The structural basis for tight control of PP2A methylation and function by LCMT-1

1. Download : Download high-res image (287KB)
2. Download : Download full-size image

Highlights? LCMT-1 makes extensive contacts to PP2A active site for methylation of PP2A tail ? PP2A methylation is stimulated by phosphatase activation, hampered by inactivation ? LCMT-1 would facilitate efficient transition of activated PP2A to holoenzymes ? A high-affinity dnLCMT-1 mutant attenuates the cell cycle without causing cell death     

The Structure of the Tiam1 PDZ Domain/ Phospho-Syndecan1 Complex Reveals a Ligand Conformation that Modulates Protein Dynamics

1. Download : Download high-res image (206KB)
2. Download : Download full-size image

Highlights? The Tiam1 PDZ domain preferentially binds a subset of syndecan (SDC) proteins ? The PDZ domain/phospho-SDC1 structure reveals a phosphoryl binding pocket ? Ligand specificity and phosphorylation regulate PDZ/SDC interactions and signaling ? Tiam1 PDZ domain dynamics are ligand-dependent and regulated by phosphorylation     

Functional implications from the Cid1 poly(U) polymerase crystal structure

1. Download : Download high-res image (313KB)
2. Download : Download full-size image

Highlights? Our structure identifies the UTP-binding pocket of the Cid1 protein ? The atomic model suggests a central role for the helix 4 ? We suggest that the NRM loop is blocked after triphosphate recognition ? We identify several loops likely involved in the RNA primer association/stabilization     

The Glove-like Structure of the Conserved Membrane Protein TatC Provides Insight into Signal Sequence Recognition in Twin-Arginine Translocation

1. Download : Download high-res image (316KB)
2. Download : Download full-size image

Highlights? Crystal structures of TatC from the thermophile Aquifex aeolicus are presented ? The architecture of TatC generates a glove-shaped pocket buried in the bilayer ? Molecular dynamics reveal destabilization of the membrane around the pocket ? Correlation to biochemical results suggest the signal sequence binds in this pocket     

Redox-Dependent Control of FOXO/DAF-16 by Transportin-1

1. Download : Download high-res image (206KB)
2. Download : Download full-size image

Highlights? FOXO forms redox-sensitive, disulfide-dependent complexes with several proteins ? Transportin-1 binds to FOXO via a disulfide and regulates its nuclear localization ? Redox and insulin signaling govern FOXO nuclear localization via distinct pathways ? Redox control of longevity protein FOXO/DAF-16 is evolutionarily conserved     

The PP2A Inhibitor I2PP2A Is Essential for Sister Chromatid Segregation in Oocyte Meiosis II

1. Download : Download high-res image (90KB)
2. Download : Download full-size image

Highlights? PP2A colocalizes with Rec8 in mouse oocyte meiosis II ? The PP2A inhibitor I2PP2A is expressed in ascidian and mouse oocyte meiosis ? I2PP2A colocalizes with PP2A in meiosis II, independently of bipolar attachment ? I2PP2A is required for sister separation in mouse oocyte meiosis II     

Inhibition of protein phosphatase 2A activity by PI3Kγ regulates β-adrenergic receptor function

1. Download : Download high-res image (376KB)
2. Download : Download full-size image

Highlights? PI3Kγ inhibits βAR resensitization by regulating PP2A activity ? PI3Kγ inhibits PP2A activity by phosphorylating I2PP2A on Serine 9 and 93 ? Phosphorylation of I2PP2A leads to its agonist-mediated interaction with PP2A ? siRNA-targeted depletion of I2PP2A results in βAR resensitization     

PP2A phosphatase acts upon SAS-5 to ensure centriole formation in C. elegans embryos

1. Download : Download high-res image (195KB)
2. Download : Download full-size image

Highlights? PP2A phosphatase is essential for centriole formation in C. elegans ? PP2A subunits genetically and physically interact with the SAS-5/SAS-6 complex ? PP2A-mediated dephosphorylation of SAS-5 is required for SAS-5/SAS-6 centriolar targeting ? Human PP2A is required for HsSAS-6 centriolar targeting and centriole formation     

Selective and specific inhibition of the plasmodium falciparum lysyl-tRNA synthetase by the fungal secondary metabolite cladosporin

1. Download : Download high-res image (307KB)
2. Download : Download full-size image

Highlights? The fungal secondary metabolite Cladosporin inhibits liver- and blood-stage malaria parasites ? Cladosporin specifically targets lysyl-tRNA synthetase (Krs1) ? Cladosporin is >100-fold more potent against parasite Krs1 relative to the human enzyme ? Two amino acids in the Krs1 ATP-binding pocket confer species-selective inhibition     

Molecular mechanism for inhibition of g protein-coupled receptor kinase 2 by a selective RNA aptamer

