他引:2
A E Pekary N V Meyer C Vaillant J M Hershman 《Biochemical and biophysical research communications》1980,95(3):993-1000
TRH and a TRH-like peptide have been shown to occur throughout the male rat reproductive system by TRH radioimmunoassay, SP-Sephadex C25 cation exchange chromatography, high pressure liquid chromatography and parallel line analysis. The total concentration of TRH and TRH-like peptide was highest in the prostate followed by the testis, cauda epididymis and seminal vesicles. Dilution curves for extracts of prostate, testis and seminal vesicles were parallel with TRH while the corresponding curve for epididymis was nonparallel. 相似文献
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Photoperiod and temperature affect reproductive and nonreproductive functions in male prairie voles (Microtus ochrogaster) 总被引:1,自引:0,他引:1
Adult male prairie voles (Microtus ochrogaster) were housed for 10 wk and exposed to long (16L:8D) or short (8L:16D) photoperiods at 21 degrees or 5 degrees C. Maintenance in short day lengths reduced testicular, epididymal, and seminal vesicle mass and also significantly depressed spermatogenic activity. Cold ambient temperature further suppressed gonadal size in voles exposed to short days. Several pelage characteristics were affected by photoperiod, but not by temperature. Increased fur density, fur depth, and length of guard hair and underhair were observed in voles exposed to short days. Intrascapular brown fat and gonadal fat pad mass as well as body mass were significantly less in voles housed in cold temperatures than in voles exposed to warm ambient temperatures; photoperiod did not affect these parameters. Approximately 30% of the male voles exposed to short days maintained their reproductive systems, yet they clearly processed photoperiodic information; all short-day males, regardless of reproductive condition, had comparable winter pelage development. Our results suggest that in prairie voles, photoperiod may be a predictive cue for reproductive function in nature; however, it appears that pelage development is a more obligatory response to photoperiod than is reproduction. 相似文献
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Individuals of numerous species limit energy expenditure during winter by inhibiting reproduction and other nonessential functions. To time these adaptations appropriately with the annual cycle, animals rely on environmental cues that predict, well in advance, the onset of winter. The most commonly studied environmental factor that animals use to time reproduction is photoperiod. Rodents housed in short photoperiods in the laboratory or in naturally declining day lengths exhibit pronounced alterations in reproductive function concomitant with alterations in the hypothalamic gonadotropin-releasing hormone neuronal system. Because animals in their natural environment use factors in addition to photoperiod to time reproduction, the present study sought to determine the independent effects of photoperiod and temperature, as well as the interaction between these factors, on reproductive parameters and the GnRH neuronal system. Male prairie voles were housed in either long (LD 16:8) or short (LD 8:16) day lengths for 10 weeks. Animals in each photoperiod were further subdivided into groups housed in either mild (i.e., 20 degrees C) or low (i.e., 8 degrees C) temperatures. As shown with immunohistochemistry, voles that underwent gonadal regression in response to short photoperiods and long-day voles housed in low temperatures (and maintained large gonads) exhibit higher GnRH-immunoreactive (GnRH-ir) neuron numbers in the preoptic area/anterior hypothalamus (POA/AH) relative to all other groups. In addition, voles that underwent gonadal regression in response to both short days and low temperatures did not exhibit an increase in GnRH-ir neuron numbers compared to long-day, mild-temperature controls. These data suggest that photoperiod and temperature interact to influence reproductive function potentially by alterations of the GnRH neuronal system. 相似文献
