天门冬纳米制剂对小鼠抗氧化系统的影响

目的:探讨中药天门冬及其纳米制剂对小鼠心肌线粒体中超氧化物歧化酶(SOD)活性,丙二醛(MDA)含量,肝组织中谷胱甘肽过氧化物酶(GSH-Px)活性的影响,观察纳米制剂能否更有效地改善机体的抗氧化能力.方法:采用D-半乳糖致衰老小鼠分别ig相同剂量的天门冬水提液及其纳米中药15 d,测定衰老小鼠心肌线粒体中SOD活性、MDA含量及肝组织中GSH-Px活性.结果:天门冬水提液及其纳米中药均能显著增强天门冬及其纳米中药均可提高小鼠心肌线粒体中SOD的活性(P<0.01),肝组织GSH-Px活性(P<0.01),降低心肌线粒体中MDA(P<0.05),且纳米中药的药效强于天门冬水提液的药效(P<0.01).结论:天门冬纳米制剂抗氧化能力优于天门冬超声波水提液.     

天门冬水提液及其纳米中药对衰老模型小鼠NOS、NO、LPF的影响

目的:探讨中药天门冬及其纳米化后对D-半乳糖衰老模型小鼠血清和肝的抗氧化作用.方法:采用D-半乳糖致衰老小鼠分别ig相同剂量的天门冬水提液及其纳米中药15 d,测定衰老小鼠血清中一氧化氮合酶(NOS)的活性,一氧化氮(NO)含量及肝组织中脂褐素(LP下)的含量.结果:天门冬水提波及其纳米中药均能显著增强小鼠血清中NOS的活性(P＜0.01),提高N0含量(P＜0.05),降低LPF含量(P＜0.05),且纳米中药的药效强于天门冬水提液的药效(P＜0.05).结论:天门冬水提液及其纳米中药均有抗氧化作用,且纳米中药比水提液药效更好.     

天门冬多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用

为探讨天门冬多糖对免疫功能低下小鼠的免疫调节作用,本实验建立环磷酰胺诱导免疫抑制小鼠模型。通过称重计算脾脏指数和胸腺指数;MTT法检测T、B淋巴细胞增殖反应;双抗夹心ELISA方法检测小鼠血清中IL-2和IL-4水平,测定天门冬多糖对免疫抑制小鼠免疫功能的调节作用。结果表明天门冬多糖能够提高免疫抑制小鼠的脾脏指数和胸腺指数,在ConA或者LPS刺激下提高T、B淋巴细胞增殖率,提高血清中IL-2和IL-4水平。天门冬多糖对环磷酰胺诱导的免疫抑制小鼠有一定的保护作用。     

灵芝提取液延缓皮肤衰老的实验研究

目的观察灵芝提取液延缓D-半乳糖致亚急性衰老小鼠皮肤衰老的作用。方法以D-半乳糖致亚急性衰老小鼠为模型,同时背部涂抹灵芝提取液,治疗42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、脂褐质(LF)含量、羟脯氨酸含量。结果灵芝提取液低、中、高剂量均能不同程度提高皮肤中SOD活力,降低MDA含量,减少LF含量,提高小鼠皮肤中羟脯氨酸的含量(P0.01,P0.05),且具有明显的量效关系。结论灵芝提取液具有延缓D-半乳糖所致衰老小鼠的皮肤衰老作用。     

银耳多糖对D-半乳糖致衰老模型小鼠抗氧化能力的影响

研究了银耳多糖对D-半乳糖致衰老模型小鼠抗氧化能力影响。将ICR小鼠腹腔注射D-半乳糖建立衰老模型,同时给予不同剂量的银耳多糖(TP),8周后获取小鼠心、脑,测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力以及丙二醛(MDA)含量的差异。结果:TP各剂量组SOD和GSH-Px活力高于对照组(P<0.05),且高剂量组SOD、GSH-Px活力均高于低剂量和中剂量组(P<0.05);中、高剂量TP组MDA含量低于对照组(P<0.05)且高剂量组MDA含量低于低剂量组(P<0.05)。结论:银耳多糖对于衰老模型小鼠抗氧化能力具有一定正性调节作用。     

