樟科植物花粉形态研究

樟科植物为我国南方常绿阔叶林重要组成成分,我国约有20属420余种。过去对于樟科花粉形态的研究仅限于在光学显微镜下进行观察记载,以台湾学者王仁礼的研究最为全面,共记载了台湾产14属40余种的花粉形态。本文作者以国产樟科为主,应用扫描电镜及光学显微镜对该科花粉形态进行了更为全面系统的研究,共观察记载了22属150余种,对各属的花粉形态特征均进行了描述;首次报道了樟科花粉有小穿孔的存在,并依据花粉的形状、外壁纹饰及小穿孔存在与否将樟科花粉划分为7个类型,即:1．厚壳桂型(Cryptocarya,Neocimamomum);2．檬果樟型(Caryodaphnopsis);3．木姜子型(Litsea,Lindera,Neolitsea);4．月桂型(Laurus);5．鳄梨型(Persea,Machilus,Syndiclis,Nothaphoebe, Beilschemiedia,Phoebe,Alseodaphne,Octea);6．檫木型(Sassafras,Cinnamomum,Umbellularia,Actinodaphne,Dehaasia);7．无根藤型(Cassytha)。文中还讨论了樟科花粉的多样性、有关属之间的亲缘关系和分类问题以及同邻近的肉豆蔻科、莲叶桐科的关系。     

泽泻科的花粉形态研究

本文对泽泻科11属27种代表植物的花粉进行了光学显微镜、扫描电镜和透射电镜观察。在系统描述了该科及各属植物花粉形态的基础上,将泽泻科植物的花粉划分为3种类型,即少果泽苔草型、慈菇型和泽泻型。根据花粉形态特征的比较,并依据泽泻科植物祖先类群的花粉具有船形、具单沟萌发孔、花粉外壁具明显的刺状纹饰、覆盖层完整无通道等特征,作者认为泽泻科植物花粉形态的如下演化趋势是明显的：由船形演化为卵球形、球形和多面体球形;由单沟萌发孔经过一无孔的中间类型演化为散孔类型;孔膜由光滑演化为具颗粒和小刺;萌发孔不内陷进化到内陷;花粉粒外壁的刺状纹饰逐渐过渡为颗粒状纹饰或者消失,以及覆盖层由无通道到具细通道和通道。     

中国罗汉松科花粉形态研究

本文用光学显微镜,结合扫描电镜和透射电镜对我国罗汉松科podocarpaceae植物花粉外壁的细微结构做了深入的研究,对比了我国仅有的两属花粉的形态特征,同时与形态上相似的松科花粉进行了比较,指出了它们之间的区别。     

凤仙花属花粉形态及其分类学意义

本文作者首次观察了峨眉山凤仙花属Impatiens 13个野生种的花粉形态,发现3沟和4沟类型 花粉均出现在这一地区。其中3沟花粉与同科另一属水角属Hydrocera的花粉十分相似。同时注意到 花粉形态与其花的形态在某些方面具相关性,结合地理分布和生长习性等特性,得出以下结论：1．凤仙 花属3沟型花粉所指示的植物类群为一自然类群,并与水角属具有较密切的亲缘关系;2．凤仙花属4沟型花粉常具种水平上的差异,对属内类群划分具有系统学意义。     

蜘蛛抱蛋亚族的花粉形态

本文研究并报道了百合科蜘蛛抱蛋亚族的花粉形态,主要结论如下：1．开口箭属含两种花粉类型即：(1)单槽、椭圆形;(2)无萌发孔、球形。具单槽、椭圆形花粉的种类含单型性核型,花形较小,柱头小而花柱短,雄蕊高于柱头;无萌发孔、球形花粉的种类则为单型性核型,花形较大,柱头常高度膨大,雄蕊常着生在花被筒基部。2．万年青属花粉为椭圆形,具单槽;蜘蛛抱蛋属花粉则为球形,无萌发孔;开口箭属花粉一部分与万年青属相似,另一部分则与蜘蛛抱蛋属雷同。3．蜘蛛抱蛋亚族中最原始的花粉类型为单槽椭圆形,具孔状或网状外壁纹饰。球形,无萌发孔,外壁具皱波状、瘤状或芽孢状纹饰的花粉为派生类型。4．蜘蛛抱蛋亚族种下花粉形状、萌发孔类型和外壁纹饰基本稳定,可作为分种特征。     