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Banerjee A Singh A Srivastava P Turner H Krishna A 《Birth defects research. Part B, Developmental and reproductive toxicology》2011,92(3):195-205
BACKGROUND: The purpose of this study was to investigate the effect of chronic uptake of bhang, prepared from the Cannabis sativa, on male reproductive physiology in adult male Parkes strain (P) mice. An attempt was also made to investigate the presence of cannabinoid 1 (CB1) and cannabinoid 2 (CB2) receptors, and fatty acid amide hydrolase (FAAH) in the testis and to evaluate any changes in it resulting from chronic intake of bhang in mice. METHODS AND RESULTS: Adult male mice were given bhang (3 or 6 mg/kg body weight/day) orally for 36 consecutive days. Chronic intake of bhang caused regressive changes in the testes and suppressed sperm count, viability and motility. Bhang intake also caused significant decline in circulating testosterone level due to decline in testicular 3β HSD enzyme activity. An immunohistochemical study demonstrated the presence of CB1, CB2 and FAAH in the testis of mice. The present study also showed significant variation in the CB1 and CB2 receptors and FAAH protein levels in testes of mice exposed to bhang. These suppressive effects may be due to inhibitory effect of bhang on pituitary expression of gonadotrophin releasing hormone (GnRH) I receptor protein. Treatment of testes with bhang in vitro significantly decreased testicular luteinizing hormone receptor (LHR) and FAAH expression suggesting direct action of bhang on testicular activity. CONCLUSIONS: The findings of this study thus suggest that bhang may impair fertility in male mice through alteration in the testicular endocannabinoid system and that chronic bhang exposure in humans would be predicted to alter male fertility. Birth Defects Res (Part B) 92:195–205, 2011. © 2011 Wiley‐Liss, Inc. 相似文献
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S. M. GROZEVA 《Systematic Entomology》1995,20(3):207-216
The karyotype of fourteen species of Berytidae has been investigated (ten of them in this paper). All studied Metacanthinae, except possibly Metatropis , have 2n(♂)= 14 + XY. In three examined genera of Berytinae the karyotypes are dissimilar: in Neides and Apoplymus (Berytinae) the chromosome number is 2n(♂)=14 + XY, as for Metacanthinae. In Berytinus spp. the chromosome number is very high ( B.distinguendus: 2n(♂)=30 + XY; B.clavipes: 2n(♂)=32 + XY: B.minor: 2n(♂)=40 + XY).
The structure of the male reproductive system of eleven species is studied. Apoplymus (Berytinae) has two elongate follicles per testis and two pairs of mesadenial glands (mg). Metacanthinae and Neides (Berytinae) have a single elongate follicle per testis and two pairs of mg. Berytinus spp. differ in a number of characters: follicles are shorter and wider, two in each testis; a paired and an unpaired mg.
The number and position of the abdominal trichobothria of twenty-nine species of Berytidae is discussed.
The ancestral condition of the three examined characters is found in primitive genera of both Berytinae and Metacanthinae. The genus Berytinus shows a derived condition in all of these characters. 相似文献
The structure of the male reproductive system of eleven species is studied. Apoplymus (Berytinae) has two elongate follicles per testis and two pairs of mesadenial glands (mg). Metacanthinae and Neides (Berytinae) have a single elongate follicle per testis and two pairs of mg. Berytinus spp. differ in a number of characters: follicles are shorter and wider, two in each testis; a paired and an unpaired mg.
The number and position of the abdominal trichobothria of twenty-nine species of Berytidae is discussed.