无瓣海桑果实提取物对衰老小鼠学习 记忆能力的影响及其机制研究

无瓣海桑果实为真红树无瓣海桑的果实。研究无瓣海桑果实不同提取物对D-半乳糖所致衰老小鼠学习记忆能力影响及其作用机制。采用Morris水迷宫实验测量无瓣海桑果实不同提取物对小鼠的学习记忆能力影响,HE染色观察各组小鼠脑部神经细胞的变化情况,并测定脑组织超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力、一氧化氮(NO)含量和单胺氧化酶(MAO)活力。结果表明:与模型组相比,无瓣海桑果实不同提取物处理组小鼠在水迷宫实验中逃避潜伏期明显缩短(P0.05),目标象限停留时间明显增加(P0.05)。无瓣海桑果实不同提取物处理组小鼠脑部神经细胞损伤与模型组相比明显减少,小鼠脑部SOD酶活力和GSH-Px酶活力提高(P0.05),NO含量和MAO活力在脑部显著降低(P0.05)。无瓣海桑果实不同提取物对D-半乳糖致衰老小鼠学习记忆能力有改善作用,无瓣海桑果实不同提取物通过提高小鼠脑内源抗氧化酶(SOD、GSH-Px)活力,降低脑部NO含量和MAO活力来提高D-半乳糖致衰老小鼠的学习记忆能力。     

胎脑提取液对衰老小鼠肝细胞酶活性影响的实验研究

目的：观察胎脑提取液对衰老小鼠肝细胞琥珀酸脱氢酶(SDH)和酸性磷酸酶(ACP)活性的影响,探讨胎脑提取液的抗衰老作用。方法：选用健康昆明种小白鼠30只,随机分为3组;采用D-半乳糖制备亚急性衰老模型;酶组织化学染色观察各组小鼠肝细胞SDH和ACP的活性。结果：衰老模型组与正常对照组相比,小鼠肝细胞SDH活性明显降低,ACP活性明显升高;给药组与衰老模型组相比,小鼠肝细胞SDH活性明显升高,ACP活性明显降低。结论：胎脑提取液可以延缓肝细胞的衰老进程,具有一定的抗衰老作用。     

胎脑提取液对衰老小鼠海马神经元酶活性影响的实验研究

目的 观察胎脑提取液对衰老小鼠海马神经元酸性磷酸酶（ACP）和琥珀酸脱氢酶（SDH）活性的影响,探讨胎脑提取液的抗衰老作用。方法 选用健康昆明种小白鼠30只,随机分为3组;采用D-半乳糖制备亚急性衰老模型;酶组织化学方法结合显微图像分析。观察各组小鼠海马神经元ACP和SDH的活性。结果 衰老模型组与正常对照组相比,小鼠海马神经元ACP活性明显升高,SDH活性明显降低;给药组与衰老模型组相比,小鼠海马神经元ACP活性明显降低。SDH活性明显升高。结论 胎脑提取液可以延缓海马神经元的衰老进程,具有一定的抗衰老作用。     

土人参提取液对D-半乳糖所致衰老小鼠的抗氧化作用研究

本文研究了土人参提取液对D-半乳糖所致衰老小鼠的抗氧化作用的影响。取昆明种小白鼠50只,随机分为5组(正常对照组、衰老模型组、土人参提取液低、中、高剂量组)。除正常对照组外,其余各组每天于颈背皮下注射D-半乳糖125 mg/kg,连续42 d,造成亚急性衰老模型。在注射D-半乳糖的同时,三个不同剂量实验组每天分别灌胃土人参提取液5、10和15g/kg,正常对照组及衰老模型组给予等量生理盐水。于42 d后测定小鼠体内超氧化物歧化酶(Super Oxide Dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-PX)等酶的活性,及丙二醛(Maleic Dialdehyde,MDA)的含量。结果显示:与正常对照组相比,衰老模型组小鼠心脏、肝脏组织SOD及血清中GSH-PX活性降低(P0.01),MDA含量增加(P0.01)。与衰老模型组相比,土人参提取液低、中、高剂量组小鼠心脏、肝脏组织SOD及血清中GSH-PX活性明显升高(P0.01);中、高剂量组小鼠心脏、肝脏组织中MDA含量降低明显(P0.01),低剂量组小鼠肝脏的MDA含量明显降低(P0.01)。上述结果表明:土人参提取液对D-半乳糖所致衰老小鼠有抗衰老作用。     

E.ColiA.S1.588生产L—天门冬酰胺酶发酵工艺的研究

Ｅ·ＣｏｌｉＡ·Ｓ１．５８８生产Ｌ－天门冬酰胺酶发酵工艺的研究陈丽媛,金守满,刘薇,陆春左,元福实（辽宁省微生物研究所,朝阳１２２０００）Ｌ一天门冬酰胺酶（ＥＣ３．５．１．１）即Ｌ一天门冬酰胺酰胺基水解酶,专一催化Ｌ一天门冬酰胺水解形成Ｌ一天门冬氨酸...     