湖北省泽泻科、水鳖科、眼子菜科及茨藻科植物花粉形态研究

本文利用光镜及扫描电镜对湖北省泽泻科、水鳖科、眼子菜科及茨藻科11属29种1变种1变型植物(另加采于湛江的软骨草)的花粉形态进行了研究,发现泽泻科植物花粉具多个圆形萌发孔,外壁表面为小刺状纹饰;茨藻科植物花粉具远极单槽,表面为绉波状纹饰;眼子菜科及本文研究的水鳖科植物花粉均无萌发孔,分别具网状和小刺状饰纹饰。1.茨藻科植物花粉最原始,泽泻科花粉较进化,眼子菜科花粉较水鳖科花粉进化;2.泽泻属与泽苔草属花粉较慈姑属花粉原始;3.鞘叶眼子菜亚屈花粉较眼子菜亚属的花粉处于更高演化阶段;4.多孔茨藻花粉在该科中最原始。本文工作尚对易变形水生植物花粉形态研究方法进行了尝试。     

中国蓼属叉分蓼组植物花粉形态的研究

采用光学显微镜和扫描电镜对中国蓼属叉分蓼组20种3变种的花粉形态进行了观察和研究。结果表明其花粉形态大多数为近球形至近长球形,少数为扁球形或长球形;花粉大小为20.4～44.0µm×17.0～34.0µm：从萌发孔看,有3沟、3 孔沟、多沟、散沟;外壁纹饰为微刺—穴状、刺状、粗网状、皱块状。据此,该组花粉可划分为5种类型,即叉分蓼型(Aconogonon-type)、钟花蓼型(Campanulatum-type)、大连线冰岛蓼型(Forrestii-type)、西伯利亚蓼(Sibiricum-type)及多穗蓼型(Polystachyum-type),编制了这些花粉类型检索表。叉分蓼型花粉的主要特征是具3沟,外壁纹饰为微刺-穴状,此种类型的植物有14种2变种。钟花蓼型花粉的主要特征是具6散沟,外壁纹饰为微刺-穴状,此种类型的植物有钟花蓼和绒毛钟花蓼。西伯利亚蓼型花粉的主要特征是具3孔沟,外壁纹饰为皱块状,此种类型的植物有西伯利亚蓼。多穗蓼型花粉的主要特征是具6(~8)多沟,外壁纹饰为粗网状,此种类型的植物有松林蓼及多穗蓼。大连线冰岛蓼型花粉的主要特征是具散沟,外壁纹饰为显著的长刺状,此种类型的植物有大铜钱叶蓼及铜钱叶蓼,结果表明叉分蓼组的花粉形态具有重要的分类学意义,研究结果支持将叉分蓼组上升为属的等级,也支持Knorringia的属的地位,大铜钱叶蓼和铜钱叶蓼应移入Koenigia属中,而松林蓼和多穗蓼仍保留在蓼属中。     

中国悬钩子属花粉形态观察

通过扫描电镜观察了中国悬钩子属7组23亚组共103个种的花粉形态。本属花粉为圆球形、近 球形、长球形和超长球形,少数为菱形和六边形,具三孔沟,大小多为16.0～38.Oμm×12.0～30.0μm,外壁纹饰可分为皱波型、条纹型、脑纹型和穿孔网型等类型,进而可分为皱波-近光滑状、皱波-条纹状、皱波-穿孔状、条纹状、条纹-穿孔状、条纹-网状、脑纹状、脑纹-穿孔状、穿孔状、穿孔-网状及网状等11个亚型。分析结果认为,悬钩子属的花粉形态特征对该属种的区分有一定的参考价值,但要合理地阐释其系统发育意义,仍需积累更多的信息。     