1. Download : Download high-res image (622KB)
2. Download : Download full-size image

Highlights? C13 RNA aptamer variants stabilize GRK2 in a unique and remodeled conformation ? The aptamer positions an adenine nucleotide into the ATP-binding pocket of GRK2 ? Basic residues on an exterior surface of GRK2 facilitate aptamer binding ? The terminal stem of the aptamer indirectly contributes to affinity     

A Predictive Computational Model of the Dynamic 3D Interphase Yeast Nucleus

1. Download : Download high-res image (367KB)
2. Download : Download full-size image

Highlights? We describe a predictive computational model of dynamic chromosomes in the yeast nucleus ? The model quantitatively recapitulates experimental data on nuclear organization ? The model predicts nuclear reorganization in response to treatment by rapamycin ? Large-scale nuclear organization is dominated by unspecific effects of crowded polymers     

β-Catenin-Driven Binary Fate Specification Segregates Germ Layers in Ascidian Embryos

1. Download : Download high-res image (134KB)
2. Download : Download full-size image

Highlights? β-catenin nuclear asymmetry after animal-vegetal-oriented cell divisions ? β-catenin nuclear asymmetry drives binary cell fate choices ? Combinatorial codes of nuclear β-catenin activation segregate ascidian germ layers ? Nuclear β-catenin ON-to-OFF activity is required for marginal mesoderm formation     

Cap-assisted internal initiation of translation of histone H4

1. Download : Download high-res image (186KB)
2. Download : Download full-size image

Highlights? Two structural elements of histone H4 mRNA drive translation initiation ? Ribosome is tethered by an eIF4E-binding site located in the coding region ? A RNA three-way helix junction element positions ribosome on the start codon ? The m⁷G cap-binding pocket formed by the mRNA controls histone translation     

Protein Phosphatase 2A Catalytic Subunit α Plays a MyD88-Dependent,Central Role in the Gene-Specific Regulation of Endotoxin Tolerance

1. Download : Download full-size image

Highlights? PP2Ac is constitutively activated and targets MyD88 in LPS-tolerized macrophages ? Constitutively active PP2Ac shifts a proinflammatory MyD88 to its prosurvival mode ? Constitutively active PP2Ac reprograms gene-specific chromatin modification landscape ? Constitutively active PP2Ac broadly defines ET at both signaling and epigenetic levels     

Dynamic Response Diversity of NFAT Isoforms in Individual Living Cells

1. Download : Download high-res image (244KB)
2. Download : Download full-size image

Highlights? Two isoforms of the NFAT proteins possess distinct and diverse dynamic responses ? NFAT4 shows fast, stochastic, and frequency-modulated bursts of nuclear localization ? NFAT1 shows a slow, continuous, and amplitude-modulated nuclear localization ? Dynamic diversity can endow cells with enhanced signal processing capabilities     

Mechanism for KRIT1 Release of ICAP1-Mediated Suppression of Integrin Activation

1. Download : Download high-res image (110KB)
2. Download : Download full-size image

Highlights? Cocrystal structure of KRIT1 with ICAP1 shows a bidentate interaction interface ? Cocrystal structure of ICAP1 with integrin β1 shows a PTB-peptide interaction ? KRIT1 antagonizes ICAP1-modulated integrin activation by the bidentate interaction ? KRIT1 contains a previously undescribed N-terminal Nudix domain     

An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 Module Is an Essential Early Component of Chitin-Induced Rice Immunity

1. Download : Download high-res image (195KB)
2. Download : Download full-size image

Highlights? Chitin induces rapid activation of the rice small GTPase OsRac1 at the plasma membrane ? OsRacGEF1 interacts with and acts as a guanine nucleotide exchange factor for OsRac1 ? In response to chitin, OsRacGEF1 is activated via phosphorylation by OsCERK1 ? OsRacGEF1 is required for chitin-driven immunity and resistance to rice blast fungus     

POT1-TPP1 Regulates Telomeric Overhang Structural Dynamics

1. Download : Download high-res image (114KB)
2. Download : Download full-size image

Highlights? POT1 binds the telomere overhang sequentially one OB fold at a time ? POT1 binding exhibits 3′ to 5′ directionality ? POT1-TPP1N induces dynamic folding and unfolding of telomeric overhang ? POT1-TPP1N slides back and forth on the telomeric overhang     

SIP1 Mediates Cell-Fate Decisions between Neuroectoderm and Mesendoderm in Human Pluripotent Stem Cells

1. Download : Download high-res image (184KB)
2. Download : Download full-size image

Highlights? Smad-interacting protein 1 (SIP1) regulates hESC differentiation ? SIP1 upregulation promotes neuroectodermal differentiation ? SIP1 inhibits mesendodermal and endodermal differentiation ? SMAD2/3 and NANOG/OCT4/SOX2 cooperatively regulate SIP1 expression