The effect of Bordetella pertussis lymphocytosis-promoting factor (LPF) on antibody response in mice: its enhancing and suppressive effects

The effect of lymphocytosis-promoting factor (LPF) on antibody response in mice was estimated under different sets of experimental conditions. Four- and 6-week-old mice were intravenously inoculated with LPF. Three days later these mice were inoculated either intraperitoneally or intravenously with sheep red blood cell (SRBC) or human serum albumin (HSA) as an antigen. The adjuvant effect of LPF was demonstrated on antibody response in 6-week old mice to intraperitoneally inoculated SRBC but not to intravenously-inoculated one. When 4-week-old mice were immunized, hemagglutinin production in response to intraperitoneally inoculated SRBC was not enhanced by LPF. In addition, a rather suppressive effect of LPF at a comparatively high dose was demonstrated on hemagglutinin production in response to intravenously inoculated SRBC. Anti-HSA production was enhanced by inoculation of LPF in any combination of the mouse age and the route of antigen administration. These findings indicate that the adjuvant effect of LPF on antibody response in mice depends upon experimental conditions: the age of mice, the quality of antigen and the route of antigen administration used for immunization.     

Effect of the lymphocytosis-stimulating factor from Bordetella pertussis on murine lymphoid and hemopoietic cells

Effect of B. pertussis lymphocytosis-promoting factor (LPF) on the lympho-hematopoietic system of mice was studied. The injection of LPF was shown to sharply enhance endogenous colony formation and to induce a severe depletion of thymus cells, reaching its maximum of day 4. Thymocytes obtained on day 2 or 3 after the injection of LPF produced a suppressive effect on endogenous colony formation. The proliferative activity of hematopoietic stem cells sharply increased under the influence of LPF, though it had no radioprotective action. On the following day after the injection of LPF a steep rise in the number of hematopoietic stem cells was observed in the blood of mice: their content increased 20-fold in comparison with the control level. These data may be important for the evaluation of the side effects of pertussis vaccine on the lympho-hematopoietic system.     

Effect of Bordetella pertussis leukocytosis (lymphocytosis)-promoting factor (LPF) on the physical lymphoepithelial-cell association studied with the use of an in vitro model of mouse thymus

The effect of highly purified leukocytosis (lymphocytosis)-promoting factor (LPF) of Bordetella pertussis on physical lymphocyte and reticuloepithelial (RE) cell association was studied in an in vitro thymus model. First, a simplified in vitro system to assess the lympho-RE-cell association was developed. A completely confluent layer of thymic RE cells was formed by cultivating trypsinized thymus cell suspensions from 2- to 7-day-old mice. When thymic lymphoid cells were seeded on this cell layer and cultivated overnight, a significant proportion of them were found underneath the RE cell layer. This physical lympho-RE-cell association was quantitated by counting the lymphoid cells underneath the RE cell layers. Second, the effect of LPF on this physical lympho-RE-cell association phenomenon was investigated. Addition of LPF to the culture markedly inhibited the formation of the lympho-RE-cell complex; that is, it inhibited the infiltration of lymphoid cells under the RE cell layer. LPF rendered a nearly maximal level of inhibitory effect at a dose of 0.1 ng/ml. Furthermore, LPF enhanced the liberation of lymphoid cells from preformed lympho-RE-cell complexes. On the other hand, LPF had no direct cytotoxic effect on lymphoid cells at doses below 1 microgram/ml. In order to investigate whether LPF produced the effect by acting on lymphoid cells, RE cells, or both, the following experiments were performed. When lymphoid cells were pretreated with LPF and added to normal RE cell layers, the lympho-RE-cell association was maximally inhibited above the dose of 1 ng/ml. Treatment of these LPF-treated lymphoid cells with anti-LPF antibodies failed to abrogate the effect of LPF. When RE cell layers were similarly pretreated with LPF and were cultivated with normal lymphoid cells, however, much higher doses of LPF, above 100 ng/ml, were required for maximal inhibition. Furthermore, treatment of these LPF-treated RE cells with anti-LPF antibodies abrogated the effect of LPF. Therefore, the apparent effect of LPF on RE cells was considered to be due to the carry-over by RE cells of LPF, which should directly act on lymphoid cells at extremely low doses. On the basis of these results, it was concluded that LPF acted directly on lymphoid cells without mediation of RE cells. These in vitro results appear to parallel the effects of LPF in vivo, where it induces a depletion of cells in the thymus. The model may be useful to study this phenomenon and the concomitant accumulation of blood lymphocytes.     