栝楼属花粉形态研究及其在分类学上的意义

本文收集了国内外栝楼属Trichosanthes 31种1变种,在光学显微镜和扫描电镜下,进行了花粉粒形态的比较观察。该属花粉粒为3孔沟型,外壁表面纹饰可分为四个类型,即：疣状或皱波状(小苞组),粗网状(大苞组),细网状或光滑(叶苞组),近光滑或皱波状(王瓜组)。这四个类型的划分与植物形态分类基本一致,可作为分组及分种的依据之一。花粉特征支持将叶苞组分为叶苞亚组和柔毛亚组,如叶苞亚组有明显的沟,而柔毛亚组没有。     

中国天南星科花粉形态的研究

利用扫描电镜对天南星科Araceae22属28种(除Arum maculatum产自德国外,其余均产自中国) 及菖蒲科Acoraceae 1属2种植物的花粉形态进行了观察。结果显示天南星科花粉形态在科内变异很 大。花粉粒形状从球形、近球形、椭球形到扁球形和橄榄形;萌发孔类型有散孔型、具薄壁区型、环沟型 或无萌发孔;外壁纹饰为小穴状、网状、肋条状、条纹状、疣状、具刺或光滑。主要依据花粉形态方面的证 据探讨了崖角藤属Rhaphidophora、麒麟叶属Epipremnam 、龟背竹属Monstera 3属的属间关系以及犁头尖属Typhonium属下分类中存在的一些问题。     

南水青冈属花粉形态类型及其分类学意义

采用光学显微镜、扫描电镜及透射电镜,对分布于南美洲、澳大利亚、新西兰、新喀里多尼亚岛和新几内亚岛的南水青冈属Nothofagus26种2变种,包括33份花粉材料,进行了观察测定及超微结构分析研究。通过对花粉的形状、大小、萌发孔特征的比较分析,得出以下结果：南水青冈属花粉粒为扁球形至超扁球形;萌发孔具5～8短沟,稀为4沟或9沟,沟缘普遍加厚;表面具不同形状、大小和疏密的刺状纹饰,花粉外壁超微结构与壳斗科(狭义)其它属迥然不同。研究结果表明,该类群的花粉在形状、大小、沟的数目和沟缘加厚等特征上亦具较大差异,据此可划分为3种类型,即N ．brassii型,N．menziesii型和N．fusca型。     

Pollen morphology of the genus Nothofagus and its taxonomic significance

The present paper describes the pollen morphology of 26 species and 2 varieties in Nothofagus from Australia, New Zealand, New Caledonia, New Guinea and South America. Pollen grains were all examined with light microscope (LM), scanning electron microscope ( SEM ) and transmission electron microscope(TEM). A comparative analysis of pollen exine ultrastructure was made for some species. The results are summarised as follows: Pollen grains are oblate to peroblate, 5～8-short-colpate, rarely 4- or 9-colpate; colpi generally thickened at margins; pollen surface spinulose. The exine ultrastructure of Nothofagus differs considerably from that of the other genera in the Fagaceae. The pollen grains of the species examined here show great differences in shape, size, colpal number and characteristics of colpi at margins and could be divided into three distinct types, i.e. N. brassii type; N. menziesii type and N. fusca type.     

五味子科植物花形态及其系统学意义

用光镜和扫描电镜观察了五味子科Ｓｃｈｉｓａｎｄｒａｃｅａｅ２３种、６变种和１变型的９１份花粉的形态。其中５ 、４变种和１变型的花粉形态为首次报道。该科花粉为单花粉,异极,辐射对称,扁球形至超扁球形,其萌发沟的排列格局在被子植物中是独一无二的。若按萌发沟的数目划分,该科花粉可分为３沟型和６沟型两个类型。外壁为网状纹饰,网眼的大小和网脊的宽度与花的形态有一定相关性。研究结果不支持Ｐｒａｇｌｏｗｓｋｉ对     