The ancestral condition of the three examined characters is found in primitive genera of both Berytinae and Metacanthinae. The genus Berytinus shows a derived condition in all of these characters. 相似文献
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Vereshchako GG Khodosovskaia AM Konoplia EF 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2003,43(1):71-74
The effects of prolonged irradiation at accumulated doses from 0.5 to 6.0 Gy (dose rate 3.03 cGy/day) on reproductive organs' weight (testes, epididymises, seminal vesicles, prostate) of male rats starting from the early ontogenetic period were studied. On the first day after the irradiation with 1.0 Gy dose a significant loss of the weight in testes and epididymises was revealed. This leaded to the marked atrophy of the organs with the increase of the radiation dose. Long-term restoration of the weight of testes and epididimyses was registered. It was not completed during three months after radiation exposure at 2.0 Gy and higher doses for epididimyses and 4.0-6.0 Gy for testes. The respective changes in the seminal vesicles and prostate weight were less pronounced and had more complicated character. However in the distant period (30-90 days of postreatment) after exposure to 2.0 Gy these parameters were noticeably decreased. 相似文献
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We examined the affect of handling on reproductive signal expression in male threespine stickleback, Gasterosteus aculeatus. Spectrophotometric techniques have become popular methods for measuring fish color, but the measurements require handling
of the subjects in order to make measurements. Fish can undergo rapid physiological color change in response to stress. As
such, handling fish may induce color changes and measured colour may not be representative of signal appearance under non-stressful
conditions. We measured the reflectance characteristics of the opercular, ventro-lateral and dorso-lateral areas of 41 reproductive
males from Hotel Lake, BC, Canada using spectroradiometry immediately upon capture and again at 2, 3, 4, 5, 10, 15, and 20 min
post-capture. Repeated measures ANOVA revealed that post-capture handling did not affect opercular or ventro-lateral reflectance
characteristics over the 20 min period. However, we did observe a change in dorso-lateral reflectance characteristics of parental
males over the experiment. The results of our study show that handling does not influence reflective properties of two key
components of stickleback reproductive signals during a time frame that is reasonable for taking field measurements of signal
color. 相似文献
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The γ-glutamyl transpeptidase activity of the epididymis is much higher than that of the several other organs of the reproductive system of the male rat. The epididymal caput has much more activity than the epididymal cauda. Relatively low activity was found in spermatozoa. The enzyme is present in the epididymal fluid in a particulate form suggesting that it originates from membranes of epididymal epithelial cells. The epididymal caput exhibits high γ-glutamylcysteine synthetase activity indicating an active γ-glutamyl ycle in this tissue, which plays an important role in transport phenomena. 相似文献
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Treatment of rats for 4 weeks with phenobarbital (PB) did not inhibit the growth of the seminal vesicles, nor did it affect the biosynthesis of testosterone by testis microsomes. Moreover, neither the concentration of cytochrome p-450 or the 17 α-hydroxylase activity in testis microsomes were affected. In contrast, treatment with 3-methylcholanthrene (3-MC) for 4 weeks markedly decreased the weights of the seminal vesicles. The decrease was probably related to an impairmant of testosterone formation in the gonads, since testosterone biosynthesis as well as the concentration of cytochrome p-450 and the activity of 17 α-hydroxylase in testis microsomes were significantly decreased in the 3-MC treated rats. No histopathological changes were seen in testes from any of the PB or 3-MC treated rats. 相似文献
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Co-expression and interaction of cubilin and megalin in the adult male rat reproductive system 总被引:5,自引:0,他引:5
Cubilin is a peripheral membrane protein that cooperates with the endocytic receptor megalin to mediate endocytosis of ligands in various polarized epithelia. Megalin is expressed in the male reproductive tract where it has been implicated in the process of sperm membrane remodeling. A potential role for cubilin in the male reproductive tract has not been explored. Using RT-PCR, we found that cubilin and megalin mRNAs are expressed in the efferent ducts, corpus and cauda epididymis, and proximal and distal vas deferens. Immunohistological analysis revealed that cubilin was expressed in nonciliated cells of the efferent ducts, principal cells of the corpus and cauda epididymis and vas deferens. Immunogold EM showed cubilin in endocytic pits, endocytic vesicles, and endosomes of these cells. The expression profile of cubilin in the male reproductive tract was coincident with that of megalin except in principal cells of the caput epididymis. Double immunogold labeling showed that cubilin and megalin co-localized within the endocytic apparatus and recycling vesicles of efferent duct cells. Neither protein was found in lysosomes. Injection of RAP, an antagonist of megalin interaction with cubilin, reduced the level of intracellular cubilin in cells of the efferent ducts and vas deferens. In conclusion, cubilin and megalin are co-expressed in cells of the epididymis and vas deferens and the endocytosis of cubilin in these tissues is dependent on megalin. Together, these findings highlight the potential for a joint endocytic role for cubilin and megalin in the male reproductive tract. 相似文献