地锦粗提物对脾虚小鼠脏器指数和抗氧化能力的影响

目的探讨地锦粗提物对脾虚小鼠脏器指数和抗氧化能力的影响,为防治脾虚提供参考。方法 30只昆明小鼠随机分成3组,每组10只,即对照组、脾虚组和地锦组。脾虚组和地锦组采用泻下加劳倦方法,灌服大承气汤30 g/(kg.d),并负重游泳,对照组灌服生理盐水30 g/(kg.d),不游泳,持续60 d,然后灌胃60 d,地锦组灌胃地锦水醇浸出液30 g/(kg.d),脾虚组和对照组给予相当剂量的生理盐水。实验结束后取心、肝、脾、肺、肾,称重并计算脏器指数,测血浆过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活力,测心、肝、脾、肺、肾中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果脾虚组心指数和肺指数极显著增加,左肾指数显著增加,地锦组心指数显著增加。地锦组的血浆CAT和GSH-Px活力显著高于脾虚组,地锦组肝和脾中的SOD活力显著高于脾虚组,地锦组脾、肺MDA含量显著低于脾虚组。结论地锦粗提物可降低脾虚小鼠的脏器指数,并可提高脾虚小鼠抗氧化能力。     

Interaction of low doses of ionizing radiation and carbon tetrachloride on liver superoxide dismutase and glutathione peroxidase in mice

Male BALB/c mice were treated with intraperitoneal injections of carbon tetrachloride (CCl4) (0.2 g/kg body weight) and/or 50 R of whole-body gamma irradiation, three times per week, for 4 weeks. The effects of the treatments on superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver extracts and homogenates, and on alkaline phosphatase (ALP) in serum were investigated. A significant decrease in the SOD and GSH-Px activities in liver extracts and an increase of serum ALP of hepatic origin were found in CCl4-treated animals. In contrast, only an increase in SOD activity was observed in liver homogenates after the combined treatment.     

Selenium modifies glutathione peroxidase activity and glutathione concentration in mice exposed to ozone-provoked oxidative stress

The aim of this study was to show the direct effect of selenium on glutathione peroxidase (GSH-Px) activity and GSH/GSSG concentrations in 3- and 6-month-old mice. An ozone-oxygen mixture was used to provoke an oxygen stress. To measure the Se-effect mice were gavaged with sodium selenite. GSH-Px activity and total glutathione concentrations were determined in serum and in the postnuclear fraction of liver and lungs. Additionally glutathione concentrations were determined in whole blood. Both ozone and selenium, administered separately, reduced GSH-Px activity in lungs of 6-month-old animals, while in young mice an opposite effect of Se was observed. Ozone administered jointly with Se did not influence GSH-Px activity in 6-month-old mice, while in young, 3-month-old mice, a stimulatory effect in lungs was observed. There were no significant changes in GSH-Px activity in the liver of 6-month-old mice, but the stimulatory effect occurred in young mice treated with Se and Se & ozone jointly. In young mice, ozone (also ozone with Se) augmented glutathione concentrations. The response to ozone and selenium strictly depended on age and the antagonism between selenium and ozone was observed only in a few cases.     

三七总皂甙和银杏叶提取物预防急性氧中毒的实验研究

目的:研究三七总皂甙和银杏叶提取物对急性氧中毒的预防作用及其可能机制.方法:分别给小鼠连续腹腔注射三七总皂甙和银杏叶提取物5 d后,在500kPa高压氧中暴露60 min,观察惊厥潜伏期、惊厥次数、惊厥间隔时间等指标;另外测定高压氧暴露15 min后脑组织中活性氧单位、脂质过氧化物、一氧化氮、谷胱甘肽的含量和过氧化氢酶、谷胱甘肽过氧化物酶、单胺氧化酶的活性.结果:三七总皂甙和银杏叶提取物可以明显延长氧惊厥潜伏期和惊厥间隔时间,减少惊厥次数;降低高压氧暴露后脑组织中脂质过氧化物、一氧化氮的含量,使活性氧单位、谷胱甘肽含量和谷胱甘肽过氧化物酶活性保持在较高的水平;对过氧化氢酶和单胺氧化酶活性的影响则不显著.结论:三七总皂甙和银杏叶提取物可以有效预防急性氧中毒,其机制可能与它们的抗氧化活性有关.