Pollen morphology of the Schisandraceae and its systematic significance

Pollen morphology of 91 samples, representing 23 species, 6 varieties and 1 form of the Schisandraceae, was examined under light microscope (LM) and scanning electron microscope (SEM). Palynological data of 5 species, 4 varieties and 1 form are reported for the first time. The pollen grains are monads, heteropolar, radiosymmetric and oblate to peroblate in shape. Arrangement of their colpi is unique in angiosperms. The pollen grains can be divided into two types according to the number of their clopi: the tricolpate pollen type and the hexacolpate pollen type. The exine sculpturing is reticulate. The size of luminae and the breadth of muri are correlated with the floral morphology of the hisandraceae to a certain extent. Our results do not support the division of the pollen grains of the Schisandraceae into 4 types according to the size of inae as proposed by Praglowski. There is also still room of discussion about his conclusion that the pole where the three longer colpi converge is the distal pole. The evolutionary trends of the pollen grains of the Schisandraceae seem to be from fewer to more in the number of colpi, and from smaller to larger in the size of luminae. The view that Kadsura is more primitive than Schisnadra is not supported by palynological data; on the contrary, Kadsura seems to be more advanced than Schisandra at the general level of evolution. The two genera may be closely related, and might have originated from a common ancestor and then evolved parallelly along two different routes.     

Pollen morphology in Asiatic species ofTrillium

Pollen grains of five species and two interspecific hybrids ofTrillium from eastern Asia have been examined with scanning electron and transmission electron microscopy. All species from eastern Asia, excludingT. govanianum, are characterized by inaperturate pollen and an unstratified and granulated exine structure. Pollen of the Himalayan species,T. govanianum, is, however, monocolpate and intectate. The palynological evidence suggests that the Asiatic species, excludingT. govanianum, are closely related to each other and toT. erectum in North America, andT. govanianum is independent of the other species in the genus.     

Pollen morphology of the genus Rubus from China

Pollen morphology of 103 species belonging to twenty-three subsections of seven sections of Rubus L. was examined with SEM. The pollen grains are usually 3-colporate, spheroidal, subspheroidal, prolate and perprolate, though occasionally rhombic and hexagonal, 16.0~38.0 μm × 12.0~30.0 μum in size. The exine ornamentation can be divided into rugulate, striate, cerebroid and perforate-reticulate types, which include 11 subtypes: rugulate-subpsilate, rugulate-striate, rugulate-perforate, striate, striate-perforate, striate-reticulate, cerebroid, cerebroid-perforate, perforate, perforate-reticulate and reticulate. The pollen morphology is of some significance for a better classification of the genus Rubus, but seems to be of little help for a better understanding of thephylogeny of the genus.     

Pollen morphology of certain species of the family Lamiaceae in Saudi Arabia

The intention of the present wok is to provide an account on the pollen morphological features of Lamiaceae in Saudi Arabia as a basis for future studies of Lamiaceae pollens in the region. Pollen morphology of 20 species belong to 16 genera of the Lamiaceae has been investigated using Light Microscope (LM) and Scanning Electron Microscope (SEM). The study revealed that the pollen grains were characterized by 3-zonocolpate or 6-zonocolpate. Size of the pollen is variable between the genera but not among the species of the same genus. The surface pattern of the exine varies from fine reticulate, rough reticulate, mega-reticulate, reticulate-perforate, bireticulate-perforate or granulate, leading to 6 types of pollen grains. These variations revealed by this study implies that pollen Morphology may be of significant value sharing in solving problems in the classification of Lamiaceae members. A Key to the species, based on the morphological features of pollen grains, is also provided.     

水鳖科9属15种植物花粉形态的研究

应用光学显微镜、扫描电镜和透射电镜对水鳖科Hydrocharitaceae 9属15种植物的花粉形态进行 了观察。水鳖科植物花粉为圆球形至近椭球形,无萌发孔或偶为单沟萌发孔,外壁纹饰通常为小刺状纹 饰,刺密集或稀疏,花粉表面具瘤状、疣状、颗粒状、皱波状突起或光滑。外壁由覆盖层、柱状层和基层组 成。覆盖层厚或较薄,柱状层小柱发育不明显,基层薄。水鳖科植物在花粉大小、纹饰类型、刺的长短、 密度、形态、萌发孔的有无以及花粉壁的结构等方面表现出了较为明显的差异,这些特征对探讨类群间 关系具有较重要意义。由于黑藻属Hydrilla和Stratiotes属花粉较为特殊,支持将它们各自作为一个独立 的族处理。水鳖科植物花粉外壁纹饰和结构特点表明该科与水雍科Aponogetonaceae、泽泻科Alismataceae 和花蔺科Butomaceae等近缘,而该科植物花粉大多无萌发孔等则反应了该科与茨藻目Najadales植物有密切联系。